• Mycobiology
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• v.43 no.1
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• pp.24-30
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• 2015

The effects of monokaryotic strains on fruiting body formation of Lentinula edodes were examined through mating and cultivation of the mated dikaryotic mycelia in sawdust medium. To accomplish this, monokaryotic strains of L. edodes were isolated from basidiospores of the commercial dikaryotic strains, Chamaram (Cham) and Sanjo701 (SJ701). A total of 703 matings (538 self-matings and 165 outcrosses) were performed, which generated 133 self-mates and 84 outcross mates. The mating rate was 25% and 50% for self-mating and outcross, respectively. The bipolarity of the outcross indicated the multi-allelic nature of the mating type genes. The mating was only dependent on the A mating type locus, while the B locus showed no effect, implying that the B locus is multi-allelic. Next, 145 selected dikaryotic mates were cultivated in sawdust medium. The self-mated dikaryotic progenies showed 51.3% and 69.5% fruiting rates for Cham and SJ701, respectively, while the fruiting rate of the outcross mates was 63.2%. The dikaryotic mates generated by mating with one of the monokaryotic strains, including A20, B2, E1, and E3, showed good fruiting performance and tended to yield high fruiting body production, while many of the monokaryotic strains failed to form fruiting bodies. Overall, these findings suggest that certain monokaryotic strains have traits enabling better mating and fruiting.

• Journal of Ecology and Environment
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• v.41 no.9
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• pp.256-264
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• 2017

Background: The aim of this study was to clarify the relationship between the sexual reproduction and the resource allocation in a natural Polygonatum humile population grown in a temperate mixed forest gap. For this aim, the plant size, the node which flower was formed, the fruiting rate, and the dry weight of each organ were monitored from June 2014 to August 2015. Results: Firstly, in 3-13-leaf plants, plants with leaves ${\leq}8$ did not have flowers and in plants with over 9 leaves the flowering rate increased with the number of leaves. Among plants with the same number of leaves, the total leaf area and dry weight of flowering plants were larger than those of non-flowering plants. The minimum leaf area and dry weight of flowering plants were $100cm^2$ and 200 mg, respectively. Secondary, the flowers were formed at the 3rd~8th nodes, and the flowering rate was highest at the 5th node. Thirdly, cumulative values of leaf properties from the last leaf (the top leaf on a stem) to the same leaf rank were greater in a plant with a reproductive organ than in a plant without a reproductive organ. Fourthly, fruit set was 6.1% and faithful fruit was 2.6% of total flowers. Biomasses of new rhizomes produced per milligram dry weight of leaf were $0.397{\pm}190mg$ in plants that set fruit and $0.520{\pm}0.263mg$ in plants that did not, and the difference between the 2 plant groups was significant at the 0.1% level. Conclusions: P. humile showed that the 1st flower formed on the 3rd node from the shoot's base. And P. humile showed the minimum plant size needed in fruiting, and fruiting restricted the growth of new rhizomes. However, the fruiting rate was very low. Thus, it was thought that the low fruiting rate caused more energy to invest in the rhizomes, leading to a longer rhizome. A longer rhizome was thought to be more advantageous than a short one to avoid the shading.

• Journal of Korean Society of Forest Science
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• v.100 no.3
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• pp.432-440
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• 2011

The objective of this study was to investigate growth and fruiting characteristics (e.g., nut qualities) of chestnut (Castanea cerenata) after applying various fertilizer treatments at the cultivation site in Suncheon. Fertilizer treatments were designed as follow: liquid fertilizer, chemical fertilizer, organic fertilizer, and control. Both liquid and chemical fertilizer treatments provided the best growth in height and basal diameter. In addition, these two treatments were very effective for crown width of the trees between both east-west and north-south orientation. The liquid fertilizer treatment was effective on total length of the fruiting branch and length of the bearing to terminate part. Both liquid and chemical fertilizer treatments produced the longest length of basal to bearing part compared to the other two treatments. The liquid fertilizer treatment showed the most thickened basal diameter of the fruiting branch and the greatest diameter of above and below the bearing burr part. Elongation Index of the fruiting branch (EI) was the highest with liquid fertilizer treatment and the remaining four indices (Production Index of fruiting branch, PI; Ratio of Diameter between below and above bearing burr part, RD; Growth Index of fruiting branch diameter, GI; Thickness Index of fruiting branch or dormant branch, TI) were the highest with the chemical fertilizer treatment. Total number of produced branch per fruiting mother branch and number of small and weak branches per fruiting mother branch were highest on the control and liquid fertilizer treatment; however, all treatments produced similar numbers. The chemical and organic fertilizer treatments produced a high number of fruiting branches per fruiting mother branch, while organic and liquid fertilizer treatments produced a high number of burr per fruiting branch. The rate of commercializing on the basis of nut weight and quantity was higher on control (87.5%) than chemical fertilizer treatment (84.6%); however, the rate was even lower on liquid fertilizer treatment (84.3%) and organic fertilizer treatment (82.7%). The liquid fertilizer treatment showed the highest average of nut weight, while chemical fertilizer treatment showed the highest average number of fruiting burr. There was no significant difference in average number of normal nuts per burr among treatments. The yield per tree was high on chemical (8.2 kg) and liquid (8.0 kg) fertilizer treatments, but there was no significant difference among treatments. In the rate of nut grade on the basis of nut weight and quantity, the liquid fertilizer treatment, 43.5% and 34.3% more than large nut respectively, produced higher value chestnuts compared to other treatments.

• Mycobiology
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• v.49 no.2
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• pp.161-172
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• 2021

Phellinus strains were collected from different areas in Korea. Of them, the fast mycelial growing strains were artificially cultivated on the oak logs to produce fruiting body. The varieties, Phellinus linteus ASI26099 (Korea Sanghwang) and P. baumii PBJS (Jangsoo Sanghwang) were grown under the same conditions as controls. Their cultivating characteristics including mycelial colonization, pinhead formation, and fruiting body formation rate were investigated on the logs. Basidiocarps of Phellinus strains HN00K9, HN6036, and ASI26099 were concentrically zonate and shallowly sulcate, and dark chestnut showing typical characteristics of Tropicoporus linteus (synonyum: P. linteus, Inonotus linteus, polyporus linteus), which is distinguishably different to PBJS. HN00K9 showed the highest yield of fruiting body among the mushroom strains. The β-glucan content in fruiting bodies of HN00K9 was 20% higher than those of other strains. Bioactive effects of polysaccharide samples from fruiting bodies of Phellinus strains, HN00K9, HN6036, ASI26099, and PBJS were assessed on cell viability and cytokine (IL-6 and TNF-α) inhibition and finally on anticancer to different human cancer cells.

• Korean Journal of Agricultural Science
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• v.13 no.1
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• pp.44-51
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• 1986

A total number of 916 fruiting bodies of Ganoderma lucidum (Fr.) Karsten grown naturally in Korea were collected to investigate the morphological characters and some other useful characters related to the quality of the fungus and the results obtained are summarized as follows; The host tree showing the highest parasitic rate by the Genoderma lucidum(Fr.) Karsten was oak tree and the parasitic rate of the tree was 86.2%. The order of parasitic rate of the host trees by the fungus next to the oak was peach, chestnut, persimmon and acasia. The size, shape and color of fruiting bodies were varied according to the host trees. The average size of fruiting bodies collected was 40 to 100 mm by 30 to 80mm. The fruiting bodies with pileus size of 60 by 45 mm, pileus thickness of over 10mm, pileus minor axis/stipe length ratio of over 0.65 and pileus thickness/pileus minor axis ratio of over 0.22 were considered having higher commerical values. The external shape of the fruiting body was very important criterion to evaluate the quality of the Ganoderma lucidum (Fr.) Karsten. A great variation in characters such as pileus shape, color, marginal shape, zonation of pileus, pileus thickness, poroid layer of fruiting bodies and shape of stipe were observed from the fruiting bodies of G. lucidum collected in Korea. Further studies will be necessary for the genetic nature of these characters.

• Journal of Bio-Environment Control
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• v.15 no.4
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• pp.385-389
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• 2006

This study was carried out to determine the effect of raising seedling at Hallasan area of sub-alpine (altitude of 600m above sea level) to improve fruiting.ate of Squash (Cucurbita maxima) in retarding culture. 'Ebis' cultivar was seeded in plug tray of 32 cells and the seedlings were grown for 25 days. They were transplanted on August 26, 2004, following L-stem training method under rain-shielding condition. Seedling height, number of nodes and leaf area were higher in lowland than in sub-alpine area. T/R ratio of seedling in sub-alpine was much lower as compared with that in the lowland. The first fruiting was on the 19th node in sub-alpine area, and on the 26th node in the lowland area(control). The succeeding fruiting nodes were lower by 3 to 5 node than those of control. Fruiting rate of second flower was improved by 17.2% compared with the 1.4% in control. The marketable yield was increased by 27% by raising seedling in sub-alpine area (4,460 kg/10a). This also brought out 20% labour saving effect. The environmental condition for raising seedling in the sub-alpine area of Hallasan was effective for the improvement of Squash (Cucurbita maxima) fruiting rate compared with lowland area.

• The Korean Journal of Mycology
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• v.38 no.1
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• pp.83-84
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• 2010

We inoculated the spawn of Inonotus obliquus on living trees of Betula platyphylla var. japonica, and could get several fruiting bodies from the inoculations. The fruiting body showed almost the same feature of that collected from natural habitats in Korea, while the size was much smaller than that of natural one. The diameter of the fruiting body ranged from 1.1 cm to 4.8 cm, and the height 0.5 cm to 2.0 cm. We expect that a harvest is possible within 5 years, but need to check the growth rate of the fruiting body to decide suitable time for getting more economic value.

• The Korean Journal of Mycology
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• v.51 no.2
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• pp.69-80
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• 2023

The cultivation conditions of shiitake mushrooms (Lentinula edodes) influence the production and quality of fruiting bodies. We conducted this study to improve the productivity and quality of shiitake mushrooms by modifying the cultivation conditions. Two types of spawns (sawdust and liquid spawn) were used, and corn flour was used as a nutritional source for the sawdust medium. A blue light-emitting diode (LED; 300 lux) was also used instead of a white LED during the incubation period. Sanbaekhyang was used as the experimental variety. When using corn flour, the mycelial growth rate increased 1.1 to 2.7 times the growth rate of the control up to 21 days of incubation, and the weight loss rate of the media was also higher. Mushroom productivity increased 1.2 times when the liquid spawn was used compared to when the sawdust spawn was used, and the blue LED also increased fruiting body production by 1.1 times compared to the white LED. Mushroom productivity increased when the liquid spawn was used, and the blue LED also increased fruiting body production. Fruiting body weight and the size of the cap were greater when sawdust spawn was used. The fruiting body weight and the stipe diameter were greater when the blue LED was used. Taste analysis showed that the saltiness increased when corn flour was used, and the sourness increased when the blue LED was used.

• The Korean Journal of Mycology
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• v.47 no.2
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• pp.143-152
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• 2019

A new cultivar 'Sanjanghyang' was bred from monokaryotic strains of 'Sanbaekhyang' and 'Jangan 1ho'. Pileus was flat, round, and reddish brown. The diameters of the pilei and stipe length of the fruiting bodies were 67.1 mm and 16.9 mm, respectively. The scales were white or slightly brown and distributed evenly. The gill density was sparse and showed a rippled texture. The stipe was cream in color and the fluff was medium. 'Sanjanghyang' had a short cultivation period and fruiting bodies occurred sporadically. Temperature for fruiting body formation was a medium, between 15 to $19^{\circ}C$. 'Sanjanghyang' was different from 'Sanbaekhyang' with regard to its pileus diameter (67.1 mm) and autumn and spring fruiting body production period. 'Sanbaekhyang' had pileus diameter of 74.7 mm, and fruiting body formation occurred in spring and autumn. The rate of fruiting body formation was 89% (first flush), 4% (second flush), and 7% (third flush).

• Mycobiology
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• v.39 no.4
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• pp.272-277
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• 2011

Chemical mutagenesis of basidiospores of Hypsizygus marmoreus generated new mushroom strains. The basidospores were treated with methanesulfonate methylester, an alkylating agent, to yield 400 mutant monokaryotic mycelia. Twenty fast-growing mycelia were selected and mated each other by hyphal fusion. Fifty out of the 190 matings were successful (mating rate of 26.3%), judged by the formation of clamp connections. The mutant dikaryons were cultivated to investigate their morphological and cultivation characteristics. Mutant strains No. 3 and No. 5 showed 10% and 6% increase in fruiting body production, respectively. Eight mutant strains showed delayed and reduced primordia formation, resulting in the reduced production yield with prolonged cultivation period. The number of the fruiting bodies of mutant No. 31, which displayed reduced primordial formation, was only 15, compared to the parental number of 65. Another interesting phenotype was a fruiting body with a flattened stipe and pileus. Dikaryons generated by mating with the mutant spore No. 14 produced flat fruiting bodies. Further molecular biological studies will provide details of the mechanism. This work shows that the chemical mutagenesis approach is highly utilizable in the development of mushroom strains as well as in the generation of resources for molecular genetic studies.