Browse > Article
http://dx.doi.org/10.5010/JPB.2010.37.4.567

Suppression of tobamovirus movement toward upper leaves in the tomato plant over-expressing a maize calreticulin  

Han, Jeung-Sul (Department of Ecological Environment Conservation, College of Ecology & Environment Science, Kyungpook National University)
Publication Information
Journal of Plant Biotechnology / v.37, no.4, 2010 , pp. 567-573 More about this Journal
Abstract
To ascertain the effect of over-expressed maize calreticulin in tomato plant on tobamovirus movement in addition to validating potentiality of the gene (ZmCRT) as a means for the virus-resistance resource, four ZmCRT-expressing homozygous lines were generated from the T0 plants as using an Agrobacterium-mediated transformation, nucleic acid analyses, and a conventional breeding method. Of them, a line was subjected to the bioassay for tolerances to tobacco mosaic virus-U1 (TMV-U1) and tomato mosaic virus (ToMV) followed by RT-PCR and a chlorophyll fluorescence quenching analyses. Both transgenic plants transcribing ZmCRT and wild-type plants showed no symptom by 20 days after viruses inoculation, however the photosystem II quantum yield parameter measured from the upper leaves of ToMV-inoculated plants revealed that ZmCRT transgenic plants have higher photosynthetic ability than wild-type ones at that time, which indirectly implies that over-expressed ZmCRT product acts as a barrier to the cell-to-cell and/or systemic movement of ToMV. Moreover, ZmCRT transgenic plants showed remarkably longer shoot length than wild-type ones in 40 days after TMV-U1 or ToMV inoculation each, which might be resulted from higher photosynthetic ability during the phase not yet showing any external symptoms. Collectively, over-expressed ZmCRT protein in tomato plants is able to interrupt the systemic movement of infected TMV-U1 and ToMV even though not perfect.
Keywords
tomato; calreticulin; genetic transformation; tobamovirus; systemic movement; chlorophyll fluorescence quenching analysis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Shaterian J, Georges F, Hussain A, Waterer D, Jong HD, Tanino KK (2005) Root to shoot communication and abscisic acid in calreticulin (CR) gene expression and salt-stress tolerance in grafted diploid potato clones. Environ Exp Bot 53:323-332   DOI
2 Stam M, Mol JM, Kooter JM (1997) The silence of genes in transgenic plants. Annal Bot 79:3-12   DOI
3 Vieira J, Messing J (1987) Production of single-stranded plasmid DNA. Meth Enzym 153:3-11   DOI
4 Wolf S, Millatiner A (2000) Effect of tobacco mosaic virus movement protein on photosynthesis in transgenic tobacco plants. J Plant Physiol 156:253-258   DOI   ScienceOn
5 Wyatt SE, Tsou PL, Robertson D (2002) Expression of the high capacity calcium-binding domain of calreticulin increases bioavailable calcium stores in plants. Transgenic Res 11:1-10   DOI
6 Mlynárová L, Jansen RC, Conner AJ, Stiekema WJ, Nap JP (1995) The MAR-mediated reduction in position effects can be uncoupled from copy number-dependent expression in transgenic plants. Plant Cell 7:599-609   DOI
7 Park S, Cheng NH, Pittman JK, Yoo KS, Park J, Smith RH, Hirschi KD (2005) Increased calcium levels and prolonged shelf life in tomatoes expressing Arabidopsis $H^{+}/Ca^{2+}$ transporters. Plant Physiol 139:1194-1206   DOI
8 Park SH, Morris JL, Park JE, Hirschi KD, Smith RH (2003) Effcient and genotype-independent Agrobacterium-mediated tomato transformation. J Plant Physiol 160:1253-1257   DOI
9 Perez-Bueno ML, Ciscato M, vande Ven M, Garcia-Luque I, Valcke R, Baron M (2006) Imaging viral infection: studies on Nicotiana benthamiana plants infected with the pepper mild mottle tobamovirus. Photosynth Res 90:111-123   DOI
10 Persson S, Rosenquist M, Svensson K, Galvão R, Boss WF, Sommarin M (2003) Phylogenetic analyses and expression studies reveal two distinct groups of calreticulin isoforms in higher plants. Plant Physiol 133:1385-1396   DOI
11 Persson S, Wyatt SE, Love J, Thompson WF, Robertson D, Boss WF (2001) The $Ca^{2+}$ status of the endoplasmic reticulum is altered by induction of calreticulin expression in transgenic plants. Plant Physiol 126:1092-1104   DOI
12 Fras A, Maluszynska J (2003) Regeneration of diploid and tetraploid plants of Arabidopsis thaliana via callus. Acta Biologica Cracoviensia Series Botanica 45:145-152
13 Pineda M, Soukupová J, Matouš, Nedbal L, Barón M (2008) Conventional and combinatorial chlorophyll fluorescence imaging of tobamovirus-infected plants. Photosynthetica 46:441-451   DOI
14 Saito Y, Ihara Y, Leach MR, Cohen-Dole MF, Williams DB (1999) Calreticulin functions in vitro as a molecular chaperone for both glycosylated and non-glycosylated proteins. EMBO J 18:6718-6729   DOI
15 Ding B, Haudenshield JS, Hull RJ, Wolf S, Beachy RN, Lucas WJ (1992) Secondary plasmodesmata are specific sites of localization of the tobacco mosaic virus movement protein in transgenic tobacco plants. Plant Cell 4:915-928   DOI
16 Genty B, Briantais JM, Baker NR (1989) The relationship between quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta 990:87-92   DOI   ScienceOn
17 Han JS, Oh DG, Mok IG, Park HG, Kim CK (2004) Efficient plant regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria Standl.). Plant Cell Rep 23:291-296   DOI
18 Hebert DN, Foellmer B, Helenius A (1996) Calnexin and calreticulin promote folding, delay oligomerization and suppress degradation of influenza hemagglutinin in microsomes. EMBO J 15:2961-2968
19 Holsters M, De Waele D, Depicker A, Messens E, Van Montagu M, Schell J (1978) Transfection and transformation of A. tumefaciens. Mol Gen Genet 163:181-187   DOI
20 Kitajima H, Butler WL (1975) Quenching of chlorophyll fluorescence and primary photochemistry in chloroplasts by dibromothymoquinone. Biochim Biophys Acta 376:105-115   DOI
21 Brunt A, Crabtree K, Dallwitz M, Gibbs A, Watson L, Zurcher E (1997) Plant viruses online: descriptions and lists from the VIDE database. http://biology.anu.edu.au/Groups/MES/vide/
22 Laporte C, Vetter G, Loudes A-M, Robinson DG, Hillmer S, Stussi-Garaud C, Ritzenthaler C (2003) Involvement of the secretory pathway and the cytoskeleton in intracellular targeting and tubule assembly of Grapevine fanleaf virus movement protein in tobacco BY-2 cells. Plant Cell 15:2058-2075   DOI
23 Lee LY, Kononov ME, Bassuner B, Frame BR, Wang K, Gelvin SB (2007) Novel plant transformation vectors containing the superpromoter. Plant Physiol 145:1294-1300   DOI
24 Miernyk JA (1999) Protein folding in the plant cell. Plant Physiol 121:695-703   DOI
25 Bulk RW, Loffler HJM, Lindhout WH, Koornneef M (1990) Somaclonal variation in tomato: effect of explants source and a comparison with chemical mutagenesis. Theor Appl Genet 80:817-825   DOI
26 Chen MH, Sheng J, Hind G, Handa AK, Citovsky V (2000) Interaction between the tobacco mosaic virus movement protein and host cell pectin methylesterases is required for viral cellto- cell movement. EMBO J 19:913-920   DOI
27 Chen MH, Tian G-W, Gafni Y, Citovsky V (2005) Effects of calreticulin on viral cell-to-cell movement. Plant Physiol 138:1866-1876   DOI
28 Christov I, Stefanov D, Velinov T, Goltsev V, Georgieva K, Abracheva P, Genova Y, Christov N (2007) The symptomless leaf infection with grapevine leafroll associated virus 3 in grown in vitro plants as a simple model system for investigation of viral effects on photosynthesis. J Plant Physiol 164:1124-1133   DOI
29 Chung MY, Han JS, Giovannoni J, Liu Y, Kim CK, Lim KB, Chung JD (2010) Modest calcium increase in tomatoes expressing a variant of Arabidopsis cation/$H^{+}$ antiporter. Plant Biotechnol Rep 4:15-21   DOI
30 Citovsky V, Wong ML, Shaw A, Prasad BVV, Zambryski PC (1992) Visualization and characterization of tobacco mosaic virus movement protein binding to single-stranded nucleic acids. Plant Cell 4:397-411   DOI
31 Dan H, Imaseki H, Wasteneys GO, Kazama H (2003) Ethylene stimulates endoreduplication but inhibits cytokinesis in cucumber hypocotyls epidermis. Plant Physiol 133:1726-1731   DOI
32 De Buck S, Peck I, De Wilde C, Marjanac G, Nolf J, De Paepe A, Depicker A (2007) Generation of single-copy T-DNA transformants in Arabidopsis by the CRE/loxP recombinationmediated resolution system. Plant Physiol 145:1171-1182   DOI
33 Bilger W, Bjorkman O (1990) Role of the xanthophylls cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and phothosynthesis in leaves of Hedera canariensis. Photosynth Res 25:173-185   DOI
34 Akesson A, Persson S, Love J, Boss WF, Widell S, Sommarin M (2005) Overexpression of the-$Ca^{2+}$-binding protein calreticulin in the endoplasmic reticulum improves growth of tobacco cell suspensions (Nicotiana tabacum) in high-$Ca^{2+}$ medium. Physiol Plant 123:92-99   DOI
35 Baluška F, Samaj J, Napier R (1999) Maize calreticulin localizes preferentially to plasmodesmata in root apex. Plant J 19:481-488   DOI