• Journal of Korean Society of Forest Science
• /
• v.101 no.2
• /
• pp.305-308
• /
• 2012

The box tree pyralid, Glyphodes perspectalis, was reared on an artificial diet based on dried powder of box tree leaves, Buxus microphylla var. koreana, and INSECTA F-II (Nihon Nosan Co., Ltd) at a ratio of 3:7 (w:w) and analyzed in their developmental characteristics from $1^{st}$ to $4^{th}$ generation. The developmental period of larva reared on artificial diet was longer than that of larva reared on host plant. Significant difference in larval period was also observed between $1^{st}$ and other generation groups. Pupation rate was the highest in $4^{th}$ generation followed by $3^{rd}$, $2^{nd}$, $1^{st}$ and host plant group. Significant difference in pupal period was noted between $1^{st}$ and 3rd generation group. Emergence rate was the highest in $4^{th}$ generation group followed by $3^{rd}$, host plant, $1^{st}$ and $2^{nd}$ generation group. There is a significant difference in male and female size between $1^{st}$ and $4^{th}$ generation group. Our result indicated that stable rearing of box tree pyralid through the year is possible by artificial diet, and the best adaptation to artificial diet was achieved at $4^{th}$ generation.

• Journal of the Korean Wood Science and Technology
• /
• v.43 no.2
• /
• pp.238-247
• /
• 2015

Katsura tree (Cercidiphyllum japonicum Sieb. Et Zucc) leaves were collected, air-dried and extracted with 70% aqueous acetone, then concentrated and sequentially fractionated using n-hexane, $CH_2Cl_2$, EtOAc, and $H_2O$ to be freeze dried for antioxidant and ${\alpha}$-glucosidase inhibition activity tests. The antioxidant activity of the extracts was evaluated using DPPH (1,1-diphenyl 2-picrylhydrazyl) free radical scavenging assay. The test concentrations were adjusted to 500, 250, 125, 62.5, 31.25, 15.62, 7.81, 3.9, 1.95 and 0.97 ppm. The $H_2O$ and EtOAc fractions showed higher activities compared with the control, ${\alpha}$-tocopherol, at all concentrations. The crude fraction also gave better activity at the concentrations lower than 62.5 ppm. However, the nonpolar n-hexane and $CH_2Cl_2$ fractions gave prominently lower activities compared with the control at all concentrations. The $IC_{50}$ values of the crude, EtOAc, and $H_2O$ fractions exhibited 11.78, 4.29 and $9.80{\mu}g/m{\ell}$, respectively, compared with $12.08{\mu}g/m{\ell}$ of the control. But the n-hexane and $CH_2Cl_2$ fractions indicated 300 and $91.85{\mu}g/m{\ell}$ of $IC_{50}$, respectively. ${\alpha}$-Glucosidase inhibition activity was evaluated at the concentrations of 50, 25, 12.5, 6.3, 3.1, 1.6 and 0.8 ppm. The inhibition activities were increased according to as the increase of sample concentrations. However, the nonpolar n-hexane and $CH_2Cl_2$ fractions indicated very low inhibition activities compared with acarbose, a positive control. The EtOAc fraction showed very good capability as almost 100% compared with the control at the higher concentrations than 12.5 ppm and the crude fraction also indicated good potential as 95% and 100% at 25 and 50 ppm, respectively. The $H_2O$ fraction gave good inhibition value as 90% at 50 ppm although the value was lower than the control. These results showed that the polar fractions had better ${\alpha}$-glucosidase inhibition activities. The $IC_{50}$ values of the nonpolar fractions, n-hexane and $CH_2Cl_2$, showed very lower values as 468 and $103.6{\mu}g/m{\ell}$, respectively, than the control. ${\alpha}$-Glucosidase Inhibition Activity of the Extracts of Katsura Tree (Cercidiphyllum japonicum Sieb. Et Zucc) Leaves However, the polar fractions, crude, EtOAc and $H_2O$, showed 7.1, 3.7 and $13{\mu}g/m{\ell}$, respectively, indicating that these fractions can be used as natural bioresources for treating diabetes mellitus. Also ${\alpha}$-glucosidase inhibition activity had a positive correlation with antioxidant activity of the extracts.

• Korean Journal of Agricultural and Forest Meteorology
• /
• v.11 no.1
• /
• pp.3-12
• /
• 2009

Ozone tolerance of tree species was determined by standard index of physiological damages and biochemical defense responses under short-term ozone exposure. At the end of 150ppb $O_3$ fumigation, photosynthetic characteristics and antioxidative enzyme activities were analyzed in the leaves of five species(Koelreuteria paniculata, Firmiana simplex, Styrax japonica, Fraxinus rhynchophylla, Viburnum sargentii). Injury index was determined by the effect of ozone on photosynthetic parameters and malondialdehyde(MDA) content, and tolerance index was calculated using the rate of increase in superoxide dismutase(SOD), ascorbate-peroxidase(APX), glutathione reductase(GR) and catalase(CAT) activities. Apparent quantum yield(AQY), carboxylation efficiency(Ce) and photo-respiration rate(PR) decreased in the leaves of five species with increasing ozone exposure time. These parameters were considered as an appropriate indicator for stress evaluation. Antioxidative enzyme activities showed various results depending on the tree species, exposure time, and enzyme types. SOD activity of K. paniculata increased with ozone exposure time, and that of F. rhynchophylla increased only after 6 hours of ozone exposure. CAT activity of $O_3$-exposed F. simplex was lower than the control. Based on standard index, ozone tolerance ability of five species was determined as two tolerant species(F. rhynchophylla > K. paniculata) and three sensitive species(S. japonica > F. simplex > V. sargentii).

• Journal of Korean Society of Forest Science
• /
• v.89 no.1
• /
• pp.18-23
• /
• 2000

Leaves of various, 3 to 5-year-old Quercus hybrids were intermediate in size between their parental species. The petiole length was the smallest in the hybrids of Q. dentata ${\times}$ Q. crispula $F_1$ and was intermediate in the hybrids of Q. aliena ${\times}$ Q. serrata $F_1$ and Q. dentata ${\times}$ Q. aliena $F_1$ between their parents. The number of serration in hybrids was close to their mother tree's in most of crossing combinations. The serration depth and the ratio between longitudinal and transverse length of leaves were intermediate between the values of their parental species.

• Journal of Forest and Environmental Science
• /
• v.29 no.4
• /
• pp.282-291
• /
• 2013

Rapid loss in viability of neem (Azadirachta indica A. Juss.) seed is a major problem. Present effort was undertaken for developing a set pattern for assessing of viability and vigour in seed of various mother tree age of neem (Age I-06 years, Age II-15 years, Age III-25 years and Age IV->30 years old). Various viability test viz. triphenyle tetrazolium chloride test, electrical conductivity, excised embryo test, and germination test have been performed on seeds obtained from mother tree age classes. Inconsistency was observed with the TTC and EC test in germination of seed in laboratory as well as nursery. While various vigour tests viz. cold test, chemical stress test (methanol stress test), and accelerated ageing test alongwith ageing index, germination test (G%, MGT and GV) and various seedling growth parameters like seedling length (cm), number of leaves, collar diameter (cm), total biomass (g) alongwith mathematical indices i.e. vigour index, sturdiness quotient, volume index, quality index, root shoot ratio in nursery as well have been taken for study and showed better consistency. On the basis present study results of various viability and vigour test indicated that mother tree age class II performed better in comparison to others and it can be recommended for seed collection. Further it is also recommended that viability of neem seed may be assessed using various laboratory tests like excise embryo test and germination test (G%, MGT and GV) and vigour test may be taken preferably by cold germination test, chemical (methanol) stress test, accelerated ageing test in laboratory and germination alongwith various seedling growth parameters seedling length (cm), number of leaves, collar diameter (cm), total biomass (g) alongwith mathematical indices like Vigour Index, Sturdiness quotient, Volume Index, Quality index, root shoot ratio in nursery as discussed in this study.

• Journal of Forest and Environmental Science
• /
• v.36 no.1
• /
• pp.17-24
• /
• 2020

This study aimed to figure out and compare the increment of biomass by thinning intensity focused on the plantation of the two major coniferous species (Larix kaempferi and Pinus koraiensis) of South Korea. The inventory interval was three years under the effects of three types of thinning treatments; control (no thinning), light (20% thinning) and heavy (40% thinning). The results showed standing biomass increment of both species decreased as thinning intensity increased (heavylight>control). Meanwhile, the lowest of on-site biomass changes occurred in the control plot, and the greatest was in the heavy thinning plot because thinning was involved with leaving the felling residual biomass (leaves, branches and roots) on the site. According to the results from this short-term study, unthinned stands is preferable for maximizing standing biomass as well as carbon sequestration. However long-term investigation should be considered in order to see more clear results.

• Journal of the Korea Furniture Society
• /
• v.23 no.1
• /
• pp.95-102
• /
• 2012

The objective of this study was to conduct the species identification and tree-ring dating of Lotus Pedestal of the Amitabha Statue at Ssangbong-Temple in Hwasun. The six wood blocks used for the Lotus Pedestal were hard pines (Pinus spp.; diploxilon) except one piece which was ginkgo (Ginkgo biloba L.). The lotus leaves surrounding the pedestal body were also made of ginkgo. Tree-ring patterns of 3 blocks were synchronized and a 133 years chronology was made. The chronology was crossdated well with the master chronology of Japanese red pine in South Korea. It dated back to A. D. 1551~1683, i.e. the last ring dated A. D. 1683. Through the estimation of the number of sapwood rings removed during carving, the felling year was calculated A. D. $1704{\pm}10$. The calligraphic writing on the Pedestal indicated that this statue was made in A. D. 1694. Therefore, the accuracy of the tree-ring dating has been proven.

• Journal of the Korean Society of Safety
• /
• v.25 no.6
• /
• pp.210-215
• /
• 2010

In this study, when the forest fire occurred, in order to estimate greenhouse gas emissions, tree glow in Gangwon Youndong area, Juglans mandshurica, Alnus japonica, Acer palmatum of carbon dioxide and carbon monoxide emissions were about. Water content were measured before the experiment, Juglans mandshurica 196.24%, Alnus japonica 169.17% Acer palmatum 210.10% moisture content showed a big difference, Living leaves of Acer palmatum were a lot of moisture. Also, 50g weight of carbon dioxide on the Juglans mandshurica 53.3644g, Alnus japonica 49.4256g, was released about Acer palmatum 51.3394g, Juglans mandshurica living leaves were the most carbon dioxide emissions. Carbon monoxide emissions result, About weight 50g Juglans mandshurica 1.5329g, Alnus japonica 1.7189g, 2.5002g about Acer palmatum was released, Acer palmatum living leaves were the most carbon monoxide emissions.

• Journal of the Korean Wood Science and Technology
• /
• v.28 no.4
• /
• pp.17-24
• /
• 2000

The peroxidase activity was localized cytochemically to get an insight into its precise function in lignin biosynthesis. In this work, cerium chloride ($CeCl_3$) was used as a trapping agent for hydrogen peroxide ($H_2O_2$) generated from peroxidase. Seasonal variation of peroxidase activities in cambial region of Populus, Pinus, and Ginkgo was investigated at subcellular levels. Under transmission electron microscopy, electron dense deposits of cerium perhydroxide formed by reaction with $H_2O_2$ were observed in cambium and its immediate derivatives. The staining with $CeCl_3$ in cambium varied with growth seasons. The strongest $H_2O_2$ accumulation, regardless of tree species, appeared in May. Staining pattern of $CeCl_3$ in the cambium of poplar indicated that the production of peroxidase started in March before the opening of buds and reached the highest in May and then declined in August. Ginkgo and Pinus showed relatively late generation of $H_2O_2$ production when compared with Populus. Although Ginkgo and Pinus are classified into gymnosperms, however, the generation of peroxidase production and its duration was different from each other. Little staining appeared in all the tree samples collected in September before falling the leaves.

• Korean Journal of Ecology and Environment
• /
• v.51 no.4
• /
• pp.322-330
• /
• 2018

Herbivory is a major functional component of forest ecosystems, and herbivorous insects comprise about 25% of all insect species. Increasing plant diversity is related to herbivore abundance and diversity, which affects the level of leaf damage caused by insects. This study was conducted to identify plant-related variables such as plant diversity and number of leaves and density-related variables, basal area, and diameter at breast height (DBH) in a temperate forest of southern South Korea. To assess the level of leaf damage caused by leaf chewing insects, we set up two 0.1 ha plots in a temperate deciduous forest on Mt. Jirisan National Park. Plant richness differed between two sites: 16 species in 14 families(site 1) and 19 species in 15 families (site 2). Fisher's alpha index based on plant species richness and abundance resulted in 4.41 (site 1) and 6.57 (site 2). However, the sum of basal area of each site was higher in site 1 ($6.6m^2$) than site 2 ($3.7m^2$). The total surveyed leaves at two sites were 3,832 and 4,691, respectively and the damage leaves were 1,544 and 2,136, respectively. The mean leaf damage level was 11.2% (${\pm}1.76%$) in two study sites: the leaf damage level of the site 1 (11.99%) was significantly higher than site 2 (10.59%). Stepwise regression analysis showed that species diversity and evenness were the significant variables for leaf damages by chewing herbivores. NMDS ordination also identified that high tree density and low species diversity were the significant variables. This suggested that the level of damage was significantly higher in plots with low plant diversity and high tree density. In the future, we will investigate other guilds of herbivores such as sap-suckers, miners and gallers in temperate deciduous forests.