• 한국생산제조학회지
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• 제7권1호
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• pp.22-33
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• 1998

A continuum analysis of the evolution of plasticity and Bauschinger effect in a short fiber reinforced metal matrix composite, based on the FEM solution for a single fiber model has been performed to investigate the strengthening behavior. The evolution of matrix field quantities during one cycle of fully reversed loading have been examined in detail. The results indicate that the role of constrained matrix flow in generating different levels of matrix triaxiality during forward and reversed loading provides an important contribution to the developement of the Bauschinger effect in the metal matrix composite. Therefore, even when the plastic flow of the matrix material follows on isotropic hardening behavior, the Bauschinger effect is predicted for the composite material.

• 대한수의학회지
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• 제39권4호
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• pp.806-810
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• 1999

A multiplex-polymerase chain reaction (PCR) assay was used to detect staphylococcal enterotoxin production by 12 strains of Staphylococcus aureus isolated from clinical specimens. To evaluate the efficacy and/or sensitivity of this method, the results were compared to those obtained with the reversed passive latex agglutination kit (SET-RPLA, Denka Seiken, Japan). Of 10 strains positive by PCR were positive by RPLA but two strains, representing high sensitivity of the former method. Enterotoxin B was the most prevalent by the two methods. The kappa index between the two methods was 0.826, indicating a higher agreement and fully reliable for use. These results would suggest that sensitive, inexpensive, and relatively rapid multiplex-PCR technique may be an effective means for the detection of staphylococcal enterotoxin genes as an alternative to traditional methods such as kits or immunological methods, which depend upon the amount of enterotoxin produced.

• 대한수의학회지
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• 제38권3호
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• pp.508-517
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• 1998

There is evidence that the sympathetic nervous system exerts a control on thyroid function via an adrenergic innervation of thyroid cells. Although it is clear that the inhibitory effects of catecholamines result from an activation of ${\alpha}_1$-adrenoceptors, the mechanisms involved in ${\alpha}_1$-stimulation are not fully understood. The effects of methoxamine and protein kinase C (PKC) activator on the release of thyroxine ($T_4$) from mouse thyroid were studied to clarify the role of PKC in the regulation of $T_4$ release in vitro. The glands were incubated in the medium, samples of the medium were assayed for $T_4$ by EIA kits. Methoxamine inhibited the TSH-stimulated $T_4$ release. This inhibition was reversed by prazosin, an ${\alpha}_1$-adrenergic antagonist. Futhermore, the inhibitory effect of methoxamine on the $T_4$ release stimulated by TSH was prevented by chloroethylclonidine, an ${\alpha}_{1b}$-adrenoceptor antagonist, but not by WB4101, an ${\alpha}_{1a}$-adrenoceptor antagonist. Also methoxamine inhibited the forskolin-, cAMP- or IBMX-stimulated $T_4$ release. These inhibition were reversed by PKC inhibitors, such as staurosporine and $H_7$. PMA, a PKC activator, completely inhibited the TSH-stimulated $T_4$ release, and its inhibition was reversed by staurosporine and $H_7$, but not by chelerythrine. R59022 (a diacylglycerol kinase inhibitor), like methoxamine, also inhibited the TSH-stimulated $T_4$ release, and its inhibition was also reversed by staurosporine. The present study suggests that methoxamine inhibition of $T_4$ release from mouse thyroid can be induced by activation of the ${\alpha}_{1b}$-adrenoceptors and that it is mediated through the ${\alpha}_1$-adrenoceptor-stimulated PKC formation.

• 한국분말야금학회:학술대회논문집
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• 한국분말야금학회 2006년도 Extended Abstracts of 2006 POWDER METALLURGY World Congress Part 1
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• pp.140-141
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• 2006

Most PM components are exposed to cyclic loading over long periods of time, yet, the fatigue performance is often at best characterized by a fully reversed bending strength. The effects of density, deviating loading modes, external notches or mean stresses must usually be estimated. The amount of available data is nowadays sufficient to come to fact-based estimates.

• 대한기계학회논문집
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• 제13권2호
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• pp.307-315
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• 1989

본 연구에서는 단조과정을 거친후 노멀라이징(normalizing) 처리된 SF45A강종과 압연후 자연 냉각시킨 SM41B강종의 두 국산 강종에 대해 저사이클 피로시험을 행하여 저사이클 피로특성을 밝히고, 강재의 가공공정과 부하변형율변화에 따른 피로과정중의 연화 및 경화 거동을 관찰하였다. 그리고 저사이클 피로파면의 미시적 고찰을 통해서 부하변형율 크기에 따른 미시적 파면 양상의 변화를 밝혀 파괴 해석의 기초자료로 제공하였다.

• FOCUS: LIFE SCIENCE
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• 제1호
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• pp.3.1-3.7
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• 2024

The article discusses the critical role of chromatography in the analysis and purification of proteins in biopharmaceuticals, emphasizing the importance of comprehensive characterization for ensuring their safety and efficacy. It highlights the use of Avantor® ACE® HPLC columns for the separation and purification of proteins, focusing on the analysis of intact proteins using reversed-phase liquid chromatography (RPLC) with fully porous particles. This article also details the application of different mobile phase additives, such as TFA and formic acid, and emphasizes the advantages of using type B ultra-pure silica-based columns for efficiency and peak shape in biomolecule analysis. Additionally, it addresses the challenges of analyzing intact proteins due to slow molecular diffusion and introduces the concept of solid-core (or superficially porous) particles, emphasizing their benefits over traditional porous particles for the analysis of therapeutic proteins. Furthermore, it discusses the development of Avantor® ACE® UltraCore BIO columns, specifically designed for the high-efficiency separation of large biomolecules, such as proteins, and demonstrates their effectiveness in achieving high-resolution separations, even for higher molecular weight proteins like monoclonal antibodies (mAbs). In addition, it underscores the complexity of analyzing and characterizing intact protein biopharmaceuticals, requiring a range of analytical techniques and the use of wide-pore stationary phases, operated at elevated temperatures and with relatively shallow gradients. It highlights the comprehensive range of options offered by Avantor® ACE® wide pore columns, including both fully porous and solid-core particles, bonded with a variety of complementary stationary phase chemistries to optimize selectivity during method development. The use of ultrapure and highly inert base silica is emphasized for enabling the use of lower concentrations of mobile phase modifiers without compromising analyte peak shape, particularly beneficial for LC-MS applications. Then the article concludes by emphasizing the significance of reversed-phase liquid chromatography and its compatibility with mass spectrometry as a valuable tool for the separation and analysis of intact proteins and their closely related variants in biopharmaceuticals.

• Structural Engineering and Mechanics
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• 제41권6호
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• pp.691-710
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• 2012

One of the most popular and commonly used strengthening techniques to protect against earthquakes is to infill the holes in reinforced concrete (RC) frames with fully reinforced concrete infills. In some cases, windows and door openings are left inside infill walls for architectural or functional reasons during the strengthening of reinforced concrete-framed buildings. However, the seismic performance of multistory, multibay, reinforced concrete frames that are strengthened by reinforced concrete wing walls is not well known. The main purpose of this study is to investigate the experimental behavior of vulnerable multistory, multibay, reinforced concrete frames that were strengthened by introducing wing walls under a lateral load. For this purpose, three 2-story, 2-bay, 1/3-scale test specimens were constructed and tested under reversed cyclic lateral loading. The total shear wall (including the column and wing walls) length and the location of the bent beam bars were the main parameters of the experimental study. According to the test results, the addition of wing walls to reinforced concrete frames provided significantly higher ultimate lateral load strength and higher initial stiffness than the bare frames did. While the total shear wall length was increased, the lateral load carrying capacity and stiffness increased significantly.

• Archives of Pharmacal Research
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• 제27권4호
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• pp.460-464
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• 2004

The quality of Alismatis Rhizoma was evaluated by reversed-phase high performance liquid chromatographic method. Alisol B 23-acetate was used as a standard marker for evaluation. This component was fully separated from the other components in the plant extracts on a ODS column. Identifcation of alisol B 23-acetate was carried out by comparing the LC/MS spectrum of separated peak from the extract with that of standard. Alisol B 23-acetate contents in Alismatis Rhizoma obtained from several herbal markets were varied from $0. 15\%$ to $0.56\%$.

• 한국가시화정보학회:학술대회논문집
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• 한국가시화정보학회 2005년도 추계학술대회 논문집
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• pp.50-53
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• 2005

The turbulent flow around a sphere was investigated in a streamwise meridian plane using two experimental techniques: smoke-wire flow visualization in wind tunnel at Re=5,300 and PIV measurements in a circulating water channel at Re=7,400. The smoke-wire visualization shows flow separation points near an azimuthal angle of $90^{\circ}$, recirculating flow, transition from laminar to turbulent shear layer, evolving vortex roll-up and fully turbulent eddies in the sphere wake. In addition, the mean flow pattern extracted by particle tracing method in water tunnel at Re= 14,500 reveals two distinct comparable toroidal(not closed) vortices in the recirculation region. The mean velocity field measured using a PIV technique demonstrates the detailed wake configuration of close symmetric recirculation and near-wake configuration with two toroidal vortices, reversed velocity zone and vorticity contours.

• International Journal of Precision Engineering and Manufacturing
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• 제3권4호
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• pp.72-77
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• 2002

The load interaction effect can be best illustrated by the phenomenon of overload retardation. Some prediction methods for retardation are reviewed and the problems discussed in the present paper. The so-called under-load effect much of the retardation disappears if a very low level minimum stress follows the overload, is also of importance for a prediction model to work properly under random load spectrum. The concept of Interactive Zone (IZ) fully considering reversed plasticity during unloading was discussed. This IZ concept can be combined with existing models to derive some improved models that can naturally take account of the under-load effect. Some simulations by IZ improved models for test under complex load sequences including multiple overloads and both over/under loads are compared with test results. It is seen that the improvement by IZ concept greatly enhanced the ability of existing models to accommodate complex load interaction effects.