• Title/Summary/Keyword: Forming effects

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Development of Predictive Growth Model of Listeria monocytogenes Using Mathematical Quantitative Assessment Model (수학적 정량평가모델을 이용한 Listeria monocytogenes의 성장 예측모델의 개발)

  • Moon, Sung-Yang;Woo, Gun-Jo;Shin, Il-Shik
    • Korean Journal of Food Science and Technology
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    • v.37 no.2
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    • pp.194-198
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    • 2005
  • Growth curves of Listeria monocytogenes in modified surimi-based imitation crab (MIC) broth were obtained by measuring cell concentration in MIC broth at different culture conditions [initial cell numbers, $1.0{\times}10^{2},\;1.0{\times}10^{3}\;and\;1.0{\times}10^{4}$, colony forming unit (CFU)/mL; temperature, 15, 20, 25, 37, and $40^{\circ}C$] and applied to Gompertz model to determine microbial growth indicators, maximum specific growth rate constant (k), lag time (LT), and generation time (GT). Maximum specific growth rate of L. monocytogenes increased rapidly with increasing temperature and reached maximum at $37^{\circ}C$, whereas LT and GT decreased with increasing temperature and reached minimum at $37^{\circ}C$. Initial cell number had no effect on k, LT, and GT (p > 0.05). Polynomial and square root models were developed to express combined effects of temperature and initial cell number using Gauss-Newton Algorism. Relative coefficients of experimental k and predicted k of polynomial and square root models were 0.92 and 0.95, respectively, based on response surface model. Results indicate L. monocytogenes growth was mainly affected by temperature and square root model was more effective than polynomial model for growth prediction.

Microbiological Characteristics of Gamma Irradiated and Low-Salted Fermented Squid (감마선 조사된 저염 오징어젓갈 발효의 미생물균총 특성)

  • Kim, Dong-Ho;Kim, Jae-Hun;Yook, Hong-Sun;Ahn, Hyun-Joo;Kim, Jung-Ok;Sohn, Cheon-Bae;Byun, Myung-Woo
    • Korean Journal of Food Science and Technology
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    • v.31 no.6
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    • pp.1619-1627
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    • 1999
  • Microbiological characteristics of gamma irradiated low salt squid Jeot-gal were examined. Following the fermentation periods, total bacterial cell, Lactobacillus spp., Staphylococcus spp., Streptococcus spp., Pseudomonas spp. and yeast cell number were counted on their selective media and some acid forming bacteria and Pseudomonas spp. were identified. As the gamma irradiation dose increased, the microbial density of early fermentation phase was reduced and the growth rate was delayed. The repression effects on microbiological growth by gamma irradiation were to be higher as salt concentration increased. Adequate conditions of salt concentration and gamma irradiation for low-salt squid Jeot-gal preparation were 10% and 10 kGy, respectively. Lactobacillus sp. 2, Micrococcus varians and Streptococcus sp. I were isolated from 5% salt containing squid Jeot-gal, and Micrococcus morrhuae was from 20% only while Lactobacillus plantarum and Lactobacillus brevis were widespread. Lactobacillus brevis, Pediococcus halophilus and Pseudomonas diminuta were sensitive and Lactobacillus plantarum, Micrococcus morrhuae and Pseudomonas sp. 3 were resistant to gamma irradiation. The diversity of microflora decreased as salt concentration decreased and gamma irradiation dose increased.

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Development of Predictive Growth Model of Vibrio parahaemolyticus Using Mathematical Quantitative Model (수학적 정량평가모델을 이용한 Vibrio parahaemolyticus의 성장 예측모델의 개발)

  • Moon, Sung-Yang;Chang, Tae-Eun;Woo, Gun-Jo;Shin, Il-Shik
    • Korean Journal of Food Science and Technology
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    • v.36 no.2
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    • pp.349-354
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    • 2004
  • Predictive growth model of Vibrio parahaemolyticus in modified surimi-based imitation crab broth was investigated. Growth curves of V. parahaemolyticus were obtained by measuring cell concentration in culture broth under different conditions ($Initial\;cell\;level,\;1{\times}10^{2},\;1{\times}10^{3},\;and\;1{\times}10^{4}\;colony\;forming\;unit\;(CFU)/mL$; temperature, 15, 25 37, and $40^{\circ}C$; pH 6, 7, and 8) and applying them to Gompertz model. Microbial growth indicators, maximum specific growth rate (k), lag time (LT), and generation time (GT), were calculated from Gompertz model. Maximum specific growth rate (k) of V. parahaemolyticus increased with increasing temperature, reaching maximum rate at $37^{\circ}C$. LT and GT were also the shortest at $37^{\circ}C$. pH and initial cell number did not influence k, LT, and GT values significantly (p>0.05). Polynomial model, $k=a{\cdot}\exp(-0.5{\cdot}((T-T_{max}/b)^{2}+((pH-pH_{max)/c^{2}))$, and square root model, ${\sqrt{k}\;0.06(T-9.55)[1-\exp(0.07(T-49.98))]$, were developed to express combination effects of temperature and pH under each initial cell number using Gauss-Newton Algorism of Sigma plot 7.0 (SPSS Inc.). Relative coefficients between experimental k and k Predicted by polynomial model were 0.966, 0.979, and 0.965, respectively, at initial cell numbers of $1{\times}10^{2},\;1{\times}10^{3},\;and\;1{\times}10^{4}CFU/mL$, while that between experimental k and k Predicted by square root model was 0.977. Results revealed growth of V. parahaemolyticus was mainly affected by temperature, and square root model showing effect of temperature was more credible than polynomial model for prediction of V. parahaemolyticus growth.

Effects of The Types of the Profile Pictures and the Types of Messages on the Impression Formation of the Twitter Account Owner (TAO) (트위터 프로필 사진 유형과 메시지 유형이 트위터 계정소유자 (TAO)의 인상형성에 미치는 영향 -트위터 계정 소유자(TAO)의 온라인 자기 제시(Self-presentation) 요소와 인상 형성 간의 상관관계 연구)

  • Kim, Ah-Reum;Park, Mi-Na;Jeon, Dae-Won;Kang, Mi-Ri;Kong, Hye-Jin;Gu, Yoo-Ri;Jin, Min-Soo;Kim, Joo-Han
    • Journal of the HCI Society of Korea
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    • v.6 no.2
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    • pp.1-9
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    • 2011
  • Increasing communication through social media forming impression becomes more important. In this research, how three stimulus can build the impression of Twitter Account Owner(TAO) such as the types of the profile pictures, the types of timeline messages and the number of followers. We analysed the feelings of respondents when they face TAO's formal picture and informal picture, informative message and normal message, the number of followers. We, in addition, measured the willingness of respondents whether they want to develop their relationship online and offline as well. As a result, informative message draws positive reliability and makes respondent want to build deeper relationship with TAO at online. Respondent answered TAO's informal picture was more likable than formal picture and more reliable when informative message and formal picture were provided together. Our study shows that there are actual differences at attitudes of respondents by the types of the profile pictures and the types of timeline messages. Thus, we can conclude that TAOs can modify their impressions at online as they hope to.

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Investigation of Corrosion Mechanism by Analyses of Spent Chromia Refractory fvom a Coal Gasifier (석탄 가스화기에서의 크로미아 내화물 분석을 통한 화학적 침식 기구 규명)

  • Kim Han Bom;Oh Myongsook
    • Journal of Energy Engineering
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    • v.13 no.4
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    • pp.281-290
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    • 2004
  • Spent refractories from a coal gasifier after 1000 hours of operation were analyzed for crystalline phases, chemical composition and microstructures as a function of slag penetration depth, and the slag corrosion mechanism was determined. The chemical corrosion of chromia refractory occurred via reaction between Cr$_2$O$_3$ of the refractory and FeO and A1$_2$O$_3$ in the slag. The FeO reacted with Cr$_2$O$_3$ at the slare/refractory interface and formed FeCr$_2$O$_4$. After all FeO were consumed, Al in the penetrating slag substituted Cr in Cr$_2$O$_3$, forming (Al, Cr)$_2$O$_3$, at the edges of the particle, which were broken to form fragments rich in Al. The corrosion resistance of Cr$_2$O$_3$ varied with the particle size and the extent of sintering, and the higher resistance was observed in the larger and more sintered particles. There was no chemical change in ZrO$_2$, but showed the effects of physical corrosion: the grain boundaries became more wavy, and ZrO$_2$ grains were split in the corroded area. The slag penetration depth increased in the refractory samples farther down from the feed nozzles.

Investigation on the Electrical Characteristics of mc-Si Wafer and Solar Cell with a Textured Surface by RIE (플라즈마기반 표면 Texturing 공정에 따른 다결정 실리콘 웨이퍼 표면물성과 태양전지 동작특성 연구)

  • Park, Kwang-Mook;Jung, Jee-Hee;Bae, So-Ik;Choi, Si-Young;Lee, Myoung-Bok
    • Journal of the Korean Vacuum Society
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    • v.20 no.3
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    • pp.225-232
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    • 2011
  • Reactive ion etching (RIE) technique for maskless surface texturing of mc-silicon solar wafers has been applied and succeed in fabricating a grass-like black-silicon with an average reflectance of $4{\pm}1%$ in a wavelength range of 300~1,200 nm. In order to investigate the optimized texturing conditions for mass production of high quantum efficiency solar cell Surface characteristics such as the spatial distribution of average reflectance, micrscopic surface morphology and minority carrier lifetime were monitored for samples from saw-damaged $15.6{\times}15.6\;cm^2$ bare wafer to key-processed wafers as well as the mc-Si solar cells. We observed that RIE textured wafers reveal lower average reflectance along from center to edges by 1% and referred the origin to the non-uniform surface structures with a depth of 2 times deeper and half-maximum width of 3 times. Samples with anti-reflection coating after forming emitter layer also revealed longer minority carrier lifetime by 40% for the edge compared to wafer center due to size effects. As results, mc-Si solar cells with RIE-textured surface also revealed higher efficiency by 2% and better external quantum efficiency by 15% for edge positions with higher height.

Effects of Bioturbation of the Amphipod Monocorophium uenoi on the Vertical Distribution of Sulphides and Oxygen within Sulphides-Rich Sediments of Lake Sihwa (세가시육질꼬리옆새우(단각류)의 생물교란이 시화호 퇴적물 내 황화합물과 산소 농도의 수직적 분포에 미치는 영향)

  • Koo, Bon Joo;Kwon, Kae Kyeong;Shin, Sang-Ho;Je, Jong-Geel
    • Journal of Wetlands Research
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    • v.8 no.3
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    • pp.79-89
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    • 2006
  • The survival rate and tube number of Monocorophium uenoi, one of the dominant species in Lake Sihwa, was investigated under laboratory condition with salinity gradients (1, 5, 10, 15, 20, 25, 30, 35, 40‰). Another laboratory experiments were conducted to investigate the survival rate of Monocorophium uenoi in sulphide-rich sediment with oxic overlying water, and the effect of amphipod bioturbation on sulphide and oxygen profiles. The survival rate and tube-forming number of the amphipod were high in salinity range of 20 to 30‰. The amphipod survival rate was also high in sulphidic sediment when the water column was oxic. The amphipod bioturbation affected the oxygen and sulphide content in the sediment; oxygen conditions in the upper sediment layers were improved, and simultaneously the concentrations of sulphide were reduced. And their depth was clearly dependent on amphipod density. In Lake Sihwa, previously anoxic bottoms with sulphidic sediment during summer turn oxic with pycnocline disappearance during autumn. Amphipods seem to have the capability to quickly invade such areas, and their tubing of the sediment may play an important role in preparing the sediment for further recolonisation of other macrobenthos.

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Evaluation of Effective Dose and Exposure Level of Radon in Process Handling NORM (인산석고 취급공정에서의 라돈농도 및 유효선량 수준 평가)

  • Chung, Eun Kyo;Jang, Jae Kil;Kim, Jong Kyu;Kim, Joon Beom;Kwon, Jiwoon
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.28 no.3
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    • pp.283-291
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    • 2018
  • Objectives: To monitor the radon concentration level in plants that handle phosphorus rock and produce gypsum board and cement, and evaluate the effective dose considering the effect of radon exposure on the human body. Methods: Airborne radon concentrations were measured using alpha-track radon detectors (${\alpha}$-track, Rn-tech Co., Korea) and continuous monitors (Radon Sentinel 1030, Sun Nuclear Co., USA). Radon concentrations in the air were converted to radon doses using the following equation to evaluate the human effects due to radon. H (mSv/yr) = Radon gas concentration x Equilibrium factor x Occupancy factor x Dose conversion factor. The International Commission on Radiological Protection (ICRP) used $8nSv/(Bq{\cdot}hr/m^3)$ as the dose conversion factor in 2010, but raised it by a factor of four to $33nSv/(Bq{\cdot}hr/m^3)$ in 2017. Results: Radon concentrations and effective doses in fertilizer manufacturing process averaged $14.3(2.7)Bq/m^3$ ($2.0-551.3Bq/m^3$), 0.11-0.54 m㏜/yr depending on the advisory authority and recommendation year, respectively. Radon concentrations in the gypsum-board manufacturing process averaged $14.9Bq/m^3$ at material storage, $11.4Bq/m^3$ at burnability, $8.1Bq/m^3$ at mixing, $10.0Bq/m^3$ at forming, $8.9Bq/m^3$ at drying, $14.7Bq/m^3$ at cutting, and $10.5Bq/m^3$ at shipment. It was low because it did not use phosphate gypsum. Radon concentrations and effective doses in the cement manufacturing process were $23.2Bq/m^3$ in the stowage area, $20.2Bq/m^3$ in the hopper, $16.8Bq/m^3$ in the feeder and $11.9Bq/m^3$ in the cement mill, marking 0.12-0.63 m㏜/yr, respectively. Conclusions: Workers handling phosphorous gypsum directly or indirectly can be assessed as exposed to an annual average radon dose of 0.16 to 2.04 mSv or 0.010 to 0.102 WLM (Working Level Month).

Functional Understating of Fibroblastic Reticular Cell within Lymph Node Stroma (림프절 스토로마 내의 fibroblastic reticular cell의 기능 이해)

  • So, Deuk Won;Ryu, Sul Hwa;Lee, Jong-Hwan
    • Journal of Life Science
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    • v.23 no.11
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    • pp.1409-1414
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    • 2013
  • Lymph node (LN) is the sites where mature lymphocytes become stimulated to respond to invading pathogens in the body. Lymphocytes screen the surfaces of pathogen-carrying antigen-presenting cells for cognate antigens, while moving along stromal structural back bone. Fibroblastic reticular cells (FRC) is stromal cell forming the 3 dimensional structure networks of the T cell rich zones in LN, and provide a guidance path for immigrating T lymphocytes. In these cooperative environments, the cell to cell bidirectional interactions between FRC and T cells in LN are therefore essential to the normal functioning of these tissues. Not only do FRCs physically construct LN architecture but they are essential for regulating T cell biology within these domains. FRC interact closely with T lymphocytes, is providing scaffolds, secreting soluble factors including cytokine in which FRCs influence T cell immune response. More recently, FRC have been found to induce peripheral T cell tolerance and regulate the extent to which newly activated T cells proliferate within LN. Thus, FRC-T cell crosstalk has important consequences for regulating immune cell function within LN. In addition, FRC have profound effects on innate immune response by secreting anti-microbial peptides and complement, etc in the inflammatory milieu. In summary, we propose a model in which FRC engage in a bidirectional touch to increase the T cell biological efficiency between FRC and T cells. This collaborative feedback loop may help to maintain tissue function during inflammation response.

Recovery Pattern and Seasonal Dynamics of Kelp Species, Ecklonia cava Population Formed Following the Large-scale Disturbance (대규모 교란현상 후 형성된 대형갈조류 감태(Ecklonia cava) 개체군의 계절적 변동 및 회복 양상)

  • KIM, SANGIL;KANG, YUN HEE;KIM, TAE-HOON;PARK, SANG RUL
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.21 no.3
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    • pp.103-111
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    • 2016
  • Seasonal dynamics of kelp forest-forming algae, Ecklonia cava population formed following the large-scale disturbance by Typhoon 'Bolaven' in August 2012 were investigated in Jeju Island, Korea. Morphological characteristics, recruits density, mortality rate, total density and biomass were monitored bimonthly from June 2013 to June 2015. Total and longest blade lengths, and individual weight of E. cava showed distinct seasonal trends. Stipe length increased from winter to spring, but did not show increase or reduced from summer to autumn. This indicates that morphological characteristics of E. cava are mainly affected by the change of blades. The optimal temperature for E. cava growth was about $15-18^{\circ}C$ during winter to spring while the growths were inhibited at the water temperature above $20^{\circ}C$ during summer. E. cava exhibited very low recruitment during spring-summer. However, high recruitment was observed on April 2015 when canopy cover was very low due to low density. This indicates that recruitment of E. cava was controlled not by seasonal effects but by physical factors such as canopy and space. The mortality rate of juvenile plants was highest due to their unstable settlement. By June 2015, 34 months after the disturbances, E. cava was almost recovered to the pre-disturbance population size structure. These results suggest that recovery of kelp forest following the large-scale disturbance requires a considerable period of time (more than three years). This study should provide valuable ecological information on management, restoration and protection of kelp species.