Journal of Korean Society of Coastal and Ocean Engineers
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v.29
no.6
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pp.369-381
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2017
It has been presumed that highly nonlinear skewed waves frequently observed in a surf zone could significantly influence the transmission behaviour via a porous wave breaker due to its larger inertia force than its nonlinear counterparts of zero skewness [Cnoidal waves]. In this study, in order to confirm this perception, a numerical simulation has been implemented for 6 waves the skewness of that range from 1.02 to 1.032. A numerical simulation are based on the Tool Box called as the ihFoam that has its roots on the OpenFoam. Skewed waves are guided by the shoal of 1:30 slope, and the flow in the porous media are analyzed by adding the additional damping term into the RANS (Reynolds Averaged Navier-Stokes equation). Numerical results show that the highly nonlinear skewed waves are of higher transmitted ratio than its counterparts due to its stronger inertia force. In this study, in order to see whether or not the damping at the porous structure has an effect on the wave celerity, we also derived the dispersive relationships of Nonlinear Shallow Water Eq. [NSW] with damping at the porous structure being accounted. The newly derived dispersive relationships shows that the phase lag between the damping friction and the free surface elevation due to waves significantly influence the wave celerity.
Pinealocytes in the lower vertebrate are known to have photoreceptive function. These photoreceptor cells have been characterized morphologically in various species of lower vertebrates. No such ultrastructural studies, however, were reported in fresh water turtle. The purpose of this study is to characterize the pinealocytes and the phylogenetic evoluton of these cells is discussed in terms of functional analogy. I. Light microscopy: The pineal body was divided into incomplete lobules by connective tissue septa containing blood vessels, and parenchymal cells were arranged as irregular cords or follicular pattern. In the lobules, glandular lumina were present and contained often densely stained materials. II. Electron microscopy: The pineal parenchyma had three categories of cells: photoreceptor cells, supportive cells and nerve cells. The photoreceptor cells had darker cytoplasm compared to the supportive cells, and the enlarged apical cytoplasm(inner segment) containing abundant mitochondria and dense cored vescles protruded into the glandular lumen in which lamellar membrane stacks(outer segment), dense membranous materials, and cilia were present. Some of these lamellated membrane stacks appeared to be dege-nerating while others were apparently newly formed. Constricted neck portion of the photoreceptor cells contained longitudinally arranged abundant microtubules. centrioles and cross-striated rootlets. Cell body had well developed Golgi apparatus, abundant mitochondria, dense granules($0.5{\sim}1{\mu}m$), dense cored vesicles($70{\sim}100nm$), and rough endoplasmic reticulum occasionally with dense material within its cisterna. Basal portion of the photoreceptor cells had basal processes often with synaptic ribbons, which terminate in the complicated zone of cellular and neuronal processes. Synatpic ribbons often made contact with the nerve processes and the cell processes of neighboring cells. In some instances, these ribbons were noted free within the basal process and were also present at the basal cell mem-brane facing the basal lamina. Obvious nerve endings with clear and dense cored vesicles were observed among the parenchymal cells. Photoreceptor cells of the snapping turtle pineal body were generally similar in fine structure to those of other lower verterbrates reported previously, and suggested to have both photoreceptive and secretory functions which were modulated by pinealofugal and pinealopedal nerves. The supportive cells were characterized by having large dense granules($0.3{\sim}1{\mu}m$), abundant ribosomes, well developed Golgi apparatus and rough endoplasmic reticulum. These cells were furnished with microvilli on the luminal cell surfaces, and often had centrioles, striated rootlets, abundant filaments especially around the nucleus, and scattered microtubules. Some supportive cells had cell body close to the lumen and extended a long process reaching to basal lamina, which appeared to be a glial cell. Nerve cells within the parenchyma were difficult to identify, but some large cells located basally were suspected to be nerve cells, since they had synaptic ribbon contact with photoreceptor cells.
Background: A previous study has shown that Euonymus alatus (EA) has an antidotic activities against inflammation, suggesting possibility that EA can exert this beneficial effects to liver injury by an initial protection against drug-induced hepatocyte demage. The present study was undertaken to evaluate the protective effect of EA-extract on experimentally induced hepatitis in ICR mice and to investigate some mechanisms responsible for its action. Methods: Water EA extract was used in this experiments. The mice received i.p. a dose of 700 mg/kg galactosamine (GalN) together with $5{\mu}g/kg$ of endotoxin (LPS), or received i.v. 12 mg/kg of concanavalin A (Con A). EA (4 mg/mouse) was administrated on day -2, -1 and 0 before induction of liver injury. Liver injury was assessed by measurement of serum alanin amino-transferase (SGPT) levels on 9 hr after GaIN.LPS, or 8 hr after con A administration. Results: Treatment with either GaIN or LPS alone did not cause hepatitis. However, simultaneous administration of GalN and LPS to mice resulted in LPS-dose dependent fulminant hepatitis. GaLN/LPS-induced liver injury was reduced when mice were given EA for 3 days before induction. This preventive effect of Ea was more prominent when EA was given by intraperitoneal route rather then by oral route. Pretreatment of EA or dexamethasone inhibited significantly $TNF{\alpha}$ production in GalL/LPS-injured mice. However, EA-treatment did not influence $TNF{\alpha}$-induced hepatitis in GalN-sensitized mice, suggesting that $TNF{\alpha}$ is likely to act as one of final mediators of endotoxin action and the protective effect of EA might be manifested chiefly by inhibition of endotoxin-induced $TNF{\alpha}$ production, not by blocking the $TNF{\alpha}$-action. Injection of Con A into mice evoked remarkable liver injury in a dose dependent fashion. This liver damage was reduced by EA-pretreatment. Dexamethasone significantly reduced both GalL/LPS-induced and Con A-induced liver damages, showing synergism with EA. However, indomethacin reduced only GalN/ LPS-induced hepatitis, not for Con A-induced hepatitis. Conclusion: These results led to the conclusion that EA may be able to contribute at least in part to prevent the drug-induced hepatotoxicity, and that its anti-hepatitis effects might be manifested directly by modulation of endogenous mediators, such as leukotriese D4, $TNF{\alpha}$ and free radical, and indirectly by regulation of immune mediated responses. Also these results suggested that EA could be developed as a potential antidotic agent.
This study was carried out to plan the prevention of the generation and discharge of Acid Rock Drainage (ARD). The Acid Base Accounting(ABA) test was performed for geological materials such as pit wall, waste rock and stream sediments near the Imgi abandoned pyrophyllite mine in Busan, Korea. In addition, hydraulic characteristics were tested with the disk tension infiltrometer around the waste rock dump. Maximum Potential Acidity(MPA) of geological materials near the Imgi mine was 246.942kg $H_2SO_4/t$, and maximum Acid Neutralising Capacity(ANC) was 8.7kg $H_2SO_4/t$. These results indicate the pit wall and waste rock, except most of stream sediments are acid generating geological materials. These have salt and free hydrogen ion which resulted from oxidation of sulfides. Hence they could be convert rain water to acid rock drainage. Although the waste rock dump of the Imgi mine have very low infiltration rate, slopes of the waste rock dump have many "V" type erosion gullies and multi-layers. These gullies and multi-layers have coarse clastic particle layers which have very large hydraulic conductivity. Through this coarse clastic particle layer a large part of rain flow into ground. And also this layer could function as aeration path which induced oxidation of sulfide minerals and generation of ARD continuously.
Journal of the korean academy of Pediatric Dentistry
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v.24
no.1
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pp.204-219
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1997
The use of fluoride is one of the most effective methods for caries prevention. Fluoridation of public water supply has been recognized, for many years, as an effective way to reduce dental caries. The fluoride supplement has been recommended when the natural fluoride was unavailable or below the optimal range. However the mechanism of caries prevention by fluoride has not yet been clarified and it is well known that an overdose of fluoride results inacute and chronic toxicity, especially dental fluorosis. Fluoride mouthrinsing solution is widely used in dentistry due to its effectiveness in carrying anticariogenic action. Understanding the effects of fluoride mouthrinsing solution on human gingival fibroblasts will provide the safety rationale for its use during the caries preventive therapy. The purpose of this study was to evaluate the cytotoxic effect of fluoride mouthrinsing solution on the human gingival fibroblast in vitro. The human gingival fibroblasts were cultured from healthy gingiva on the extracted deciduous teeth of children. Cells were inoculated into a 24-well plate with $1{\times}10^4cells/well$ of medium at $37^{\circ}C$, 100% humidity, 5% $CO_2$ incubator for 24 hours. And the cells were counted by using the hemocytometer at each designed study. Human gingival fibroblasts were cultured in growth medium after one minute application range of 0.02%-0.2% NaF solution and 0.1% $SnF_2$ solution. The cells used in this study were between fifth to eighth passage number. The cell morphology was examined by inverted microscope and cell proliferation was measured by incorporating $[^3H]$-thymidine into DNA. DNA synthesis by human gingival fibroblasts was assessed by $[^3H]$-thymidine uptake assays while the cell activity was measured by MTT assay. Each concentrated fluoride mouthrinsing solution was estimated for its biocompatability with fibroblasts by the tissue culture technique. The results of this study were as follows : 1. It was observed that at 0.05%, 0.2% NaF mouthrinsing solution the cytoplasmic processes became globular. When 0.1% $SnF_2$ mouthrinsing solution was applied, the cytoplasmic process and cell morphology were disappeared. 2. DNA synthetic activity was reduced regardless of the concentration of the fluoride mouthrinsing solution. However, the result is statistically insignificant except 0.1% $SnF_2$ mouthrinsing solution(p<0.05). 3. Our results indicate that 0.02%, 0.05% concentrations of NaF mouthrinsing solution caused minimal cytotoxicity. But 0.2% NaF and 0.1% $SnF_2$ concentration were a significant difference between the cell activity in the experimental group and control group (p<0.05). 4. After appling 0.05% & 0.02% NaF fluoride mouthrinsing solution, cell activity was restored to the control groups level according to incubating time. The results suggest that direct exposure to fluoride solution inhibits gingival fibroblast activity. Therefore, for the most effective use of fluoride use, lowering the concentration of fluoride mouthrinsing is advisable because it maintains biocompatability and free ion in the oral fluid.
Chand, N.;Muhammad, Din;Durrani, F.R.;Qureshi, M. Subhan;Ullah, Sahibzada S.
Asian-Australasian Journal of Animal Sciences
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v.24
no.7
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pp.1011-1018
/
2011
Aflatoxin-contaminated feed cause mortality, suppression of the immune system, reduced growth rates and losses in feed efficiency. This research study was planned to investigate the immunomodulatory and growth promoting effect of milk thistle as feed additive against aflatoxin $B_1$ in broiler chicks at NWFP Agricultural University Peshawar, Pakistan. Two hundred and forty (240) day old broilers chicks were randomly assigned into four major groups AfF, aflatoxin free feed; Aflatoxin $B_1$ was present in the feed at the levels of 80-520 ${\mu}g/kg$ of the feed in the remaining three groups. Aflatoxin contaminated feed was provided for 5 weeks. Group AfB was supplemented with toxin binder "Mycoad" at 3 g/kg of feed and group AfT was supplemented with milk thistle at10 g/kg of feed. Each group was further sub divided into two sub-groups, vaccinated against ND (Newcastle disease), IB (Infectious bronchitis) and IBD (Infectious bursal diseases) according to recommended schedule of vaccination or non vaccinated. Each sub group carried three replicates with 10 chicks per replicate. Chicks were reared in pens in an open sided house. Supplementary heat was provided to all the chicks during brooding period. Mean body weight gain and dressing percentage were significantly (p<0.05) higher in group AfF, followed by AfT, AfB and Af. Weight gain and dressing percentage was the same in group AfB and AfT, while it was significantly lower in group Af. Feed intake, breast, thigh and leg weight were found significantly (p<0.05) higher in group AfF, followed by AfB, AfT and Af. Significantly lower (better) FCR value was recorded in group AfT. Water intake was significantly (p<0.05) higher in group AfT and AfF as compared to other groups. Mortality was significantly (p<0.05) higher in group Af. Mean bursa and thymus weights were found significantly (p<0.05) higher in group AfF, AfB and AfT followed by Af, while higher spleen weight was recorded in group AfT. Mean antibody titer against ND, IB and IBD was significantly (p<0.05) higher in group AfT, as compared to other groups. It is concluded that milk thistle at 10 g/kg of feed could effectively be utilized as immunostimulant and growth promotant in the presence of immunosuppressant aflatoxin $B_1$ in the feed.
Kim, Eunkyung;Chang, Yoon Hyuk;Ko, Jae Youn;Jeong, Yoonhwa
Journal of the Korean Society of Food Science and Nutrition
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v.42
no.11
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pp.1821-1828
/
2013
This study was conducted to investigate the physicochemical and microbial properties of Makgeolli supplemented with kiwifruit (Actinidia deliciosa). Four hundred grams of kiwifruit were added to 3.1 L of distilled water, followed by 2.0 kg of rice, 40.0 g of Nuruk, and 14.0 g of yeast. The mixed rice solution was then fermented at $28^{\circ}C$ for 6 days to prepare the kiwifruit Makgeolli. The pH values of the kiwifruit Makgeolli decreased from 5.31 to 4.37, but the total acidity values increased from 0.05 to 0.34% during fermentation. The total viable cells ($3.18{\times}10^7$ and $2.88{\times}10^7$, respectively), lactic acid bacteria ($1.51{\times}10^6$ and $1.50{\times}10^6$, respectively), and yeast counts ($1.96{\times}10^7$ and $1.90{\times}10^7$, respectively) of the kiwifruit Makgeolli and control were similar throughout the fermentation process. Glucose was the major free sugar in the control and kiwifruit Makgeolli and significantly decreased during fermentation. Succinic acid was the highest organic acid in both the control (24.6 mg/mL) and kiwifruit Makgeolli (26.3 mg/mL). In a volatile compound analysis, 3-methyl-1-butanol, 2-methyl-1-propanol and ethyl acetate were the major volatile compounds in the kiwifruit Makgeolli.
Park, Ji-Young;Baek, Dong-Won;Nili, Mohammad;Kim, Jin-Kyu
Korean Journal of Environmental Biology
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v.29
no.4
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pp.258-264
/
2011
Glutathione (GSH) has important roles in cellular defense against oxidative stress, 1) direct scavenging of reactive oxygen species (ROS), and 2) coenzyme of ROS scavenging enzyme like glutathione peroxidases (GPx). GSH peroxidase reduces free hydrogen peroxide to water using 2GSH. $N$-acetyl-L-cysteine (NAC), one of the antioxidants, is used as a precursor for intracellular GSH. In this study, relation of GSH, NAC, and GSH peroxidase was investigated through transcriptional expression of $GPX1$ and $GPX2$, which are GSH peroxidase encoding genes, in yeast cells treated with 0 mM to 20 mM of NAC or in combination with 100 Gy gamma-rays. The transcriptional expression of $GPX1$ and $GPX2$ was induced by NAC and 100 Gy gamma-rays. The gene expression of both GSH peroxidases was decreased with increasing concentrations of NAC in irradiated yeast cells. These results suggest that elevation of intracellular GSH by NAC and oxidative stress and ROS generated from gamma-rays induces expression of GSH peroxidase genes, and that NAC can protect the yeast cells against ROS generated from gamma-rays through direct scavenging of ROS and transcriptional activation of GSH peroxidase.
This study performs to evaluate the role of filter material at alluvial well for intake of riverbank filtration and the applicability and improvement effect of dual filter well. To achieve this objective, dual filter intake well and single filter intake well were installed with different filter conditions at riverbank free surface aquifer in soil layer then we evaluated filter material condition, permeability, optimum yield and well efficiency according to yield in drawdown test. As a results, we assumed forming dual filter layer minimizes sudden speed changes at boundary between aquifer and filter layer by cushioning of groundwater flow. This suppresses warm current then intake groundwater efficiently, therefore it seems decreasing peripheral groundwater level changes in spite of higher intake water amount than single filter intake well. Furthermore, we confirmed by test, installing dual filter improves permeability, optimum yield and well efficiency. The result will be used by combining with former study to set up standard of design/construction of dual filter intake well at alluvial aquifer layer. Furthermore, we expect this result will be used to prove application effect of dual filter intake well compared to single filter one and radial collector well which are mainly applied on riverbank filtration.
Kim, Dea-Wook;Heo, Hwa-Young;Suh, Sae-Jung;Lee, Yeong-Ho;Kim, Si-Ju
KOREAN JOURNAL OF CROP SCIENCE
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v.51
no.spc1
/
pp.133-138
/
2006
To differentiate barley responses to drought and salt stress, barley seedlings at the second leaf stage were treated with 218 mM NaCl and 29.5% PEG6000 iso-osmotic to 218 mM NaCl for 6 days. Shoot fresh weight and leaf relative water content of barley seedlings were more reduced by drought compared to salt stress. Hydrogen peroxide content increased under both stress conditions, but its accumulation was more severe at 6 days after salt stress. The activity of ascorbate peroxidase, glutathione reductase (GR) and catalase (CAT) was enhanced until 4 days after salt stress. On the other hand, the activity of GR and CAT increased gradually until 6 days after drought. Among the amino acids measured in this study, the accumulation of glycine, arginine and GABA (${\gamma}-aminobutyric$ acid) was lower under salt stress than drought. However, considerably larger amount of proline was accumulated by salt stress. It is concluded that the antioxidant enzymes activity and amino acid content of barley seed-lings were differently regulated in response to the isoosmotic condition of salt and drought stress.
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