• BMB Reports
• /
• v.40 no.5
• /
• pp.684-689
• /
• 2007

The endogenous neurotoxin, 1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline (salsolinol), has been considered a potential causative factor for the pathogenesis of Parkinson's disease (PD). In the present study, we examined the pattern of human Cu,Zn-superoxide dismutase (SOD) modification elicited by salsolinol. When Cu,Zn-SOD was incubated with salsolinol, some protein fragmentation and some higher molecular weight aggregates were occurred. Salsolinol led to inactivation of Cu,Zn-SOD in a concentration-dependent manner. Free radical scavengers and catalase inhibited the salsolinol-mediated Cu,Zn-SOD modificaiton. Exposure of Cu,Zn-SOD to salsolinol led also to the generation of protein carbonyl compounds. The deoxyribose assay showed that hydroxyl radicals were generated during the oxidation of salsolinol in the presence of Cu,Zn-SOD. Therefore, the results indicate that free radical may play a role in the modification and inactivation of Cu,Zn-SOD by salsolinol.

• Language and Information
• /
• v.5 no.1
• /
• pp.35-53
• /
• 2001

As a subtype of English relative clause constructions, free relative constructions like what John ate in I ate what John ate exhibit complicated syntactic and semantic properties. In particular, the constructions have mixed properties of nominal and verbal: they have the internal syntax of sentence and the external syntax of noun phrase. This paper provides a constraint-based approach to these mixed constructions, and shows that simple constructional constraints are enough to capture their complexities. The paper begins by surveying the properties of the constructions. In discusses two types(Specific and nonspecific) of free relatives, their ,lexical restrictions nominal properties and behavior with respect to extraposition, piped piping and stacking Following these it sketches the basic framework of the HPSG(Head-driven Phrase Structure Grammar) which is of relevance in this paper. As the main part, the paper presents a constraint- based analysis in which tight interactions between grammatical constructions and a rich network of inheritance relations play important roles in accounting for the basic as well as complex properties of the constructions is question.

• Proceedings of the IEEK Conference
• /
• 2006.06a
• /
• pp.877-878
• /
• 2006

In this paper, we propose a skin lesion detection to develop the system of fluorescence image analysis to identify the fluorescence of topical methyl aminolevulinate(MAL) idduced PpIX in patients with BCC accurately. By fluorescence image analysis we define the border between tumo and tumor-free areas on fluorescence image after topical application of MAL ointment. We excised both the tumor and peri-tumoral areas widely from the 10 patients with BCC, and divided tissue samples into 3 area, such as tumor area, suspected tumor area, tumor-free area, respectively. Our proposed method migt play a role as an adjunctive tool to define the border between tumor and tumor-free areas for Mohs' micrographic surgery.

• The Journal of Korean Academy of Sensory Integration
• /
• v.4 no.1
• /
• pp.65-70
• /
• 2006

Objective : Korean Academy of Sensory Integration(KASI) planned and executed intensive sensory integration treatment course for children with the sensory integrative dysfunction and therapists, to suggest practical guideline for therapists through individual supervision, lecture, simulation therapy, observation, case study and free play etc. Method : The course was held during 5days in the children's center for developmental support that set up sensory integration tools. The course executed the 4 individual interventions, 1 simulation therapy, 1 observation, 4 free play sessions that consisted of 6 children with sensory integrative dysfunction and 6 therapists who manage them and 3 supervisors and 2 managers. Results : Their parents reported the satisfaction degree of the treatment course service was above 84%. Therapists also had a chance to understand the broader sensory integrative interventions through the supervision and various educational programs. Conclusions : Intensive treatment course can provide parents and therapists with satisfaction of the programs. Therapists can especially experience reeducation individually through supervision and lectures.

• Korean Journal of Child Studies
• /
• v.37 no.1
• /
• pp.49-60
• /
• 2016

Objective: The purpose of the study was to explore the relationship between the daily routines and physical environments of daycare centers. Methods: A total of 20 classes at 8 different daycare centers from the metropolitan city of Daejeon were investigated. Trained observers visited each daycare center more than 3 times and collected data about daily routines using the Observational Log for Daily Routines in Daycare Centers. Also, the physical environments of each class were investigated in terms of actual size, layout of activity center, etc. Results and Conclusion: Results of the study were: first, daily routines of daycare centers could be divided into 2 distinguishable types by cluster analysis- 'free play oriented' and 'group activity oriented' Second, the amount of space each child has in a classroom differed according to the total size of the classroom. An average of 7 activity centers existed in each classroom. Third, there was a difference between free play oriented and group activity oriented groups in terms of the classroom size allocated for each child and the organization of the activity centers.

• Korean Journal of Childcare and Education
• /
• v.9 no.1
• /
• pp.241-262
• /
• 2013

The purpose of this study is to explore four-year old children's peer culture. During free choice play time, various activities and interaction patterns were observed and analyzed to understand the characteristics of peer culture. The results of this study showed two main peer culture patterns. First of all, peaceful peer culture in which children maintained close relationships based on mutual respect were found. Also conflictive peer culture in which children had negative relationships with other children, such as vertical, discriminative and neglected relationships were observed.

• Journal of Korean Library and Information Science Society
• /
• v.48 no.4
• /
• pp.49-76
• /
• 2017

Free learning semester aims to foster key competencies in junior high students via reinforcing student participation and career education. This paper investigates the programs of school and public libraries, as well as the roles that university libraries and LIS could play in the changing educational environment. School libraries, though an essential element in the educational infrastructure, are seriously underused. In addition, the support programs of public libraries tend to focus on guest lectures or field trips, rather than reinforcing the students to experience the foundational library services. Public libraries are in a good position to play an active role in strengthening school libraries. They should take a proactive role in forming a partnership with university libraries, LIS faculty and students, and in developing diverse programs ranging from vocational/career education to enhancing key competencies.

• Journal of Nutrition and Health
• /
• v.10 no.4
• /
• pp.97-103
• /
• 1977

Changes of free amino acids as taste compounds during the fermentation of oyster were analyzed by amino acid autoanalyzer. In fresh oyster, taurine, glutamic acid and alanine were abundant amino acids and the amounts of taurine (731mg%, on moisture and salt free base), glutamic acid (365mg%) and alanine (345.4mg% ) were 63.8% of the total free amino acids. Cystine, isoleucine, phenylalanine, leucine and histidine were detected as less abundant free amino acids and the amount of those amino. acids ranged from 5. 5mg% (cystine) to 32.9mg% (histidine). The free amino acids analyzed in this experiment were not changed in composition hut changed in amounts during 124 days of fermentation. Aspartic acid and leucine were continually increased during 124 days of fermentation. Lysine, histidine, threonine, serine, glutamic acid, tyrosine and phenyalanine were increased unlit 68 days of fermentation and than decreased gradually. The increase of arginine, glycine, valine and isoleucine were fluctuated. Taurine were dramatically decreased during the 124 days of fermentation. It is believed that glutamic acid, alanine, lecuine, serine, Iysine and threonine play an important role as taste compounds in fermented oyster because those amino acids were most abundant in fermented oyster.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.10
• /
• pp.1621-1628
• /
• 2015

Chlamydia possesses a conserved 7.5 kb plasmid that is known to play an important role in chlamydial pathogenesis, since some chlamydial organisms lacking the plasmid are attenuated. The chlamydial transformation system developed recently required the use of plasmid-free organisms. Thus, the generation and identification of plasmid-free organisms represent a key step in understanding chlamydial pathogenic mechanisms. A tricolor immunofluorescence assay for simultaneously detecting the plasmid-encoded Pgp3 and whole organisms plus DNA staining was used to screen C. muridarum organisms selected with novobiocin. PCR was used to detect the plasmid genes. Next-generation sequencing was then used to sequence the genomes of plasmid-free C. muridarum candidates and the parental C. muridarum Nigg strain. We generated five independent clones of plasmid-free C. muridarum organisms by using a combination of novobiocin treatment and screening plaque-purified clones with anti-Pgp3 antibody. The clones were confirmed to lack plasmid genes by PCR analysis. No GlgA protein or glycogen accumulation was detected in cells infected with the plasmid-free clones. More importantly, whole-genome sequencing characterization of the plasmid-free C. muridarum organism and the parental C. muridarum Nigg strain revealed no additional mutations other than loss of the plasmid in the plasmid-free C. muridarum organism. Thus, the Pgp3-based immunofluorescence assay has allowed us to identify authentic plasmid-free organisms that are useful for further investigating chlamydial pathogenic mechanisms.

• Smart Structures and Systems
• /
• v.30 no.2
• /
• pp.135-143
• /
• 2022

The object of this paper is to investigate the free vibration behavior under the effect of carbon nanotube distribution in functionally graded carbon nanotube-reinforced composite (FG-CNTRC) by using higher-order shear deformation theories. In this work, we present a novel distribution method for carbon nanotubes in the polymer matrix by using a new exponential power law distribution of carbon nanotube volume fraction. It is assumed that the SWCNTs are aligned along the beam axial direction and the distribution of the SWCNTs may vary through the thickness of the beam with different patterns of reinforcement. The rule of mixtures is used in order to obtain material properties of the CNTRC beams. Hamilton's principle is used in deriving the equations of motion. The validity of the free Vibration results is examined by comparing them with those of the known data in the literature. The results that obtained indicate that the carbon nanotube volume fraction distribution play a very important role on the free vibrations characteristics of the CNTRC beam.