• Journal of Embryo Transfer
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• v.18 no.3
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• pp.243-248
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• 2003

Sperrnatogenesis, the process by which the male germ-line stem cells(GSCs; type A spermatogonia) divide and differentiate to produce the mature spermatozoa, occurs in the seminiferous tubules of the testis. The GSCs proliferate actively to produce two types of cells: other GSCs and differentiating spermatogonia. GSCs have unipotentcy, devoted solely to the generation of sperm. The function of GSCs has broad implications for development, disease, and evolution. Spermatogenesis is fundamental for propagation of species and the defects of this system can result in infertility or disease. The ability to identify, isolate, culture, and alter GSCs will allow powerful new approaches in animal transgenesis and human gene therapy relating to infertility. Until recently, research on stem cells in the testis has been limited because of technical difficulties in isolating and identifying these cell populations. Here, we were trying to find out optimal conditions for in vitro culture of GSCs for identifying and isolating GSCs. We collected mouse GSCs from 3-days old mouse by two-step enzyme digestion method. GSCs were plated and grown on mouse embryonic fibroblasts in Dulbecco's modified Eagle's medium (DMEM) containing 15％ fatal bovine serum, 10 mM 2-mercaptoethanol, 1％ non-essential amino acids, 1 ng/$m\ell$ bFGF, 10 $\mu$M forskolin, 1500 U/$m\ell$ human recombinant leukemia inhibitory factor (LIF). Over a period 3∼5 days, GSCs gave rise to large multicellular colonies resembling those of mouse pluripotent stem cells. After 5th passages, cells within the colonies continued to be alkaline phosphatase and Oct-4 positive and tested positive against a panel of two immunological markers(Integrin $\alpha$ 6 and Integrin $\beta$ 1) that have been recognized generally to characterize GSCs. SSEA-1, SSEA-3, and SSEA-4 also showed positive signals. Based on our data, these GSCs-derived cultures meet the criteria for GSCs itself and even other pluripotent stem cells. We reported here the establishment of in vitro cultures from mouse male GSCs.

• Archives of design research
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• v.19 no.2 s.64
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• pp.63-72
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• 2006

The design in modern industrial society is the core of corporate administrational strategy and a factor that determines the competitiveness of a country. And the process of purchasing products is the matter related to the awareness of design which expresses the desire to own the design product that consumers prefer, implying that the aesthetic feature of product has become a critical characteristics of product design rather than the technical function of product. In this regard, the development of product design with a new dimension is urgently called for, by analyzing and shedding light on the aesthetic features of factors related to the preference of consumers. This study considers the preceding studies on the aesthetic factors and the reaction of customers to the product design to figure out the importance of factors related to the preference of customers, in an effort to shed light on the aesthetic factors that affect the customers' preference of product and the evaluation of product. In addition to that, this study investigated the preference that Korean and Chinese customers display toward the product and assessed the aesthetic features of preferred product in order to come to the conclusion from the experiment and analysis on how much effect the aesthetic factors and the aesthetic factors by product have on the preference of customers. Therefore, this study tries to propose the data that can be useful for the design of product targeted for consumers in Korea and China, by shedding light on what aesthetic factors that products preferred by consumers in Korea and China have and how much effect such factors have on the preference of consumers in an attempt to provide the guideline on the aesthetic factors of the product design.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.2
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• pp.524-531
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• 2017

Summary The purpose of this study was to understand the tendency and the meaning of day care center teachers regarding role playing through metaphorical analysis. The data were collected from 166 day care center teachers who participated in A-city university supplement education using the sentence completion metaphorical method. The collected data were categorized and analyzed through a qualitative research method conducted by 2 early childhood education specialists. The results are as follows. First, the tendency of role playing metaphorical expression was divided into 3 categories, 8 contents and the frequency of 'sociality development' was the highest followed by 'emotional development', 'development'. Second, the meaning of role playing metaphorical expression was recognized as 'social skills', 'role experience', 'imitation', and 'understanding of society' in the 'sociality development' category; as 'imagination', 'purification function', and 'means of expression' in the 'emotional development' category; and as 'essential factor of development' in the 'development' category. Based on the research result, it was suggested that the roles of education and teachers for the value and effective operation of role playing in early childhood education institutes should be reconsidered.

• Journal of KIISE:Databases
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• v.36 no.3
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• pp.198-214
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• 2009

Trajectories of RFID tags can be modeled as a line, denoted by tag interval, captured by an RFID reader and indexed in a three-dimensional domain, with the axes being the tag identifier (TID), the location identifier (LID), and the time (TIME). Distribution of tag intervals in the domain space is an important factor for efficient processing of a query for tracing tags and is changed according to arranging coordinates of each domain. Particularly, the arrangement of LIDs in the domain has an effect on the performance of queries retrieving the traces of tags as times goes by because it provides the location information of tags. Therefore, it is necessary to determine the optimal ordering of LIDs in order to perform queries efficiently for retrieving tag intervals from the index. To do this, we propose LID proximity for reordering previously assigned LIDs to new LIDs and define the LID proximity function for storing tag intervals accessed together closely in index nodes when a query is processed. To determine the sequence of LIDs in the domain, we also propose a reordering scheme of LIDs based on LID proximity. Our experiments show that the proposed reordering scheme considerably improves the performance of Queries for tracing tag locations comparing with the previous method of assigning LIDs.

• Journal of the Korean Institute of Landscape Architecture
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• v.42 no.6
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• pp.111-123
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• 2014

The city square is an important public open space for people. Being used for various activities, such as community gatherings, open markets, concerts, political rallies, and other events, many types of city squares are represented in a city. Nevertheless, most city squares are planned uniformly, lacking consideration for visitor behavior and use satisfaction. The study investigated the design factors and subcomponents influencing user satisfaction with different types of city squares. This study focused on the general city square in Seoul, including the grand public place (i.e. Gwanghwamun Square) and the neighborhood park (i.e. Geumbit Square). The data were analyzed using factor analysis, linear regression and fuzzy theory. The results of the study are as follows: first, five design factors of satisfaction with city squares are identified (Amenity, Usability, Spatial components, Culture, and Comfortableness). Second, Amenity, Comfortableness, and spatial components significantly affect user satisfaction with Gwanghwamun in that order. On the other hand, in Geumbit Square, Comfortableness, Amenity, Usability and Spatial components affect user satisfaction in a significant way, in that order. Third, cleanliness, a subcomponent of amenity, was ranked highest using the fuzzy theory function for satisfaction with Gwanghwamun Square. Otherwise, the prevalence of plants was ranked the highest on the Geumbit Square survey. The study compared design factors influencing satisfaction in the public grand place and the neighborhood park. The results have implications for designing and planning city squares to the satisfaction of their visitors.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.17 no.1
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• pp.225-232
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• 2013

In this paper, we introduce a method that can be used to select the position of head node for context-awareness information. The validity of the head node optimal location is saving the energy in the path according to the clustering. It is important how to elect one of the relay node for energy efficiency routing. Existing LEACH algorithm to elect the head node when the node's energy probability distribution function based on the management of the head node is optional cycle. However, in this case, the distance of the relay node status information including context-awareness parameters does not reflect. These factors are not suitable for the relay node or nodes are included in the probability distribution during the head node selects occurs. In particular, to solve the problems from the LEACH-based hierarchical clustering algorithms, this study defines location with the status context information and the residual energy factor in choosing topology of the structure adjacent nodes. The proposed ECOPS (Energy Conserving Optimal path Schedule) algorithm that contextual information is contributed for head node selection in topology protocols. This proposed algorithm has the head node replacement situations from the candidate head node in the optimal path and efficient energy conservation that is the path of the member nodes. The new head node election technique show improving the entire node lifetime and network in management the network from simulation results.

• Journal of the Korean Institute of Gas
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• v.24 no.4
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• pp.62-72
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• 2020

This study presents a methodology of history matching to evaluate the productivity of shale gas reservoir with high reliability and predict future production rate in the Horn-River basin, Canada. Sensitivity analysis was performed to analyze the effect of physical properties of shale gas reservoir on productivity. Based on the results, reservoir properties were classified into 4 cases and history matching were performed considering the classified 4 cases as objective function. The blind test was conducted using additional field production data for 3 years after the history matching period. The error of gas production rate in Case 1(all reservoir parameters), Case 2(influenced parameters for productivity), Case 3(controllable parameters), and Case 4(uncontrollable parameters) were 7.67%, 7.13%, 17.54%, and 10.04%, respectively. This means that it seems to be effective to consider all reservoir parameters in early period for 4 years but Case 2 which considered influenced parameters for productivity shows the highest reliability in predicting future production. The estimated ultimate recovery (EUR) of production well predicted using the Case 2 model was estimated to be 17.24 Bcf by December 2030 and the recovery factor compared to original gas in place (OGIP) was 32.2%.

• Korean Journal of Remote Sensing
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• v.30 no.5
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• pp.687-701
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• 2014

Sea ice is generally accepted as an important factor to understand the process of earth climate changes and is the basis of earth system models for analysis and prediction of the climate changes. To continuously monitor sea ice changes at kilometer scale, it is demanded to create more accurate grid data from the current, limited sea ice data. In this paper we described a downscaling method for Advanced Microwave Scanning Radiometer 2 (AMSR2) Sea Ice Concentration (SIC) from 10 km to 1 km resolution using a weighting scheme of sea ice days ratio derived from Moderate Resolution Imaging Spectroradiometer (MODIS) sea ice cover product that has a high correlation with the SIC. In a case study for Okhotsk Sea, the sea ice areas of both data (before and after downscaling) were identical, and the monthly means and standard deviations of SIC exhibited almost the same values. Also, Empirical Orthogonal Function (EOF) analyses showed that three kinds of SIC data (ERA-Interim, original AMSR2, and downscaled AMSR2) had very similar principal components for spatial and temporal variations. Our method can apply to downscaling of other continuous variables in the form of ratio such as percentage and can contribute to monitoring small-scale changes of sea ice by providing finer SIC data.

• The Korean Journal of Mycology
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• v.38 no.2
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• pp.160-166
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• 2010

A homothallic filamentous fungus Aspergillus nidulans has been used as the a model organism for studying growth and development for eukaryotic system. Various studies about specific transcription factors have been performed for elucidating the molecular mechanisms of growth, asexual and sexual developmental processes. Among them, the fkhE gene (AN2025.3) is located in chromosome VII and contains an ORF encoding 718 amino acid polypeptide intervening with two short introns. The cDNA sequencing revealed that at least four types of alternative splicing events were occurred when the fkhE gene was transcribed. The putative FkhE polypeptide contains a conserved forkhead domain and a bipartite nuclear localization signal at it's N-terminus and C-terminus, respectively. Deletion of fkhE resulted in impaired conidiophore formation in a solid medium. However, the sexual developmental process or cleistothecia formation was normal. Furthermore, fkhE deletion mutant produced conidiophores and conidia under the submerged culture, indicating that the fkhE gene is involved in asexual developmental process similar to the fkhF gene.

• Journal of Chest Surgery
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• v.31 no.5
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• pp.494-501
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• 1998

From May 1, 1993 to May 31 1995, the authers studied retrospectively 211 patients who underwent cardiovascular operation with cardiopulmonary bypass(CPB). Because we were interested in new development of ARF(prevalence, mortality rate, and main risk factors), we performed a multivariate statistical analysis about data of patients with preoperative serum creatinine values of less than 1.5 mg/dL. Normal renal function before operation(serum creatinine level less than 1.5 mg/dL) was registered in 198(74%) patients. Of these, 27(14%) patients showed postoperative renal complication, including 20(10%) patients classified as renal dysfunction(serum creatinine level between 1.5 and 2.5 mg/dL) and 7(4%) patients as acute renal failure(serum creatinine level higher than 2.5 mg/dL). The mortality rate was 5.8% in normal patients, 5% in patients with renal dysfunction, and 43% when acute renal failure developed(p=0.036). Indeed, the renal impairment proved to be an independent predictor of mortality(odd ratio 2.52∼11.25), along with cardiovascular(odd ratio 4.20) and respiratory(odd ratio 2.18) complications. Multivariate analysis identified the following variables as independent risk factors for postoperative renal impairment : advanced age(odd ratio 1), need for emergency operation(odd ratio 3.78), low-output syndrome(odd ratio 3.66), respiratory complication(odd ratio 1.30), need for deep hypothermic circulatory arrest(odd ratio 1.4). The 13 patients(7%) with preoperative renal failure showed a significantly higher morbidity and mortality rate than those without renal complications before operation. We concluded that the likelihood of severe renal complications is resonably low in the patients undergoing cardiac operation without preexisting renal dysfunction, but associated mortality remains high. A prominant role of hemodynamic factor in the development of postoperative acute renal failure must be recognized during preoperative, intraoperative, and postoperative periods.