• Title/Summary/Keyword: Functional screen

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Protein Function Finding Systems through Domain Analysis on Protein Hub Network (단백질 허브 네트워크에서 도메인분석을 통한 단백질 기능발견 시스템)

  • Kang, Tae-Ho;Ryu, Jea-Woon;Kim, Hak-Yong;Yoo, Jae-Soo
    • The Journal of the Korea Contents Association
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    • v.8 no.1
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    • pp.259-271
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    • 2008
  • We propose a protein function finding algorithm that is able to predict specific molecular function for unannotated proteins through domain analysis from protein-protein network. To do this, we first construct protein-protein interaction(PPI) network in Saccharomyces cerevisiae from MIPS databases. The PPI network(proteins; 3,637, interactions; 10,391) shows the characteristics of a scale-free network and a hierarchical network that proteins with a number of interactions occur in small and the inherent modularity of protein clusters. Protein-protein interaction databases obtained from a Y2H(Yeast Two Hybrid) screen or a composite data set include random false positives. To filter the database, we reconstruct the PPI networks based on the cellular localization. And then we analyze Hub proteins and the network structure in the reconstructed network and define structural modules from the network. We analyze protein domains from the structural modules and derive functional modules from them. From the derived functional modules with high certainty, we find tentative functions for unannotated proteins.

Screen of Functional Activity of Polysaccharide and Glycosaminoglycan from Sea Hare (Aplysia kurodai) by Cell Line (세포주를 이용한 군소 다당류와 Glycosaminoglycan의 기능성 검색)

  • Hong, Yu-Mi;Park, Si-Hyang;Yoon, Bo-Yeong;Choi, Byeong-Dai;Choi, Yeung-Joon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.1
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    • pp.14-19
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    • 2011
  • In this study, we extracted a whole polysaccharide fraction from the sea hare, Aplysia kurodai, and screened its functional properties using cell lines. The functionalities of polysaccharide and glycosaminoglycan (GAG) were investigated with RAW 264.7 cell lines. The crude polysaccharides and GAG purified DEAE-Sepharose chromatography did not show the toxicity on RAW 264.7 cell line in the range of $10\sim200{\mu}g$/mL, whereas they increased the cell growth rate. The crude polysaccharides and purified GAG also increased the production of NO, interleukin-6 and tumor necrosis factor-$\alpha$ on RAW 264.7 cell. Particularly, the purified GAG inhibited the proliferation of stomach cancer cell line, AGS, up to 40% for 72 hr incubation, but not the intestinal epithelial IEC-6 cell lines.

Relationship Between Titleist Performance Institute Level 1 Test and the Performance of Professional Golf Players (프로 골프선수의 TPI Level 1 Test Score에 따른 경기력 수준 분석)

  • Kim, Jae-Eun;Do, Kwang-Sun;Kim, Cheong
    • Journal of The Korean Society of Integrative Medicine
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    • v.10 no.2
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    • pp.27-35
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    • 2022
  • Purpose : The purpose of this study is to analyze the correlation between the TPI Level 1 test and the performance of KPGA professional golf players. Methods : In 2019, 30 KPGA golf players attempted in the TPI Level 1 test. Their performance was then compared with the test based on the players' aggregated official records on the KPGA website, The most meaningful prize money ranking, average driving distance, fairway landing rate, and average number of putts were considered to evaluate their performance. Additionally, to obtain the average value of the players' accumulated records, the period from the first game in March 2019 to the end of October was considered. Results : The criterion for the difference between the upper group and the lower group was set based on the 9 points of the TPI Level 1 test, which showed the most significant difference. The prize money ranking stood at 63.00±51.77 in the upper group and 113.92±68.79 in the lower group in the TPI Level 1 test, the difference was significantly higher (p<.05) for the upper group (p<.05). The average driving distance was 286.15±10.06 yds for the upper group and 277.39±8.49 yds for the lower group, group, with the driving distance significantly higher in the upper group (p<.05). Further, the average number of putts for the upper group was 1.81±.02 and 1.85±.04 for the lower group, indicating a significant difference. Conclusion : A higher TPI Level 1 test score is likely to have a positive effect on performance.. As a result of the statistical values of this study, it was found that players must possess at least 9 out of 17 types of physical abilities Therefore, it can be considered that training and intervention to acquire these physical abilities are essential.

The development of a new type of functional fresh apple juice using prebiotic fibers, ginger extract, and cardamom essential oil: Antioxidant capacity and chemical analysis

  • Hamed Hassanzadeh;Mohammadyar Hosseini;Yaseen Galali;Babak Ghanbarzadeh
    • Food Science and Preservation
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    • v.30 no.5
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    • pp.743-757
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    • 2023
  • The formulation of a novel functional fresh apple juice enriched with dietary prebiotic fiber (inulin or polydextrose), ginger extract (GE), and cardamom essential oil (CEO) was carried out based on a combined D-optimal design. In the first stage, sensory evaluation was performed to screen and select the optimum sample for further experiments. The sensory evaluation showed that the sample containing inulin 0.25 g/100 g GE and 0.03 g/100 g CEO had the highest organoleptic score. In the second stage, various chemical experiments, including pH, acidity, formalin index, total phenol, flavonoids, antioxidant capacity, and vitamin C content, were evaluated on the selected enriched apple juices. The addition of GE and CEO caused changes in nutritional characteristics, including antioxidant capacity, total phenol, flavonoids, vitamin C, and IC50, from 35 g/100 g, 350 mg GAE/g, 17 mg/L, 370 mg/kg, and 1,800 mg/kg to 45 g/100 g, 460 mg GAE/g, 21 mg/L, 420 mg/kg, and 1,200 mg/kg respectively. The steady shear flow and dynamic oscillatory shear rheological tests were also performed on the screened samples, and results showed that the addition of dietary fiber in apple juices increased the apparent viscosity, storage modulus, loss modulus, and complex viscosity. In general, adding plant extracts and processed essential oil to apple juice increased the nutritional-nutraceutical value and sensory attributes of apple juice.

A NOVEL PHOTOHETEROTROPHIC MUTANT FOR psaB GENE OF Synechocystis sp. PCC 6803 GENERATED FROM TARGETED MUTAGENESIS

  • Kim, Soohyun;Kim, Seung-Il;Choi, Jong-Soon;Chung, Young-Ho;Chun, Soon-Bai;Park, Young-Mok
    • Journal of Photoscience
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    • v.3 no.1
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    • pp.23-28
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    • 1996
  • To investigate the structure and function of photosystem I, cartridge mutagenesis technique was used to inactivate the psaB gene of photosystem I. From the screen, many strains which have potential defects in photosystem I were generated. Biochemical analysis revealed that B2, one of the mutant, had a reduced amount of chlorophyll. Electron transfer activitx from photosystem II to photosystem I as oxygen uptake was the rate of 64 % of wild type. Also B2 showed a decreased photosystem I activity when measured by 77 K fluorescence emission spectrum. Particularly, immunodetection analysis showed that the B2 had reduced amount of PsaA/PsaB, but a normal range of PsaC and PsaD. Here we present a photoheterotrophic mutant for psaB gene as a unique model strain for future study of structural/functional relationship and biogenesis of photosystem I.

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Molecular Breeding of Genes, Pathways and Genomes by DNA Shuffing

  • Stemmer, Willem P.C.
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.7 no.3
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    • pp.121-129
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    • 2002
  • Existing methods for optimization of sequences by random mutagenesis generate libraries with a small number of mostly deleterious mutations, resulting in libraries containing a large fraction of non-functional clones that explore only a small part of sequence space. Large numbers of clones need to be screened to find the rare mutants with improvements. Library display formats are useful to screen very large libraries but impose screening limitations that limit the value of this approach for most commercial applications. By contrast, in both classical breeding and in DNA shuffling, natural diversity is permutated by homologous recombination, generating libraries of very high quality, from which improved clones can be identified with a small number of complex screens. Given that this small number of screens can be performed under the conditions of actual use of the product, commercially relevant improvements can be reliably obtained.

A Study on the Design of Prototype for Developing Webpage of Fisheries and Marine Education (수해양 교육을 위한 웹페이지 프로토타입 설계 연구)

  • HEO, Gyun;CHO, Jae-Hyun;HAN, Sang-Jun;WON, Hyo-Heon
    • Journal of Fisheries and Marine Sciences Education
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    • v.27 no.6
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    • pp.1602-1609
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    • 2015
  • The purpose of this study is to design of prototype for developing webpage of fisheries and marine education especially in the field of Busan area. For this, we extract the guideline for development from literature review, need and task analysis, and advices of subject domain experts. Followings were the results of the research: (a) We make the prototype for main menu and screen based on institute, time, subject, and location. (b) We make the prototype for program approach guide, which is consisted of location based menu constructing prototype guide, activation prototype guide, and selection prototype guide. (c) We make the prototype for functional guide, which is consisted of information section, participation section and other sections.

Symbol Characters Allocation of a QWERTY Type Keyboard Design for Smartphones

  • Kim, Kuk
    • Industrial Engineering and Management Systems
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    • v.13 no.4
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    • pp.408-413
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    • 2014
  • The QWERTY type keyboard is a classical device that has been used for computers for a long time. The keyboard design of mobile devices like smartphones is an important issue to consider because of the limited space on the touch screen. This paper presents a design for symbol allocation on the QWERTY type soft keyboard. A 27-cell model, including the full stop (.), is proposed in this paper. A QWERTY type keyboard for smartphones is mainly known for its spatial compatibility, whereas the characters of the ANSI keyboard are allocated to the shoulder positions for functional auxiliary input methods such as the long pressing method.

The Study on the GIS Software Engine based on PDA using GPS/GIS (GPS/GIS를 이용한 PDA기반 GIS 소프트웨어 엔진 연구)

  • PARK, Sung-Seok;KIM, Chang-Soo;SONG, Ha-Joo
    • Journal of Fisheries and Marine Sciences Education
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    • v.17 no.1
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    • pp.76-85
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    • 2005
  • GIS (Geographic Information Systems) technology is a necessary function to support location based on service by using GPS in the mobile environment. These mobile systems have basic functional limitations such as a low rate of processing, limited memory capacity, and small screen size. Because of these limitations, most of the mobile systems require development of a reduced digital map to overcome problems with large-volume spatial data. In this paper, we suggest using the reduced digital map format in order to use location based on service in a PDA environment. The processing of the proposed data format consists of map generation, redefinition of layers, creating polygons, and format conversion. The proposed data format reduces the data size by about 98% comparing with DXF format based on the digital map of Busan.

An Investigation on the Dielectric and Microwave Properties of Ag(Ta,Nb)O3 Thick Films on the Alumina Substrates (알루미나 기판에 스크린 프린팅된 Ag(Ta,Nb)O3 후막의 유전특성 및 초고주파 특성에 대한 연구)

  • Lee, Ku-Tak;Koh, Jung-Hyuk
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.24 no.11
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    • pp.925-928
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    • 2011
  • Perovskite niobates and tantalates have been placed on a short list of functional materials for future technologies. This article was investigated ferroelectric materials $Ag(Ta,Nb)O_3$ thick film. In this study, we have fabricated the $Ag(Ta,Nb)O_3$ thick film on the $Al_2O_3$ substrates by screen printing method. The $Ag(Ta,Nb)O_3$ thick film were fabricated by the mixed oxide method. The sintering temperature and time were 1,150$^{\circ}C$, 2 hr. The electrical properties of $Ag(Ta,Nb)O_3$ thick film were investigated at 30~100$^{\circ}C$.