Molecular Breeding of Genes, Pathways and Genomes by DNA Shuffing |
Stemmer, Willem P.C. (Maxygen, Inc., 515 Galveston Drive, Redwood City) |
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Rapid evolution of a protein invitro by DNA shuffling
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Molecular breeding of viruses
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Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling
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Rapid evolution of a cytokine using molecular breeding
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DNA shuffling of genes from diverse species accelerates directed evolution
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Genome shuffling leads to rapid phenotypic improvement in bacteria
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Novel enzyme activities and functional plasticity revealed by recombining highly homologous enzymes
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8 |
Breeding of retroviruses by DNA shuffling for improved stability and processing yields
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Single-step PCR assembly of a gene and a whole palsmid from large numbers of oligonucleotides
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10 |
Creation of a functionally diverse enzyme library by DNA family shuffling
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Molecular breeding of viruses for targeting and other clinical properties
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12 |
Evolution of an effective fucosidase from a galactosidase by DNA shuffling and screening
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13 |
Searching sequence space
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14 |
Molecular evolution of an arsenate detoxification pathway by DNA shuffling
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15 |
DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution
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16 |
Improved green fluorescent protein by molecular evolution using DNA shuffling
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DOI ScienceOn |