• 제목/요약/키워드: Functional compost

검색결과 25건 처리시간 0.017초

Dynamics of Functional Genes and Bacterial Community during Bioremediation of Diesel-Contaminated Soil Amended with Compost

  • Hyoju Yang;Jiho Lee;Kyung-Suk Cho
    • Journal of Microbiology and Biotechnology
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    • 제33권4호
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    • pp.471-484
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    • 2023
  • Compost is widely used as an organic additive to improve the bioremediation of diesel-contaminated soil. In this study, the effects of compost amendment on the remediation performance, functional genes, and bacterial community are evaluated during the bioremediation of diesel-contaminated soils with various ratios of compost (0-20%, w/w). The study reveals that the diesel removal efficiency, soil enzyme (dehydrogenase and urease) activity, soil CH4 oxidation potential, and soil N2O reduction potential have a positive correlation with the compost amendment (p < 0.05). The ratios of denitrifying genes (nosZI, cnorB and qnorB) to 16S rRNA genes each show a positive correlation with compost amendment, whereas the ratio of the CH4-oxidizing gene (pmoA) to the 16S rRNA genes shows a negative correlation. Interestingly, the genera Acidibacter, Blastochloris, Erythrobacter, Hyphomicrobium, Marinobacter, Parvibaculum, Pseudoxanthomonas, and Terrimonas are strongly associated with diesel degradation, and have a strong positive correlation with soil CH4 oxidation potential. Meanwhile, the genera Atopostipes, Bacillus, Halomonas, Oblitimonas, Pusillimonas, Truepera, and Wenahouziangella are found to be strongly associated with soil N2O reduction potential. These results provide useful data for developing technologies that improve diesel removal efficiency while minimizing greenhouse gas emissions in the bioremediation process of diesel-contaminated soil.

Changes in Structural and Functional Responses of Bacterial Communities under Different Levels of Long-Term Compost Application in Paddy Soils

  • Samaddar, Sandipan;Han, Gwang Hyun;Chauhan, Puneet Singh;Chatterjee, Poulami;Jeon, Sunyoung;Sa, Tongmin
    • Journal of Microbiology and Biotechnology
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    • 제29권2호
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    • pp.292-296
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    • 2019
  • Soils amended for long-term with high levels of compost demonstrated greater abundance of bacterial members of the phylum Bacteroidetes whereas a decreasing trend in the relative abundance of phylum Acidobacteria was noted with increasing levels of compost. Metabolic profiles predicted by PICRUSt demonstrated differences in functional responses of the bacterial community according to the treatments. Soils amended with lower compost levels were characterized by abundance of genes encoding enzymes contributing to membrane transport and cell growth whereas genes encoding enzymes related to protein folding and transcription were enriched in soils amended with high levels of compost. Thus, the results of the current study provide extensive evidence of the influence of different compost levels on bacterial diversity and community structure in paddy soils.

Effect of Long Term Fertilization on Microbial Biomass, Enzyme Activities, and Community Structure in Rice Paddy Soil

  • Lee, Chang Hoon;Kang, Seong Soo;Jung, Ki Youl;Kim, Pil Joo
    • 한국토양비료학회지
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    • 제46권6호
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    • pp.487-493
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    • 2013
  • The effects of long-term fertilization on soil biological properties and microbial community structure in the plough layer in a rice paddy soil in southern Korea were investigated in relation to the continuous application of chemical fertilizers (NPK), straw based compost (Compost), combination these two (NPK + Compost) for last 40 years. No fertilization plot (Control) was installed for comparison. Though fertilization significantly improved rice productivity over control, the long-term fertilization of NPK and compost combination was more effective on increasing rice productivity and soil nutrient status than single application of compost or chemical fertilizer. All fertilization treatments had shown significant improvement in soil microbial properties, however, continuous compost fertilization markedly increased soil enzyme and microbial activities as compared to sole chemical fertilization. Results of microbial community structure, evaluated by EL-FAME (ester-linked fatty acid methyl esters) method, revealed big difference among Control, NPK, and Compost. However, both Compost and Compost+NPK treatments belonged to the same cluster after statistical analysis. The combined application of chemical fertilizer and organic amendments could be more rational strategy to improve soil nutrient status and promote soil microbial communities than the single chemical fertilizer or compost application.

기능성 퇴비가 배추의 생육 특성과 수량 및 품질에 미치는 영향 (Effects of Functional Compost on Production, Yield and Quality of Chinese Cabbage)

  • 손상목
    • 한국유기농업학회지
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    • 제10권3호
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    • pp.41-55
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    • 2002
  • Codex유기식품규격의 허용 천연광물자재인 맥반석, 옥, 황토 등을 퇴비에 혼합 첨가하여 제조한 퇴비가 배추생육과 품질에 어떠한 기능성을 가지는가를 검토하고자 Lysimeter에서 재배한 배추의 생육과 수량을 조사하였으며 배추의 당도, 비티민 C 함량 및 질산염 함량 등을 분석하였다. 1. 생체중, 구고, 구폭, 엽신 및 중륵의 무게가 일반 퇴비구에 비해 맥반석, 옥, 황 등을 단독 또는 혼합 처리한 퇴비시용구에서 많았다. 배추 엽수와 엽폭의 증가폭은 그리 크지 않았으나, 엽장은 크게 나타났으며, 맥반석을 혼합하여 처리한 시용구에서 가장 높았다. 2. 당도는 맥반석, 옥, 황토를 혼합 처리한 퇴비시용구에서 퇴비시용구보다 낮았다. 엽신의 비티민 C 함량이 중륵에 비해 훨씬 높았으며, 무비구 578ppm에 비해 기능성퇴비 시용구에서 648~706ppm으로 높아졌고 옥을 혼합하여 처리한 기능성 퇴비 처리구에서 가장 높았다. 3. 질산염 함량은 황토를 혼합 처리한 퇴비시용구에서 엽신 2,021ppm, 중륵 2,235ppm으로 가장 낮아, 황토가 배추의 질산염 집적량을 낮추는데 가장 효과적임을 보여 주었다. 4. 맥반석, 옥, 황토를 단독 혼합하여 제조한 퇴비시용구에 비해 맥반석+옥+황토를 혼합 처리한 퇴비시용구에서 당도, 비티민 C 함량 및 질산염 함량 등은 각각 평균치 수준을 나타냈다. 5. 결론적으로 맥반석, 옥, 황토 등은 배추의 수량증가를 위해 퇴비제조시에 혼합 첨가할 수 있는 기능성 첨가물질로 그 효과가 인정되었다.

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Effect of Long Term Fertilization on Soil Carbon and Nitrogen Pools in Paddy Soil

  • Lee, Chang Hoon;Jung, Ki Youl;Kang, Seong Soo;Kim, Myung Sook;Kim, Yoo Hak;Kim, Pil Joo
    • 한국토양비료학회지
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    • 제46권3호
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    • pp.216-222
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    • 2013
  • Fertilizer management has the potential to promote the storage of carbon and nitrogen in agricultural soils and thus may contribute to crop sustainability and mitigation of global warming. In this study, the effects of fertilizer practices [no fertilizer (Control), chemical fertilizer (NPK), Compost, and chemical fertilizer plus compost] on soil total carbon (TC) and total nitrogen (TN) contents in inner soil profiles of paddy soil at 0-60 cm depth were examined by using long-term field experimental site at $42^{nd}$ years after installation. TC and TN concentrations of the treatments which included N input (NPK, Compost, NPK+Compost) in plow layer (0-15 cm) ranged from 19.0 to 26.4 g $kg^{-1}$ and 2.15 to 2.53 g $kg^{-1}$, respectively. Compared with control treatment, SOC (soil organic C) and TN concentrations were increased by 24.1 and 31.0%, 57.6 and 49.7%, and 72.2 and 54.5% for NPK, Compost, and NPK+Compost, respectively. However, long term fertilization significantly influenced TC concentration and pools to 30 cm depth. TC and TN pools for NPK, Compost, NPK+Compost in 0-30 cm depth ranged from 44.8 to 56.8 Mg $ha^{-1}$ and 5.78 to 6.49 Mg $ha^{-1}$, respectively. TC and TN pools were greater by 10.5 and 21.4%, 30.3 and 29.6%, and 39.9 and 36.3% in N input treatments (NPK, Compost, NPK+Compost) than in control treatment. These resulted from the formation and stability of aggregate in paddy soil with continuous mono rice cultivation. Therefore, fertilization practice could contribute to the storage of C and N in paddy soil, especially, organic amendments with chemical fertilizers may be alternative practices to sequester carbon and nitrogen in agricultural soil.

친환경 잔디관리를 위한 가축분퇴비 중 기능성미생물의 분리 및 선발 (Isolation and Selection of Functional Microbes for Eco-friendly Turfgrass Management in Golf Course from Livestock Manure Compost)

  • 정제용;김영선;조성현;이긍주
    • Weed & Turfgrass Science
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    • 제6권2호
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    • pp.157-164
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    • 2017
  • 본 연구는 미생물을 이용하여 친환경적인 잔디관리를 위해 계분이나 돈분의 퇴비화 과정에서 얻어진 가축분 퇴비로부터 단백질 및 탄수화물 분해능과 잔디 갈색퍼짐병(large patch), 갈색마름병(brown patch), 그리고 동전마름병(dollar spot) 병원균에 항균활성을 보이는 미생물을 분리하기 위하여 실시하였다. 분리된 미생물은 총 68균주였고, 이들을 대상으로 단백질 분해활성, 탄수화물 분해활성 및 잔디 주요병원균에 대한 항균활성을 조사하여 활성이 높은 미생물 34균주를 선발하였다. 이 중에서 단백질과 탄수화물 분해 및 항균활성을 나타내는 균주인 ASC-14, ASC-18 및 ASC-35를 선발하였다. 이들 선발 균주를 대상으로 16s rRNA 유전자 분석 결과 ASC-14와 ASC-18은 B. amyloliquefaciens로 확인되었고, 반면에 ASC-35는 B. subtilis 세균으로 최종 동정되었다.

Long-term Effects of Inorganic Fertilizer and Compost Application on Rice Sustainability in Paddy Soil

  • Lee, Chang Hoon;Park, Chang Young;Jung, Ki Youl;Kang, Seong Soo
    • 한국토양비료학회지
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    • 제46권3호
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    • pp.223-229
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    • 2013
  • Sustainability index was calculated to determine the best management for rice productivity under long-term inorganic fertilizer management's practices. It is based on nutrient index, microbiological index and crop index related to sustainability as soil function. Indicators for calculating sustainability index were selected by the comparison of soil properties and rice response in paddy soil with fertilization. Total twenty two indicators were determined to assess nutrient index, microbiological index and crop index in order to compare the effect of different fertilization. The indices were applied to assess the sustainability with different inorganic fertilizer treatments such as control, N, NK, NP, NPK, NPK+Si, and NPK+Compost. The long-term application of compost with NPK was the highest sustainability index value because it increased nutrient index, microbial index and crop index. The use of chemical fertilizers resulted in poor soil microbial index and crop index, but the treatments like NP, NPK, and NPK+Si were maintained sustainability in paddy soil. These results indicate that application of organic and chemical fertilizer could be a good management to improve rice sustainability in paddy soil.

퇴비로부터 분리된 Burkholderia cepacia No.15-2의 특성과 항균 효과 (Characteristics and Antimicrobial Effects of Novel Burkholderia cepacia No. 15-2 Isolated from Compost)

  • Yun, Soon-Il
    • 한국미생물·생명공학회지
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    • 제31권4호
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    • pp.421-428
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    • 2003
  • 길항미생물을 이용한 항곰팡이성 퇴비의 개발을 목적으로 공시 식물로써 Spinacia oleracea L과 식물 병원균 Rhizoctonia solani Kuhn O-28을 모델로 사용하여 수행 되었다. 다양한 음식쓰레기를 일년동안 숙성시킨 퇴비로부터 80 균주를 분리하였고, 그 중 No.15-2 균주가 R. solani Kuhn O-28에 대해 가장 높은 항곰팡이 활성을 보였다. 16S rDNA sequencing과 primer pair PCR에 의해 표현형과 분류학적으로 거의 독성이 없는 것으로 밝혀진 Burkholderia cepacia genomoval V로 분류되었다. 이 B. cepacia No. 15-2가 퇴비화 중에 우점하였으며 그 균수는 15일 동안 거의 $10^{13}$ cfu/g을 유지하였다. S. oleracea L을 배양 했을 경우 R. solani Kuhn O-28에 의한 발병율은 B. cepacia No.15-2를 첨가함으로써 40% 감소하였다. 결론적으로 B. cepacia No.15-2는 다양한 식물의 곰팡이 유래의 병을 방제하는 데 이용 가능할 것으로 사료된다.

산성 광산 폐수 처리용 생물반응기에 사용되는 유기물의 연구 (Study on Organic Material Used in Bioreactor for the Treatment of Acid Mine Drainage)

  • 김경호;나현준;이성택
    • 한국토양환경학회지
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    • 제2권1호
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    • pp.45-50
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    • 1997
  • 산업구조의 변화와 더불어 많은 수의 휴ㆍ폐광산이 생김에 따라 그에 따른 환경오염 문제가 발생되고 있다. 특히 주로 황철광에서 생성된 폐수의 중금속 이온과 낮은 pH는 생태계를 파괴하는 피해를 입힌다. 따라서 산성광산폐수의 처리를 위한 많은 방법들이 연구되고 개발되고 있다. 본 연구에서는 산성광산 폐수의 생물학적 처리에서 사용되는 4가지 유기물원들의 중금속 처리 능력을 비교 분석하였다. 버섯퇴비, 참나무 퇴비, 슬러지 cake, 우분의 4가지 유기물원 중 산성 광산 폐수의 처리에 효과가 있는 것은 참나무 퇴비와 우분이었다. 참나무 퇴비는 주로 이온 교환이나 -OH와 -COO-등의 작용기에 의한 흡착에 의해서 중금속을 처리하였으며, 우분을 사용한 경우는 자체 내에 존재하고 있는 황산염환원균의 활성에 의해서 중금속을 처리하였다. 따라서 이 두 가지 유기물원을 혼합하여 사용한다면 상호 보완 작용에 의해 효과적인 처리 효율을 얻을 수 있을 것이다.

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