• Clinical and Experimental Reproductive Medicine
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• 제34권2호
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• pp.117-124
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• 2007

목 적: 본 연구는 생쥐 전핵시기 배아를 완만동결법과 유리화동결법으로 동결-융해 후 배아의 생존율과 성장률을 비교하고자 시행하였다. 연구방법: 과배란을 유도한 생쥐로부터 전핵시기 배아를 획득하여 10% SSS가 첨가된 HTF 배양액으로 약 1시간 동안 배양한 후 두 개의 전핵이 관찰되는 정상적인 형태의 배아만 선별하여 동결하였다. 동결방법으로는 1.5 M PROH에 0.1 M sucrose가 함유된 완만동결법과 40% ethylene glycol, 18% Ficoll, 0.5 M sucrose가 혼합된 EFS40 용액과 EM grid를 이용하여 이용한 유리화동결법을 실시하였다. 동결-융해 후 전핵시기 배아의 회수율, 생존을 및 부화 포배기로의 성장률과 부화율을 비교하였다. 결 과: 각각의 방법으로 동결-융해 후 24시간 동안 배양하였을 때 2-세포기까지의 성장률은 완만동결군이 59.1%이었고 유리화동결군이 77.0%로 두 군간에 유의한 차이를 보였고 (p<0.003), 48시간 동안의 배양에서도 완만동결군이 53.3%이고 유리동결군이 72.6%로 유의하게 유리화동결군에서 높은 수세포기까지의 성장률을 보였으며 (p<0.003), 72시간 배양하였을 때의 상실배로의 성장률 역시 완만동결군이 46.7%이고 유리화동결군이 67.3%로 유리화동결군에서 유의하게 높은 성장률을 보였다 (p<0.001). 융해 후 144시간 동안 배양하였을 때의 부화포배기로의 성장률은 완만동결군이 26.3%이고 유리화동결군이 43.4%로 유리화동결군에서 유의하게 높은 성장률을 보였다 (p<0.005). 결 론: 생쥐 전핵시기 배아의 동결보존에서 유리화동결법은 완만동결법 보다 시간이 단축되고 비싼 장비가 필요없어 경제적이고 간단했을 뿐 아니라 동결-응해 후 전반적으로 높은 생존율과 성장률을 나타내었다.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2003년도 가을 학술발표회 논문집
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• pp.48-53
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• 2003

The steel slag, a by-product which is produced on the process of manufacturing steel by refining pig iron is mainly used as road materials after aging it. It is necessary to age steel slag for long time in air because the reaction with water and free-CaO in steel slag could make the volume expanded. Due to this reason it prevents steel slag from being used as aggregate of concrete. But steel slag used in this study is controled by a air-jet method which rapidly cools substance melted at a high temperature. Rapid cooling prevents from generating of free-CaO in steel slag. In this study, it was investigated that steel slag manufactured by air-jet method affects on concrete in the freezing and thawing. As results of this study, concrete mixed with steel slag was worse in the freezing and thawing than concrete mixed with sand in spite of using air entraining agent. To obtain durability of concrete in the freezing and thawing, it is desirable to mix 50% of steel slag in concrete per unit weight of volume.

• Nuclear Engineering and Technology
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• 제34권2호
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• pp.105-116
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• 2002

In special industrial fields such 3s nuclear power plants and chemical plants, it is often necessary to repair system components without plant shutdown or drainage of system having many piping structures which may have hazardous or expensive fluid. A temporary ice plugging method for blocking internal flow is considered as a useful method in that case. According to the pipe freezing guideline of the nuclear power plant, the length of a freezing jacket must be longer than twice of the pipe diameter. However, for applying the ice plugging to short pipes which do not have enough freezing length because of geometrical configuration, it is inevitable to use shorter jacket less than twice of the pipe diameter. In this study, the integrity evaluation for short pipes in the nuclear power plant Is conducted by an experiment and the finite element analysis. From the results, the ice plugging process in short pipes can be safely carried out without any plastic deformation and fracture.

• 한국식품과학회지
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• 제32권2호
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• pp.341-348
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• 2000

기존 냉장고의 특선실과 새로운 냉각방식의 냉장고에 돼지고기, 쇠고기, 냉동참치를 저장하면서 그들의 품질을 비교하여 각 냉각방식의 차이가 부분 냉동식품의 품질에 미치는 영향을 조사하였다. 일반 냉장고 특선실$(-1^{\circ}C)$, 강제순환식 저장방법$(-5^{\circ}C)$, 그리고 자연대류식 저장방법$(-5^{\circ}C)$으로 저장하면서, 돼지고기와 쇠고기는 VBN, TBA, 및 수분함량을, 냉동참치는 K-value와 VBN값의 변화를 측정하였다. 저장 기간 중 모든 시료에 대해서 강제순환식 저장방법에서 수분함량이 급격히 감소하였으며 VBN의 경우도 수분함량에 영향을 받아서 강제순환방식이 다른 저장방법들보다 높은 수치를 보였다. 돼지고기의 경우 TBA값에 대한 유의적인 차이는 없었으며 쇠고기의 경우는 VBN에 대하여 유의적 차이는 나타나지 않았으나 TBA값은 일반 특선실과 자연대류식 저장방법에 대하여 유의적 차이를 보였다. 반면 포장을 한 경우는 일반 특선실과 강제순환 및 자연대류 저장방법에서 돼지고기의 품질의 차이가 발생하였다. 냉동참치는 VBN과 K-value에 대하여 일반 특선실과 자연대류식에 유의적인 차이가 있었다. 따라서 cold chamber의 온도를 $-5^{\circ}C$ 정도인 subfreezing온도로 유지하고, 자연대류 냉각방식을 적용하면 가정용 냉장고를 사용하여 육류 및 어류의 신선도를 현저히 연장시킬 수 있을 것으로 보인다.

• 한국수산과학회지
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• 제44권6호
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• pp.665-670
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• 2011

The rotifer Brachionus plicatilis is one of the most important food organisms in aquaculture. The resting eggs produced by mictic female rotifers are easily stored and hatched, making them useful as the starter for the mass culture of rotifers in marine larval culture. This study examined the optimum preservation method for resting eggs to ensure a high hatching rate. To produce resting eggs, the marine rotifer B. plicatilis was cultured with Nannochloris oculata (KMMCC 16). The resting eggs were harvested and cryopreserved using 5% and 10% methanol (MeOH), dimethylsulfoxide (DMSO), and glycerol as cryoprotectant agents (CPAs). The cryopreservation comprised slow or rapid freezing and the resting eggs were stored for one month in liquid nitrogen ($-196^{\circ}C$). The resting eggs were also dried at different temperatures (30, 40, and $50^{\circ}C$) and for different times (1, 2, and 3 h). In general, the hatching rates of the resting eggs preserved with CPA were higher than those without CPA and the slow freezing method was better than the rapid freezing method. However, the optimum CPA concentration for the hatching rate of the resting eggs varied with the freezing method and kind of CPA, and the CPA also affected the viability of the resting eggs. Dried resting eggs had a high, rapid hatching rate over 80%. The moisture content of the resting eggs cryopreserved in liquid nitrogen affected the hatching rate. Drying at $30^{\circ}C$ for 1 hour resulted in a high hatching rate of the resting eggs. In conclusion, drying at $30^{\circ}C$ for 1 hour and preservation in liquid nitrogen with the slow freezing method, without CPA, is recommended for a high hatching rate (ca. 95%) of rotifer resting eggs.

• 대한수의학회지
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• 제33권1호
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• pp.171-178
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• 1993

The present study was carried out to investigate the developmental potency to blastocyst after freezing and thawing of nuclear transplanted 2-cell embryos. The nuclei from 2-, 4- and 8-cell mouse embryos were transferred into enucleated 2-cell embryos, and the reconstituted embryos were submitted to direct current(DC) pulse at output voltage of 2.0 kV/cm for $100{\mu}$ sec to induce cell fusion. The recovery rate and developmental potency to blastocyst after freezing and thawing of nuclear transplanted 2-cell embryos was investigated. 1. The recovery rate of nuclear transplanted 2-cell embryos in normal morphology after freezing and thawing was significantly higher in rapid freezing(DMSO 4.5M) than in slow cooling(p<0.01). 2. When the recovered embryos in normal morphology were cultured in vitro, there were no significant differences in the developmental potency to blastocyst between the freezing methods and the concentrations of cryoprotectant. In summary, these experiments have proved that rapid freezing method(DMSO 4.5M) is effective in nuclear transplanted 2-cell mouse embryos. If improved micromanipulation techniques and freezing are combined, nuclear transplantation technique will contribute to the improvement of productivity in livestock animals.

• 설비공학논문집
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• 제19권11호
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• pp.782-788
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• 2007

In this paper, the freezing process of the water supply pipe in the exterior wall of an apartment house was analyzed by numerical method. The thickness of the pipe insulation and the percentage of insulation damage were considered as parameters in this paper. In the cases of the 0%, 8% and 20% damaged of the 5mm thickness insulation, the freezing was completed after 13 hours, 10 hours and 7 hours respectively. And in cases of the 10mm thickness insulation, the freezing was completed after 18 hours, 10.5 hours and 8 hours respectively. As a result, it is predicted that the water freezing would occurred when the water supply pipe with 8% or 20% damaged insulation are installed in the exterior wall. However, the water freezing would not occurred when the water supply pipe with 10mm thickness insulation of 0% damage is installed in the exterior wall.

• Smart Structures and Systems
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• 제17권1호
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• pp.45-58
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• 2016

Freezing and thawing cycles induce deterioration and strength degradation of concrete structures. This study presumes that a large quantity of contact-type defects develop due to the freezing and thawing cycles of concrete and evaluates the degree of defects based on a nonlinearity parameter. The nonlinearity parameter was obtained by an impact-modulation technique, one of the nonlinear ultrasonic methods. It is then used as an indicator of the degree of contact-type defects. Five types of damaged samples were fabricated according to different freezing and thawing cycles, and the occurrence of opening or cracks on a micro-scale was visually verified via scanning electron microscopy. Dynamic modulus and wave velocity were also measured for a sensitivity comparison with the obtained nonlinearity parameter. The possibility of evaluating strength degradation was also investigated based on a simple correlation of the experimental results.

• 한국도로학회논문집
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• 제15권3호
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• pp.65-74
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• 2013

PURPOSES: This study is to evaluate the dynamic modulus changes of permeable asphalt mixtures by using non-destructive impact testing method and to compare the dynamic moduli of permeable asphalt mixtures through repeated freezing and thawing conditions. METHODS: For the study, non-destructive impact testing method is used in order to obtain dynamic modulus of asphalt specimen and to confirm the change of dynamic modulus before and after freezing and thawing conditions. RESULTS : This study has shown that the dynamic moduli of asphalt concrete specimens consisting of 10%, 15% and 20% porosity are reduced by 11.851%, 1.9564%, 24.593% after freezing and thawing cycles. CONCLUSIONS : Non-destructive impact testing method is very useful and has repeatability. Specimen with 15% porosity has high durability than others.

• Journal of Advanced Marine Engineering and Technology
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• 제20권3호
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• pp.162-170
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• 1996

A numerical study on the Stefan problem occurred in cryosurgery is performed. Crank-Nicholson type finite difference algorithm based on the enthaly method is adapted to solve the phase change problem in this study. As it is a moving boundary problem, special emphasis is put on the estimation of the freezing front location. Two cases selected here are freezings of human tissue by disk type cryoprobe and by hemispherical one. In both cases, the heat flows are considered to be one dimensional. The calculated results using enthalpy method are compared with those using the program TRUMP and with Neumann's solution. These results agree guite well with each other. While it is pretty difficult to get accurate freezing front location by TRUMP due to the so- called "phase change knee" occured during the phase change, the algorithm based on the enthalpy method is proved to be very powerful to cope with this kind of problem.f problem.