Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Gene Expression of Glutathione Peroxidase in $Saccharomyces$ $cerevisiae$ Treated with $N$-acetyl-L-cysteine and Gamma-rays

$Saccharomyces$ $cerevisiae$에서 $N$-acetyl-L-cysteine 처리와 감마선 조사에 따른 Glutathione Peroxidase 유전자 발현

  • Park, Ji-Young (Korea Atomic Energy Research Institute, Advanced Radiation Technology Institute) ;
  • Baek, Dong-Won (Division of Applied Life Sciences (World Class University Program), Gyeongsang National University) ;
  • Nili, Mohammad (Dawnesh Radiation Research Institute) ;
  • Kim, Jin-Kyu (Korea Atomic Energy Research Institute, Advanced Radiation Technology Institute)
  • 박지영 (한국원자력연구원 방사선과학연구소) ;
  • 백동원 (경상대학교 대학원 응용생명과학부) ;
  • 모하마드닐리 (스페인 도네시방사선연구소) ;
  • 김진규 (한국원자력연구원 방사선과학연구소)
  • Received : 2011.08.16
  • Accepted : 2011.10.18
  • Published : 2011.11.30
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Abstract

Glutathione (GSH) has important roles in cellular defense against oxidative stress, 1) direct scavenging of reactive oxygen species (ROS), and 2) coenzyme of ROS scavenging enzyme like glutathione peroxidases (GPx). GSH peroxidase reduces free hydrogen peroxide to water using 2GSH. $N$-acetyl-L-cysteine (NAC), one of the antioxidants, is used as a precursor for intracellular GSH. In this study, relation of GSH, NAC, and GSH peroxidase was investigated through transcriptional expression of $GPX1$ and $GPX2$, which are GSH peroxidase encoding genes, in yeast cells treated with 0 mM to 20 mM of NAC or in combination with 100 Gy gamma-rays. The transcriptional expression of $GPX1$ and $GPX2$ was induced by NAC and 100 Gy gamma-rays. The gene expression of both GSH peroxidases was decreased with increasing concentrations of NAC in irradiated yeast cells. These results suggest that elevation of intracellular GSH by NAC and oxidative stress and ROS generated from gamma-rays induces expression of GSH peroxidase genes, and that NAC can protect the yeast cells against ROS generated from gamma-rays through direct scavenging of ROS and transcriptional activation of GSH peroxidase.

Glutathione (GSH)은 직접적으로 활성산소종을 제거하거나 GSH peroxidase와 같은 활성산소종 제거 효소의 조효소로써, 산화적 스트레스로부터 세포를 방어하는 데 중요한 역할을 한다. GSH peroxidase는 두 분자의 GSH을 이용해 세포 내 과산화수소를 물로 전환한다. $N$-acetyl-L cysteine (NAC)는 항산화제 중 하나로 세포 내 GSH의 전구물질로 이용된다. 본 연구는, 0mM에서 20mM의 NAC 단독 처리 또는 100 Gy 감마선과 복합 처리한 효모세포에서 GSH peroxidase를 코드화(encoding)하는 유전자인 $GPX1$과 $GPX2$의 전사적 발현을 통해 GSH, NAC와 GSH peroxidase의 연관성을 알아보았다. $GPX1$과 $GPX2$의 전사적 발현은 NAC와 100 Gy 감마선에 의해 유도되었다. 조사된 효모세포에서 NAC의 증가 농도에 따라 GSH peroxidase 두 유전자의 발현은 감소되었다. 이러한 결과로, NAC에 의해 증가된 세포 내 GSH는 GSH peroxidase 유전자의 전사적 발현을 유도하며, NAC는 감마선으로부터 생성된 활성산소종 직접적 제거와 GSH peroxidase 유전자의 전사적 발현을 유도함으로써 세포를 보호할 수 있다는 것이 밝혀졌다.

Keywords

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