• Title/Summary/Keyword: Free Fatty Acids

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Antioxidant Activity of Green Tea Extract in Soybean and Rice Bran Oils

  • Kim, Chang-Soon;Park, Jae-Hee
    • Preventive Nutrition and Food Science
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    • v.7 no.2
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    • pp.151-156
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    • 2002
  • Antioxidant activity or green tea extracts (GTE) was evaluated in soybean oil (SBO), rice bran oil (RBO) and winterized rice bran oil (WRBO) stored at 63$^{\circ}C$ for 36 days. Lipid oxidation of the oils was determined using the active oxygen method (AOM), peroxide value (POV), change in unsaturated free fatty acid concentrations and by sensory evaluation. SBO had a higher concentration of the polyunsaturated fatty acids, linoleic and linolenic acid than RBO and WRBO. WRBO and RBO were more stable against lipid oxidation than SBO. Addition of GTE (200 ppm) to the stored oils, increased the induction period (IP) in AOM, reduced the increase in POV, and lessened the change in unsaturated fatty acids. Furthermore, GTE prevented the development of rancid flavors resulting from storage, all of which demonstrate the protective antioxidative activity of GTE. However, oil color became darker in the GTE treated oils. The antioxidant protection of GTE was most effective in RBO.

Lipid Studies of Carum Roxburghianum Seeds

  • Waheed, Amran;Mahmud, Shahid;Saleem, Muhammad;Yamin, Muhammad;Khan, Muhammad Naeem
    • Natural Product Sciences
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    • v.9 no.3
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    • pp.200-203
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    • 2003
  • Total lipids extracted from the powdered seeds of Carum roxburghianum were fractionated into hydrocarbons (0.30%), wax esters (0.30%), sterol esters (1.35%), triacylglycerols (72.41%), free fatty acids (6.06%), 1,3-diacylglycerols (4.60%), 1,2- diacylglycerols (0.64%), glycolipids (5.10%), sterols (1.20%), 2-monoacylgylcerols (3.18%), 1-monoacylglycerols (1.46%), phosphatidylethanolamines (1.08%) phosphatidylcholines (0.40%), lysophosphatidylethanolamines (1.48%) and phosphatidylinositols (0.44%) with the help of TLC. The fatty acid composition of all the lipid fractions was determined after converting them into their methyl esters with $BF_3-methanol$ reagent and then analyzing them by GC. Oleic acid was found as a major component in all the lipid classes, whereas palmitic, linoleic and linolenic acids were present in lesser quantities. Arachidic acid was identified as a minor component in only seven out of twelve lipid classes.

Seasonal Changes in Chemical Components of the Leaves of Dendropanax morbifera Lev. (채취시기(採取時期)에 따른 황칠(黃漆)나무 잎의 화학성분(化學性分) 변화(變化))

  • Kim, Hyung-Ryang;Chung, Hee-Jong
    • Journal of Korean Society of Forest Science
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    • v.88 no.4
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    • pp.562-567
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    • 1999
  • Dendropanax morbifera Lev. leaves were collected during different seasons to analyze the chemical components. In proximate composition the leaves collected in spring had the highest moisture content and in winter had the lowest. Lipid, ash and fiber contents gradually increased from spring to winter and in winter had the highest. Protein content decreased until summer and then it increased. All of free sugars detected have gradually increased during the growing season. Unsaturated fatty acids content was higher than that of the saturated fatty acids and the major saturated and unsaturated fatty acids were arachidic acid and cis-13,16-docosadienoic acid. respectively. Fifteen free amino acids detected with arginine content being the highest regardless of season and amino acid content gradually decreased during the seasons. The highest content of mineral elements was potassium in leaves collected in spring, with calcium was the highest in leaves picked in other seasons. Vitamin C content was gradually decreased over the growing season and soluble tannin content increased notably.

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Physicochemical Properties of the Turmeric (Curcuma longa L.) in Jindo Korea (진도산 울금(Curcuma longa L.)의 이화학적 특성)

  • Oh, Da-Young;Kim, Han-Soo
    • Journal of Environmental Science International
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    • v.28 no.4
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    • pp.403-412
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    • 2019
  • Studies on the physicochemical properties of physiological activity substance in turmeric (Curcuma longa L.) were analyzed for the use as an functional food materialization. The proximate compositions in the vacuum freeze dried turmeric were carbohydrate 72.90%, moisture 5.74%, crude protein 10.02%, crude fat 4.67%, and crude ash 6.69%, respectively. The mineral contents of turmeric were calcium (Ca) $2,294.77mg\;kg^{-1}$, potassium (K) $28,780.54mg\;kg^{-1}$, magnesium (Mg) $2,826.90mg\;kg^{-1}$, sodium (Na) $1,826.58mg\;kg^{-1}$, iron (Fe) $190.94mg\;kg^{-1}$, and manganese (Mn) $620.16mg\;kg^{-1}$. The vitamin contents of turmeric were pantothenic acid 1.040 mg/100 g, riboflavin 0.166 mg/100 g, thiamin 0.148 mg/100 g, pyridoxine 0.010 mg/100 g, and calciferol 0.008 mg/100 g, respectively. Total amino acid contents in protein of turmeric were 7.66 g%, and major amino acids were aspartic acid 1.45 g%, glutamic acid 1.07 g%, leucine 0.71 g%, phenylalanine 0.47 g%, and arginine 0.46 g%, respectively. The amount of free amino acids of turmeric were 225.81 mg%, and major free amino acids were asparagine, aspartic acid, glutamic acid, serine, and alanine. Especially, in the case of asparagine, it was highest. The compositions of fatty acid were saturated fatty acid 45.09%, monoenes 8.62%, and polyenes 46.30%.

Comparison of Physicochemical and Sensory Properties of Freeze-concentrated Milk with Evaporated Milk during Storage

  • Hwang, J.H.;Lee, S.J.;Park, H.S.;Min, S.G.;Kwak, H.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.2
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    • pp.273-282
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    • 2007
  • This study was carried out to compare the changes of nutrients, sensory and chemical properties of freeze-concentrated and evaporated milks during storage. For pasteurization, the freeze-concentrated milk containing 27% of total solid was treated with 150 rpm ozone for 5 min, and 99% of microflora was eliminated. Also, the activities of protease and lipase decreased 93.31% and 96.15%, respectively, and phosphatase showed negative activity. Total bacteria count was maintained below$2.0{\times}10^4$CFU/ml. During storage, TBA absorbance was lower in freeze-concentrated milk than that in the evaporated milk. The production of short-chain free fatty acids and free amino acids increased proportionally to the storage period in both samples. While the short-chain free fatty acid production was lower in the freeze-concentrated milk compared with that in the evaporated milk, the production of individual free amino acid was similar in both samples. In sensory evaluation, cooked flavor and color were much lower in the freeze-concentrated milk than that in the evaporated milk. Overall acceptability score was higher in the freeze-concentrated than the evaporated milk. Based on above results, ozone treatment for the freeze-concentrated milk pasteurization was positive at the elimination of microflora and enzyme inactivation. During storage, the freeze-concentrated sample minimized the change of color and TBA absorbance, the production of short-chain free fatty acid and vitamins than the evaporated milk. Therefore, the freeze-concentrated milk process in the present study resulted in the positive effect in minimizing nutrient loss and keeping quality of milk during storage.

Seasonal Variations of Nutrients in Warty Sea Squirt(Styela clava) (계절에 따른 미더덕의 영양성분 조성에 관한 연구)

  • 이강호;박천수;홍병일;정병천;조호성;제외권
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.2
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    • pp.268-273
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    • 1995
  • To study the nutritional value of warty sea squirt, Styela clava, which is one kind of urochoda and it has been used as special seafood stuffs by Korean, the seasonal variations of nutrient was investigated. The moisture content was ranged from 83.6% to 86.8% in experimental period. Maximun glycogen content showed up in June (3.7g/100g sample). The protein(N$\times$6.25) and lipid content varied with glycogen whereas ash content was not changed remarkably, showing 2.8$\pm$0.3%. Predominant minerals in edible portion and integuments were sodium, potassium, calcium and magnesium in order. Predominant constitutiional amino acids were asparagine, glutamic acid, taurine, aspartic acid, lysine and glycine and amino acids occupied 50% of the total amino acid. Porportion of nonpolar lipid to total lipid increased from April to early June while polar lipid level decreased. The neutral lipid was composed of triglyceride(59.32%) and free sterol(23.52%), and followed by diglycerides, monoglycerides, esterified sterols and hydrocarbon, free fatty acid. The phospholipid was mainly composed with phosphatidyl choline(49.7%), and phosphatidyl ethanolamine(33.0%). The major fatty acids of the total lipid in warty sea squirt were C20 : 5(17%), C22 : 6(13.76%), C16 : o(13.91%) and C16 : 1(12.52%).

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Effect of Shading Period on Contants of Inorganic Components, Free Amino Acids and Fatty Acids in Thea Sinensis L. (차광기간(遮光期間)이 다엽(茶葉)의 무기성분(無機成分), 아미노산(酸), 지방산(脂肪酸) 함양(含量)에 미치는 영향(影響))

  • Park, Jang-Hyun;Choi, Hyoung-Koog
    • Korean Journal of Medicinal Crop Science
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    • v.4 no.3
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    • pp.247-254
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    • 1996
  • The effect of shading on mineral elements, free amino acids, and fatty acids content of tea shoot was examined under different shading periods. The results are summarized as follows. The con­tent of $NH_4^+$, $K^+$. $Mg^{++}$, $PO_4^{--}, was increased, but that of $Na^+$, $Ca^{++}$, $F^-$, $Cl^-$, $No_3^-$, $So_4^{--}$ was de­creased with the passage of shade treatment periods. The content of Total- Nitrogen of tea shoot was the highest in the 55% 10days+95% 5days shading as 6.07%. The content to Total Free Amino Acid was hagher ranged from 20mg/100g to 80mg/100g in shading treatment compered with the un shading treat­ment. The content of Theanine was the highest in 55% 10days+95% 5days shading as 1834mg/100g,while that of unshading was the lowest as 1247mg/100g. The content of Theanine was decreased with the passage of shading periods. The content of Fatty Acid was the highest in the 55% 15days+95% 15days shading as 3164mg/100g, while the unshading treatment had the lowest in the 55% 5days+95% 5days as 2435mg/100g.

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Changes in Fat in Gouda Cheese by the Psychrotrophic Bacterium Acinetobacter Genomospecies 10 (내냉성미생물인 Acinetobacter genomospecies 10에 의한 gouda cheese의 지방의 변화)

  • Shin, Yong Kook;Oh, Nam Su;Lee, Hyun Ah;Nam, Myoung Soo
    • Journal of Life Science
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    • v.25 no.2
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    • pp.174-179
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    • 2015
  • The presence of psychrotrophic bacteria downgrades the quality of dairy products. This study evaluated the effect of lipolytic psychrotrophic bacteria on the chemical properties of Gouda cheese made from raw milk experimentally inoculated with a psychrotrophic bacterium (Acinetobacter genomospecies 10). Raw milk experimentally inoculated with Acinetobacter genomospecies 10 and refrigerated at $4^{\circ}C$ for 3 or 6 days produced a 6-week ripened Gouda cheese with a significant decrease in total solids (p<0.05) or an increased fat content (p<0.05), respectively. Raw milk inoculated with Acinetobacter genomospecies 10 and refrigerated for 3 days had higher (p<0.05) SCFFA (1.35 times), MCFFA (1.42 times), and LCFFA (1.44 times) than the control 6-week ripened Gouda cheese. The cheese manufactured from the inoculated and refrigerated raw milk had higher (p<0.05) total free fatty acids (1.68 times) compared with the control. Raw milk inoculated with Acinetobacter genomospecies 10 and refrigerated for 6 days had increased SCFFA (1.45 times), MCFFA (1.28 times), and LCFFA (1.38 times) compared with the control 6-weeks ripened Gouda cheese. The 6-week ripened Gouda cheese manufactured from this inoculated milk had higher (p<0.05) total free fatty acids (1.34 times) compared with the control. The results indicated that the production of excessive free fatty acids in dairy products by psychrotrophic bacteria can be critical in predisposing dairy products to off-flavors and in turn degrading their quality.

Changes in Chemical Characteristics of Roasted Red Pepper Powder with Lard during Storage (돈지가 첨가된 조제고춧가루의 저장 중 화학적 특성 변화)

  • Yang, Jong-Beom;Ko, Myung-Soo;Jung, In-Chul;Moon, Yoon-Hee
    • Food Science of Animal Resources
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    • v.28 no.3
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    • pp.256-262
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    • 2008
  • To develop a new quality control method for the evaluation of Korean instant noodle soups, the chemical characteristics of roasted red pepper powder (RRP), mixed with lard were investigated while in storage at $65^{\circ}C$ for 6 weeks. The moisture contents of the RRP increased but the crude protein and crude lipid contents decreased up to 4 weeks of storage. The pH value decreased and the acid value increased steadily during storage. Both the American Spice Trade Association (ASTA) value that indicates redness of red pepper, and the CIE L, a, and b values decreased remarkably during storage. The fatty acids of the RRP oil were primarily oleic acid (33.4%), linoleic acid (30.8%), and palmitic acid (21.2%). The composition of fatty acids did not significantly change after 6 weeks of storage (p>0.05). Regarding the free fatty acid (FFA) composition of the RRP oil, palmitic acid (36.5%) was the principal component. The total amount of FFA and the amount of each individual FFA increased remarkably during storage. In addition, the ratio of free unsaturated fatty acids to free saturated fatty acids increased during storage.

Studies on the Lipid Composition in Three Species of Shellfish (3종(種) 패류(貝類)의 지질조성(脂質組成)에 관한 연구(硏究))

  • Son, Young Ock;Ha, Bong Seuk
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.12 no.4
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    • pp.407-419
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    • 1983
  • In this study, the lipid components of three species of shellfish included oyster(Crassostrea gigas), top shell(Turbo cornutus) representing salt water shellfish and corb shell(Corbicula fluminea producta) representing flesh water shellfish were analysed and nutriontional significances were discussed. Analysed the total lipid composition, and the fatty acid and sterol composition of total lipid were determined. The lipid was fractionated into three lipid classes neutral, glyco and phospholipid by column chromatography. The fatty acid composition of each lipid class and sterols were determined by gas liquid chromatography. The lipid components of total lipid and neutral lipid were estimated by thin layer chromatography and TLC scanner. The results were as follows: Total lipid contents of shellfish were 1.8% in oyster, 0.4% in top shell and 4.0% in corb shell. The contents of total fatty acid in total lipid were 80.7, 71.2 and 73.2%; and the contents of unsaponifiable matters were 15.4, 18.1 and 23.1% respectively. Total lipids were mainly composed of triglycerides, polar lipid-pigments and sterols as major component, and hydrocarbon-esterified sterols were determined in each sample. The major fatty acids in total lipid were palmitic(37.0%), eicosapentaenoic(13.5%) and linoleic acid(11.2%) in oyster, Octadecatetraenoic(15.8%), palmitic(11.2%), oleic(8.6%) and linoleic acid(8.1%) in top shell, but palmitic(34.0%), linoleic(12.3%) and paimitoleic acid(9.8%) in corb shell. Particularly, the contents of eicosapentaenoic acid of oyster and top shell were higher than those of corb shell. Sterol composition from three species of shellfish were mainly consisted of cholesterol (42.7~64.0%), brassicasterol(15.6~24.7%) and 24-methylenecholesterol (4.7~21.9%). But sitosterol (5.3%) was detected only in oyster and 22-dehydrocholesterol(12.9%) was only in top shell. The contents of fractionated neutral lipid was commonly higher than that of polar lipid in each sample. Glycolipid and phospholipid in polar lipid showed similar in quantity. The neutral lipids were composed of triglycerides(33.0~36.7%), free sterols(25.7~31.2%), esterified sterol(12.4~23.7%) and free fatty acids(5.1~11.7%). The contents of triglycerides and free sterols were higher than those of free fatty acids and esterified sterols. The major fatty acids in neutral lipid were palmitic(28.4~26.4%) eicosapentaenoic(18.6~21.9%) and linoleic acid(9.0~5.4%) in oyster and corb shell but octadecatetraenoic(14.5%), eicosapentaenoic (13.5%) and palmitic acid(12.3%) in top shell. The major fatty acids in glycolipid were eicosenoic(10.2%), palmitic(12.1%) and linolenic acid (10.2%) in oyster, Eicosenoic(26.0%), octadecatetraenoic(14.6.%) and eicosadienoic acid(12.9%) in top shell. But eicosadienoic(21.4%) stearic(14.6%), octadecatetraenoic(8.5%) and eicosenoic acid(8.5%) in corb shell. The major fatty acids in phospholipid were myristic(16.0%), stearic(10.6%), eicosenoic(10.5%) and palmitic acid(10.3%) in oyster, Oleic(22.2%), stearic(20.7%) and linolenic acid (11.8%) in top shell but eicosapentaenoic(25.1%), myristic(8.7%) and arachidonic acid(8.3%) in corb shell.

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