• Title/Summary/Keyword: Frequency tuning

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Mutation Analysis of Synthetic DNA Barcodes in a Fission Yeast Gene Deletion Library by Sanger Sequencing

  • Lee, Minho;Choi, Shin-Jung;Han, Sangjo;Nam, Miyoung;Kim, Dongsup;Kim, Dong-Uk;Hoe, Kwang-Lae
    • Genomics & Informatics
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    • v.16 no.2
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    • pp.22-29
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    • 2018
  • Incorporation of unique barcodes into fission yeast gene deletion collections has enabled the identification of gene functions by growth fitness analysis. For fine tuning, it is important to examine barcode sequences, because mutations arise during strain construction. Out of 8,708 barcodes (4,354 strains) covering 88.5% of all 4,919 open reading frames, 7,734 barcodes (88.8%) were validated as high-fidelity to be inserted at the correct positions by Sanger sequencing. Sequence examination of the 7,734 high-fidelity barcodes revealed that 1,039 barcodes (13.4%) deviated from the original design. In total, 1,284 mutations (mutation rate of 16.6%) exist within the 1,039 mutated barcodes, which is comparable to budding yeast (18%). When the type of mutation was considered, substitutions accounted for 845 mutations (10.9%), deletions accounted for 319 mutations (4.1%), and insertions accounted for 121 mutations (1.6%). Peculiarly, the frequency of substitutions (67.6%) was unexpectedly higher than in budding yeast (~28%) and well above the predicted error of Sanger sequencing (~2%), which might have arisen during the solid-phase oligonucleotide synthesis and PCR amplification of the barcodes during strain construction. When the mutation rate was analyzed by position within 20-mer barcodes using the 1,284 mutations from the 7,734 sequenced barcodes, there was no significant difference between up-tags and down-tags at a given position. The mutation frequency at a given position was similar at most positions, ranging from 0.4% (32/7,734) to 1.1% (82/7,734), except at position 1, which was highest (3.1%), as in budding yeast. Together, well-defined barcode sequences, combined with the next-generation sequencing platform, promise to make the fission yeast gene deletion library a powerful tool for understanding gene function.

Analysis and Application of Compact Planar Multi-Loop Self-Resonant Coil of High Quality Factor with Coaxial Cross Section (고품질 계수를 갖는 소형 평판형 동축 단면 다중 루프 자기 공진 코일 해석 및 응용)

  • Son, Hyeon-Chang;Kim, Jinwook;Kim, Do-Hyeon;Kim, Kwan-Ho;Park, Young-Jin
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.24 no.4
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    • pp.466-473
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    • 2013
  • In this paper, a compact planar multi-loop self-resonant coil of high quality factor with a coaxial cross section is proposed for effective wireless charging. The proposed coil has high Q-factor and a resonant frequency of a coil can be easily controlled by adjusting distributed capacitance. For designing the coil, a self-inductance and a distributed capacitance are calculated theoretically. The self-inductance is calculated from the sum of the mutual energies between small circular loops that are made by dividing the cross section of the coil. To verify its properties and calculation results, the self-resonant coils are fabricated by using a coaxial cable with characteristic impedance of $50{\Omega}$. The measured frequencies are very consistent with the calculated ones. In addition, the resonant frequency can be adjusted slightly by the tuning parameter ${\gamma}$. The resonant coils are applied to a tablet PC, the Q-factors of the Tx and Rx resonant coils are 282 and 135, respectively. As a result of measurement when height between the two resonant coils is 4.4 cm, the power transfer efficiency is more than 80 % within a radius of 5 cm.

Development of Ultrasonic Waveguide Sensor for Under=Sodium Viewing in Liquid Metal Reactor (액체금속로 소듐내부 가시화를 위한 초음파 웨이브가이드 센서 개발)

  • Joo, Young-Sang;Lee, Jae-Han
    • Journal of the Korean Society for Nondestructive Testing
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    • v.26 no.1
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    • pp.18-24
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    • 2006
  • Reactor core and internal structures of a liquid metal reactor (LMR) can not be visually examined due to an opaque liquid sodium. The under-sodium viewing technique by using an ultrasonic wave should be applied far the visual inspection of reactor internals. In this study, an ultrasonic waveguide sensor with a strip plate has been developed for an application to the under-sodium viewing technique. The Lamb wave propagation of a waveguide sensor has been analyzed and the zero-order antisymmetric $A_0$ plate wave was selected as the application mode of the sensor. The $A_0$ plate wave can be propagated in the dispersive low frequency range by using a liquid wedge clamped to the waveguide. A new technique is presented which is capable of steering the radiation beam angle of a waveguide sensor without a mechanical movement of the sensor assembly The steering function of the ultrasonic radiation beam can be achieved by a frequency tuning method of the excitation pulse in the dispersive range of the $A_0$ mode. The technique provides an opportunity to overcome the scanning limitation of a waveguide sensor. The beam steering function has been evaluated by an experimental verification. The ultrasonic C-scanning experiments are performed in water and the feasibility of the ultrasonic waveguide sensor has been verified.

A Class-C type Wideband Current-Reuse VCO With 2-Step Auto Amplitude Calibration(AAC) Loop (2 단계 자동 진폭 캘리브레이션 기법을 적용한 넓은 튜닝 범위를 갖는 클래스-C 타입 전류 재사용 전압제어발진기 설계)

  • Kim, Dongyoung;Choi, Jinwook;Lee, Dongsoo;Lee, Kang-Yoon
    • Journal of the Institute of Electronics and Information Engineers
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    • v.51 no.11
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    • pp.94-100
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    • 2014
  • In this paper, a design of low power Current-Reuse Voltage Controlled Oscillator (VCO) which has wide tuning range about 1.95 GHz ~ 3.15 GHz is presented. Class-C type is applied to improve phase noise and 2-Step Auto Amplitude Calibration (AAC) is used for minimizing the imbalance of differential VCO output voltage which is main issue of Current-Reuse VCO. The mismatch of differential VCO output voltage is presented about 1.5mV ~ 4.5mV. This mismatch is within 0.6 % compared with VCO output voltage. Proposed Current-Reuse VCO is designed using CMOS $0.13{\mu}m$ process. Supply voltage is 1.2 V and current consumption is 2.6 mA at center frequency. The phase noise is -116.267 dBc/Hz at 2.3GHz VCO frequency at 1MHz offset. The layout size is $720{\times}580{\mu}m^2$.

Analysis of the Abstract Structure in Scientific Papers by Gifted Students and Exploring the Possibilities of Artificial Intelligence Applied to the Educational Setting (과학 영재의 논문 초록 구조 분석 및 이에 대한 인공지능의 활용 가능성 탐색)

  • Bongwoo Lee;Hunkoog Jho
    • Journal of The Korean Association For Science Education
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    • v.43 no.6
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    • pp.573-582
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    • 2023
  • This study aimed to explore the potential use of artificial intelligence in science education for gifted students by analyzing the structure of abstracts written by students at a gifted science academy and comparing the performance of various elements extracted using AI. The study involved an analysis of 263 graduation theses from S Science High School over five years (2017-2021), focusing on the frequency and types of background, objectives, methods, results, and discussions included in their abstracts. This was followed by an evaluation of their accuracy using AI classification methods with fine-tuning and prompts. The results revealed that the frequency of elements in the abstracts written by gifted students followed the order of objectives, methods, results, background, and discussions. However, only 57.4% of the abstracts contained all the essential elements, such as objectives, methods, and results. Among these elements, fine-tuned AI classification showed the highest accuracy, with background, objectives, and results demonstrating relatively high performance, while methods and discussions were often inaccurately classified. These findings suggest the need for a more effective use of AI, through providing a better distribution of elements or appropriate datasets for training. Educational implications of these findings were also discussed.

Detection of the gas-saturated zone by spectral decomposition using Wigner-Ville distribution for a thin layer reservoir (얇은 저류층 내에서 WVD 빛띠 분해에 의한 가스 포화 구역 탐지)

  • Shin, Sung-Il;Byun, Joong-Moo
    • Geophysics and Geophysical Exploration
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    • v.15 no.1
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    • pp.39-46
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    • 2012
  • Recently, stratigraphic reservoirs are getting more attention than structural reservoirs which have mostly developed. However, recognizing stratigraphic thin gas reservoirs in a stacked section is usually difficult because of tuning effects. Moreover, if the reflections from the brine-saturated region of a thin layer have the same polarity with those from the gas-saturated region, we could not easily identify the gas reservoir with conventional data processing technique. In this study, we introduced a way to delineate the gas-saturated region in a thin layer reservoir using a spectral decomposition method. First of all, amplitude spectrum with the variation of the frequency and the incident angle was investigated for the medium which represents property of Class 3, Class 1 or Class 4 AVO response. The results show that the maximum difference in the amplitude spectra between brine and gas-saturated thin layers occurs around the peak frequency independent of the incident angle and the type of AVO responses. In addition, the amplitude spectra of the gas-saturated zone are greater than those of brine-saturated one in Class 3 and Class 4 at the peak frequency while those of phenomenon occur oppositely in Class 1. Based on the results, we applied spectral decomposition method to the stacked section in order to distinguish the gas-saturated zone from the brine-saturated zone in a thin layer reservoir. To verify our new method, we constructed a thin-layer velocity model which contains both gas and brine-saturated zones which have the same reflection polarities. As a result, in the spectral decomposed sections near the peak frequency obtained by Wigner-Ville Distribution (WVD), we could identify the difference between reflections from gas- and brinesaturated region in the thin layer reservoir, which was hardly distinguishable in the stacked section.

Novel Power Bus Design Method for High-Speed Digital Boards (고속 디지털 보드를 위한 새로운 전압 버스 설계 방법)

  • Wee, Jae-Kyung
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.43 no.12 s.354
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    • pp.23-32
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    • 2006
  • Fast and accurate power bus design (FAPUD) method for multi-layers high-speed digital boards is devised for the power supply network design tool for accurate and precise high speed board. FAPUD is constructed, based on two main algorithms of the PBEC (Path Based Equivalent Circuit) model and the network synthesis method. The PBEC model exploits simple arithmetic expressions of the lumped 1-D circuit model from the electrical parameters of a 2-D power distribution network. The circuit level design based on PBEC is carried with the proposed regional approach. The circuit level design directly calculates and determines the size of on-chip decoupling capacitors, the size and the location of off-chip decoupling capacitors, and the effective inductances of the package power bus. As a design output, a lumped circuit model and a pre-layout of the power bus including a whole decoupling capacitors are obtained after processing FAPUD. In the tuning procedure, the board re-optimization considering simultaneous switching noise (SSN) added by I/O switching can be carried out because the I/O switching effect on a power supply noise can be estimated over the operation frequency range with the lumped circuit model. Furthermore, if a design changes or needs to be tuned, FAPUD can modify design by replacing decoupling capacitors without consuming other design resources. Finally, FAPUD is accurate compared with conventional PEEC-based design tools, and its design time is 10 times faster than that of conventional PEEC-based design tools.

A Pipelined Hash Join Method for Load Balancing (부하 균형 유지를 고려한 파이프라인 해시 조인 방법)

  • Moon, Jin-Gue;Park, No-Sang;Kim, Pyeong-Jung;Jin, Seong-Il
    • The KIPS Transactions:PartD
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    • v.9D no.5
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    • pp.755-768
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    • 2002
  • We investigate the effect of the data skew of join attributes on the performance of a pipelined multi-way hash join method, and propose two new hash join methods with load balancing capabilities. The first proposed method allocates buckets statically by round-robin fashion, and the second one allocates buckets adaptively via a frequency distribution. Using hash-based joins, multiple joins can be pipelined so that the early results from a join, before the whole join is completed, are sent to the next join processing without staying on disks. Unless the pipelining execution of multiple hash joins includes some load balancing mechanisms, the skew effect can severely deteriorate system performance. In this paper, we derive an execution model of the pipeline segment and a cost model, and develop a simulator for the study. As shown by our simulation with a wide range of parameters, join selectivities and sizes of relations deteriorate the system performance as the degree of data skew is larger. But the proposed method using a large number of buckets and a tuning technique can offer substantial robustness against a wide range of skew conditions.

Concentration of Sodium Chloride Solutions Sensing by Using a Near-Field Microwave Microprobe (비접촉 근접장 마이크로파 현미경을 이용한 NaCl 용액의 농도 측정)

  • Kim, Song-Hui;Yoon, Young-Woon;Babajanyan, Arsen;Kim, Jong-Chul;Lee, Kie-Jin
    • Journal of the Korean Society for Nondestructive Testing
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    • v.27 no.1
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    • pp.23-30
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    • 2007
  • We observed the NaCl concentration of solutions using a near-field microwave microprobe(NFMM). Instead of the usual technique, we take advantage of the noncontact evaluation capabilities of a NFMM. A NFMM with a high Q dielectric resonator allows observation of small variations of the permittivity due to changes in the NaCl concentration. The changes of NaCl concentration due to a change of permittivity of the NaCl solution were investigated by measuring the microwave reflection coefficient $S_{11}$ of the resonator. The NaCl sensor consisted of a dielectric resonator coupled to a probe tip at an operating frequency of about f=4 GHz. The change of the NaCl concentration is directly related to the change of the reflection coefficient due to a near field electromagnetic interaction between the probe tip and the NaCl solution. In order to determine the probe selectivity, we measured a mixture solution of NaCl and glucose.

Speed Sensorless Vector Control of Wound Induction Motor Using a MRAS Method (MRAS 기법을 이용한 권선형 유도전동기의 속도센서리스 벡터제어)

  • Choi, Hyun-Sik;Lee, Jae-Hak;Um, Tae-Wook
    • Journal of the Institute of Electronics Engineers of Korea TE
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    • v.42 no.1
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    • pp.29-34
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    • 2005
  • The wound induction motor can provide high starting torque and reduced starting current simultaneously by inserting large resistor externally when starting. And this technique is one of the well known methods among the induction motor starting methods and generally used for heavy load starting such as crane and cement factories. The conventional PI controller has been widely used in industrial application due to the simple control algorithm and is generally used for control of current torque, position, and speed for the wound induction motor drive system. However, the conventional control system for wound induction motor may result in poor performance because sensors have to be used but are often limited by the environmental condition. Recently, to overcome these problems, many sensorless vector control methods for the wound induction motor have been studied. This paper presents a MRAS method for sensorless vector control of the wound induction motor drive. In the conventional MRAS method, in low frequency, the stator resistance variation may result in poor performance. Therefore, this paper presents a MRAS method with stator and rotor resistance tuning for sensorless vector control of the wound induction motor to overcome several shortages of the conventional MRAS caused by parameter variation and to enhance the robustness of the sensorless vector control. The validity and effectiveness of the proposed method is verified through digital simulation.