• Journal of Korean Society of Forest Science
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• v.99 no.4
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• pp.501-507
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• 2010

Formaldehyde responsive protein(FRP) 1 belongs to the family of universal stress protein(USP) and is known to respond to stress caused by fumigation of gaseous volatile organic compounds(VOCs) such as formaldehyde and toluene. However, the molecular function of this protein is not well understood at cellular and molecular level. In this study, loss of function mutant of FRP1 generated by T-DNA insertion(frp1-4) has been isolated from Arabidopsis thaliana and the function of FRP1 was characterized. The loss-of-function mutant of FRP1 appeared slight growth defects with shorter stem and rosette leaves compared to wild type. In addition, the damage caused by exogenous VOCs was more severe in frp1-4 than in control. Therefore, Arabidopsis FRP1 seems to be the protein involved not only in the growth and development of plant but also the stress resistance against toxic volatile organic compounds.

• Korean Journal of Environmental Agriculture
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• v.6 no.1
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• pp.38-43
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• 1987

This study was carried out to investigate the effect of ozone gas on paddy rice at the different nutrition levels. Jinjubyeo variety of rice plant was exposed to 0.3 ppm ozone gas for 3 hours. It was cultivated at three different application levels, optimum, -50%, and +50% of optimum of nitrogen, phosphorus, and potassium, respectively. After ozone gas fumigation, percentage of damaged leaf, malondialdehyde contents, activity of peroxidase, and nutrient contents of rice plant were observed. The results obtained are as follows. 1) Percentage of damaged leaf was increased at the both additional 50% application of nitrogen and 50% reduction of potassium. 2) Malondialdehyde contents of leaves were increased with the ozone gas exposure. 3) Percentage of damaged leaf was increased at the lower level of $K_2O/N$ ratio in leaves. 4) Nitrogen, phosphorus, and potassium contents in rice leaves were decreased with the ozone gas exposure. 5) The peroxidase bands on gel in electrophoresis were changed by the ozone gas exposure.

• Korean Journal of Agricultural and Forest Meteorology
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• v.2 no.4
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• pp.167-174
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• 2000

A glass chamber experiment was conducted to evaluate the impact of ozone (O$_3$) on the physiology of two soybean, Glycine max L. cultivars, 'Hwanggum' and 'Jangyub'. Thirty-day old plants with 1-2 nodes were exposed to $O_3$ of <10 and 150 n1 1$^{-1}$ in the chambers for 8 h d$^{-1}$ for 3 days. Net photosynthesis, stomatal conductance, chlorophyll a, chlorophyll b and total chlorophyll contents, and foliar injury (% injured leaves) were measured. Although foliar damage was more severe on Jangyub than on Hwanggum, net photosynthesis was decreased by 60% on Hwanggum and 13% on Jangyub due to the $O_3$ treatment. Stomatal conductance on Jangyub was twice higher than that on Hwanggum and it was not changed by the $O_3$ treatment. Whereas, stomatal conductance on Hwanggum was 60-80% decreased by $O_3$, Chlorophyll contents did not change due to the $O_3$ treatment or variety. We can conclude that the reduction of net photosynthesis by $O_3$ was mainly due to the decreases of stomatal conductance and Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) activity on dark reaction. And foliar injury and chlorophyll content did not contribute to the net photosynthetic decrease. The gas-exchange variables measured 24 h after the termination of $O_3$ fumigation showed that there was no significant recovery within a day. Since the physiological responses on Jangyub were not much affected by the $O_3$ treatment, this variety could be $O_3$ resistant.

• 보존과학연구
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• s.32
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• pp.123-136
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• 2011

There are more than 2,800 different species of termites in the world, but just there is one species (Reticulitermes speratus kyushuensis Morimoto) in Korea. Once wooden structures are damaged by termites, we are applying chemical control methods such as fumigation, treatment of insecticidal and antiseptic chemicals, soil termiticide treatment, and termite colony elimination system to pest control. But in Korea, the termite infestation is gradually increasing, so it is essential to study on the present situation of termite control in US or Japan. Accordingly, in this part we have studied focusing on the case of US. In the US, there are three groups of termites : Subterranean, Drywood, Dampwood termites, and they caused more severe infestation compared with Korea. When a structure has become infested with termites, it is important that appropriate action must be taken: the chemical pest control (soil treatment, termite baiting, termite colony elimination), the modification and maintenance of buildings and the regular inspection for follow-up. And with consideration for different characteristics of termites, the process of pest control is made according to each species of termites. Most of the subterranean termite control is done by applying either termiticide to the soil or termite monitoring and baiting system around the structure. On the other hand, drywood termite control methods can be categorized as either the treatment for the whole structure or the treatment for localized area. Applications to the whole structure are done by fumigants or heat and the localized treatments are carried out with chemicals as well as heat, freezing, microwave and electricity.

• Horticultural Science & Technology
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• v.28 no.3
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• pp.394-402
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• 2010

The objective of this study was to identify the effects of ozone on the two species in controlled environment. $Sedum$ $kamtschaticum$ and $Hosta$ $longipes$ were exposed in both control and ozone chamber to investigate photosynthesis, antioxidant enzyme activity, visible damage, the number and the size of stomata and the plastogloubuli. Ozone was fumigated in the concentration of $200{\mu}g{\cdot}kg^{-1}$ for 8 hours in a day (from 08:00 AM to 04:00 PM). Firstly, net photosynthesis of two species was decreased after ozone fumigation. Secondly, glutathione reductase activities showed significant difference between control and ozone treatment. Thirdly, visible symptoms of leaves were expressed such as chlorosis, necrosis and decoloration. Also, the size of stoma was significantly decreased in ozone-exposed plants. Furthermore, the intercellular space of $Hosta$ $longipes$ showed increased phenomenon because the mesophyll was collapsed. The number and the size of the plastogloubuli were significantly larger in ozone stress.

• The Journal of Korean Obstetrics and Gynecology
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• v.28 no.3
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• pp.21-29
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• 2015

Objectives This study aimed to investigate therapeutic effect of Korean Medicine Steam Therapy (KMST) for leukorrhea patients. Methods From December 1st 2013 to Nov 31st 2014, 16 leukorrhea outpatients treated with KMST filled out questionnaire before and after treatments. The questionnaire was composed of 8 question items such as sleep, appetite & digestion, urination, defecation, emotion, cold hypersensitivity, menstrual pain, leukorrhea and patients were told to evaluate their symptoms as NRS score. We analyzed mean NRS score of each question item before and after KMST by using Wilcoxon's signed rank test of SPSS ver. 19. Results Mean age of participants was 40.8±13.0 years and mean value of treatment numbers per person was 3.8±1.8 times. 43.75% (n=7) of the patients had history of vaginitis caused by gardnella vaginalis, ureaplasma urealyticum or candida albicans. All patients were treated with acupuncture, moxibustion and Korean medicine. In addition, there was no side effect reported by participants. NRS score of quantity, odor of leukorrhea, perineal unpleasant sensation such as itching, dryness, burning sensation remarkably decreased (p<0.01, p<0.05, p<0.01 each). NRS score of symptoms including sleep, urination, cold hypersensitivity, menstrual pain also remarkably decreased (p<0.05). Conclusions Symptoms of leukorrhea patients including sleep, urination, cold hypersensitivity, menstrual pain, leukorrhea showed significant decrease after treated with concurrent KMST.

• Korean Journal of Soil Science and Fertilizer
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• v.20 no.2
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• pp.139-145
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• 1987

This study was carried out to find out the effects of ammonia gas on the leaf destruction of vegetable crops, and nitrogen contents in their leaves. Radish, chinese cabbage, tomato and cucumber were fumigated with 0.05, 0.1, $0.2mg/{\ell}$ ammonia gas for 2 hrs under different exposure time, light and soil water condition at vegetative growth stage. The results obtained were as follows. The crop damages measured by percentage of leaf destruction were more severe at the night time fumigation than treatment during the day, at the full sun condition than the shading, and at the higher soil water contents. Cucumber plant showed higher resistance to ammonia gas than tomato plant. The chrolophyll contents in leaves of each crops were remarkably decreased by increment of ammonia concentration from $0.05mg/{\ell}$ to $0.2mg/{\ell}$, but the N content in them was increased.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.91-91
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• 2019

전북 완주군 농가에서 2017년 12월에 재배한 '시베리아'와 '메두사' 품종을 1시간 이내에 국립원예특작과학원 실험실로 이동하였다. 알약(SmartFreshTM, AgroFresh Inc., USA)형태의 1-MCP를 1.875g/3.55m3 기준으로 정량하여 Activator Kit(SmartFreshTM, AgroFresh Inc., USA)에 넣어 마개를 닫고 몇 번 흔들어주어 녹인 후 1.5 ppm이 되도록 처리하였으며 3시간 훈증 후 30분 환기하였다. 훈증처리 후 절화 백합은 유공 필름 슬리브를 이용하여 포장한 후 골판지상자에 넣어 모의 수출환경에서 수송방식은 건식(건조처리)상태로 $4^{\circ}C$ 저장고에 암상태로 저장하면서 무처리를 대조로 에틸렌 노출 조건 조성을 위하여 후레쉬라이프(탑프레쉬) 5g 봉지를 상자내부 5개, 상자외부 저장고 내에 10개로 총 15개의 에틸렌 발생제 처리를 하였다. 백합 절화 신선도 유지기간 연장을 위한 선도유지제 전처리 효과는 1.5 ppm 1-MCP 3시간 훈증처리에 의해 잎의 황화현상을 지연시키는 효과가 있었다. 전체적으로 판정한 절화수명은 '시베리아'는 무처리 9.0일, 1-MCP 훈증 9.0일, 에틸렌 발생제 8.3일, 1-MCP 훈증+에틸렌 발생제 9.5일이었으며 '메두사'는 무처리 6.5일, 1-MCP 훈증 7.5일, 에틸렌 발생제 5.7일, 1-MCP 훈증+에틸렌 발생제 8.5일로 나타났다. 에틸렌에 노출은 꽃의 빠른 노화를 야기하여 절화수명을 단축 시켰으며 이상개화를 보이는 꽃도 있어 상품성이 크게 떨어졌다. 1-MCP에 의해 잎의 황화 억제에 효과적이었으며 저장 중 에틸렌 발생제를 동시에 처리하였을 때 품질유지 효과가 더 크게 나타났으며 '메두사' 품종에서는 만개시 화색도 더 진하게 나타났다. 따라서, 저온저장이나 선적시 에틸렌 발생이 많은 품목과 혼합하게 될 것이 예상될 경우에 품질유지를 위해 1-MCP 훈증처리를 하면 효과적일 것으로 사료된다.

• Journal of Ginseng Culture
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• v.6
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• pp.51-79
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• 2024

In the 1970s and 1980s, during the nascent phase of ginseng disease research, efforts concentrated on isolating and identifying pathogens. Subsequently, their physiological ecology and pathogenesis characteristics were scrutinized. This led to the establishment of a comprehensive control approach for safeguarding major aerial part diseases like Alternaria blight, anthracnose, and Phytophthora blight, along with underground part diseases such as Rhizoctonia seedling damping-off, Pythium seedling damping-off, and Sclerotinia white rot. In the 1980s, the sunshade was changed from traditional rice straw to polyethylene (PE) net. From 1987 to 1989, focused research aimed at enhancing disease control methods. Notably, the introduction of a four-layer woven P.E. light-shading net minimized rainwater leakage, curbing Alternaria blight occurrence. Since 1990, identification of the bacterial soft stem rot pathogen facilitated the establishment of a flower stem removal method to mitigate outbreaks. Concurrently, efforts were directed towards identifying root rot pathogens causing continuous crop failure, employing soil fumigation and filling methods for sustainable crop land use. In 2000, adapting to rapid climate changes became imperative, prompting modifications and supplements to control methods. New approaches were devised, including a crop protection agent method for Alternaria stem blight triggered by excessive rainfall during sprouting and a control method for gray mold disease. A comprehensive plan to enhance control methods for Rhizoctonia seedling damping-off and Rhizoctonia damping-off was also devised. Over the past 50 years, the initial emphasis was on understanding the causes and control of ginseng diseases, followed by refining established control methods. Drawing on these findings, future ginseng cultivation and disease control methods should be innovatively developed to proactively address evolving factors such as climate fluctuations, diminishing cultivation areas, escalating labor costs, and heightened consumer safety awareness.

• Applied Biological Chemistry
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• v.16 no.3
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• pp.146-165
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• 1973

Crop damages caused by sulfur dioxide poisoning were studied with respect to physiology of lesion, yield loss and prevention measures. The results are summarized as follows; 1. On the physiology of injury: The sulfur dioxide gas did no: affect the pH and $E_h$ values of the tested leaf juice of plants. Peroxidase activity was inhibited just after sulfur dioxide treatment but gradually recovered to normal after 10 hours. Methanolic chlorophyll solution was instantaneously and irreversibly bleached by the addition of sulfur dioxide gas with no evidence of pheophytin formation. It seems that chlorophyll forms colourless addition product or is reduced to colourless form with either sulfur dioxide gas or sulfurous acid. Chlorophyll in the chloroplast was also bleached by the sulfur dioxide treatment, as in the case of methanolic solution of chlorophyll, except that the rate of bleaching was rather slow, requiring 1-2 hours. It appears that the most inflicting cause of sulfur dioxide gas to plants may be the destruction of chlorophyll by the poisoning gas. 2. On the effects to crop yield: The crop yield losses were proportional to the concentration of inflicting sulfur dioxide gas. The order of tolerence of the crops to the sulfur dioxide gas was as follows - chinese cabbage being the most susceptible; wheat, paddy rice, barley, soybean, welsh onion, radish and chinese cabbage. The crucifer crops were generally found more susceptible than other crops studied. With respect to the growing stages of crops exposed to sulfur dioxide gas, it was found that the flowering stage was the most susceptible fellowed by panicle forming, milky and tillering in the decreasing order of susceptibility. 3. On the preventive measures of yield losses: Soil applications of potassium, wollastonite, lime or spray of lime water were effective to prevent yield losses from sulfur dioxide fumigation of paddy rice, barley, and soybeans. The most responsive treatment was lime water spray for all crops tested. In case of sulfur dioxide fumigated paddy rice, the lime water spray also increased carbon assimilation.