• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2001년도 Proceedings of 2001 International Symposium
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• pp.77-80
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• 2001

A variety of different methods to generate diverse proteins, including random mutagenesis and recombination, are currently available, and most of them accumulate the mutations on the target gene of a protein, whose sequence space remains unchanged. On the other hand, a pool of diverse genes, which is generated by random insertions, deletions, and exchange of the homologous domains with different lengths in the target gene, would present the protein lineages resulting in new fitness landscapes. Here we report a method to generate a pool of protein variants with different sequence spaces by employing green fluorescent protein (GFP) as a model protein. This process, designated functional salvage screen (FSS), comprises the following procedures： a defective GFP template expressing no fluorescence is firstly constructed by genetically disrupting a predetermined region(s) of the protein, and a library of GFP variants is generated from the defective template by incorporating the randomly fragmented genomic DNA from E. coli into the defined region(s) of the target gene, followed by screening of the functionally salvaged, fluorescence-emitting GFPs. Two approaches, sequence-directed and PCR-coupled methods, were attempted to generate the library of GFP variants with new sequences derived from the genomic segments of E. coli. The functionally salvaged GFPs were selected and analyzed in terms of the sequence space and functional property. The results demonstrate that the functional salvage process not only can be a simple and effective method to create protein lineages with new sequence spaces, but also can be useful in elucidating the involvement of a specific region(s) or domain(s) in the structure and function of protein.

• 한국식품위생안전성학회지
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• 제32권2호
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• pp.83-88
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• 2017

본 연구의 목적은 screen printed 탄소계 전극에 기반하여 기능성 식품중에 포함된 소량의 Sildenafil과 Vardenafil(SDF 및 VDF)을 쉽게 검출하기 위한 전류계 면역센서를 개발하고자 하였다. 본 연구에서 개발한 면역센서는 horseradish peroxidase로 labeling 시킨 후 비경쟁적 샌드위치 ELISA 측정법에 의한 원리를 이용하였다. 개발된 센서는 그래핀 산화물 및 키토산(ErGO-CS) 복합체를 단순한 전기화학적 증착에 의해 screen printed 탄소계 전극을 이용하는 원리이며, 감지된 화학물질을 평가하고 센서특성을 최적화하기 위해 전기 화학적 임피던스분광법, 순환 전압 전류법 등을 포함한 일련의 전기화학적 실험방법에 기초하여 완성되었다. 본 연구에서 개발된 센서는 100 pg/mL ~ 300 ng/mL 농도의 SDF 및 VDF에 대하여 직선상의 농도-의존적인 반응을 나타내었다. 또한 최저 검출 한계는 55 pg/mL이었으며, 센서의 민감도는 $1.02{\mu}Ang/mL/cm^2$로 산출되었다. 센서의 성능은 7.1%의 상대 표준편차로 매우 우수한 재현성을 나타내었다. 본 연구에서 개발된 센서의 전략적 가치는 향후 건강기능식품중에 함유된 SDF 및 VDF과 같은 의약품의 미량을 현장에서 쉽게 분석 할 수 있어서 비용-효과적 측면에서 그 가치가 우수하다는 것을 제시한다.

• 컴퓨터교육학회논문지
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• 제5권4호
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• pp.134-145
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• 2002

본 연구는 학습자가 전자교과서에서 문자별로 초점이 다른 텍스트요인을 학습시 내용흐름에 따라 편하게 읽을 수 있게 하기 위한 기능 및 기술적 방안을 제시한다. 본 연구에서 제시한 전자교과서의 텍스트 화면은 학습자가 전자교과서의 텍스트를 접할 때 설정된 값 이상의 데이터가 디스플레이 되면 마우스나 키보드의 작동 없이 학습자별로 원하는 형태로 자동으로 스크롤되어 쉽게 읽을 수 있게 된다. 또한 학습내용이나 개인차에 따라 제시방법이나 속도를 수시로 제어할 수 있다. 자동 텍스트 스크롤은 텍스트 내용구조(특정 및 영역 분류 등), 제시형태(밀도, 사이즈, 폰트 등), 제시방법(스크롤방법, 속도 등), 프로그램 통제유형(램 상주 등)과 화면디자인 적용(가독성 등)등에 따른 데이터 및 화면의 자동 스크롤 방법으로 텍스트 학습에 대한 개인의 능력 및 성향을 반영할 수 있다. 이를 위한 기술적 해결책을 환경설정단계, 스크롤지정단계, 복사단계, 데이터분석단계, 스크롤코딩단계, 중앙포커스코딩단계, 좌우포커스코딩단계, 실행단계로 나누어 제시하였다.

• 한국표면공학회지
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• 제45권1호
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• pp.43-52
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• 2012

Recently, it has been developed for Eco-environment, Super light, Multi-functional nano materials. As needed mobile parts in Smart phone or TV, computer, information communication for high pass signal, multi-function, Magnetic thin film materials have been developed. As last, magnetic powder, sintered and sputtering parts were thick and low purity than electroplating layer, low pass signal and noise were resulted, vibrated TV screen. Because chemical complex temperature was high and ununiform surface layer, it has been very difficult for data pass in High Frequency (GHz) area. Large capacity data pass is used to GHz. Above GHz, signal pass velocity is dependent on Skin Effect of surface layer. If surface layer is thick or ununiform, attachment is poor, low pass signal and cross talk, noise are produced and leaked. It has been reported technical trend of Electrochemically plating and Surface treatment of Metal, Polymer, Ceramic etc. by dispersion/complex for Multi functional nano-magnetic material in this paper.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2017년도 춘계학술대회 논문집
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• pp.87.1-87.1
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• 2017

High functional catalyst to efficiently produce clean and earth-abundant renewable fuels plays a key role in securing energy sustainability and environmental protection of our society. Hydrogen has been considered as one of the most promising energy carrier as represented by focused research works on developing catalysts for the hydrogen evolution reaction (HER) from the water hydrolysis over the last several decades. So far, however, the major catalysts are expensive transition metals. Here using first principles density functional theory (DFT) calculations we screen various pyrites for HER by identifying fundamental descriptor governing the catalytic activity. We enable to capture a strong linearity between experimentally measured exchange current density in HER and calculated adsorption energy of hydrogen atom in the pyrites. The correlation implies that there is an underlying design principle tuning the catalytic activity of HER.

• 한국융합학회논문지
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• 제11권3호
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• pp.245-252
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• 2020

본 연구는 요가, 필라테스, 코어운동 등 체간 안정성 운동프로그램 적용 전후의 만성 요통 환자의 기능적 움직임, 동적 균형능력 및 체간 안정성의 변화를 확인하고, 3가지 체간 안정성 운동의 효과를 비교하고자 하였다. 만성 요통을 가진 성인 43명을 요가 집단 (n=15)과 필라테스 집단 (n=15) 및 코어 운동 집단 (n=13)으로 분류하였고, Functional Movement Screen (FMS)와 Lower Quater Y-Balance Test (YBT-LQ) 및 Trunk Stability Test (TST)를 통해 기능적 움직임, 동적 균형능력 및 체간 안정성을 각각 측정하였다. 이후 8주간 운동을 적용한 후 모든 변인을 재측정하였으며, 운동 적용 전후 차이를 비교 분석하였다. 그 결과, 세 집단 모두 운동 적용 후 FMS, YBT-LQ 및 TST의 측정값이 적용 전보다 유의하게 증가하였으나, 각 운동 적용에 따른 집단 간의 차이는 나타나지 않았다. 이런 결과는 요가, 필라테스 및 코어 운동이 만성 요통 환자의 기능적 움직임, 동적 균형능력 및 체간 안정성을 개선하는 데 도움이 된다는 것을 보여주었다.

• BMB Reports
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• 제40권3호
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• pp.302-314
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• 2007

N type calcium channels (CaV2.2) play a key role in the gating of transmitter release at presynaptic nerve terminals. These channels are generally regarded as parts of a multimolecular complex that can modulate their open probability and ensure their location near the vesicle docking and fusion sites. However, the proteins that comprise this component remain poorly characterized. We have carried out the first open screen of presynaptic CaV2.2 complex members by an antibody-mediated capture of the channel from purified rat brain synaptosome lysate followed by mass spectroscopy. 589 unique peptides resulted in a high confidence match of 104 total proteins and 40 synaptosome proteome proteins. This screen identified several known CaV2.2 interacting proteins including syntaxin 1, VAMP, protein phosphatase 2A, $G_{o\alpha}$, G$\beta$ and spectrin and also a number of novel proteins, including clathrin, adaptin, dynamin, dynein, NSF and actin. The unexpected proteins were classified within a number of functional classes that include exocytosis, endocytosis, cytoplasmic matrix, modulators, chaperones, and cell-signaling molecules and this list was contrasted to previous reports that catalogue the synaptosome proteome. The failure to detect any postsynaptic density proteins suggests that the channel itself does not exhibit stable trans-synaptic attachments. Our results suggest that the channel is anchored to a cytoplasmic matrix related to the previously described particle web.

• 동력기계공학회지
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• 제22권1호
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• pp.64-71
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• 2018

Currently, functional patterns are formed by screen printing on stretchable films, and they are applied to wearable and stretchable devices. In this study, three types of silver paste were prepared using three polyester binders with different Tg and molecular weights in order to impart elasticity to the conductive pattern itself. Rheological properties and DSC measurements were performed for each silver paste. Then, each silver paste was screen printing and cured by an IR dryer to evaluate adhesive strength, pencil hardness, resistance and surface shape change according to strain. As a result, it was found that the silver paste using a binder with a low Tg and a high molecular weight has the smallest resistance change depending on the strain. Namely, it was found that it is most preferable to use a binder with a low Tg and a high molecular weight as the stretchable electrode.

• 대한의용생체공학회:의공학회지
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• 제43권4호
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• pp.219-229
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• 2022

In this study, we propose a miniaturized micro-ceramic heater device. After screen-printing a silver paste between pre-sintered two aluminum oxide plates to integrate a heating circuit, the device was fabricated through a low-temperature sintering process. In order to configure the optimal heating circuit integration condition, the output current evaluation and heating test were performed according to the number of screen prints of the silver paste at various voltages. A silver paste-based heating circuit printed with a line width of 200 ㎛ and a thickness of 60 ㎛ was successfully integrated on a pre-sintered alumina substrate through a low-temperature sintering process. In the case of the 5 times printed device, the thermal response showed a response rate of 18.19 ℃/sec. To demonstrate feasibility of the proposed device in the medical field, such as bio-tissue suturing and hemostasis, a voltage was applied to pig tissue in the device to test tissue change due to heat generated from the device. These results show the possibility that the proposed small ceramic heater could be used in the medical field based on its excellent temperature response.

• 대한통합의학회지
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• 제9권3호
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• pp.111-124
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• 2021

Purpose : The purpose of this study is to evaluate whether physical ability is improved by body stabilization, develop training methods to improve physical fitness factors suitable for body conditions, and provide future management and exercise materials to improve Taekwondo athletes' performance, after conducting the Body stabilization exercise (BSE) program on 22 elementary school Taekwondo athletes for 10 weeks to strengthen core muscles and evaluating Visual response speed test (VRST), Functional movement screen (FMS), Y-Balance test (YBT) and Vital capacity (VC). Methods : In order to examine the impact of the BSE program on Taekwondo athletes' VRST, FMS, YBT and VC this study conducted individual evaluations on all subjects, including VRST, FMS, YBT and VC before applying the programs, and compared exercise effects before and after the program, before and after exercise by a certain period. Results : After exercising, strength and quickness were improved significantly and reaction time was significantly shortened (p<.001). The total score of FMS was improved from 10.86±1.17 before to 11.68±0.99 after exercising, was statistically significant (p=.014). YBT was significantly improved from 91.11±4.84 before to 95.68±5.15 after exercising (p<.001). VC improved was statistically significant (p<.001). Conclusion : In conclusion, the BSE athletic program is expected to help Taekwondo athletes perform well by preventing and reducing injuries through the improvement of muscular strength, strength, speed, agility, step, kick, balance, flexibility, full body endurance and the combination of technical abilities.