• Title/Summary/Keyword: Fresh and dry weight

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Studies on tissue culture of medicinal plants (II) -Tissue cultures of Glycyrrhiza glabra L. var. glandulifera $R_{EG.}$ et $H_{ERDER}$- (약용식물(藥用植物)의 조직배양(組織培養)에 관(關)한 연구(硏究)(II) -이태리감초(甘草)의 조직배양(組織培養)-)

  • Yoo, Sung-Cho;Kim, Sung-Soon
    • Korean Journal of Pharmacognosy
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    • v.7 no.1
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    • pp.55-57
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    • 1976
  • The callus formation of Glycyrrhiza glabra L. var. $glandulifera\;R_{EG}.\;et\;H_{ERD}$. in tissue culture was promoted on Murashige and Skoog's basal solution supplemented with 40g/l of sucrose, 1mg/l of kinetin and 5mg/l of 2, 4-D. The fresh and dry weights of callus and glycyrrhizin contents in callus of the Glycyrrhiza glabra L. var. $glandulifera\;R_{EG}.\;et\;H_{ERD}$. were determined monthly up to 12 months and obtained the results as follows: 1.The fresh weight of formed callus was increased rapidly from 2 to 4 months but growing rate of callus was slow from 4 to 6 months. This indicates that the cell division of callus was most active during the first $2{\sim}3$ months. 2. Glycyrrhizin contents in callus were also increased but the contents were not related to the increased weight of callus.

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Performance evaluation of a subsurface drainage culvert system in converted paddy fields

  • Do, Jong Won;Park, Jongseok;Kim, Hyuntai;Lee, Kwangya;Shin, Hyungjin
    • Korean Journal of Agricultural Science
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    • v.47 no.2
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    • pp.263-273
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    • 2020
  • With the change of the agricultural environment (increased rice production, decreased rice consumption, and rice production policies), converting paddy fields into upland fields is an increasing trend. In terms of conversion into upland fields, subsurface drainage is one of the most important factors for good field crop growth. This study evaluates the performance of a subsurface drainage culvert system in paddy fields and reclaimed lands. The obtained results are briefly summarized as follows: 1) After a comparative evaluation of several subsurface drainage culvert systems, including excavated subsurface drainage and non-excavated subsurface drainage types, type 3 (non-excavated, perforated drain pipe 50 mm, filter mat B50 cm, subsoiling 70 cm and culvert spacing 5 m) shows relatively high values among four types in terms of effectiveness (subsurface discharge capability) and economic efficiency (construction cost). 2) Type 3 has proven that it is suitable for design standards of discharge capacity through field tests performed in paddy fields (three sites: Gong-geom, Gae-san, Juk-san) and reclaimed lands (two sites: Gum-ho, Mi-am). 3) In the experiment of Sesamum indicum growth according to the existence of a drainage system, Sesamum indicum growth with a subsurface drainage culvert system had good value in terms of plant shoot and root length, shoot fresh and dry weight, and root fresh and dry weight).

Influence of Physico.Chemical Properties of Root Substrates on the Growth of 'Maehyang' Strawberry Daughter Plants Produced by Bag Culture of Stock Plants (포트 충전용 상토의 물리.화학성이 플라스틱 백 재배를 통해 발생한 '매향' 딸기의 자묘 생육에 미치는 영향)

  • Choi, Jong-Myung;Park, Ji-Young;Ko, Kwan-Dal;Lee, Chi-Won W.
    • Korean Journal of Agricultural Science
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    • v.37 no.2
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    • pp.199-207
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    • 2010
  • This research was conducted to determine the influence of physiological and chemical properties of root substrates on the growth of the daughter plants of 'Maehyang' strawberry produced by bag culture. The daughter plants produced by stock plants during bag culture were individually separated and grown in 10-cm diameter plastic pots containing six different formulations of root substrates: a) 50% peatmoss plus 50% vermiculite (5:5 by volume, A), b) 70% peatmoss plus 30% perlite (7:3, B), c) 70% coir dust plus 30% perlite (7:3, C), d) mixture of 35% coir dust, 35% peatmoss, and 30% perlite (3.5:3.5:3.0, D), e) mixture of 20% rice hull, 70% coir dust, and 10% perlite (2:7:1, E), and f) 30% rice hull plus 70% coir dust (30:70, F). The container capacity and air filled porosity of the growing medium varied greatly among the six substrate formulations evaluated. The substrates E and F had less container capacity and higher air-holding spaces than the rest of the formulations. Therefore, these two formulations (E and F) may cause a problem in water management during the production of healthy daughter plants. The substrate formulations A, B, and D retained higher nitrogen (N) concentrations than other formulations containing coir dust or rice hull. The substrate formulations E and F which contained rice hull had lower N, phosphorus (P), and potassium(K) concentrations than other substrate formulations containing coir. The quality of the daughter plants grown in all six different substrate formulations was good with the crown diameters at around 10 mm. Fresh weights of the daughter plants grown in substrate formulations A, C, and D were higher than those obtained from B, E, and F. Dry weights of the daughter plants showed a similar trend. The daughter plants having high fresh and dry weights and increased crown diameter are in demand by the industry. For this reason, the substrate formulations A, C and D can readily be used as potting mixes during the production of 'Maehyang' strawberry transplants utilizing the bag culture system.

Effects of Nutrient Solution Composition and Cutting Size on Growth of Virus-free Sweet Potato Plant in Nutrient Film Technique (NFT 수경재배에서 양액 종류 및 삽수 크기가 고구마 바이러스 무병주 생육에 미치는 영향)

  • Yoo, Kyoung-Ran;Lee, Seung-Yeob;Bae, Jong-Hyang
    • Horticultural Science & Technology
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    • v.30 no.6
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    • pp.686-693
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    • 2012
  • To develop a technique for mass-propagation of virus-free sweet potato [Ipomoea batatas (L.) Lam.] plant using nutrient film technique (NFT), the growth characteristics of 4 cultivars as affected by nutrient solution composition and cutting size were investigated. 72 cells (35 mL/cell) plug trays filled with vermiculite and perlite (1:1, v/v) were used. Vine length, fresh and dry weights of virus-free plants were the greatest in the nutrient solution recommended by National Horticultural Research Station in Japan, followed by that recommended by National Institute of Horticultural & Herbal Science in Korea, and Yamazaki's nutrient solution for lettuce. The growth of uppershoot cuttings was the best among 4 subsections of cutting. Vine length, and fresh and dry weights increased in the longer cutting treatments, and were better in 'Shinzami' and 'Yeonhwangmi' than those in 'Mannami' and 'Shincheonmi'. Vine diameter and length of the longest root were not significantly affected by the cutting size and cutting source. The growth characteristics of the single node cutting were not significantly different from those in 2-node cutting. The efficiency of rapid mass-propagation could be promoted with single node cuttings and uppershoot cuttings grown in NFT system.

A Comparison between Diploid and Tetraploid Cultivars of Lolium multiflorum Lam, italicum (이탈리안 라이그라스의 2배체와 4배체 품종간 비교)

  • 박병훈;박병식;강정훈
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.7 no.3
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    • pp.135-139
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    • 1987
  • The influence of ploidy level on agronomic characteristics in italian ryegrass, Lolium multiflorum Lam, italicum, was studied using diploids (2x) and tetraploids (4x) on the upland of Livestock Experiment Station, Suweon. The results are summarized as follow: 1. Tetraploid cultivars are in general dark green in leaf color and strong glittered. 2. Diploid cultivars are more resistant to cold than tetraploids but degree of resistance to cold depends on cultivars. The di-and tetraploids did not differ in summer survival and coldand heat resistance of di-Itetraploid cultivars were not related. 3. Tetraploid cultivars have larger leaf-blades, more rapid growth and higher leaf ratio to stem by heading than diploids. 4. Tetraploids possess significantly lower dry matter content than diploids. 5 . Fresh- and dry weight of tetraploids are similar with that of diploids but tetraploids are more leafy than diploids. 6. Seasonal yield depends more on the type of the cultivar than on its tetraploid nature.

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Effects of Light-emitting Diodes on In Vitro Growth of Virus-free Sweet Potato Plantlets (LED가 고구마 바이러스 무병묘의 기내 생장에 미치는 영향)

  • Yoo, Kyoung-Ran;Lee, Seung-Yeob
    • Horticultural Science & Technology
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    • v.35 no.4
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    • pp.490-498
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    • 2017
  • The in vitro growth of virus-free sweet potato [Ipomoea batatas (L.) Lam.] plantlets was investigated under different light sources: fluorescent lamp (control); red (660 nm), blue (460 nm), white light-emitting diodes (LED), and two mixtures of blue and red LED (R:B = 8:2, and 7:3). Single node explants (10 mm) of three cultivars ('Matnami', 'Shincheonmi', and 'Yeonhwangmi') were cultured on Murashige and Skoog medium supplemented with $0.2mg{\cdot}L^{-1}$ 6-benzyladenine for 4 weeks. Explants were exposed to $150{\pm}5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ photosynthetic photon flux at a distance of 20 cm, constant temperature of $25^{\circ}C$, and under 16/8-h (day/night) photoperiod. Using the same method, the in vitro growth of 10 cultivars under red LED was also compared. After 3 weeks, vine length was highest in plantlets cultured under red LED, and lowest in plantlets cultured under blue LED. Fresh and dry weights were also greatest in plantlets cultured under red LED. Compared to the control, vine thickness was significantly higher in plantlets grown under white LED and the 7:3 R:B LED mixture. Significant differences were observed among the 10 cultivars grown under red LED. 'Matnami', 'Shincheonmi', and 'Shinhwangmi' all had excellent vine lengths, and fresh and dry weights. Compared to the control, vine elongation of sweet potato plantlets was most effective under red LED, and culture duration was about 1 week shorter.

Incorporation Effect of Green Manure Crops on Improvement of Soil Environment on Saemangeum Reclaimed Land during Silage Corn Cultivation

  • Yang, Chang-Hyu;Lee, Jang-Hee;Baek, Nan-Hyun;Shin, Pyeong;Cho, Kwang-Min;Lee, Sang-Bok;Lee, Gyeong-Bo
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.3
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    • pp.187-192
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    • 2013
  • This study was carried out to investigate the incorporation effect of green manure crops (GMC) such as the hairy vetch on improvement of soil environment in reclaimed land during silage corn cultivation over the past two years. Plots consisted of conventional fertilization (CF) and incorporation of GMC were divided by addition rate of nitrogen fertilizer (100 kg $ha^{-1}$) with 30 - 100% of non nitrogen fertilization (NNF). Soil physico-chemical properties and growth and yield potential of silage corn were examined. The tested soils showed strong alkali and saline properties with low contents of organic matter and available phosphate while contents of exchangeable sodium and magnesium were high. Soil salinity increased during cultivation of summer crop. However, corn was not affected by salt content. The fresh weight of GMC at incorporation time was 18,345 kg $ha^{-1}$. Content of total nitrogen was 3.09% and the C/N ratio was 12.8 at incorporation time. Fresh and dry matter yield of silage corn were higher in the order of N30% reduction, CF, N50% reduction, N70% reduction, N100% reduction and NNF. Fresh and dry matter yield potential of silage corn for N30% reduction were comparable to those of CF. Bulk density of the soil decreased with incorporation of GMC, while porosity was increased. The soil pH decreased while content of exchangeable calcium, available phosphate, and organic matter increased. Also contents of exchangeable sodium and potassium decreased with incorporation of GMC. The data indicate that incorporation of hairy vetch can improve soil physical and chemical properties and reduce nitrogen fertilizer application especially for alkali saline reclaimed soil such as Saemangeum reclaimed land.

Tuber Yield and Characteristics of Chinese Yam as Affected by Harvesting Date and Storage Condition (수확시기와 저장방법에 따른 마의 塊根重과 품질변화)

  • 김영광
    • Korean Journal of Plant Resources
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    • v.11 no.2
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    • pp.136-141
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    • 1998
  • To give some information on the harvest and storage of Chinese yam (Dioscorea opposita), the experiment was done to examine the effect of its harvesting date and storage method on tuber weight, tuberrelated characters during the storageor at the end of storage. The yam tubers harvested 4 times at 20 days interval from Oct. 15 were stored with different storage methods(pit, room and cold temperature) and on Mar.15. all the characters were measured. Fresh weight of the tuber harvestedafter Nov. 5 and stored were greater than that done on Oct. 15 while dry tuber weight did not have any difference between the harvesting dates. The fresh weigth done directly from the field on Mar. 15 was higher compared to the other harvesting dates owing to its higher moisture content. The rate of sound tuber stored at room or cold temperature condition was higher than that at the field at which the rate was greater at the harvest after late nov. compared to the earlier harvests. Although the two storages were similar at sound tuber rates and marketable yield, the tuber weight was more reduced when stored at room temperature than when done at cold temperature . Brightness and value 'b' of chromaticaity realted to the tuber coloring and sugar content were higher but moisture content was lowerwhen harvested on Oct. 15 than the other havesting dates. All the characteristics realted to the marketability after storage were nearly same when stored at room and cold temperature. Value 'a' of chromaticity and sugar content were higher when harvest on Mar. 15 than when harvested before Dec. 15 but the 'b' value and mositure content were reverse results.

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Effect of Seeding Date on Forage Yield and Chemical Composition of Echinochloa crusgalli var. Frumentacea(Roxb) Wight in Jeju Region (제주지역에서 파종기에 따른 청예피의 사료수량 및 조성분 변화)

  • 조남기;강영길;송창길;고영순;조영일
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.21 no.4
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    • pp.217-224
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    • 2001
  • In order to identify the growth characterization, the yield and chemical composition of Jeju barnyard grass (Echinochloa crusgalli var. fiurnentacea(Roxb) Wight) based on seeding date in Jeju region, seeding carried out the 10-day intervals from March 27 to September 30 in 2000, respectively. Plant height was 143.2 cm, showing the highest on seeding date, April 6 among that of any other seeding date. On the other hand, those of early and late seeding gradually decrease. Plant height was 119.2 an in May 16 seeding. The results of stem diameter, number of withering leaves, number of leaves and fresh weight per plant were similar to those of the plant heights. The yield of fresh, dry matter forage, crude protein and TDN found the highest on April 6 seeding, 63.5 MT/ha, 13.9 MTha, 1.1 MT/ha, and 7.6 MT/ha, respectively. In early and late seeding, the yield was gradually decreased. In seeding May 16, the yield found .38.2 MTIha in fresh forage, 6.2 MTha in dry matter forage, 0.7 MT/ha in crude protein and 3.7 MTha in TDN, respectively. According to delaying the seeding date, March 27 to May 16, the contents of crude protein (from 7.9 to 10.8%), ether extract (from 4.6 to 6.0%), nitrogen free extract (from 45.1 to 46.5%), and TDN (from 54.2 to 60.8%) were gradually increased, respectively. On the other hand, those of crude fiber (from 28.9 to 25.6%) and crud ash (from 13.5 to 11.2%) were decreased. These results showed that April 6 was the optimum seeding date with the sole object of feed production of Jeju barnyard grass under the environmental condition like as atmospheric phenomena and soil in Jeju region. (Key words : Jeju barnyard grass, Seeding date, Forage yield, Chemical composition)

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Effect of Organic Matter Ratios in Substrate and Mulching Materials on Growth of Liatris spicata under Non-irrigated Green Roofs (무관수 옥상녹화에서 유기질 비료와 멀칭재에 따른 리아트리스(Liatris spicata) 생육 반응)

  • Ju, Jin-Hee;Yoon, Yong-Han
    • Journal of the Korean Institute of Landscape Architecture
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    • v.40 no.2
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    • pp.130-137
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    • 2012
  • This research evaluated the effectiveness of organic matter ratios in substrate and mulching materials under mn-irrigated condition green roofs by measuring the effects on growth of Liatris spicata. Four mulching materials were installed, i. e. nun-mulched control(CON), volcanic ash soils(VAS), non-woven black fabric mat(NBM) and pine bark(PAK). Three levels of organic maller volume percentage in an amended soil were evaluated, amended soil: organic matter=100:0($A_1O_0$), amended soil: organic matter=80:20($A_4O_1$) and amended soil: organic matter=50:50($A_1O_1$). Plant height, number of leaves, diameter of flower stalk, number of florets, chlorophyll contents, shoot fresh and dry weight were recorded from April to September, 2010, and survival rate was examined on May 2011 of the following year. In the $A_1O_0$, the number of leaves, number of florets and chlorophyll contents were higher in Liatris spicata grown on NBM than other mulching treatments. Especially, plant height, shoot fresh and dry weight were significantly higher. However, it resulted the lowest survival rate than other mulching treatments. 2. In the $A_4O_1$, the plant height, number of leaves, number of florets, shoot fresh and dry weight were higher than other mulching treatments, but there was no significant difference except for the plant height of Liatris spicata grown on NBM. The survival rate was decreased by 40~60%, compared with $A_1O_0$, after overwintering. 3. In the $A_1O_1$, the plant height, number of leaves, diameter of flower stalk, number of florets, chlorophyll contents, shoot fresh and dry weight were slightly higher than other mulching treatments, but there was no significant difference from Liatris spicata grown on NBM and VAS. The survival rate was observed by 0% over all mulching treatments after overwintering. Therefore, the non-woven black fabric mat(NBM) promoted the Liatris spicata's growth and flowering compared with other mulching treatments. However, the survival rate was decreased significantly, and the organic matter ratios were increased after overwintering under non-irrigated green roofs.