• Title/Summary/Keyword: Forensic sciences

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Application study of PCR additives to improve the split peaks in direct PCR

  • Kim, Joo-Young;Kim, Da-Hye;Park, Hyun-Chul;Jung, Ju Yeon;Jin, Gang-Nam;Hwang, In-Kwan;Kang, Pil-Won
    • Analytical Science and Technology
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    • v.32 no.4
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    • pp.155-162
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    • 2019
  • Analysis techniques using DNA profiling are widely used in various fields including forensic science and new technologies such as the Direct PCR amplification method are being developed continuously in order to acquire the DNA profiles efficiently. However, it has a limits such as non-specific amplification according to the quality of crime scene evidence samples. Especially, split peaks caused by excessive DNA samples are one of the important factors that could cause the debate to allow researchers to interpret the DNA profile results. In this study, we confirmed the occurrence rate of split peaks in each STR (short tandem repeats) locus of the $GlobalFiler^{TM}$ kit and investigated the possibility of improving the split peaks using several PCR additives such as DMSO (dimethylsulfoxide), $MgCl_2$, Betaine and Tween-20. As a result, we could make three groups according to the occurrence rate of split peaks in Direct PCR and it was confirmed that the ratio of split peaks could be reduced by DMSO (87.4 %), $MgCl_2$ (84.5 %) and Betaine (86.1 %), respectively. These results indicate that PCR additives such as DMSO, $MgCl_2$ and Betaine can be improve the split peaks in Direct PCR and thereby facilitate subsequently a successful DNA profile results.

On the Availability of Anti-Forensic Tools for Android Smartphones (안드로이드 스마트폰을 위한 앤티-포렌식 도구들의 활용성)

  • Moon, Phil-Joo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.8 no.6
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    • pp.855-861
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    • 2013
  • Smartphone is very useful for use in the real life through the improvement of computing power, faster data rate and the variety of applications. On the other hand, using the smartphone has been exposed to a lot of crime. Also, it occurs attempting to delete a data of smartphone memory by anti-forensic tools. In this paper, we investigate and analyze the anti-forensic tools used in the Android smartphone to study the characteristics and techniques of anti-forensic tools. In addition, experiments are performed to validate the availability of anti-forensic tools by the Oxygen Forensic Suite that is a commercial forensic tool.

A Case of Single-step Mutations at Two Short Tandem Repeat loci (D13S317 and DXS10148) among Three Generations of a Korean Family

  • Byeong Ju Youn;Kyungmyung Lee;Cho Hee Kim
    • Biomedical Science Letters
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    • v.28 no.4
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    • pp.327-333
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    • 2022
  • The DNA profiling of short tandem repeat (STR) markers is a powerful tool for forensic identification and forensic paternity testing. However, STR loci are susceptible to mutation that cause mismatches between parents and children when paternity is tested. Herein, we examined paternity disputes with 23 autosomal STR loci using two commercial human identification kits and revealed successive mismatches at the D13S317 locus across three generations of a Korean family. Additionally, we investigated 12 X-chromosomal STRs and discovered an inconsistency at the DXS10148 locus between the father and daughter of the same Korean family. Furthermore, we confirmed STR genotypes at the D13S317 and DXS10148 loci of the family using sequencing analysis. Consequently, we identified a successive single-step mutation at the D13S317 locus and one single-step mutation at the DXS10148 locus in three generations of the Korean family. Therefore, this case study may be useful for interpreting and understanding forensic paternity tests.

Evaluation of Rapid IgG4 Test for Diagnosis of Gnathostomiasis

  • Wang, Yue;Ma, An;Liu, Xiao-Long;Eamsobhana, Praphathip;Gan, Xiao-Xian
    • Parasites, Hosts and Diseases
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    • v.59 no.3
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    • pp.257-263
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    • 2021
  • Human gnathostomiasis is a parasitic disease caused by Gnathostoma nematode infection. A rapid, reliable, and practical immunoassay, named dot immuno-gold filtration assay (DIGFA), was developed to supporting clinical diagnosis of gnathostomiasis. The practical tool detected anti-Gnathostoma-specific IgG4 in human serum using crude extract of third-stage larvae as antigen. The result of the test was shown by anti-human IgG4 monoclonal antibody conjugated colloidal gold. The sensitivity and specificity of the test were both 100% for detection in human sera from patients with gnathostomiasis (13/13) and from healthy negative controls (50/50), respectively. Cross-reactivity with heterogonous serum samples from patients with other helminthiases ranged from 0 (trichinosis, paragonimiasis, clonorchiasis, schistosomiasis, and cysticercosis) to 25.0% (sparganosis), with an average of 6.3% (7/112). Moreover, specific IgG4 antibodies diminished at 6 months after treatment. This study showed that DIGFA for the detection of specific IgG4 in human sera could be a promising tool for the diagnosis of gnathostomiasis and useful for evaluating therapeutic effects.

Confidence Measure of Forensic Speaker Identification System According to Pitch Variances (과학수사용 화자 식별 시스템의 피치 차이에 따른 신뢰성 척도)

  • Kim, Min-Seok;Kim, Kyung-Wha;Yang, IL-Ho;Yu, Ha-Jin
    • Phonetics and Speech Sciences
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    • v.2 no.3
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    • pp.135-139
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    • 2010
  • Forensic speaker identification needs high accuracy and reliability. However, the current level of speaker identification does not reach its demand. Therefore, the confidence evaluation of results is one of the issues in forensic speaker identification. In this paper, we propose a new confidence measure of forensic speaker identification system. This is based on pitch differences between the registered utterances of the identified speaker and the test utterance. In the experiments, we evaluate this confidence measure by speech identification tasks on various environments. As the results, the proposed measure can be a good measure indicating if the result is reliable or not.

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Effectiveness of interference filter for photoluminescence observations: comparison with absorption filters

  • Lee, Wonyoung;Kim, Duke;Yu, Jeseol
    • Analytical Science and Technology
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    • v.34 no.5
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    • pp.225-230
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    • 2021
  • Currently, most photoluminescence reagents used in forensic science have short stokes shift, making it difficult to observe strong photoluminescence due to scattering light from forensic light sources when using filters that are not shieldable. However interference filters can be observed near monochromatic light, making them a better alternative to absorption filters. To verify practical applicability of interference filters, interference filters and three other types of previously frequently used absorption filters were observed using a variety of light source products, and transmission light was cross-compared. Interference filters have a lower slope value than absorption filters, and selectively show only the photoluminescence of reagents, regardless of the type of product from the forensic light source. In addition, tilting the angle of the filter surface for observation lowered the λcut-on, which could replace various types of absorption filters with a single interference filter.

A Study of Forensic Techniques and Tools on Smart Phone OS Types (Smart Phone OS별 포렌식 기술과 도구에 관한 연구)

  • Lee, Bo-Man;Park, Dea-Woo
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2010.10a
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    • pp.223-226
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    • 2010
  • iPhone and Smart Phone domestic markets are activated with domestic coming out of galaxy S together and the users are increasing. It follows hereupon and with forensic techniques for the cellular phone relation criminal evidence collection of data of existing the mobile forensic engineering research is necessary in about Smart Phone where it has become new techniques and WiFi etc. Convergence. It respects Smart Phone OS star forensic technique and a tool research, iPhone Apple MAC OS X and it researches galaxy S Google Android from the present paper. Smart it uses SYN methods with Phone OS star forensic techniques and it researches in order mobile forensic description below it observes it sees and forensic application methods computer forensic methods and the tools and it researches forensic techniques. The present paper research as IT powerful country will contribute in Smart Phone forensic technical advances of Korea.

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A study of the preparation And procedures by Smartphone Mobile Forensic evidence collection and analysis (스마트폰 모바일 포렌식 증거 수집 분석을 위한 준비사항 및 절차 연구)

  • Lee, Jae-Hyun;Park, Dea-Woo
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2011.10a
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    • pp.269-272
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    • 2011
  • The lawsuit is being made on the smart phone. And recent is getting a lot of evidence for the smart phone data in a court of law. Thus, the evidence of illegal use smartphone for the extraction of data and evidence collection, forensic procedure is a need for research. In this paper, evidence of phone forensic procedure for the extraction of the data suggests. And, by collecting forensic evidence from smartphones ensure the integrity of digital evidence and how to solve the case investigated. With this study, smartphone forensic will be able to contribute to the development.

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Generation of Forensic Evidence Data from Script (Script에서 Forensic 증거자료 확보 방안)

  • Kim, Seul-gi;Park, Dea-woo
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2017.10a
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    • pp.353-355
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    • 2017
  • Recently developed digital devices are being used in cyberspace. Digital device users are engaged in activities such as financial settlement and e-commerce using cyber-connected terminals. With the activation of cyber trading, cyber crimes against users are increasing. Forensic evidence should be obtained from investigations of cybercrime. However, there is a lot of information to analyze digital forensic evidence. In many of these digital information, Scripts are an effective way to secure evidence for cybercrime. In this paper, we study how to secure forensic evidence using scripts. Extract evidence from EnCase and study how to obtain evidence using scripts. This study will be used as the basic data for cyber security for the safe life of the people.

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Digital Forensics Investigation Approaches in Mitigating Cybercrimes: A Review

  • Abdullahi Aminu, Kazaure;Aman Jantan;Mohd Najwadi Yusoff
    • Journal of Information Science Theory and Practice
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    • v.11 no.4
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    • pp.14-39
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    • 2023
  • Cybercrime is a significant threat to Internet users, involving crimes committed using computers or computer networks. The landscape of cyberspace presents a complex terrain, making the task of tracing the origins of sensitive data a formidable and often elusive endeavor. However, tracing the source of sensitive data in online cyberspace is critically challenging, and detecting cyber-criminals on the other hand remains a time-consuming process, especially in social networks. Cyber-criminals target individuals for financial gain or to cause harm to their assets, resulting in the loss or theft of millions of user data over the past few decades. Forensic professionals play a vital role in conducting successful investigations and acquiring legally acceptable evidence admissible in court proceedings using modern techniques. This study aims to provide an overview of forensic investigation methods for extracting digital evidence from computer systems and mobile devices to combat persistent cybercrime. It also discusses current cybercrime issues and mitigation procedures.