• Journal of Oral Medicine and Pain
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• 제31권4호
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• pp.317-326
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• 2006

개인식별에 있어서 법치의학은 매우 빠른 속도로 발전해 왔으며 증례도 나날이 증가하고 있다. 그러나 모든 개인식별은 한 가지의 방법으로 할 수 없고 각각의 증례에서 가장 확실한 개인식별을 할 수 있는 필요한 방법들에 대한 장점과 단점을 확인하는 것이 필요하다. 본 연구에서는 4 증례를 확인하였다. 2 증례는 연령추정적 개인식별이었으며 2 증례에서는 동일인임을 확인하였다.

• Journal of Oral Medicine and Pain
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• 제35권2호
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• pp.111-117
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• 2010

법치의학적 개인식별 능력에 대한 중요성이 매우 빠르게 증가하고 있다. 그러나 개인식별은 한 가지의 방법으로만 수행되는 것은 아니다. 그러므로 각 방법의 장, 단점을 잘 파악하고 경제성 등을 고려하여 적용하여야 할 것이다. 2008년, 2009년 부산, 경남 지역에서 약 20구의 시체에 대하여 개인식별을 시행하였는데 그 중 몇 례를 실었다. 마지막으로 법치의학의 개인식별에서의 역할에 대하여 고찰해보고자 한다.

• Journal of Oral Medicine and Pain
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• 제33권2호
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• pp.177-186
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• 2008

법치의학은 많은 방법으로 정의 내릴 수 있다. 더 정밀한 정의 중 하나는 정말 법치의학은 치과전문의와 법 전문가 사이에 일부분이 겹치게 표현되는 것이다. 가장 중요한 역할은 역시 개인식별을 통한 신원 확인일 것이다. 치아의 해부학적 특징과 수복물의 독창성으로 인해 비교대상에 대한 개인식별능력이 탁월한 것이다. 우리는 2007년 한 해 동안 부산지역의 신원불상으로 밝혀진 7구의 시체에 대해 법치의학적 개인식별을 시행하여 5구의 시체에 대해 신원을 확인할 수 있었다.

• The Korean Journal of Legal Medicine
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• 제41권2호
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• pp.41-45
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• 2017

Fetal DNA (fDNA) detection in maternal serum is a challenge due to low copy number and the smaller size of fDNA fragments compared to DNA fragments derived from the mother. Massively parallel sequencing (MPS) is a useful technique for fetal genetic analysis that is able to detect and quantify small amounts of DNA. In this study, seven clinical samples of maternal serum potentially containing fDNA were analyzed with a commercial single nucleotide polymorphism (SNP) panel, the HID-Ion $AmpliSeq^{TM}$ Identity Panel, and the results were compared to those from previous studies. Reference profiles for mothers and fetuses were not available, but multiple Y chromosomal SNPs were detected in two samples, indicating that fDNA was present in the serum and thereby validating observations of autosomal SNPs. This suggests that SNP-based MPS can be valuable for fDNA detection, thereby offering an insight into fetal genetic status. This technology could also be used to detect small amounts of DNA in mixed DNA samples for forensic applications.

• 대한수사과학회지
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• 제2권2호
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• pp.50-66
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• 2007

사건 사고현장에서 가장 먼저 접근하는 초동조치 활동은 원인을 규명하는데 결정적인 역할을 한다. 법의학적 지식이 부족한 자가 최초현장에 접근하여 결정적인 사건해결 증거를 멸실케 한다면 억울한 죽음은 물론이고 무고한 혐의자를 발생시키며 유사범죄의 발생을 막을 수 없을 것이다. 본 연구는 119구급대 응급구조사에 대한 법의학교육의 필요성을 제시하고자 전국 대학(교) 응급구조학과 및 2급 양성기관의 법의학교육 실태분석을 위한 조사를 시행하였으며, 119구급대원 중 1 2급 응급구조사 307명(1급 43%, 2급 57%)을 대상으로 설문조사를 실시하였다. 그 결과 업무 중 범죄와 관련된 경험 94.8%, 죽음(변사체)의 경험이 91.9%였고, 이러한 현장에 92.8%가 경찰보다 먼저 현장에 도착하였다. 응답자의 72%는 법의학지식이 부족하여 업무 중에 어려움을 겪은 경험이 있었고, 수사경찰관과 문제, 법적증언, 유가족과의 문제 등을 경험하였다. 90.9%는 응급구조사의 업무수행에 법의학관련 지식이 도움이 된다고 긍정적으로 밝혔으며, 법의학교수에 의해 정기적인 교육을 희망하고 있었다. 응급구조사들의 법의학적 검시관련 지식을 알아보기 위해 국가 및 지방자치단체가 억울한 죽음이 없도록 감시하고, 정책적 차원에서 법의학적인 검시가 필요한 것에 대한 질문에 1급 2급 응급구조사 두 군 모두 20개 항목 중 각각 8.86개와 7.99개만 인식하고 있어, 법의학교육이 절실히 필요한 것으로 나타났다. 따라서 법의학은 응급구조사에게 단순히 범죄와 관련된 상황에서만 필요한 것이 아니라 각종 대량재해 교통사고 보험관련 사고 산업재해 등의 죽음과 관련된 법률적 문제를 해결하는 데에도 연관되어 있다. 신고체계가 다원화 되어 있는 우리나라 현실에서 119구급대의 응급구조사들은 의학적 판단에 기초한 신속하고 정확한 응급처치와 더불어 현장을 보존하고 결정적 사건해결의 물적 증거 보존을 위한 법의학적 지식을 가지기 위해서는 체계적인 법의학교육이 시행되어져야 할 것이다.

• The Korean Journal of Legal Medicine
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• 제42권4호
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• pp.141-145
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• 2018

There have been many radiographic studies on age estimation that evaluate reduction in size of dental pulp cavity with secondary dentin formation. The Paewinsky method reported high accuracy in estimating ages by measuring the width of the pulp cavity in panoramic radiographs. The aim of this study was to evaluate the application of the Paewinsky method to digital periapical radiographs. This study was conducted on 103 cases that reported to the Section of Human Identification of the National Forensic Service. The age was calculated by applying the Paewinsky method that measures the root and pulp canal width at three points in a tooth. The estimation results were compared with those calculated by the Johanson method. When the Paewinsky models were applied to digital periapical radiographs, the errors were significantly greater as compared to the original study. The errors of the maxillary second premolar and mandibular lateral incisor were greater than those of the maxillary central incisor, lateral incisor, mandibular canine, and first premolar. Furthermore, errors of the age estimation models in level C were greater than those in levels A and B. This study could be a reference for the application of the Paewinsky method to digital periapical radiographs.

• Journal of Oral Medicine and Pain
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• 제24권4호
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• pp.479-487
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• 1999

Forensic odontology and DNA typing in individual identification have been progressing rapidly and its significances has been increasing. But not all individual identification can be done by one single method solely. Therefore, understanding the advantage and disadvantage of each method is necessary to achieve forensic individual identification properly in each case for it to be applied promptly, economically and precisely.

• Journal of Korean Medical Science
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• 제33권52호
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• pp.337.1-337.14
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• 2018

Background: Mitochondrial heteroplasmy, the co-existence of different mitochondrial polymorphisms within an individual, has various forensic and clinical implications. But there is still no guideline on the application of massively parallel sequencing (MPS) in heteroplasmy detection. We present here some critical issues that should be considered in heteroplasmy studies using MPS. Methods: Among five samples with known innate heteroplasmies, two pairs of mixture were generated for artificial heteroplasmies with target minor allele frequencies (MAFs) ranging from 50% to 1%. Each sample was amplified by two-amplicon method and sequenced by Ion Torrent system. The outcomes of two different analysis tools, Torrent Suite Variant Caller (TVC) and mtDNA-Server (mDS), were compared. Results: All the innate heteroplasmies were detected correctly by both analysis tools. Average MAFs of artificial heteroplasmies correlated well to the target values. The detection rates were almost 90% for high-level heteroplasmies, but decreased for low-level heteroplasmies. TVC generally showed lower detection rates than mDS, which seems to be due to their own computation algorithms which drop out some reference-dominant heteroplasmies. Meanwhile, mDS reported several unintended low-level heteroplasmies which were suggested as nuclear mitochondrial DNA sequences. The average coverage depth of each sample placed on the same chip showed considerable variation. The increase of coverage depth had no effect on the detection rates. Conclusion: In addition to the general accuracy of the MPS application on detecting heteroplasmy, our study indicates that the understanding of the nature of mitochondrial DNA and analysis algorithm would be crucial for appropriate interpretation of MPS results.

• Journal of Oral Medicine and Pain
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• 제25권4호
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• pp.395-402
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• 2000

The D165539 locus was investigated to collect population genetic data in the Korean population. The selected subject was unrelated 293 Korean people. DNA was extracted from the samples and PCR was performed with fluorescent primer. The amplified fragment was analysed by automated DNA sequencer and it's application software. Among the Korean population, 7 allele and 18 geneotype were observed and allele No. 9 is mostly frequent(0.2679) and then allele No. 11(0.2679), allele No. 9(0.2645). The observed heterozygosity and the expected heterozygosity is 0.7466, 0.7829 each. The polymorphism information content(PIC) is 0.7466. The power of discrimination(PD) and the mean exclusion chance(MEC) are calculated to be 0.9190 and 0.5775.

• The Korean Journal of Legal Medicine
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• 제42권4호
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• pp.146-152
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• 2018

We investigated the neural correlates of accurate eyewitness memory retrieval using functional near-infrared spectroscopy. We analyzed oxygenated hemoglobin ($HbO_2$) concentration in the prefrontal cortex during eyewitness memory retrieval task and examined regional $HbO_2$ differences between observed objects (target) and unobserved objects (lure). We found that target objects elicited increased activation in the bilateral ventrolateral prefrontal cortex, which is known for monitoring retrieval processing via bottom-up attentional processing. Our results suggest bottom-up attentional mechanisms could be different during accurate eyewitness memory retrieval. These findings indicate that investigating retrieval mechanisms using functional near-infrared spectroscopy might be useful for establishing an accurate eyewitness recognition model.