To ascertain the effect of over-expressed maize calreticulin in tomato plant on tobamovirus movement in addition to validating potentiality of the gene (ZmCRT) as a means for the virus-resistance resource, four ZmCRT-expressing homozygous lines were generated from the T0 plants as using an Agrobacterium-mediated transformation, nucleic acid analyses, and a conventional breeding method. Of them, a line was subjected to the bioassay for tolerances to tobacco mosaic virus-U1 (TMV-U1) and tomato mosaic virus (ToMV) followed by RT-PCR and a chlorophyll fluorescence quenching analyses. Both transgenic plants transcribing ZmCRT and wild-type plants showed no symptom by 20 days after viruses inoculation, however the photosystem II quantum yield parameter measured from the upper leaves of ToMV-inoculated plants revealed that ZmCRT transgenic plants have higher photosynthetic ability than wild-type ones at that time, which indirectly implies that over-expressed ZmCRT product acts as a barrier to the cell-to-cell and/or systemic movement of ToMV. Moreover, ZmCRT transgenic plants showed remarkably longer shoot length than wild-type ones in 40 days after TMV-U1 or ToMV inoculation each, which might be resulted from higher photosynthetic ability during the phase not yet showing any external symptoms. Collectively, over-expressed ZmCRT protein in tomato plants is able to interrupt the systemic movement of infected TMV-U1 and ToMV even though not perfect.
Sevim, Baris;Altunisik, Ahmet Can;Bayraktar, Alemdar
Computers and Concrete
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v.10
no.3
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pp.277-294
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2012
The aim of the study is to determine the modal parameters of a prototype damaged arch dam by operational modal analysis (OMA) method for some damage scenarios. For this purpose, a prototype arch dam-reservoir-foundation model is constructed under laboratory conditions. Ambient vibration tests on the arch dam model are performed to identify the modal parameters such as natural frequency, mode shape and damping ratio. The tests are conducted for four test-case scenarios: an undamaged dam with empty reservoir, two different damaged dams with empty reservoirs, and a damaged dam with full reservoir. Loading simulating random impact effects is applied on the dam to crack. Cracks and fractures occurred at the middle of the upper part of the dams and distributed through the abutments. Sensitivity accelerometers are placed on the dams' crests to collect signals for measurements. Operational modal analysis software processes the signals collected from the ambient vibration tests, and enhanced frequency domain decomposition and stochastic subspace identification techniques are used to estimate modal parameters of the dams. The modal parameters are obtained to establish a basis for comparison of the results of two techniques for each damage case. Results show that approximately 35-40% difference exists between the natural frequencies obtained from Case 1 and Case 4. The natural frequencies of the dam considerably decrease with increasing cracks. However, observation shows that the filled reservoir slightly affected modal parameters of the dam after severe cracking. The mode shapes obtained are symmetrical and anti-symmetrical. Apparently, mode shapes in Case 1 represent the probable responses of arch dams more accurately. Also, damping ratio show an increase when cracking increases.
This paper presents research on methods for the reduction of forklifts' noise level for the increased comfort and safety of its operator. A cooling fan with a high air volume flow rate installed in the forklift acts as an important design parameter which efficiently cools the heat exchanger system, helping to transfer internal heat from the engine room to the outdoors with both transmitted and diffracted opening noises. The cooling fan contributes significantly to both the forklift's emitted sound power and the operator room's noise level, thereby necessitating research on the forklift's reduction of acoustic power level and transmission. A noise analysis for various fan models with a biomimetic design based on eagle-wing geometry was conducted. In addition to the acoustic power generation, the aerodynamic performance of the cooling blade is also strongly influenced by the design of airfoil distribution, thereby requiring optimization. The cooling fans were fabricated and installed in the forklift in order to check the efficacy of the forklift engine's cooling, and the final version of the fan was measured for its ability to lower acoustic power level and cool the engine room. This study explains the aerodynamic and acoustic features of the designed fans with the use of BEM analysis and forklift test results.
High-speed turbomachinery implements gas foil bearings (GFBs) due to their distinctive advantages, such as high efficiency, lesser part count, and lower weight. This paper provides the test results of the static structural stiffnesses and loss factors of bump-type foil thrust bearings with increasing preload and bearing deflection. The focus of the current work is to experimentally quantify variability in structural stiffnesses and loss factors among the four test thrust bearings with identical design values and material of the bump and top foil geometries using the same (open-source) fabrication method. A simple test setup, using a rigidly mounted non-rotating shaft and thrust disk, measures the bearing bump deflections with increasing static loads on the test bearing. The inner and outer diameters of the test bearings are 41 mm and 81 mm, respectively. The loss factor, best-representing energy dissipation in the test bearings, is estimated from the area inside the local hysteresis loop of the load versus the bearing deflection curve. The measurements show that structural stiffnesses and loss factors of the test bearings significantly rely on applied preloads and bearing deflections. Local structural stiffnesses of the test bearings increase with applied preloads but decrease with bearing deflections. Changes of loss factors are less sensitive to applied preloads and bearing deflections compared to those of structural stiffnesses. Up to 35% variability in static load structural stiffnesses is found between bearings, while up to 30% variability in loss factors is found between bearings.
Esen, Ismail;Alazwari, Mashhour A.;Eltaher, Mohamed A;Abdelrahman, Alaa A.
Steel and Composite Structures
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v.42
no.6
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pp.805-826
/
2022
The free and live load-forced vibration behaviour of porous functionally graded (PFG) higher order nanobeams in the thermal and magnetic fields is investigated comprehensively through this work in the framework of nonlocal strain gradient theory (NLSGT). The porosity effects on the dynamic behaviour of FG nanobeams is investigated using four different porosity distribution models. These models are exploited; uniform, symmetrical, condensed upward, and condensed downward distributions. The material characteristics gradation in the thickness direction is estimated using the power-law. The magnetic field effect is incorporated using Maxwell's equations. The third order shear deformation beam theory is adopted to incorporate the shear deformation effect. The Hamilton principle is adopted to derive the coupled thermomagnetic dynamic equations of motion of the whole system and the associated boundary conditions. Navier method is used to derive the analytical solution of the governing equations. The developed methodology is verified and compared with the available results in the literature and good agreement is observed. Parametric studies are conducted to show effects of porosity parameter; porosity distribution, temperature rise, magnetic field intensity, material gradation index, non-classical parameters, and the applied moving load velocity on the vibration behavior of nanobeams. It has been showed that all the analyzed conditions have significant effects on the dynamic behavior of the nanobeams. Additionally, it has been observed that the negative effects of moving load, porosity and thermal load on the nanobeam dynamics can be reduced by the effect of the force induced from the directed magnetic field or can be kept within certain desired design limits by controlling the intensity of the magnetic field.
Lightweight concrete is a superior material due to its light weight and high strength. There however remain significant lacunae in engineering knowledge with regards to shear failure of lightweight fiber reinforced concrete beams. The main aim of the present study is to investigate the optimum usage of steel fibers in lightweight fiber reinforced concrete (LWFRC). Multi-scale finite element model calibrated with experimental results is developed to study the effect of steel fibers on the mechanical properties of LWFRC beams. To decrease the amount of steel fibers, it is preferred to reinforce only the middle section of the LWFRC beams, where the flexural stresses are higher. For numerical simulation, a multi-scale finite element model was developed. The cement matrix was modeled as homogeneous and uniform material and both steel fibers and lightweight coarse aggregates were randomly distributed within the matrix. Considering more realistic assumptions, the bonding between fibers and cement matrix was considered with the Cohesive Zone Model (CZM) and its parameters were determined using the model update method. Furthermore, conformity of Load-Crack Mouth Opening Displacement (CMOD) curves obtained from numerical modeling and experimental test results of notched beams under center-point loading tests were investigated. Validating the finite element model results with experimental tests, the effects of fibers' volume fraction, and the length of the reinforced middle section, on flexural and residual strengths of LWFRC, were studied. Results indicate that using steel fibers in a specified length of the concrete beam with high flexural stresses, and considerable savings can be achieved in using steel fibers. Reducing the length of the reinforced middle section from 50 to 30 cm in specimens containing 10 kg/m3 of steel fibers, resulting in a considerable decrease of the used steel fibers by four times, whereas only a 7% reduction in bearing capacity was observed. Therefore, determining an appropriate length of the reinforced middle section is an essential parameter in reducing fibers, usage leading to more affordable construction costs.
Ismail M. Mudhaffar;Abdelbaki Chikh;Abdelouahed Tounsi;Mohammed A. Al-Osta;Mesfer M. Al-Zahrani;Salah U. Al-Dulaijan
Structural Engineering and Mechanics
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v.86
no.2
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pp.167-180
/
2023
This work applies a four-known quasi-3D shear deformation theory to investigate the bending behavior of a functionally graded plate resting on a viscoelastic foundation and subjected to hygro-thermo-mechanical loading. The theory utilizes a hyperbolic shape function to predict the transverse shear stress, and the transverse stretching effect of the plate is considered. The principle of virtual displacement is applied to obtain the governing differential equations, and the Navier method, which comprises an exponential term, is used to obtain the solution. Novel to the current study, the impact of the viscoelastic foundation model, which includes a time-dependent viscosity parameter in addition to Winkler's and Pasternak parameters, is carefully investigated. Numerical examples are presented to validate the theory. A parametric study is conducted to study the effect of the damping coefficient, the linear and nonlinear loadings, the power-law index, and the plate width-tothickness ratio on the plate bending response. The results show that the presence of the viscoelastic foundation causes an 18% decrease in the plate deflection and about a 10% increase in transverse shear stresses under both linear and nonlinear loading conditions. Additionally, nonlinear loading causes a one-and-a-half times increase in horizontal stresses and a nearly two-times increase in normal transverse stresses compared to linear loading. Based on the article's findings, it can be concluded that the viscosity effect plays a significant role in the bending response of plates in hygrothermal environments. Hence it shall be considered in the design.
Journal of Korea Society of Digital Industry and Information Management
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v.18
no.1
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pp.79-91
/
2022
In this study, the effects of self-efficacy, perceived usefulness, perceived ease of use, and depression on college students' academic persistence in the COVID-19 epidemic and the resulting non-face-to-face education situation were identified as mediating effects on learning satisfaction. In the second semester of 2020, a survey was conducted on students enrolled in a four-year university in Daegu and the data were statistically analyzed. The path coefficient was estimated by the Smart PLS bootstrap method and the significance of the path coefficient was verified. The Sobel Test was conducted to verify the mediating effect of academic continuity intention as a parameter. The research results can be summarized as follows. First, it was found that self-efficacy and perceived usefulness had a significant influence in the relationship with learning satisfaction. Second, the relationship between learning satisfaction and academic continuity intention was found to have a significant influence. Third, depression and ease of use did not show any significant influence in the relationship between learning satisfaction. Finally, a Sobel Test was conducted to verify the mediating effect of academic continuity intention with self-efficacy, usefulness, ease of use, and depression as independent variables and learning satisfaction as parameters. As a result of both regression analyses, it was found that β values decreased, and learning satisfaction had a mediating effect. As a result of this study, it is suggested that research to increase learner satisfaction and develop various contents to increase the effectiveness of education that can increase self-efficacy and perceived usefulness should be conducted in parallel. I think this study can be used as basic data in establishing measures to continue studying for college students in natural disaster situations or psychological crisis situations called COVID-19.
Magazine of the Korean Society of Agricultural Engineers
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v.29
no.4
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pp.59-72
/
1987
Although long-term runoff analysis is important as much as flood analysis in the design of water works, the technological level of the former is relatively lower than that of the latter. In this respect, the precise estimation model for the volume of successive runoff should he developed as soon as possible. Up to now, in Korea, Gajiyama's formula has been widely used in long-term runoff analysis, which has many problems in applying in real situation. On the other hand, in flood analysis, unit hydrograph method has been exclusively used. Therefore, this study aims at trying to apply unit hydrograph method in long-term runoff analysis for the betterment of its estimation. Four test catchment areas were selected ; Maesan area in Namlum river as a representative area of Han river system, Cheongju area in Musim river as one of Geum river system, Hwasun area in Hwasun river as one of Yongsan river system, and Supyung area in Geum river as one of Nakdong river system. In the analysis of unit hydrograph, seperation of effective rainfall was carried out firstly. Considering that effective rainfall and moisture condition of catchrnent area are inside and outside of a phenomenon respectively and the latter is not considered in the analysis, Initial base flow(qb)was selected as an index of moisture condition. At the same time, basic equation(Eq.7) was established, in which qb can take a role as a parameter in relating between cumulative rainfall(P) and cumulative loss of rainfall(Ld). Based on the above equation, computer program for estimation model of qbwas seperately developed according to the range of qb, Developed model was applied to measured hydrographs and hyetographs for total 10 years in 4 test areas and effective rainfall was estimated. Estimation precision of model was checked as shown in Tab- 6 and Fig.8. In the next stage, based on the estimated effective rainfall(R) and runoff(Qd), a runoff distribution ratio was calculated for each teat area using by computerised least square method and used in making unit hydrographs in each test area. Significance of induced hydrographs was tested by checking the relative errors between estimated and measured runoff volume(Tab-9, 10). According to the results, runoff estimation error by unit hydrograph itself was merely 2 or 3 %, but other 2 or 3 % of error proved to be transferred error in the seperation of effective rainfall. In this study, special attentioning point is that, in spite of different river systems and forest conditions of test areas, standardized unit hydrographs for them have very similar curve shape, which can be explained by having similar catchinent characteristics such as stream length, catchinent area, slope, and vegetation intensity. That fact should be treated as important factor ingeneralization of unit hydrograph method.
Bae, Jong In;Kim, Jeong Won;Lee, Haeng Bok;Kim, Myung-Whun
Korean Journal of Optics and Photonics
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v.33
no.5
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pp.218-229
/
2022
We have investigated reliable inspection methods for finding the defects generated during the manufacturing process of lightweight, large-aperture satellite telescope mirrors using silicon carbide, and we have measured the basic physical properties of the mirrors. We applied the advanced ceramic material (ACM) method, a combined method using liquid-silicon penetration sintering and chemical vapor deposition for the carbon molded body, to manufacture four SiC mirrors of different sizes and shapes. We have provided the defect standards for the reflectors systematically by classifying the defects according to the size and shape of the mirrors, and have suggested effective nondestructive methods for mirror surface inspection and internal defect detection. In addition, we have analyzed the measurements of 14 physical parameters (including density, modulus of elasticity, specific heat, and heat-transfer coefficient) that are required to design the mirrors and to predict the mechanical and thermal stability of the final products. In particular, we have studied the detailed measurement methods and results for the elastic modulus, thermal expansion coefficient, and flexural strength to improve the reliability of mechanical property tests.
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