• Journal of Microbiology and Biotechnology
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• v.21 no.12
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• pp.1250-1256
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• 2011

In this study, Bacillus licheniformis cells were immobilized by entrapment in calcium alginate beads and were used for production of alkaline protease by repeated batch process. In order to increase the stability of the beads, the immobilization procedure was optimized by statistical full factorial method, by which three factors including alginate type, calcium chloride concentration, and agitation speed were studied. Optimization of the enzyme production medium, by the Taguchi method, was also studied. The obtained results showed that optimization of the cell immobilization procedure and medium constituents significantly enhanced the production of alkaline protease. In comparison with the free-cell culture in pre-optimized medium, about 7.3-fold higher productivity was resulted after optimization of the overall procedure. Repeated batch mode of operation, using optimized conditions, resulted in continuous production of the alkaline protease for 13 batches in 19 days.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2007.05a
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• pp.235-240
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• 2007

A large power fuel cell generation system needs a parallel operation of de-de boost converter. Therefore, this paper proposed parallel operation algorithms of de-de boost converters for the large scale fuel cell generation system of 250[kW] and the operating principle along with the control method in detail. This paper uses a maximum current sharing method as a parallel operation method and also the phase shift full bridge de-de converter as a de-de boost converter. Simulation and experimental results on two prototype converter modules of 500W show that the parallel operation method can be applied to the 250[kW] power converter.

• Journal of the Optical Society of Korea
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• v.16 no.1
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• pp.42-46
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• 2012

We have developed a fluorescence optical detection system using a digital micromirror device (DMD) for monitoring 3D cell culture matrices in situ. Full 3D imaging with fast scanning speed was implemented by the combined action of a DMD and a motorized stage. Imaging results with fluorescent microbeads measure the minimum axial resolution of the system as $6.3{\mu}m$, while full 1-mm scanning through 3D alginate-based matrix was demonstrated. For cell imaging, improved images were obtained by removing background fluorescence although the scanning distance was reduced because of low intracellular fluorescence efficiency. The system is expected to be useful to study various dynamics and behaviors of 3-dimensionally cultured cells in microfluidic systems.

• Journal of the Optical Society of Korea
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• v.5 no.2
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• pp.39-42
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• 2001

A new method of switching mode in AFLC cell is proposed for faster switching time and higher contrast ratio. In this mode the ″dark″ state is obtained by applying negative full voltage while the ″bright″ state is achieved by applying positive full voltage to the cell. The switching time is reduced to 100 $mutextrm{s}$ for the cell whose switching time is 22 ms when operated in conventional mode. The contrast ratio is also improved vastly with this method. The possibility of achieving gray scale was shown in this mode of operation.

• Journal of the Korean Electrochemical Society
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• v.5 no.4
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• pp.173-177
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• 2002

Electrochemical characteristics of a graphite/lithium and a $LiCoO_2/lithium$ half cell and a $graphite/LiCoO_2$ full cell were analyzed using a GCSOC (gradual control test of the state of charge) technique. The IIE (initial intercalation coulombic efficiency), which represents lithium intercalation property of the electrode material, and the $lIC_s$ (initial irreversible capacity by the surface), which represents irreversible reaction between the electrode surface and the electrolyte were obtained from the GCSOC analysis. Linear-fittable capacity ranges of IIE of graphite and $LiCoO_2$ electrodes were 370 and 150 mAh/g, respectively, based on material weight. The value of lIE for graphite and $LiCoO_2$ electrodes were $93-94\%$ and $94-95\%$, respectively. The value of IICs for graphite and $LiCoO_2$ electrodes were 15-17 mAh/g and 0.3-1.7 mAh/g, respectively. The value of IIE for $graphite/LiCoO_2$ full cell, used GX25 and DJG311 as a graphite, was $89-90\%$ that lower than that for the half cells. Parameters of IIE and IICs can also be used to represent not only half cell but also full cell.

• Journal of computational fluids engineering
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• v.19 no.1
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• pp.47-56
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• 2014

Since a typical plate heat exchanger is made up of a huge number of unitary cells, it may be impossible to predict the aero-thermal performance of the full scale heat exchanger through three-dimensional numerical simulation due to the enormous amount of computing resources and time required. In the present study, a simple flow-network model using the friction factor correlation and a thermal-network model based on the effectiveness-number of transfer units (${\varepsilon}$-NTU) method has been developed. The complicated flow pattern inside the cross-corrugated heat exchanger has been modeled into flow and thermal networks. Using this model, the heat transfer between neighboring streams can be considered, and the pressure drop and the heat transfer rate of full-scale heat exchanger matrix are calculated. In the calculation, the aero-thermal performance of each unitary cell of the heat exchanger matrix was evaluated using correlations of the Fanning friction factor f and the Nusselt number Nu, which were calculated by unitary-cell CFD model.

• Journal of Power Electronics
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• v.16 no.3
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• pp.872-882
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• 2016

A novel pseudo-continuous conduction mode (PCCM) voltage-fed single-stage power factor correction (PFC) full-bridge battery charger is proposed in this paper. By connecting a freewheeling transistor in parallel with an input inductor, the PFC cell can operate in the PCCM with a constant duty ratio. Thus, the dc/dc stage can be designed using this constant duty ratio and the restriction on the duty ratio of the PFC cell is eliminated. As a result, the input current distortion is less and the dc bus voltage becomes controllable over the wide output power range of the battery charger. Moreover, the operation principle of the dc/dc stage is designed to be similar to that of a conventional phase-shifted full-bridge converter. Therefore, it is easy to implement. In this paper, the operation of the new converter is explained, and the design considerations of the controller and key parameters are presented. Simulation and experimental results obtained from a 1 kW prototype are given to confirm the operation of the proposed converter.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2005.11a
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• pp.371-376
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• 2005

In this paper, a new a new full-bridge soft-switching phase shift PWM DC-DC Converter has been proposed, which is suitable for fuel cell based power generation system. The proposed converter has outstanding advantage over the conventional DC-DC converter with respect to high efficiency, high power density, and hish component utilization. In special. the proposed converter has predominant high boosting output voltage and high efficiency characteristics under the inherently severs low output voltage of the fuel cell through the overall load conditions. Moreover, the developed converter has been experimentally tested with the help of a fuel cell simulator, and can generate the V-I characteristics of proton exchange membrane(PEM) fuel cell, so that the performance of the proposed converter could be effectively examined and the validity of the converter could be verified.

• Journal of Communications and Networks
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• v.11 no.1
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• pp.26-35
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• 2009

In this paper, we investigate how to do resource allocation to guarantee a minimum user data rate at low signaling overhead in multi-cell orthogonal frequency division multiple access (OFDMA) wireless systems. We devise dynamic resource allocation (DRA) algorithms that can minimize the QoS violation ratio (i.e., the ratio of the number of users who fail to get the requested data rate to the total number of users in the overall network). We assume an OFDMA system that allows dynamic control of frequency reuse factor (FRF) of each sub-carrier. The proposed DRA algorithms determine the FRFs of the sub-carriers and allocate them to the users adaptively based on inter-cell interference and load distribution. In order to reduce the signaling overhead, we adopt a hierarchical resource allocation architecture which divides the resource allocation decision into the inter-cell coordinator (ICC) and the base station (BS) levels. We limit the information available at the ICC only to the load of each cell, that is, the total number of sub-carriers required for supporting the data rate requirement of all the users. We then present the DRA with limited coordination (DRA-LC) algorithm where the ICC performs load-adaptive inter-cell resource allocation with the limited information while the BS performs intra-cell resource allocation with full information about its own cell. For performance comparison, we design a centralized algorithm called DRA with full coordination (DRA-FC). Simulation results reveal that the DRA-LC algorithm can perform close to the DRA-FC algorithm at very low signaling overhead. In addition, it turns out to improve the QoS performance of the cell-boundary users, and achieve a better fairness among neighboring cells under non-uniform load distribution.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.57 no.6
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• pp.982-984
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• 2008

This paper suggests anon-isolated high-gain boost converter using voltage-stacking cell. The voltage gain can be increased by adjusting number of voltage-stacking cells and transformer turns-ratio. Test results with 1kW prototype converter show that the voltage gain is three or four times higher than conventional boost converter at unity transformer turns-ratio and about 90% of efficiency is recorded under full load condition.