• Asian-Australasian Journal of Animal Sciences
• /
• 제20권3호
• /
• pp.351-358
• /
• 2007

Carbohydrate-protein interactions are known to be important in gamete interactions. Several evidence indicated that a fucose-containing sulfated polysaccharide fucoidan was potential inhibitor of fertilization in vitro and thus fucose seemed to be part of the recognition signal of gamete interaction in mammals. In recent investigation we found that ${\alpha}$-L-fucosidase activity was present in boar spermatozoa and it was related to sperm binding to and penetration into zona pellucida (ZP) in vitro. The objective of this study was to determine the effects of monosaccharide L-fucose and polysaccharide fucoidan on sperm ${\alpha}$-L-fucosidase activity and relation to sperm-oocyte interaction in pig. Results indicated that the activity of sperm ${\alpha}$-L-fucosidase was largely inhibited (62%) when sperm suspension was treated with monosaccharide L-fucose. It also significantly inhibited the number of sperm binding to ZP (32%) and penetration into zona-intact oocytes (72%), but did not inhibit penetration into zona-free oocytes when fertilization medium contained L-fucose. The chlorotetracycline (CTC) assessment showed that L-fucose did not affect induction of sperm capacitation and acrosome reaction. In contrast, the activity of sperm ${\alpha}$-L-fucosidase was not inhibited when sperm suspension was treated with polysaccharide fucoidan but sperm-ZP binding was greatly inhibited (85%) and completely blocked sperm penetration into zona-intact or zona-free oocytes. The CTC assessment showed that fucoidan increased the F pattern and decreased the AR pattern sperm. These results suggested that the different inhibitory mechanisms were present between monosaccharide L-fucose and polysaccharide fucoidan on sperm-oocyte interaction, the inhibition effect of ${\alpha}$-L-fucose on sperm binding and penetrating into ZP caused sperm ${\alpha}$-L-fucosidase inhibited by ${\alpha}$-L-fucose.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권17호
• /
• pp.7497-7500
• /
• 2015

Background: Tumor markers, designated as a broad group of substances produced by malignancies, could be in the form of biochemical substances, immunological substances, cell surface changes and genetic alterations. Cancer, a disorder of cellular behavior is characterized by alteration of serum glycoproteins. L-fucose, a hexose, which is the terminal sugar in most of the plasma glycoproteins, may be useful as a tumor marker for the detection, monitoring and prognostic assessment of malignancies. The aim of the study was to ascertain the role of serum fucose as a biomarker for early detection of oral cancer and to compare serum fucose levels in healthy controls, leukoplakia and oral cancer patients. Materials and Methods: The study included 60 (100.0%) subjects, who were grouped as 20 (33.3%) control subjects, 20 (33.3%) squamous cell carcinoma patients and 20 (33.3%) leukoplakia patients. Fucose estimation was done using UV-visible spectrophotometry based on the method as adopted by Winzler using cysteine reagent. The results were analyzed statistically using ANOVA with Bonferroni post hoc tests. Results: Results showed a high significance in serum fucose in oral squamous cell carcinoma (OSCC) and leukoplakia subjects compared to normal controls. There was a gradual increase in the values noted from control to leukoplakia and to squamous cell carcinoma. Conclusions: Estimation of serum fucose may be a reliable marker and can be used as an effective diagnostic biomarker in oral squamous cell carcinoma patients.

• 한국토양비료학회지
• /
• 제23권1호
• /
• pp.21-25
• /
• 1990

볏짚, 보리짚, 산야초(山野草) 그리고 활엽수(闊葉樹)와 침엽수(針葉樹)의 낙엽(落葉)을 채취(採取)하여 실험실(實驗室) 조건하(條件下)에서 90일간(日間) 부숙(腐熟)시키면서 경시적(經時的)으로 재료(材料)를 취(取)하여 산가수분해(酸加水分解)한 후(後) 단당류(單糖類)의 함량(含量)을 분석(分析)한 결과(結果)는 다음과 같다. 1. 부숙기간(腐熟其間)이 경과(經過)함에 따라 유기물중(有機物中) 단당류(單糖類)의 함량(含量)이 감소(減少)하였다. 중간단계(中間段階)(45일(日))에서는 일시적인 함량(含量)이 증가(增加)가 나타났다. 2. 보리짚이 볏짚보다 빠른 속도(速度) 부숙(腐熟)하였는데 활엽수낙엽(闊葉樹落葉)이 침엽수낙엽((針葉樹落葉)보다 빨리 부숙(腐熟)되었다. 3. 단당류(單糖類)의 함량(含量)은 산야초(山野草)나 산림낙엽(山林落葉)보다 볏짚 및 보리짚에 더 많았다. 4. 식물성(植物性) 단당류(單糖類)와 미생물성(微生物性) 단당류(單糖類)의 정량적(定量的) 구별(區別)은 불가능(不可能)하였다. 5. 단당류중(單糖類中) Glucose가 가장 많았으며 가장 적게 함유(含有)된 당(糖)은 Fucose이었다. Ribose는 검출되지 않았다. 6. 부숙기간(腐熟期間)이 경과(經過)함에 따라 Glactose의 상대적(相對的) 함량(含量)도 증가(增加)하였다. 7. 부숙기간(腐熟期間)이 증가(增加)함에 따라 Fucose의 상대적(相對的) 함량(含量)이 감소(減少)하였으며, Rhamnose도 이와같은 경향이었다. 다만, 볏짚의 경우 Rhamnose의 상대적(相對的) 함량(含量)은 오히려 증가(增加)하였다. 8. 부숙(腐熟) 90일경 각(各) 시료(試料)가 함유(含有)하고 있는 단당류(單糖類)의 양(量)은 다음과 같은 순서이었다. 볏짚 : Glucose > Xylose > Arabinose > Galactose > Rhamnose > Mannose > Fucose 보리짚 : Glucose > Xylose > Arabinose > Galactose> Mannose > Rhamnose > Fucose 산야초 : Glucose > Xylose > Galactose Arabinose > Rhamnose Mannose > Fucose 활엽수낙엽 : Glucose > Arabinose> Xylose Galactose > Mannose > Rhamnose = Fucose 침엽수낙엽 : Glucose > Xylose > Galactose > Arabinose Mannose > Rhamnose > Fucose.

• Journal of Microbiology and Biotechnology
• /
• 제28권7호
• /
• pp.1141-1146
• /
• 2018

2'-Fucosyllactose (2'-FL) is one of the most important human milk oligosaccharides and has several health benefits for infants. The levels of 2'-FL in breast milk or samples from other sources can be quantified by high-performance liquid chromatography. However, this method cannot be used for simultaneous detection of the target compound in numerous samples. Here, we developed a simple method for quantifying 2'-FL in a microplate format. The method involves two steps: (i) release of $\text\tiny{L}$-fucose from 2'-FL by ${\alpha}$-(1-2,3,4,6)-$\text\tiny{L}$-fucosidase and (ii) measurement of NADPH formed during the oxidation of $\text\tiny{L}$-fucose by $\text\tiny{L}$-fucose dehydrogenase. This method enables measurement of up to 5 g/l 2'-FL in 50 min using a 96-well microplate. The efficiency and simplicity of the proposed method make it suitable for the analyses of a large number of samples simultaneously.

• 한국수산과학회지
• /
• 제30권1호
• /
• pp.128-131
• /
• 1997

국내산 주요 갈조류인 미역, 다시마, 톳, 모자반 정제 fucoidan의 기본 결합 구조 및 황산기 결합 위치를 확인하기 위하여 정제 fucoidan및 이를 methanolysis하여 얻은 고분자 획분을 각각 methylation 및 FT-IR 측정을 하였다. 다시마, 톳, 모자반, 미역포자엽 정제 fucoidan의 주 결합은 $(1\rightarrow3)$ linked fucose였으며 미역포자엽에는 $(1\rightarrow3)$ linked galactose도 상당량 존재하였다. 황산기의 대부분은 fucose와 galactose의 C-4의 위치에 결합되어 있는 것으로 추정된다.

• 한국발생생물학회지:발생과생식
• /
• 제5권2호
• /
• pp.167-173
• /
• 2001

Ulex europaeus agglutinin I(UEA I), succinylated wheat germ agglutinin(sWGA), Griffonia simplicifolia lectin-I(GSL-I)을 이용하여 생쥐 부정소 조직내 당쇄의 분포를 조사하였다. 당쇄의 측쇄 말단의 $\alpha$-L-fucose 잔기에 특이적으로 결합하는 UEA I은 체부 및 미부 부정소를 제외한 두부 부정소 선단의 세정관 상피를 강하게 표지하였으나 관강은 중간 정도의 강도로 모두 표지되어 $\alpha$-L-fucose 잔기를 갖는 부정소 항원들은 부정소 선단부에서 주로 합성된 후 분비되는 것으로 추정된다. 이와 반대로 당쇄 말단의 $\alpha$-D-galactose 잔기에 특이적으로 결합하는 GSL-I은 두부 부정소를 제외한 체부와 미부 부정소 상피의 투명세포의 세포질과 섬모 및 기저세포를 표지하였다. 다당 사슬 및 복잡한 구조를 갖는 glycan의 당쇄의 N-acetyl-giucosamine 잔기에 특이적으로 결합하는sWCA는 부정소 부위별로 커다란 차이를 보이지 않았으나, 체부와 미부 부정소의 투명세포는 주세포에 비해 더 강하게 표지되어 sWGA 표지는 투명세포 기능분화의 표식자로 추측된다. UEA I 및 sWGA에 의한 관강의 표지 강도는 체부보다는 미부에서 약하게 관찰되어 미부 부정소 관강내에 $\alpha$-L-fucose 및 N-acetyl-glucosamine 잔기의 절단과 관련된 효소활성이 존재하는 것으로 추정된다. 요약하면 $\alpha$-L-fucose, $\alpha$-D-galactose, N-acetyl-glucosamine 잔기를 갖는 당쇄의 분포가 상피세포의 종류 및 부정소의 길이를 따라 차이를 보임을 확인하였다. 이는 정자의 성숙을 조절하는 부정소 각 절편 및 특정 절편 내 소관상피 세포의 기능적 분화를 대변하는 것으로 사료된다.

• 한국식품영양과학회지
• /
• 제26권1호
• /
• pp.72-80
• /
• 1997

식용 및 약용으로 이용한 역사가 오래인데도 불구하고 구성성분의 조성에 대하여 충분한 연구가 되어 있지 않은 해삼에서 당단백질과 황산콘드로이친을 추출정량하여 이들의 화학적 조성과 구조적 특성을 GC. MS및 IR로 검토하였다. 동결건조 해삼분말의 화학적인 조성은 sulfate esters함량이 0.90~l.21%, 다당류 함량이 23.08~26.97%였고 이를 구성하고 있는 구성단당류는 fucose의 함량이 30%이상을 차지하는 것을 비롯하여 glucose, N-acetylglucosamine, mannuronic acid, ribose의 5종이 거의 88%이상을 차지하고 있었고 그외 mannose, galactose, N-acetylgalactosaminne, D-glucuronic acid도 소량 검출되었다. 추출당단백질의 sulfate esters의 함량은 0.96~l.13%, 다당류의 함량은 58.7%~62.7%로 나타나고 주요 구성당은 fucose가 30%내외로 가장 많았으며 mannose, glucose와 ribose가 86%이상을 차지하며 galactose, N-acetylgalactosamine, D-glucuronic acid, N-acetylglucosamine도 소량 함유하고 있었다. 동결건조 해삼분말에서 추출한 황산콘드이친의 황산기의 함량은 3.52~3.72%이었다. 이들의 구성성분은 fucose, N-acetylgalactosamine 및 D-glucuronic acid 이며 IR spectrum에서 1240$cm^{-1}$ /의 S=0신축진동과 850$cm^{-1}$ /나 820$cm^{-1}$ /의 C-O-S 신축진동이 나타나 fucose의 $C_4$의 결합위치를 나타내고 있었다.

• Journal of Microbiology and Biotechnology
• /
• 제18권3호
• /
• pp.434-442
• /
• 2008

We observed that an inclusion body (IB) of recombinant ${\beta}$-galactosidase that was produced by the araBAD promoter system in Escherichia coli (E. coil) showed enzyme activity. In order to improve its activity, the lowering of the transcription rate of the ${\beta}$-galactosidase structural gene was attempted through competition between an inducer (L-arabinose) and an inducer analog (D-fucose). In the deep-well microtiter plate culture and lab-scale fermentor culture, it was demonstrated that the addition of D-fucose caused an improvement in specific ${\beta}$-galactosidase production, although ${\beta}$-galactosidase was produced as an IB. In particular, the addition of D-fucose after induction led to an increase in the specific activity of ${\beta}$-galactosidase IB. Finally, we confirmed that the addition of D-fucose after induction caused changes in the structure of ${\beta}$-galactosidase IB, with higher enzyme activity. Based on these results, we expect that an improved enzyme IB will be used as a biocatalyst of the enzyme bioprocess, because an enzyme IB can be purified easily and has physical durability.

• Journal of Microbiology
• /
• 제38권1호
• /
• pp.1-7
• /
• 2000

Eight strains of Aeromonas hydrophila isolated from diseased trout in Korea were characterized and compared with an American type strain by various methods including biochemical and physiological tests, PCR, randomly amplified polymorphic DNA (RAPD), plasmid profiling, and gel electrophoresis of total, membrane, and extracellular proteins. Virulence factors such as surface array proteins, cytotoxin, hemolysin, haemagglutinin, and protease were also investigated. The Korean strains showed heterogeneity in Iysine decarboxylase production, utilization of various carbon sources, and production of acetoin. Five strains had the same profiles of total and membrane proteins. Six strains haemagglutinated with trout red blood cells (RBCs) which was inhibited by fucose, galactose, and mannose, except for No. 1 where haemagglutination was inhibited by only galactose and mannose, but not by fucose. Four isolates haemagglutinated with human RBCs which was inhibited by fucose and mannose yet not by galactose. The type strain haemagglutinated only with trout RBCs which was inhibited by fucose, galactose, and mannose. Every isolate secreted protease, hemolysin, cytotoxin, and siderophore, but no enterotoxin. Results showed that the Korean isolates, except for No.7, had very different biochemical and molecular characteristics from those of the American type strain.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권3호
• /
• pp.1125-1131
• /
• 2014

Background: Schistosomiasis is a parasitic disease causing chronic ill health in humans with a serious consequences for socio-economic development in tropical and subtropical regions. There is also evidence linking Schistosoma mansoni to colonic carcinoma occurrence. The aim of this study was to evaluate some inflammatory and oxidative stress biomarkers, as well as L-fucose as linkers between intestinal schistosomiasis and colonic dysplasia development in mice. Materials and Methods: This study was conducted upon 80 mice that were divided the control group (10 non infected mice) and infected group which was subdivided into 7 sub-groups (10 mice each) according to the time of sacrifaction in the post infection (p.i.) period, 10 mice being sacrificed every two weeks from 6 weeks p.i. to 18 weeks p.i. Tumor necrosis factor alpha (TNF-${\alpha}$), inducible nitric oxide synthase (iNOS), and pentraxin 3 (PTX3) levels were estimated by immunoassay. The L-fucose level, and thioredoxin reductase (TrxR) and lactate dehydrogenase (LDH) activities were also evaluated in colonic tissue. Results: The current study revealed statistically significant elevation in the studied biochemical markers especially at 16 and 18 weeks p.i. The results were confirmed by histopathological examination that revealed atypical architectural and cytological changes in the form of epithelial surface serration and nuclear hyper-chromatizia at 14, 16 and 18 weeks p.i. Conclusions: inflammation, oxidative stress and L-fucose together may form an important link between Schistosomal mansoni infection and colonic dysplasia and they can be new tools for prediction of colonic dysplasia development in experimental schistosomiasis.