• Korean journal of applied entomology
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• v.42 no.3
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• pp.249-255
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• 2003

These studies were carried out to investigate fumigant, contact toxicity and repellency effects of 34 terpenes against acaricide susceptible the two-spotted spider mite, Tetranychus urticae. In addition, the efficacy was also tested against two acaricide-resistant strains. Two terpenes, isosafrole and safrole showed fumigant toxicity of 98.4%, at 10${\mu}\ell$/1 (air) concentration. LD$\_$50/ values of these two terpenes were 2.6${\mu}\ell$/1 and 4.3${\mu}\ell$l/1, respectively. Most terpenes showed low or no contact toxicity, except isosafrole showing 60.2% mortality against eggs. Hexanoic acid and limonene showed repellency effects of 79.1%, 87.8%, respectively, to the susceptible strain at concentration of 1,000 ppm in the lab conditions. Hexanoic acid (1,000 ppm) showed repellency effected of 77.8% and 83.3% to fenpropathrin and pyridaben resistant strains, respectively. However, limonene showed no repellency to the two resistant strains.

• Korean journal of applied entomology
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• v.41 no.4
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• pp.285-292
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• 2002

These studies were carried out to investigate the fumigant toxicity, the contact toxicity and the ovipositional repellency of 25 monoterpenoids against oak longicorn beetle adults, Moechotypa diphysis. Monoterpenoids with 100% of fumigant toxicity were 1, 8-cinehone, fenchone, pulegone and (equation omitted)-terpinene at 20 (equation omitted)/954 ml (air) concentration, pulegone and (equation omitted)-terpinen at 10 (equation omitted)/954 ml (air) concentration, pulegone at 5 (equation omitted)/954 ml (air) concentration. Most monoterpenoids showed low or no contact toxicity but only pulegone showed about 70% mortality. Some monoterpenoids which had repellency to female adults with Y-tube olfactometer were bornylacetate, carvacrol, 1, 8-cineole and menthol at 1 (equation omitted) dose, while citronellol showed attractant response. Carveol, geraniol and perillyl alcohol of 25 monoterpenoids showed ovipoistional repellency of 82.1%, 78.3%, 87.5%, respectively, at the concentration of 1,000 ppm in the laboratory condition. In the field, the result tested with three monoterpenoids indicated that geraniol was the most effective one at the concentration of 10,000ppm and 1,000 ppm, but residual effect wasn't found.

• Journal of agricultural medicine and community health
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• v.41 no.2
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• pp.63-74
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• 2016

Objectives: We surveyed the awareness and current status of using fumigant carbon disulfide for exterminate Curculio sikkimensis among chestnut farmers in Chungnam Province to suggest directions for health education and public relations. Methods: We designed questionnaires to evaluate recognition of fumigant carbon disulfide. We conducted a questionnaire survey to assess recognition and recognition level of fumigant carbon disulfide by the study variables. Results: The recognition status for fumigant carbon disulfide was 74.5%, but the recognition level was low (know well 27.5%). The path of recognition was 45.1% and 15.7% for neighbor and rural technology center, respectively. The recognition status for warning label of fumigant carbon disulfide was 52.9%. Recognition for warning label of fumigant carbon disulfide was tended to increase with high educational attainment, bigger owning land area. Recognition on the content of warning label were 29.4%, 27.5%, 21.6%, and 21.6% for inflammability, toxicity, hazard, and explosiveness, respectively. Using personal protection equipment was tended to increase with the high status of awareness on fumigant carbon disulfide. Conclusions: Health education programs for using fumigant carbon disulfide are needed for chestnut farmers. In addition, publicity information activities about prevention and protection of carbon disulfide poisoning are needed for high risk farmers.

• The Korean Journal of Pesticide Science
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• v.12 no.3
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• pp.302-306
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• 2008

This study was performed to investigate the contact toxicity, fumigant toxicity, and synergistic effect using agricultural materials (AM) when mixed with selected essential oils against oak longicorn beetle, Moechotypa diphysis. Four kinds of AM, namely, chungkiller, ungsalta, jinap, purunjunsa showed 100% contact toxicity at one day after treatment. Subsequently, four AM was tested the fumigant toxicity during 24 hours. At concentration of 1 mL, jinap and chungkiller showed 100% mortality at 3 hr after treatment, prunjunsa and ungsalta showed 100% mortality at 6 hr after treatment. When applicate to the small vinyl bag (15 L), all AM (50 mL/15 L) was showed 100% mortality. Jinap showed the mortality 100% when mixed pennyroyal oil with the ratio of 5+1. It was higher than single treatment.

• Korean journal of applied entomology
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• v.45 no.1 s.142
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• pp.45-49
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• 2006

Fumigant toxicity of four plant essential oils (muguet flower, patchouli, pennyroyal, spearmint) were tested against the adults of western flower thrips, Frankliniella occidentalis. Among them, pennyroyal and spearmint oils showed 100% mortality at $1{\mu}{\ell}/45m{\ell}$ air dose. Through the constituent analysis using GC and GC-MS, we confirmed that main constituents of pennyroyal oil were pulegone(100.0%), and spearmint oil were $\beta-myrcene(3.5%)$, limonene(12.1%), carvone(85.4%). Among them, carvone and pulegone showed 100% fumigation at $1{\mu}{\ell}/45m{\ell}$ air dose, respectively. It can concluded that two oils are potential control agents against F. occidentalis.

• Korean journal of applied entomology
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• v.45 no.2 s.143
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• pp.189-194
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• 2006

Fumigant toxicity of 18 plant essential oils were tested against the adults of oak longicorn beetle, Moechotypa diphysis. Among them, eucalyptus, rosemary and pennyroyal oils showed 100% mortality and sage oil showed 85% mortality at 10 $\mu\ell/\ell$ (air) dose. Eucalyptus and rosemary oils showed 100% mortality within 6 hr after treatment at 10 $\mu\ell/\ell$ (air) dose. GC and GC/MS analysis of the four essential oils and bioassay of their components revealed that 1,8-cineole (a major component of eucalyptus, rosemary and sage oils), thujone (a major component of sage oil) and pulegone (a major component of pennyroyal oil) showed higher adulticidal activity than others.

• Korean journal of applied entomology
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• v.51 no.4
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• pp.389-395
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• 2012

Stored grain insects appear frequently as grains are harvested, stored, and distributed on the market. Producers mostly depend on chemical fumigants to control and repel insect pests, but this application of chemicals may cause side effects for humans and the environment. Safer control approaches, including the use of natural products are required, and in this study, we assessed the control effect of essential oils on stored grain insects. The essential oils of 8 plant species from 6 families were tested for their fumigant toxicity and repellent effects using a Y-tube Olfactometer against Sitophilus zeamais adults. Pennyroyal and tea tree oil respectively exhibited 97% and 63% mortality at $10{\mu}{\ell}L^{-1}$ air concentration in tested fumigant toxicity. Of particular note, pennyroyal oil ($LT_{50}$, hour=3.87) showed higher adulticidal activities than tea tree oil ($LT_{50}$, hour=4.09). When assessing repellent effects, using a Y-tube Olfactometer at $10{\mu}{\ell}$/filter paper concentration, the cedarwood, cinnamon, cypress, patchouli and pennyroyal essential oils showed potent repellent activities against Sitophilus zeamais adults. Cinnamon, cypress and pennyroyal essential oils showed notably high repellent activities of 82.4%, 80.0% and 86.7%, respectively, at $1{\mu}{\ell}$/filter paper concentration.

• Korean Journal of Veterinary Service
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• v.39 no.2
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• pp.117-124
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• 2016

In this study, the virucidal efficacy of a fumigant containing 20% ortho-phenylphenol against classical swine fever virus (CSFV) and porcine reproductive and respiratory syndrome virus (PRRSV) was examined. After each carrier deposited with CSFV and PRRSV suspensions was exposed to the fumigant in a $25-m^3$ test room for 15 h, all carriers were neutralized and diluted, and each diluted suspension was inoculated into each proper cell line. After incubation, CSFV and PRRSV viability in each cell line was examined and 50% tissue culture infectious dose $(TCID_{50})/mL$ was calculated. In the results, the concentration of viable virus in all of pathogen control-carriers was more than $2{\times}10^5TCID_{50}/mL$, and there were no cytotoxicity in all of toxicity control-carriers. In addition, the fumigant inactivated ${\geq}4.8{\log}_{10}(TCID_{50}/mL)$ of both CSFV and PRRSV. These findings will be useful for preventing the spread of CSFV and PRRSV infection.

• The Korean Journal of Pesticide Science
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• v.10 no.2
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• pp.117-123
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• 2006

Fumigant toxicity of 34 plant essential oils were tested against female adults of Culex pipiens pallens. Seven oils (basil, coriander, fennel, pennyroyal, peppermint, thyme red, thyme white) showed more than 60% mortality at 1 ${\mu}L\;L^{-1}$ air concentration and pennyroyal oil appeared to have the highest adulticidal activity. GC and GC-MS analysis of the seven essential oils and bioassay of their components revealed that pulegone (a major component of pennyroyal oil) and anethole (a major component of fennel and basil oil, $LD_{50}$, ${\mu}L\;L^{-1}$ air =0.49) showed higher adulticidal activities than others. In addition to the above two components, linalool ($LT_{50}$, min=0.91), camphor ($LT_{50}$, min=1.06), fenchone ($LT_{50}$, min=1.16), 1,8-cineole ($LT_{50}$, min=2.93), and geranyl acetate ($LT_{50}$, min=1.60) also showed higher adulticidal activity than empenthrin ($LT_{50}$, min=3.45).

• Toxicological Research
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• v.33 no.4
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• pp.333-342
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• 2017

Ethyl formate, a volatile solvent, has insecticidal and fungicidal properties and is suggested as a potential fumigant for stored crop and fruit. Its primary contact route is through the respiratory tract; however, reliable repeated toxicological studies focusing on the inhalation route have not been published to date. Therefore, the present study was conducted to investigate the safety of a 90-day repeated inhalation exposure in rats. Forty male and 40 female rats were exposed to ethyl formate vapor via inhalation at concentrations of 0, 66, 330, and 1,320 ppm for 6 hr/day, 5 days a week for 13 weeks. Clinical signs, body weights, food consumption, urinalysis, hematologic parameters, serum chemistry measurements, organ weights, necropsy, and histopathological findings were compared between the control and ethyl formate-exposed groups. Locomotor activity decreased during exposure and recovered afterward in male and female rats exposed to 1,320 ppm ethyl formate. Body weight and food consumption continuously decreased in both sexes exposed to 1,320 ppm ethyl formate from week 1 or 3 compared with the control values. The increases in adrenal weight and decreases in thymus weight were noted in both sexes exposed to ethyl formate at 1,320 ppm. Degeneration, squamous metaplasia of olfactory epithelium in the nasopharyngeal tissue, or both were noted in the male and female rats at 1,320 ppm and female rats at 330 ppm ethyl formate. Taken together, our results indicate that ethyl formate-induced changes were not observed in male and female rats at 330 and 66 ppm, respectively. This indicates that exposure to ethyl formate at concentrations below 66 ppm for 90 days is relatively safe in rats. This is the first report of a full-scale repeated inhalation toxicity assessment in rats and could contribute to controlling occupational environmental hazards related to ethyl formate.