• Preventive Nutrition and Food Science
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• 제1권2호
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• pp.252-255
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• 1996

As an index of freeze-injury of yeast, the leakage of intracellular substances from yeast cells after freeze-thawing was investigated. It was found that much more ultraviolet-absorbing substances leaked out from non-freeze tolerant yeast (NETY) than from freeze-tolerant yeast. Furthermore, the rate of leakage of cellular substances form NFTY during incubation exceeded that of FTY, indicating that NFTY is more susceptible to freeze-injury than FTY during frozen-storage. An apparent degradation of phospholipid was observed during incubation of perfermented frozen-cells of NFTY, while little change of phospholipid occurred in FTY, These results suggested that the difference in the sensitivity of yeast might be due to the strength of cell membrane in terms of the degradation of phospholipid by enzymes, phospholipases, attached to cell membranes.

• 동아시아식생활학회지
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• 제25권5호
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• pp.893-901
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• 2015

This study investigated the effects of thawing conditions on physiological activities and quality of peeled garlic. Peeled frozen garlic was analyzed after thawing at low temperature ($4^{\circ}C$), room temperature ($20^{\circ}C$), tap water ($20^{\circ}C$), radio frequency of 27.12 MHz, and 2.45 GHz in a microwave. As a result, the time required to thaw garlic to $0^{\circ}C$ by various thawing methods was shortest at2.45 GHz in a microwave, followed by $20^{\circ}C$ tap water, radio frequency of 27.12 MHz, $20^{\circ}C$, and $4^{\circ}C$. Microwave thawing was faster than other methods, but it resulted in significant non-uniformity of heating. The hardness of peeled garlic significantly decreased upon freeze-thawing, whereas it showed improved hardness upon radio-frequency thawing. Total color difference in garlic increased upon freeze-thawing, and it was not improved by various thawing methods. Antioxidant activities were determined for DPPH radical scavenging ability, SOD-like activity, and reducing power. Total phenolic compounds and flavonoids in garlic extract were measured as $3.222{\pm}0.214{\mu}g$ GAE/g and $0.149{\pm}0.03{\mu}g$ QE/g, respectively. The content of total phenolic compounds was significantly reduced by 2.45 GHz microwave thawing ($1.90{\pm}0.02{\mu}g$ GAE/g); however, flavonoid contents were slightly reduced under freezing and thawing conditions. The DPPH radical scavenging ability of garlic extracts was not affected by thawing methods; however, SOD-like activity and reducing power were slightly reduced by freeze-thawing. These results indicate that physiological activities were not improved by radio-frequency thawing; however, thawing time and maintain hardness were reduced compared with conventional thawing methods.

• 한국식생활문화학회지
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• 제21권4호
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• pp.426-432
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• 2006

This study was conducted to investigate the changes in quality characteristics of frozen maesil according to thawing methods. The quality of maesil thawed in microwave oven was superior to those thaw in refrigerating temperature(5 $^{\circ}$) and in room temperature(25 $^{\circ}$). Drip loss of maesil thawed in microwave oven was 3.2${\pm}$0.2%. The total content of free sugars of maesil was 426.6 mg%, and 3% of them was decreased during thawing in microwave oven. The total content of organic acids was 5,297.2 mg%, and 2.5% of them was decreased during thawing in microwave oven. The total content of free amino acids was 281.4 mg%, and 2.1% of them was decreased during thawing in microwave oven. The principle ingredients of frozen maesil was stand for the lost contents of free sugar and a content loss of free organic acid and free amino acid were the fewest by thawing. Antioxidant effect for soybean oil and linoleic acid of maesil extract were expressd POV and TBA values. Antioxidative activity of fresh maesil extract was highest followed by maesil thawed in microwave oven, thawed in refreezing temperature (5$^{\circ}$)and room temperature (25$^{\circ}$)

• Geomechanics and Engineering
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• 제18권2호
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• pp.189-203
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• 2019

Brine leakage is a common phenomenon during construction facilitated by artificial freezing technique, threatening the stability of frozen wall due to the continual thawing of already frozen domain. This paper takes the frequently encountered soft clay in Wujiang District as the study object, and remolded specimens were prepared by mixing calcium chloride solutions at five levels of concentration. Both the deformation and pore water pressure of frozen specimens during thawing were investigated by two-stage loading tests. Three sections were noted from the changes in the strain rate of specimens during thawing at the first-stage load, i.e., instantaneous, attenuated, and quasi-stable sections. During the second-stage loading, the deformation of post-thawed soils is closely correlated with the dissipation of pore water pressure. Two characteristic indexes were obtained including thaw-settlement coefficient and critical water content. The critical water content increases positively with salt content. The higher water content of soil leads to a larger thaw-settlement coefficient, especially at higher salt contents, based on which an empirical equation was proposed and verified. The normalized pore water pressure during thawing was found to dissipate slower at higher salt contents, with a longer duration to stabilize. Three physical indexes were experimentally determined such as freezing point, heat conductivity and water permeability. The freezing point decreases at higher salt contents, especially as more water is involved, like the changes in heat conductivity. The water permeability maintains within the same order at the considered range of salt contents, like the development of the coefficient of consolidation. The variation of the pore volume distribution also accounts for this.

• 한국식품과학회지
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• 제31권1호
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• pp.54-61
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• 1999

본 연구는 해동 중 발생하는 drip 손실, 미생물의 오염, 표면의 과열 등을 방지하면서 급속 해동 방법을 찾기 위해 동결 돈육에 여러 가지 해동 방법을 적용하였다. 4, 28, $50^{\circ}C$에서의 해동과 2,450, 915 MHz의 microwave를 이용하여 해동을 실시하였고, 915 MHz microwave 해동은 출력을 5, 10, 15 kW로 변화시키면서 단일 조사와 $120^{\circ}C$의 열풍을 함께 조사한 경우를 해동시간, 온도분포, drip 손실, 보수력, 표면색도, 총세균수를 측정하였다. 915 MHz microwave를 이용한 해동은 열전도에 의한 해동보다 2백배 이상 시간이 단축되었고, 2,450 MHz microwave를 이용한 해동 방법의 침투깊이의 제한에서 발생되는 문제를 개선시킬 수 있었으며 drip 손실, 보수력, 총세균수에 있어서 효과가 컸다. 저주파수(915 MHz), 저출력(5 kW)의 microwave와 대류 가열을 병용하여 품질 저하를 최소한으로 방지하면서 급속해동을 위한 산업적 이용이 가능한 것으로 기대할 수 있었다.

• 한국가축번식학회지
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• 제26권2호
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• pp.119-124
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• 2002

본 연구는 돼지에서 동결정액을 이용한 번식능력을 개선하기 위한 동결정액 포장재료의 효과를 구명하기 위하여 실시하였다. 본 시험에는 축산기술연구소 종축개량부 (충남, 성환)의 인공수정센터에서 사육중인 종모돈이 사용되었다. 기존의 돼지 동결정액 포장방법인 maxi-straw 동결정액 포장방법과 5$m\ell$빈 cryogenic-vial 및 aluminum-pack 포장방법을 비교한 결과 cryogenic-vial로 포장하여 액체질소 상단 15cm에서 동결한 후 52$^{\circ}C$ water bath에서 190초 융해한 방법이 기존의 maxi-straw 방법과 비슷한 결과를 나타내었다. Cyogenic-vial 포장방법의 동결-응해 방법을 설정하기 위하여 융해시간을 달리하여 시험한 결과 액체질소 상단 15cm에서 동결하고 52$^{\circ}C$에서 190초 간 융해하였을 때 정자운동성이 120초 및 150초 응해시 보다 우수하였다 (P<0.05). 그러나 정상첨체비율은 응해시간 간에 차이가 없었다. 52$^{\circ}C$에서 45초간 응해 한 maxi-straw 포장방법과 52$^{\circ}C$에서 190초 융해한 cryogenic-vial 포장방법간에 정액성상을 비교한 결과 총정자운동성과 정자의 빠르기는 maxi-straw가 우수하였다 (P<0.05). 그러나 직진성과 정상첨체비율은 두 포장방법간에 차이가 없었다. 동결정액 포장방법별 인공수정시 번식성적은 maxi-straw 동결정액이 cryogenic-vial 동결정 액보다 수태율, 분만율, 그리고 산자수가 높았으나 통계적 유의차는 인정되지 않았다. 이상의 결과를 종합해 보면 cryogenic-vial 포장 방법의 동결 및 음해방법을 좀 더 연구개발하면 기존의 maxi-straw포장방법을 대체하여 실용화 할 수 있을 것으로 사료된다.

• 한국가축번식학회지
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• 제10권2호
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• pp.175-181
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• 1986

These experiments were carried out to clarify the effects various kinds of cryoprotectants which were frequently used in freezing embryos of domestic animals on the survival of frozen-thawed mouse embryos. As cryoprotectant, glycerol, DMSO and methanol were used and the procedures of adding them in medium were practiced by one-step or six-step adding method. Morphologically normal mouse embryos developed to blastocyst by in vitro culture after freezing and thawing were transferred to pseudopregnant recipients by surgical procedures. The results obtained in these experiments were summarized as follows: 1. The survival rates of the frozen-thawed 8-cell embryos, morulas and blastocysts following one-step addition of glycerol were 83.6, 80.3 adn 70.3%, respectively, while following six-step addition of glycerol, 69.2, 56.3 and 66.7% respectively. 2. When glycerol, DMSO and methanol were used as cryoprotectant under the same condition of freezing and thawing, the survival rates of frozen-thawed embryos were 74.0, 76.1 and 37.6%, respectively. 3. The implantation rate of embryos transferred to pseudopregnant recipients after freezing and thawing was 49.2%.

• 한국식품영양학회지
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• 제25권3호
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• pp.691-696
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• 2012

본 연구에서는 냉동 온도(-20, -30, -40, -50, -60 및 -70)와 해동 온도(4, 10, 20, 30, 40 및 $50^{\circ}C$)에 따른 마쇄 홍고추의 ascorbic acid, capsaicinoids, 유리당 함량과 ASTA 값의 품질 변화를 분석하였다. 냉동 온도에 따른 ascorbic acid 량은 67.08~80.35 mg/100 g 범위로 원료 값에 비해 57.00~64.10% 감소하였으며, 냉동 온도가 낮을수록 감소량은 적었다. 냉동 온도에 따른 capsaicinoids 함량, 유리당 함량 및 STA 값은 냉동 온도에 따른 차이를 보이지 않았고, 원료 값과도 유의적인 차이를 나타내지 않았다. 해동 온도에 따른 scorbic acid 함량은 70.34~75.90 mg/100 g 범위로 원료 값에 비해 45.12~52.69% 감소하였으며, 해동 온도가 높을수록 감소폭은 큰 경향을 보였다. 해동 온도에 따른 capsaicinoids 함량 및 유리당 함량은 원료 값과 유의적인 차이를 보이지 않았고, ASTA 값은 행동 온도가 높을수록 감소하는 경향을 보였다. 해동 온도에 따른 마쇄 홍고추의 전자코 분석 결과, 향기패턴 변화는 $20^{\circ}C$ 이상의 해동 온도에서부터 원료의 향과는 명확히 구분되는 것으로 나타났다. 이상의 결과로부터 마쇄 홍고추 냉동시 냉동 온도는 $-20^{\circ}C$가 적합한 것으로 나타났으며, 해동 온도는 $10^{\circ}C$ 이하에서 실시하는 것이 품질특성 변화를 최소할 수 있을 것으로 생각된다.

• 동아시아식생활학회지
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• 제20권4호
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• pp.560-566
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• 2010

개량흑돼지(105~110 kg)를 도축한 후 약 18시간 냉장하고 등심육을 분할하여 진공 포장하고 $-20^{\circ}C$에서 1개월 및12개월 동결한 후 각각 $4^{\circ}C$에서 20시간 해동하였다. 해동 직후의 것(0-TP)과 해동 후 $2^{\circ}C$에서 2일간 냉장한 것(2-TP)의 물리화학적 및 기호적 특성을 비교하여 해동 후 냉장이 품질에 미치는 영향을 검토하였다. 개량흑돼지 등심육을 1개월간 동결하여 해동한 경우, 해동 직후의 0-TP보다 해동 후 냉장한 2-TP가 보수력 및 근원섬유 소편화 정도가 높아지고, 경도 및 씹힘성이 낮아졌으며, 가열육의 다즙성 및 종합적 기호도가 향상되었으나(p<0.05) 12개월 동결한 등심육은 해동 후 냉장에 의한 효과가 유의적으로 크지 않았다.

• 한국지반신소재학회논문집
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• 제14권3호
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• pp.43-51
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• 2015

우리나라와 같이 4계절이 뚜렷한 지역은 동결 융해반복에 따른 강성도의 감소가 민간 시설물 인접 사면이나 산비탈면에 발생하였을 경우 안전율이 설계기준에 미흡하게 되는 현상이 발생되어 강우나 강설에 의한 산사태가 발생하여 큰 인명 및 재산피해로 이어질 우려가 있다. 이 연구에서는 동결 융해 반복에 따른 산지 사면의 안정성 변화를 알아보기 위하여 산지 사면의 표토에 분포하는 통일 분류법상의 SP와 SM계열의 흙시료를 채취하여 공시체를 제작하고 직접전단실험을 통하여 동토전단강도에 대한 특성을 분석하고, 수치해석적 방법을 활용하여 동결융해의 반복작용에 따른 화강풍화토의 강도감소특성과 흙시료의 동결 융해 적용시의 사면의 안정성의 변화를 검토하였다. 그결과 최대 전단응력은 약 10%정도의 감소율을 보였고, 사면 안정성해석결과 비동결시료에 비하여 요구안전율 이하의 경우가 있음을 확인하였다.