• Journal of Korean Society of Steel Construction
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• v.16 no.2 s.69
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• pp.235-245
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• 2004

The advantages of composite construction are now well understood in terms of structural economy, good performance in service, and ease of construction. However, these conventional composite construction systems have some problems in application to steel framed buildings due to their large depth. So, in this study we executed an experimental test with the "Slim Floor"system which could reduce the overall depth of composite beam. Slim Floor system is a method of steel frame multi-story building construction in which the structural depth of each floor is minimized by incorporating the steel floor beams within the depth of the concrete floor slab. Presented herein is an experimental study that focuses on the flexural behaviour of the partially connected slim floor system with asymmetric steel beams encased in composite concrete slabs. Eight full-scale specimens were constructed and tested in this study with different steel beam height, slab width, with or without shear connection and concrete topping thickness. Observations from experiments indicated that the degree of shear connection without additional shear connection was $0.53{\sim}0.95$ times that of the full shear connection due to inherent mechnical and chemical bond stress.

• BMB Reports
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• v.42 no.10
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• pp.673-678
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• 2009

The cDNA of alcohol dehydrogenase (PgADH) was isolated and characterized from the leaf of Panax ginseng. The cDNA had an open reading frame of 801 bp and a deduced amino acid sequence of 266 residues. The calculated molecular mass of the mature protein is approximately 29 kDa with a predicated isoelectric point of 6.84. Homology analysis revealed that the deduced amino acid of PgADH shares a high degree of homology with the short-chain ADH proteins of other plants. Genomic DNA hybridization analysis indicated that PgADH represents a multi-gene family. The expression of PgADH under various environmental stresses was analyzed at different time points using real-time PCR. ABA, SA and especially JA (80-fold) significantly induced PgADH expression within 24 h of treatment. The positive responses of PgADH to abiotic stimuli suggest that ginseng ADH may protect against hormone-related environmental stresses.

• Journal of Korean Society of Steel Construction
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• v.24 no.6
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• pp.725-734
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• 2012

A new modular pier system using concrete filled circular steel tubes was suggested to realize modular bridge substructures for accelerated bridge construction. Structural details and connection details were proposed by connection multiple concrete filled tubes (CFT) for standardized products of fabrication, delivery and erection. Static tests were performed for the modular pier with suggested details under lateral load conditions for weak and strong axes. Due to the eccentricity by the bracing system, the modular pier showed 5.23 times higher flexural stiffness and 6 times greater flexural strength from the test. It is proper for the rational design to evaluate stress and deformation by frame modeling of the modular CFT pier. Structural capacity of the pier can be obtained by adjusting the spacing of the CFT columns. Design recommendations were derived from the test.

• Fisheries and Aquatic Sciences
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• v.14 no.1
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• pp.43-54
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• 2011

Gene structure of copper/zinc-superoxide dismutase (Cu/Zn-SOD; sod1) was characterized in Hemibarbus mylodon (Teleostei; Cypriniformes), an endangered freshwater fish species in Korean peninsula. Full-length cDNA of H. mylodon SOD1 consisted of a 796-bp open reading frame sequence encoding 154 amino acids, and the deduced polypeptide sequence shared high sequence homology with other orthologs, particularly with regard to metal-coordinating ligands. Genomic structure of the H. mylodon sod1 gene (hmsod1; 1,911 bp from the ATG start codon to the stop codon) was typical quinquepartite (i.e., five exons interrupted by four introns); the lengths of the exons were similar among species belonging to various taxonomic positions. The molecular phylogeny inferred from sod1 genes in the teleost lineage was in accordance with the conventional taxonomic assumptions. 5'-flanking upstream region of hmsod1, obtained using the genome walking method, contained typical TATA and CAAT boxes. It also showed various transcription factor binding motifs that may be potentially involved in stress/immune response (e.g., sites for activating proteins or nuclear factor kappa B) or metabolism of xenobiotic compounds (e.g., xenobiotic response element; XRE). The hmsod1 transcripts were ubiquitously detected among tissues, with the liver and spleen showing the highest and lowest expression, respectively. An experimental challenge with Edwardsiella tarda revealed significant upregulation of the hmsod1 in kidney (4.3-fold) and spleen (3.1-fold), based on a real-time RT-PCR assay. Information on the molecular characteristics of this key antioxidant enzyme gene could be a useful basis for a biomarker-based assay to understand cellular stresses in this endangered fish species.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.11a
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• pp.89-92
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• 2008

The seismic behavior of the lightly reinforced concrete frames (LRCFs) was controlled by the nonductile behavior of the critical regions. These critical regions require retrofit to improve the seismic behavior of the lightly reinforced concrete frames. Critical column end regions must be retrofit to increase the global ductility capacity. The objective of this research is to evaluate structural strengthening performance of lightly reinforced concrete frame with Strain hardening cement composite(SHCC) experimentally. The experimental investigation consisted of a cyclic load tests on 1/3-scale models of precast infill walls. Reinforcement detail of infill wall was variables in the experiment. The experimental results, as expected, show that the multiple crack pattern, strength, ductility and energy dissipation capacity are superior for specimen with SHCC infill wall due to bridging of fibers and stress redistribution in cement matrix.

• Journal of the Earthquake Engineering Society of Korea
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• v.14 no.4
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• pp.29-36
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• 2010

To investigate the cyclic characteristics of exterior joints in RC frame buildings which are typically used after 1988, 70% scaled T-shaped beam-column subassemblies were designed and tested with a displacement control that is composed of 9 steps, until 3.5% story drift was reached. Axial forces are applied to columns during the experiment to simulate a real situation. The results show that the non-seismic detailed specimens failed before reaching 0.85% story drift, and their strengths are less than 0.85 times the nominal flexural strength which beam or columns should reach. The relationship of principal stress and story drift of exterior joints is similar to the one that Priestly proposed.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.9 no.1
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• pp.33-40
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• 1989

In the design of reinforced concrete framed structures, which consist of various design variables, the objective and the constraint functions are formulated in complicated forms. Usually iterative methods have been used to optimize the design variables. In this paper, multilevel formulation is adopted, and design variables are selected in reduced numbers at each level, to reduce the iterative cycle and to accelerate the convergence rate. At level 1, elastic analysis is performed to get the upper and lower bounds of the redistributed design moments due to inelastic behavior of the frame. Then the design moments are taken as design variables and optimized at level 2, and the sizing variables are optimized at level 3. The optimization of redistributed moments is performed using the design sensitivity obtained at the level 2, and force approximation technique is used to reflect the variation of design variables in the lower level to the upper level. The design variables are selected in reduced numbers at each level, and the optimization formulation is simplified effectively. A cost function is taken as the objective function, and the constraints of the stress of the structures are derived from BSI CP 110 following limit state theory. Numerical examples are included to prove the effectiveness of the developed algorithm.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.13 no.2
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• pp.41-49
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• 1993

This paper presents a geometrically non-linear and elastoplastic F.E. formulation using a total Lagrangian approach for the two dimensional isoparametric curved beam elements. The beam element is derived by using plane stress elements. The basic element geometry is constructed using the coordinates of the nodes on the element center line and the nodal point normals. The element displacement field is described using two translations of the node on the center line and a rotation about the axes normal to the plane containing the center line of the element. The layered approach is used for the elastoplastic analysis of the plane framed structure with the arbitrary cross section. The iterative load or displacement incremental method for non-linear finite element analysis of the frame structure is used. Numerical examples are presented to demonstrate the behavior and the accuracy of the proposed beam element for geometric and elastoplastic non-linear applications. Comparisons made with present theory and other published data show that tilt' beam element products accurate results with good convergence characteristics.

• Journal of the Korea Concrete Institute
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• v.25 no.5
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• pp.529-537
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• 2013

The aim of the current study is to develop a nonlinear isoparametric layered frame finite element (FE) analysis of FRP strengthened reinforced concrete (RC) beam or column members by a force-based FE formulation. In sections, concrete is modeled in the triaxial stress-strain relationship state and the FRP sheet is modeled as layered composite materials in two-dimension. The element stiffness matrix derived by the force-based FE has the force-interpolation functions without assuming the displacement shape functions. A lateral load test of RC column strengthened by GFRP sheets was analyzed by the developed force-based FE model. From comparative studies of the experimental and analysis results, it was shown to compare with the stiffness FE method that the force-based FE analysis could give more accurate predictions in the overall lateral load-deflection response as well as in nonlinear deformations and damages in the column plastic hinge region.

• BMB Reports
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• v.34 no.4
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• pp.379-384
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• 2001

The cDNA encoding ribosomal protein was identified from a cDNA library of Schizosaccharomyces pombe. The nucleotide sequence of the 548 by cDNA clone reveals an open reading frame, which encodes a putative protein of 166 amino acids with a molecular mass of 18.3 kDa. The amino acid sequence of the S. pombe L11 protein is highly homologous with those of rat and fruit, while it is clearly less similar to those of prokaryotic counterparts. The 1,044 by upstream sequence, and the region encoding N-terminal 7 amino acids of the genomic DNA were fused into the promoterless $\beta$-galactosidase gene of the shuttle vector YEp357 in order to generate the fusion plasmid pHY L11. Synthesis of $\beta$-galactosidase from the fusion plasmid varied according to the growth curve. It decreased significantly in the growth-arrested yeast cells that were treated with aluminum chloride and mercuric chloride. However, it was enhanced by treatments with cadmium chloride ($2.5\;{\mu}M$), zinc chloride ($2.5\;{\mu}M$), and hydrogen peroxide (0.5 mM). This indicates that the expression of the L,11 gene could be induced by oxidative stress.