• BMB Reports
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• 제40권3호
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• pp.333-340
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• 2007

Gene encoding cyclodextrin glycosyltransferase (CGTase), from thermotolerant Paenibacillus sp. T16 isolated from hot spring area in northern Thailand, was cloned and expressed in E. coli (JM109). The nucleotide sequences of both wild type and transformed CGTases consisted of 2139 bp open reading frame, 713 deduced amino acids residues with difference of 4 amino acid residues. The recombinant cells required 24 h culture time and a neutral pH for culture medium to produce compatible amount of CGTase compared to 72 h culture time and pH 10 for wild type. The recombinant and wild-type CGTases were purified by starch adsorption and phenyl sepharose column chromatography and characterized in parallel. Both enzymes showed molecular weight of 77 kDa and similar optimum pHs and temperatures with recombinant enzyme showing broader range. There were some significant difference in pH, temperature stability and kinetic parameters. The presence of high starch concentration resulted in higher thermostability in recombinant enzyme than the wild type. The recombinant enzyme was more stable at higher temperature and lower pH, with lower $K_m$ for coupling reaction using cellobiose and cyclodextrins as substrates.

• 한국전산구조공학회논문집
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• 제18권2호
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• pp.169-179
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• 2005

탄성 및 비탄성좌굴 고유치해석법을 이용하여 강절프레임의 보-기둥부재의 유효좌굴길이를 산정하는 개선된 방법을 제시한다. 이를 위하여 먼저 설계기준에 제시된 압축재의 내하력 곡선식으로부터 접선계수이론(tangent modulus theory)에 근거하여 세장비-접선계수(tangent modulus), 응력-변형률 곡선식을 유도한다. 이때 안정함수를 이용하여 보-기둥요소의 접선강성행렬을 얻고, 비탄성 좌굴 고유치해석법을 제시하며 이를 이용하여 유효좌굴길이를 산정하는 방법을 제시한다. 해석예제를 통하여 강절프레임에 탄성 및 비탄성좌굴해석법에 의한 유효좌굴길이 비교결과를 제시하고, 매개변수 연구 결과를 제시한다.

• 한국소음진동공학회논문집
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• 제15권4호
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• pp.492-498
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• 2005

A linear motor damper based on the linear motor principle is developed to suppress structural vibration. This paper deals with the design, analysis, and manufacture of the linear motor damper. It is designed to be able to move the auxiliary mass of 1500kg, up to $\pm250mm$ stroke. The control algorithm was designed based on LQG control logic with acceleration feedback. Through performance tests, it was confirmed that the developed hybrid mass damper has reliable feasibility as a control device for structural control. In addition, the linear motor damper is more economical than both hydraulic and electric motor driving mass damper with respect to simple structure and low maintenance cost. A series of performance tests of the linear motor damper system were carried out on the full-scale steel frame structure in UNISON Corporation. Through the performance tests, it was confirmed that acceleration levels are reduced down 10dB for first mode of structure

• 한국강구조학회 논문집
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• 제18권6호
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• pp.707-716
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• 2006

본 논문에서는 고등해석과 유전자 알고리즘을 이용한 반강접 강뼈대 구조물의 직접설계시스템의 최적화를 수행하였다. 고등해석은 접합부의 비선형, 기하학적 비선형 및 재료적 비선형을 고려한다. 기하학적 비선형은 안정함수를 사용하여 고려하였으며, 재료적 비선형은 CRC 접선 탄성계수와 포물선 함수를 사용함으로서 고려하였다. 접합부의 비선형은 Kishi와 Chen이 제안한 3가지 매개변수를 가지는 파워모델을 사용하여 고려하였다. 최적화 기법으로는 유전자 알고리즘을 사용하였다. 목적함수는 구조물의 중량을 사용하였으며, 제약조건식은 구조시스템의 하중-저항능력, 처짐, 층간 수평변위 및 연성요구 조건을 고려하였다. 제안된 방법에 의한 설계결과를 기존의 방법들과 비교하였다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2008년도 제39회 하계학술대회
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• pp.924-925
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• 2008

There are many types of grid-connected inverter controllers, PI controller based is the most popular methods. But, a common PI control is produced zero-steady state error and phase delay in sinusoidal reference. Synchronous reference frame or DQ transform based controller is capable for reducing both of zero-steady state error and phase delay is. But DQ transform based controller has cross-coupling component which difficult to analyze the system in single phase model. In this paper, to obtained single phase model of the system, DQ transform based controller is analyzed in two techniques. The first is by neglecting cross-coupling. The second is eliminated cross-coupling component by decoupling method. By these two techniques, single phase model is obtained. Then, the single phase model is analyzed to evaluate its performance in stability and frequency response, through Root Locus and Bode diagram, respectively. MATLAB and PSIM simulation is used to verify the analysis. Simulation result is shown; cross-coupling component has no significant influent to the controller.

• 대한치과교정학회지
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• 제16권2호
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• pp.43-51
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• 1986

The highly accurate laser holographic interferemotry method was used to determine in what way low-magnitude forces during maxillary expansion are transmitted to the entire maxillary complex and its surrounding structures. The experiments were carried out on a dryed human skull which had a perfectly preserved, normally aligned maxillary dental arch and intact alveolar process. The skull was fixed within a constructed metal frame which ensured maximal stability of the object. The optical equipment and the object were mounted on antivibration table. Interferograms were taken on the lateral and frontal sides of the maxillary complex, using the 10mW He-Ne laser and the double-exposure method. Analysis of the fringe pattern on the recorded object surface was performed by graphically determining the deformation curves related to the bony surface in selected horizontal and vertical planes. On the basis of this study, the following conclusions can be drawn: 1. The density of the interference fringes was gradually increased with the degree of expansion force. 2. Mechanical reactions on the maxillary complex, circummaxillary sutures, and surrounding bones were clearly visible, even with the lowest loading degree. 3. The amount of bone displacement was greater in application of the force after $90^{\circ}$ turn than in initial application of the same force. 4. The direction of interference fringes on the bony surface was similar at all loading degrees.

• KIEE International Transaction on Electrical Machinery and Energy Conversion Systems
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• 제5B권4호
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• pp.350-357
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• 2005

This paper presents a mechanical sensorless vector-controlled system with parameter identification by the aid of image processor. Based on the flux observer and the model reference adaptive system method, the proposed sensorless system includes rotor speed estimation and stator resistance identification using flux errors. Since the mathematical model of this system is constructed in a synchronously rotating reference frame, a linear model is easily derived for analyzing the system stability, including motor operating state and parameter variations. Because it is difficult to identify rotor resistance simultaneously while estimating rotor speed, a low-accuracy image processor is used to measure the mechanical axis position for calculating the rotor speed at a steady-state operation. The rotor resistance is identified by the error between the estimated speed using the estimated flux and the calculated speed using the image processor. Finally, the validity of this proposed system has been proven through experimentation.

• 한국화재소방학회논문지
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• 제27권3호
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• pp.72-79
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• 2013

최근 건축물은 재료의 고강도화 및 단면형상 최적화를 바탕으로 초고층화, 장대화 및 복합화 추세로 발달하고 있으나, 화재 위험성에 의한 구조적 거동에 관한 연구는 매우 미진한 실정이다. 특히 고강도 구조용 강재로 구성된 강구조 건축물의 구조부재에 대한 화재 노출 시 거동평가를 위한 기초적인 자료조차 거의 없는 실정이다. 따라서 본 연구에서는 고층 강구조 건축물에 활용되고 있는 고강도 구조용 강재의 고온 시 구조적 내력 평가를 위한 기계적 특성과 열적 특성 자료 도출을 목적으로 항복강도, 탄성계수 그리고 열전도율 등을 측정하고, 이를 일반 구조용 강재의 특성과 비교분석을 통하여 상대적 안전성을 평가한다.

• Journal of Welding and Joining
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• 제33권1호
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• pp.49-53
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• 2015

An experimental study was conducted to investigate the effect of carbon nanotubes on the zinc corrosion resistance of sealing layer formed on the Tungsten Carbide spray coating. Using the nanotubes, a sealing agent in the form of solid-liquid suspensions was made and applied to the surface of spray coating. A series of experiments, consisted of three stages such as preparation of test piece, molten-pot immersion test, and evaluation of micro structure, were undertaken to demonstrate complicated interaction existing between zinc ions and sealing layer containing the nanotubes. Experimental results showed newly developed sealing layer were less susceptible to corrosion and thus coated layer was well protected even in the case of 10 days exposure. Comparison of the micro structure after molten pot test also indicated that carbon nanotubes still remained in the matrix and organized more reliable frame work constituted with boron nitride and chromium compound. It was revealed that carbon nanotubes in the sealing layer played positive role to enhance zinc corrosion resistance in the perspective of both fibrous structure and inherent chemical stability.

• 한국구조물진단유지관리공학회 논문집
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• 제6권3호
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• pp.129-138
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• 2002

The purpose of this study is to propose the nonlinear analysis method which combines the nonlinear incremental method with the layered method to solve the problems due to the geometric and the material nonlinearities. As numerical analysis models, the reinforced concrete simple beam and the steel arch frame are used to verify the algorithm of the proposed nonlinear method. The results are gotten from the computation procedures. According to the results of this study, the fracture pattern of the beam according to the ratio of tensile steel and the strength of the concrete and the steel can be estimated by the proposed method. Therefore, the load-deflection curve of structure can be, exactly, depicted by the proposed method. Also, the rupture load, the site and the depth of crack of the beam can analytically be checked by the proposed method. In this respect, the proposed method contributes for the solving the stability problem of the actual structure.