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Detection of Microcystin Synthetic Cyanobacteria and Variation of Intracellular Microcystin Synthesis Using by eDNA and eRNA in Freshwater Ecocystem (담수환경에서 eDNA와 eRNA를 이용한 Microcystin 합성 남조류 탐색 및 세포 내 Microcystin 생합성 활성 변화)

  • Keonhee Kim;Chaehong Park;Hyeonjin Cho;Daeryul Kwon;Soon-Jin Hwang
    • Korean Journal of Ecology and Environment
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    • v.56 no.1
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    • pp.1-13
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    • 2023
  • Targeting Microcystin (MC), which is most abundantly detected in the North-Han River water area, we analyzed the relationship between the MC biosynthesis gene (mcyA gene), cyanobacteria cell density, and MC concentration, derived an RNA-MC conversion formula, and derived the cyanobacteria. The concentration of MC present in cells was predicted. In the North-Han River waters, the mcyA gene was found mainly at downstream sites of the North-Han River after Muk-Hyeon Stream junction, and higher copy numbers were found on average than other sites. In the Uiam Lake waters upstream of the North-Han River, the mcyA gene copy number increased at the Kong-Ji Stream point, and after September, the mcyA gene copy number decreased throughout the North-Han River waters. The expression of the mcyA gene was concentrated in the short period of summer due to the spatio-temporal difference between upstream and downstream water bodies. The mcyA gene expression level was not only highly correlated with MC concentration, but also correlated with the cell density of Microcystis aeruginosa and Dolichospermum circinale, which are known to biosynthesize MC. Six conversion formulas derived based on the RNA-MC relationship showed statistical significance (p<0.05) and exhibited high correlation coefficients (r) of 0.9 or higher. The expression level of MC biosynthesis gene present in eRNA determines the synthesis of cyanotoxin substances in water, quickly quantifies gene activity, and can be fully utilized for early warning of MC development.

Methods of Estimating Growing Degree Days to Predict Growth Duration in Maize (옥수수의 생육기간 예측을 위한 Growing Degree Days의 계산방법)

  • Jong, Seung-Keun;Lee, Suk-Soon;Park, Keun-Yong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.2
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    • pp.186-194
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    • 1986
  • In an attempt to find better ways to relate growth with temperature and to estimate maturity differences in corn (Zea mays L.), various formulas of computing Growing Degree Days (GDD) were evaluated. Utilizing data from 17 plantings of a single cross, Suweon 19, over a 3 year period, 24 different methods of computing GDD were compared for their ability to reduce variations over different plantings. The best equation was to compute GDD with a base temperature of 10$^{\circ}C$ and an optimum of 30$^{\circ}C$. The excess temperature above 30$^{\circ}C$ was subtracted to account for high temperature stress. GDDs required for emergence and silking of Suweon 19 were 64${\pm}$12$^{\circ}$ and 794${\pm}$19$^{\circ}$, respectively. Based on these GDD values, emergence and silking dates could be estimated with a variation less than 3 days. The observed and estimated number of days from planting to emergence and silking were not significantly different.

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Variation of Earth Pressure Acting on Cut-and-Cover Tunnel Lining with Settlement of Backfill (되메움토의 침하에 따른 개착식 터널 라이닝에 작용하는 토압의 변화)

  • Bautista F.E.;Park Lee-Keun;Im Jong-Chul;Lee Young-Nam
    • Journal of the Korean Geotechnical Society
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    • v.22 no.6
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    • pp.27-40
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    • 2006
  • Damage of cut-and-cover tunnel lining can be attributed to physical and mechanical factors. Physical factors include material property, reinforcement corrosion, etc. while mechanical factors include underground water pressure, vehicle loads, etc. This study is limited to the modeling of rigid circular cut and cover tunnel constructed at a depth of $1.0{\sim}1.5D$ in loose sandy ground and subjected to a vibration frequency of 100 Hz. In this study, only damages due to mechanical factors in the form of additional loads were considered. Among the different types of additional, excessive earth pressure acting on the cut-and-cover tunnel lining is considered as one of the major factors that induce deformation and damage of tunnels after the construction is completed. Excessive earth pressure may be attributed to insufficient compaction, consolidation due to self-weight of backfill soil, precipitation and vibration caused by traffic. Laboratory tunnel model tests were performed in order to determine the earth pressure acting on the tunnel lining and to investigate the applicability of existing earth pressure formulas. Based on the difference in the monitored and computed earth pressure, a factor of safety was recommended. Soil deformation mechanism around the tunnel was also presented using the picture analysis method.

Reliability Management - From the Perspective of Quality Management Engineer Test (신뢰성관리 - 품질경영기사 시험의 측면에서)

  • Jaiwook Baik
    • Industry Promotion Research
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    • v.8 no.2
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    • pp.37-43
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    • 2023
  • Sampling In this study, we examined the problems and their improvement plans associated with the reliability management sector in quality management engineer test conducted in Korea. First of all, there seems to be a problem in that the terminology is not unified and some techniques essential for reliability analysis are not included. We also looked at quality and reliability tests performed in foreign countries (especially USA) that Koreans often acquire. In particular, it can be seen that the CRE test almost overlaps with the contents of the reliability management engineer test in Korea. However, while the USA is an open book test, Korea is not, so the problem is that there are too many formulas to memorize on the part of the test takers. In addition, the analysis of the data is done manually without using computer software. If the test were an open book test like the CRE test in USA, it will be a test that can go beyond fragmentary knowledge and check whether test takers have the essential elements in reliability management. Lastly, if we adopt re-certification system through education and work within a certain period of time, as in USA, it will be a qualification test suitable for modern people living in a flood of information.

Analysis of mathematics test structures and tasks in Abitur (독일 아비투어(Abitur)의 수학시험 체제 및 문항 분석)

  • Kim, Seong-kyeong;Lee, Miyoung
    • The Mathematical Education
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    • v.61 no.2
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    • pp.287-303
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    • 2022
  • The purpose of this study is to draw implications for the improvement in the CSAT by analyzing structures and tasks in the Abitur. To this end, it analyzes the mathematics test system with a focus on the basic and advanced level examination systems, the operator, the using technology, and mathematical formulas. And the characteristics of tasks in the 2021 Abitur were analyzed. As a result of the analysis, first, Germany evaluates whether students have the competency emphasized in the curriculum at Abitur. Second, Germany, which emphasizes the proper use of technology, utilizes both tasks that use technology and those that do not in the Abitur. Third, the Abitur consists of most of the tasks using promised operators and uses various types of operators to present various types of questions to evaluate competence. Fourth, the Abitur includes not only simple structured items consisting of 2-3 subtasks but also tasks dealing in depth with a single situation centered on a big idea. Finally, mathematical justification and proof play an important role in the Abitur. Based on this, some specific measures for improving the CSAT were suggested.

Influence of Water-Cement Ratios and Curing Conditions on the Diffusion Characteristics of Chloride Ion in Concrete (콘크리트의 염소이온 확산특성에 미치는 물-시멘트비 및 양생조건의 영향)

  • Bae, Su-Ho;Lee, Kwang-Myong;Kim, Jee-Sang;Jung, Sang-Hwa
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.26 no.4A
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    • pp.753-759
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    • 2006
  • To predict service life of concrete structures exposed to chloride attack, surface chloride concentration, diffusion coefficient of chloride ion, and chloride corrosion threshold value in concrete, are used as important factors. Of these, as the diffusion coefficient of chloride ion for concrete is strongly influenced by concrete quality and environmental conditions of structures and may significantly change the service life of structures, it is considered as the most important factor for service life prediction. The qualitative factors affecting the penetration and diffusion of chloride ion into concrete are water-cement (W/C) ratio, age, curing conditions, chloride ion concentration of given environment, wet and dry conditions, etc. In this paper the influence of W/C ratio and curing conditions on the diffusion characteristics of chloride ion in concrete was investigated through the chloride ion diffusion test. In the test, the voltages passing through the diffusion cell were measured by accelerated test method using potential difference, and then with the consideration of IR drop ratio the diffusion coefficient of chloride ion for concrete with different W/C ratios were estimated by Andrade's model. Furthermore, under different curing conditions formulas for the estimation of the diffusion coefficient of chloride ion have been proposed by the regression analysis considering the effect of W/C ratio and age.

Evaluating the contribution of calculation components to the uncertainty of standardized precipitation index using a linear mixed model (선형혼합모형을 활용한 표준강수지수 계산 인자들의 불확실성에 대한 기여도 평가)

  • Shin, Ji Yae;Lee, Baesung;Yoon, Hyeon-Cheol;Kwon, Hyun-Han;Kim, Tae-Woong
    • Journal of Korea Water Resources Association
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    • v.56 no.8
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    • pp.509-520
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    • 2023
  • Various drought indices are widely used for assessing drought conditions which are affected by many factors such as precipitation, soil moisture, and runoff. The values of drought indices varies depending on hydro-meteorological data and calculation formulas, and the judgment of the drought condition may also vary. This study selected four calculation components such as precipitation data length, accumulation period, probability distribution function, and parameter estimation method as the sources of uncertainty in the calculation of standardized precipitation index (SPI), and evaluated their contributions to the uncertainty using root mean square error (RMSE) and linear mixed model (LMM). The RMSE estimated the overall errors in the SPI calculation, and the LMM was used to quantify the uncertainty contribution of each factor. The results showed that as the accumulation period increased and the data period extended, the RMSEs decreased. The comparison of relative uncertainty using LMM indicated that the sample size had the greatest impact on the SPI calculation. In addition, as sample size increased, the relative uncertainty related to the sample size used for SPI calculation decreased and the relative uncertainty associated with accumulation period and parameter estimation increased. In conclusion, to reduce the uncertainty in the SPI calculation, it is essential to collect long-term data first, followed by the appropriate selection of probability distribution models and parameter estimation methods that represent well the data characteristics.

Prediction accuracy of incisal points in determining occlusal plane of digital complete dentures

  • Kenta Kashiwazaki;Yuriko Komagamine;Sahaprom Namano;Ji-Man Park;Maiko Iwaki;Shunsuke Minakuchi;Manabu, Kanazawa
    • The Journal of Advanced Prosthodontics
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    • v.15 no.6
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    • pp.281-289
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    • 2023
  • PURPOSE. This study aimed to predict the positional coordinates of incisor points from the scan data of conventional complete dentures and verify their accuracy. MATERIALS AND METHODS. The standard triangulated language (STL) data of the scanned 100 pairs of complete upper and lower dentures were imported into the computer-aided design software from which the position coordinates of the points corresponding to each landmark of the jaw were obtained. The x, y, and z coordinates of the incisor point (XP, YP, and ZP) were obtained from the maxillary and mandibular landmark coordinates using regression or calculation formulas, and the accuracy was verified to determine the deviation between the measured and predicted coordinate values. YP was obtained in two ways using the hamularincisive-papilla plane (HIP) and facial measurements. Multiple regression analysis was used to predict ZP. The root mean squared error (RMSE) values were used to verify the accuracy of the XP and YP. The RMSE value was obtained after crossvalidation using the remaining 30 cases of denture STL data to verify the accuracy of ZP. RESULTS. The RMSE was 2.22 for predicting XP. When predicting YP, the RMSE of the method using the HIP plane and facial measurements was 3.18 and 0.73, respectively. Cross-validation revealed the RMSE to be 1.53. CONCLUSION. YP and ZP could be predicted from anatomical landmarks of the maxillary and mandibular edentulous jaw, suggesting that YP could be predicted with better accuracy with the addition of the position of the lower border of the upper lip.

Dose Reduction Method for Chest CT using a Combination of Examination Condition Control and Iterative Reconstruction (검사 조건 제어와 반복 재구성의 조합을 이용한 흉부 CT의 선량 저감화 방안)

  • Sang-Hyun Kim
    • Journal of the Korean Society of Radiology
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    • v.17 no.7
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    • pp.1025-1031
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    • 2023
  • We aimed to evaluate the radiation dose and image quality by changing the Scout view voltage in low-dose chest CT (LDCT) and applying scan parameters such as AEC (auto exposure control) and ASIR (adaptive statistical iterative reconstruction) to find the optimal protocol. Scout view voltage was varied at 80, 100, 120, 140 kV and after measuring the dose 5 times using the existing low-dose chest CT protocol, the appropriate kV was selected for the study using the Dose report provided by the equipment. After taking a basic LDCT shot at 120 kV, 30 mAs, ASIR 50% was applied to this condition. Signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) were assessed by measuring Background noise (B/N). For dose comparison, CTDIvol and DLP provided by the equipment were compared and analyzed using the formulas. The results indicated that the protocol of scout 140 + LDCT + ASIR 50 + AEC reduced radiation exposure and improved image quality compared to traditional LDCT, providing an optimal protocol. As demonstrated in the experiment, LDCT screenings for asymptomatic normal individuals are crucial, as they involve concerns over excessive radiation exposure per examination. Therefore, applying appropriate parameters is important, and it is expected to contribute positively to the public health in future LDCT based health screenings.

A Study on Back Analysis Settlement Prediction of Soft Ground Using Numerical Analysis and Measurement Data (수치해석과 계측데이터를 이용한 연약지반의 역해석 침하 예측에 관한 연구)

  • Sangju Jeon;Hyeok Seo;Daehyeon Kim
    • Journal of the Korean Geosynthetics Society
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    • v.23 no.2
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    • pp.9-17
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    • 2024
  • When constructing on soft ground, managing ground settlement and safety is crucial. However, there often exists a significant disparity between the actual behavior of the ground and the design plans. In this study, we aimed to compare and analyze the difference between the predicted settlement based on theoretical formulas and the measured settlement during construction, in order to predict settlement. For this purpose, we analyzed settlement data from 18 construction sites. The results indicated that the back analysis settlement values were similar to the measured settlement values, whereas the design settlement values were significantly higher compared to the measured settlement values. Specifically, the design settlement values were 1.2 to 1.4 times higher than those derived from back analysis using measured values. The RMSE analysis revealed a value of 0.6212m for the design settlement and 0.1697m for the back analysis settlement. The difference between the back analysis settlement and the measured settlement was more than 70% lower than the difference between the design settlement and the measured settlement. This indicates that the back analysis settlement values exhibit lower error rates compared to the design settlement values.