• Title/Summary/Keyword: Freezing Method

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Engineering Characteristics of Liquid Filler Using Marine Clay and In-situ Soil (해양점토와 현장토를 활용한 유동성 채움재의 공학적 특성)

  • Oh, Sewook;Bang, Seongtaek
    • Journal of the Korean GEO-environmental Society
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    • v.21 no.9
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    • pp.25-32
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    • 2020
  • The underground utilities installed under the ground is an important civil engineering structure, such as water supply and sewerage pipes, underground power lines, various communication lines, and city gas pipes. Such underground utilities can be exposed to risk due to external factors such as concentrated rainfall and vehicle load, and it is important to select and construct an appropriate backfill material. Currently, a method mainly used is to fill the soil around the underground utilities and compact it. But it is difficult to compact the lower part of the buried pipe and the compaction efficiency decreases, reducing the stability of the underground utilities and causing various damages. In addition, there are disadvantages such as a decrease in ground strength due to disturbance of the ground, a complicated construction process, and construction costs increase because the construction period becomes longer, and civil complaints due to traffic restrictions. One way to solve this problem is to use a liquid filler. The liquid filler has advantages such as self-leveling ability, self-compaction, fluidity, artificial strength control, and low strength that can be re-excavated for maintenance. In this study, uniaxial compression strength test and fluidity test were performed to characterize the mixed soil using marine clay, stabilizer, and in-situ soil as backfill material. A freezing-thawing test was performed to understand the strength characteristics of the liquid filler by freezing, and in order to examine the effect of the filling materials on the corrosion of the underground pipe, an electrical resistivity test and a pH test were performed.

Studies on Preservation of Concentrated Milk by Freeze - Flow Process (Freeze - Flow Process 를 이용한 농축우유의 저장에 관한 연구)

  • Lee, Young-Chun;Shin, Dong-Bin
    • Korean Journal of Food Science and Technology
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    • v.17 no.6
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    • pp.500-505
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    • 1985
  • A method to store concentrated milk in the liquid state at $-15^{\circ}C$ was developed, and quality changes during storage of milk were evaluated. Combined cryoprotectants (CCP) suitable for storing concentrated milk in the liquid state at $-15^{\circ}C$ were consisted of 17.74% sucrose, 8.87% glucose, 8.87% fructose, 2% glycerol, 0.25% sodium hexametaphosphate, 0.25% NaCl and 0.02% ascorbic acid. The amount of CCP to be added to concentrated milk to depress freezing point to $-15^{\circ}C$ was 38% by weight. Gelation due to protein denaturation was the most serious quality change during storage, which adversely affected appearance and utilization of the stored product. Gelation was observed after 3 weeks storage in the control, but it was not in milk with CCP throughout 18 weeks storage. Amount of protein precipitated increased in the control during storage, whereas there was no protein precipitated in milk with CCP. Surface color and peroxide value of the control and treatment did not change significantly during storage, and there were no marked differences between the control and treatment. These results indicated that quality of concentrated milk could be preserved, without gelation, by storing milk with CCP in the -liquid state at the frozen storage temperature. Besides, energy required for freezing preservation of milk could be significantly reduced by elimination of phase changes for freezing and thawing, and the stored product could be continuously processed for the final products without long waiting time for thawing.

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Variation of Electrical Resistivity Characteristics in Sand-Silt Mixtures due to Temperature Change (온도변화에 따른 모래-실트 혼합토의 전기비저항 특성변화)

  • Park, Jung-Hee;Seo, Sun-Young;Hong, Seung-Seo;Kim, YoungSeok;Lee, Jong-Sub
    • Journal of the Korean GEO-environmental Society
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    • v.13 no.10
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    • pp.25-32
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    • 2012
  • The application of electrical resistivity, which is related to charge mobility, has increased in the field of geotechnical engineering for the detection of underground cavern, faults and subsurface pollution level. The purpose of this study is to investigate the variation of electrical resistivity due to temperature change. Sand-silt mixture specimens prepared in the square freezing nylon cell are frozen in the frozen chamber. Four electrodes are attached on the four side walls of the freezing cell for the measurement of electrical resistance during temperature change. Electrical resistances of sand-silt mixtures with different degrees of saturation (0%, 2.5%, 5%, 10%, 20%, 40%, 60% and 100%) are measured as the temperature of specimens decrease from $20^{\circ}C$ to $-10^{\circ}C$. The electrical resistances determined by Ohm's law are transformed into the electrical resistivity by calibration. Experimental results show that the higher degree of saturation, the lower electrical resistivity at $20^{\circ}C$. Electrical resistivity gradually increases as the temperature decrease from $20^{\circ}C$ to $0^{\circ}C$. For the specimens with the degree of saturation of 15% or higer, electrical resistivity dramatically changes near the temperature of $0^{\circ}C$. In addition, very high electrical resistivity is observed regardless of the degree of saturation if the specimens are frozen. This study provides the fundamental information of electrical resistivity according to the soil freezing and temperature change demonstrates that electrical resistivity be a practical method for frozen soil investigation.

The Cytoskeletal and Chromosomal Constitution of Vitrified Immature Mouse Oocytes (초자화동결된 생쥐 미성숙란의 세포골격과 염색체성상)

  • Park, Se-Pill;Yi, Bong-Kyung;Kim, Eun-Young;Nam, Hwa-Kyung;Lee, Keum-Sil;Yoon, San-Hyun;Chun, Kil-Saeng;Lim, Jin-Ho
    • Clinical and Experimental Reproductive Medicine
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    • v.26 no.3
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    • pp.363-368
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    • 1999
  • This study was to confirm whether the vitrification method using EFS40 freezing solution has detrimental effect on the cytoskeleton and chromosome constitution of the immature mouse oocytes by indirect immunocytochemistry and chromosome analysis. Immature mouse oocytes were vitrified using EFS40 (40% EG, 18% ficoll, 0.5 M sucrose diluted in M2 medium), thawed and then survived oocytes were in vitro matured for 16 hr. When the microtubule morphology and micro filament distribution in vitrified-thawed immature mouse oocytes were examined, normal percentage of two cytoskeleton in vitrified group (93.9 and 100.0%) was not significantly different from that in control (100.0 and 100.0%) and exposed group (94.4 and 100.0%). The rate of oocytes containing a normal chromosome number in vitrified group was 65.8%, this result was not significantly different from that in control (79.6%) and exposed group (69.0%). These results indicated that exposure to cryoprotectant or freezing has not effect on the alteration of cytoskeleton morphology and the chromosome constitution of mouse oocytes and that our vitrification methods using EFS40 freezing solution was suitable for the cryopreservation of immature mouse oocytes.

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Effects of Freezing of Gait on Spatiotemporal Variables, Ground Reaction Forces, and Joint Moments during Sit-to-walk Task in Parkinson's Disease

  • Park, Hwayoung;Youm, Changhong;Son, Minji;Lee, Meounggon;Kim, Jinhee
    • Korean Journal of Applied Biomechanics
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    • v.28 no.1
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    • pp.19-27
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    • 2018
  • Objective: This study aimed to analyze the effects of freezing of gait on spatiotemporal variables, ground reaction forces (GRFs), and joint moments during the sit-to-walk task at the preferred and maximum speeds in patients with Parkinson's disease (PD). Method: The subjects were classified by a neurologist into 12 freezers, 12 non-freezers, and 12 controls. Sit-to-walk parameters were measured during three repetitions of the task in a random order at the preferred and maximum possible speeds. Results: In the sit-to-walk task at the preferred speed, the freezers and non-freezers exhibited a higher peak anterior-posterior GRF (p<0.001) in the sit-to-stand phase and lower step velocity (p<0.001), step length (p<0.001), and peak anterior-posterior GRF (p<0.001) in the first-step phase than the controls. The freezers had higher peak anterior-posterior GRF (p<0.001) and peak moment of the hip joint (p=0.008) in the sit-to-stand phase than the non-freezers. In the sit-to-walk phase at the maximum speed, the freezers and non-freezers had lower peak moment of the hip joint (p=0.008) in the sit-to-stand phase than the controls. The freezers and non-freezers displayed lower step velocity (p<0.001) and peak anterior-posterior GRF (p<0.001) in the first-step phase than the controls. The freezers showed higher peak moments of the hip joint in the sit-to-stand phase than the non-freezers (p=0.008). Conclusion: The PD patients had reduced control ability in sit-to-stand motions for efficient performance of the sit-to-walk task and reduced performance in the sit-to-walk task. Furthermore, the freezers displayed reduced control ability in the sit-to-stand task. Finally, the PD patients exhibited a lower ability to control dynamic stability with changes in speed than the controls.

Analysis on Adequacy of the Satellite Soil Moisture Data (AMSR2, ASCAT, and ESACCI) in Korean Peninsula: With Classification of Freezing and Melting Periods (인공위성 기반 토양 수분 자료들(AMSR2, ASCAT, and ESACCI)의 한반도 적절성 분석: 동결과 융해 기간을 구분하여)

  • Baik, Jongjin;Cho, Seongkeun;Lee, Seulchan;Choi, Minha
    • Korean Journal of Remote Sensing
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    • v.35 no.5_1
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    • pp.625-636
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    • 2019
  • Soil moisture is a representative factor that plays a key role in hydrological cycle. It is involved in the interaction between atmosphere and land surface, and is used in fields such as agriculture and water resources. Advanced Microwave Scanning Radiometer 2 (AMSR2), Advanced SCATterometer (ASCAT), and European Space Agency Climate Change Initiative (ESACCI) data were used to analyze the applicability and uncertainty of satellite soil moisture product in the Korean peninsula. Cumulative distribution function (CDF) matching and triple collocation (TC) analysis were carried out to investigate uncertainty and correction of satellite soil moisture data. Comparisons of pre-calibration satellite soil moisture data with the Automated Agriculture Observing System (AAOS) indicated that ESACCI and ASCAT data reflect the trend of AAOS well. On the other hand, AMSR2 satellite data showed overestimated values during the freezing period. Correction of satellite soil moisture data using CDF matching improved the error and correlation compared to those before correction. Finally, uncertainty analysis of soil moisture was carried out using TC method. Clearly, the uncertainty of the satellite soil moisture, corrected by CDF matching, was diminished in both freezing and thawing periods. Overall, it is expected that using ASCAT and ESACCI rather than AMSR2 soil moisture data will give more accurate soil moisture information when correction is performed on the Korean peninsula.

320 Pesticides Analysis of Essential Oils by LC-MS/MS and GC-MS/MS (LC-MS/MS 와 GC-MS/MS 를 이용한 에센셜 오일 중 320 종 잔류농약 분석법 개발)

  • Oh, Ka Hyang;Park, Sung Mak;Lee, So Min;Jung, So Young;Kwak, Byeong-Mun;Lee, Mi-Gi;Lee, Mi Ae;Choi, Sung Min;Bin, Bum-Ho
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.47 no.4
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    • pp.317-331
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    • 2021
  • Essential oil is a volatile substance obtained by physically obtaining fragrant plant materials made by one single plant and plant species, and is widely used for cosmetics, fragrances, and aroma therapy due to its excellent preservation, sterilization, and antibacterial effects. When essential oil would undergo the extraction and concentration processes, the agricultural chemicals thereof would be extracted and concentrated only to be harmful to the human body. This study analyzes 320 residual agricultural chemicals concentrated in the essential oil, and to this end, LC-MS/MS and GC-MS/MS are used, while the freezing process is applied instead of the conventional refining process hexane, to improve the preprocessing method. As a result of analyzing the essential oil, such ingredients as chlorpyrifos, piperonyl butoxide and silafluofen have been detected in Basil oil and Clove leaf oil. Hence, it is perceived that the residual agricultural chemicals should continue to be monitored for the essential oil.

The Effects of Cryopreservation on Fine Structures of Pearl Oyster(Pinctada fucata martensii) Larvae (냉동보존이 진주조개(Pinctada fucata martensii) 유생의 미세구조에 미치는 영향)

  • Choi, Youn-Hee;Jo, Pil-Gue;Kim, Tae-Ik;Bai, Sung-Chul C.;Chang, Young-Jin
    • Development and Reproduction
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    • v.11 no.2
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    • pp.79-84
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    • 2007
  • The freezing susceptibilities of two larval stages (D-shaped and umbo) of the pearl oyster (Pinctada fucata martensii) were evaluated by the electron microscopy (light, transmission electron and scanning electron). The morphological shapes were examined from each pre-frozen or frozen-thawed stage of the cryopreserved larvae in liquid nitrogen by using the cryoprotectant, dimethyl sulfoxide ($Me_2SO$) mixed with sucrose. Although a portion of the shell was damaged, the hinge and prodissoconch were intact and clearly visible after preservation in liquid nitrogen. In addition, the cytoplasm of the frozen-thawed larvae maintained the normal organelle integrities, e.g., endoplasmic reticula, lipid droplets, mitochondria, nucleus and microvilli. However, some of the frozen-thawed larvae showed irregularly arranged cilia, rough shell surfaces and round-lumped cilium heads. These results indicate that P. fucata martensii larvae are susceptible to freezing, at least at those two critical developmental stages (D-shaped and umbo), and suggest a new industrial investigation including reduction method of cell injury for preserving microbial starter cultures need to be developed.

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Fundamental Study for Development of an Anti-Icing Pavement System Using Carbon-Fiber Sheet (탄소섬유 쉬트를 활용한 도로 결빙방지 시스템 개발을 위한 기초연구)

  • Lim, Chisu;Park, Kwangpil;Lee, Jaejun;Lee, Byungsuk
    • International Journal of Highway Engineering
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    • v.18 no.3
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    • pp.59-65
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    • 2016
  • PURPOSES : This paper aims to develop a road pavement de-icing system using carbon sheet to replace the older snow de-icing method. Carbon sheet is a light and high-strength metal. Hence, various bodies of research for its applications in many industries have progressed. METHODS : The experiment was conducted in a laboratory. The carbon sheet supplied voltage through a power supply system, and produced heat transfers to the concrete surface. Various factors, such as pavement material, carbon sheet width, penetration depth, and freezing-thawing resistance, were considered in the conducted experiments to confirm the heating transfer efficiency of the carbon sheet. RESULTS : The carbon sheet used was a conductor. Therefore, it produced heat if voltage was supplied. The exposed carbon sheet on the atmosphere did not affect the carbon sheet width when it provided constant voltage. However, the sheet showed different heating behaviors by width change when the carbon sheet penetrated into the concrete. Moreover, the freezing-thawing resistance was decreased by the carbon sheet with increasing width. CONCLUSIONS : The experiments confirmed the possibility of developing a road snow melting system using a carbon sheet. The antiicing system using the carbon sheet to replace the traditional anti-icing system has disadvantages of environmental pollution risk and electric leakage. The pavement also improved its toughness resistance. The utilization value will be very high in the future if carbon sheet heat loss can be minimized and durability is improved.

Performance Analysis of Freezing Desalination System using Seawater Heat Pump (해수 히트펌프를 이용한 냉동법 담수화시스템 개념설계)

  • Lee, Ho-Saeng;Lee, Seung-Won;Yoon, Jung-In;Kim, Hyeon-Ju
    • Journal of Advanced Marine Engineering and Technology
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    • v.35 no.4
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    • pp.373-378
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    • 2011
  • The freeze desalination cycle with seawater heat pump system is simulated and designed for the basic data for the design of freeze desalination system. The basic model of seawater heat pump system is refrigeration cycle and indirect freeze desalination method is used for seawater desalination. The cycle performance of seawater heat pump such as COP, compressor work, condensing capacity was analyzed and the desalination performance such as fresh water productivity and energy per unit fresh water productivity was compared with respect to the seawater temperature of condenser inlet and ice ratio in the evaporator. The compressor work and condensing capacity decreased with respect to the decrease of seawater inlet temperature. The energy per unit fresh water productivity in case of $8^{\circ}C$ seawater inlet temperature showed 28.9% lower than that of $20^{\circ}C$.