• Asian-Australasian Journal of Animal Sciences
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• v.12 no.1
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• pp.129-138
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• 1999

If rumen bacteria can be manipulated to utilize nutrients (i.e., ammonia and plant cell wall carbohydrates) more completely and efficiently, the need for protein supplementation can be reduced or eliminated and the digestion of fiber in forage or agricultural residue-based diets could be enhanced. However, these approaches require a complete and accurate description of the rumen community, as well as methods for the rapid and accurate detection of microbial density, diversity, phylogeny, and gene expression. Molecular ecology techniques based on small subunit (SSU) rRNA sequences, nucleic acid probes and the polymerase chain reaction (PCR) can potentially provide a complete description of the microbial ecology of the rumen of ruminant animals. The development of these molecular tools will result in greater insights into community structure and activity of gut microbial ecosystems in relation to functional interactions between different bacteria, spatial and temporal relationships between different microorganisms and between microorganisms and reed panicles. Molecular approaches based on SSU rRNA serve to evaluate the presence of specific sequences in the community and provide a link between knowledge obtained from pure cultures and the microbial populations they represent in the rumen. The successful development and application of these methods promises to provide opportunities to link distribution and identity of gastrointestinal microbes in their natural environment with their genetic potential and in situ activities. The use of approaches for assessing pupulation dynamics as well as for assessing community functionality will result in an increased understanding and a complete description of the gastrointestinal communities of production animals fed under different dietary regimes, and lead to new strategies for improving animal growth.

• Journal of Animal Environmental Science
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• v.10 no.3
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• pp.175-182
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• 2004

A study was conducted to develop a management policy of animal waste in Northern Chungbuk province through investigating the status of animal waste management. In management of swine manure, rotary composting including a simple piling method has a portion of $64\%$ using as a liquefied fertilizer $18\%$, purification $9\%$ and ocean dumping $9\%$ respectively. Most of them use the animal waste as a fertilizer to the agricultural land. The exercise ground of dairy farms was bedded with $70\%$ of sawdust and $30\%$ of conventional soil ground besides the stall. They had a storage tank for the waste water from milking parlor. Korean beef cattle farms mostly run sawdust stall, which changed new one in a term of 6$\~$12 months. In case of layer, manure management was conducted in the order of simple piling method, rotary composting and ventilation into the piling mostly. Comparing the amount of excreted animal manure with that of possible spreading, the latter can be spread more than 2.9 times than the former in Northern Chungbuk province. The Northern Chungbuk province is an appropriate place to carry out an environmentally friendly animal production. An utilization model as a liquefied fertilizer should be developed for paddy field, upland field, orchard, and forage field to in-crease the value of animal waste effectively.

• Korean Journal of Agricultural Science
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• v.4 no.2
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• pp.178-190
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• 1977

The experimental forest of Cbungnam National University is located in Naryung-Ri, Eunsan-Myen, Buyeo-Gun, Chungcheongnamdo. The climax vegetations of Quercus species grown in centrual temperate zone are supposed to be destroyed before pure pine stands as a semi-climax vegetation are established on the southern aspect and along the ridges. Variability of vegetations is rather high and dominant species are such as Pinus densiflora, Quercus acutissima, Quercus variabilis, Quercus aliena, Quercus serrata, Lindera erythrocarpa, Platycarya strobilacea, and so on. There tare 203 woody plants and 128 herbal plants. According to the use of vegetations they can be classified as follows; 60 familia and 154 species for edible source, 63 familia and 143 species for medicinal, 51 familia and 140 species for forage, 32 familia and 78 species for ornamental, and 12 familia and 27 species for timber source.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.2
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• pp.203-206
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• 2004

Fodder supply condition was studied at 41 nomadic families in Gobi, Southern Semi-Desert area in Mongolia, from Dec. 1994 to Apr. 1995 to determine problems in cold, feed deficient season for establishment of sustainable livestock production system. The conditions of two family groups: those located in sparse vegetation (FG1), and those in comparably dense (FG2), were also compared. Commercial concentrate feed (concentrate), hay and Zoodoi were prepared for supplementation. Zoodoi was hand-made feed made mainly of Allium mongolicum and Allium polyrrhizum. Allium mongolicum tended to be used at FG1 frequently, and Allium polyrrhizum at FG2 depend on differences of micro vegetation. 44%, 90% and 39% of families prepared 165.6 kg of concentrate, 301.6 kg of hay and 6.8 kg of Zoodoi per sheep and goat (small livestock) on the average, respectively. The ratio of families that used concentrate at FG1 was smaller than those at FG2, though there were no significant differences on the amount. More hay was fed at FG1 than at FG2, and Zoodoi tended to be fed more in the FG1 group. Recipients were mostly restricted to young, female and sick small livestock that use the feeds effectively. More families gave concentrate and hay to the young than to the females and sick. They also gave more Zoodoi to young and sick animals than to females in this area. In the FG1 group, no differences were found between recipients on the concentrate supply. More families supplied hay to young animals than to sick ones, and Zoodoi was fed more to sick animals than to young and females. On the other hand, those in the FG2 found, more families fed fodders to young than to female and sick regardless of the kinds of feeds. The amount of fodder supplementation in the studied area was restricted, but accurate techniques of nomads to adapt the situation were clarified.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.106-106
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• 2017

The soybean [Glycine max (L.) Merr.] is one of the most important crop resources worldwide as food and forage. It is also important and valuable that to hold crop resources to have high genetic diversities. Recently, a core collection has been constructed in many plants to preserve the genetic resources of various plants. A core collection is small population to represent the genetic diversity of the total collection, and is of strategic importance as they allow the use of a small part of a germplasm collection that is representative of the total collection. Here, we developed the core collection consisting of 816 accessions by using approximately 180,000 (180K) single nucleotide polymorphisms (SNPs) developed in previous study. In addition, we performed genetic diversity and population structure analysis to construct the core collection from entire 4,392 collections. there were excluded sample call rates less than 93% and duplicated samples more than 99.9% according to genotype analysis using 180K SNPs from entire collections. Furthermore, we were also excluded natural hybrid resources which Glycine max and Glycine soja are mixed in half through population structure analysis. As a result, we are constructed the core collection of genetic diversity that reflects 99% of the entire collections, including 430 cultivated soybeans (Glycine max) and 386 wild soybeans (Glycine soja). The core collection developed in this study should be to provide useful materials for both soybean breeding programs and genome-wide association studies.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.4
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• pp.485-490
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• 2001

Farmers in the centre of Vietnam have a tradition of dosing young cattle with groundnut oil before fattening them on a diet of rice straw and road-side grass. These farmers claim the cattle grow faster. It was hypothesized that the effect of the oil could be to eliminate the protozoa from the rumen. This is known to increase the net microbial growth efficiency in the rumen and increase the protein supply to the animal. To test this hypothesis, two experiments were undertaken; one on-station with four cattle fitted with rumen cannulae and the second a growth trial with 25 young cattle in smallholder farms. When the cannulated animals were drenched with groundnut oil, the protozoa were eliminated from the rumen and animals could be kept free of protozoa by isolation. The ammonia concentration in the rumen fluid was decreased when the protozoa were eliminated and there was an indication of improved rumen dry matter degradability of the forage components of the diet. In the practical condition on the smallholder farms, the growth rates of cattle drenched with groundnut oil were increased considerably (65%) compared with untreated control animals. The laboratory results when taken together with the on-farm results indicate that these resource-poor farmers had been able to defaunate their cattle and to maintain the fauna-free state by isolation of their animals from extraneous stock. This traditional practice in Central Vietnam, whereby one family keeps only one or two animals that are hand fed and tethered, has quite a large potential for all of those countries where animals are fed agro-industrial by-products, as it is highly economic. The use of 1 litre of oil compared with 1 kg of rice polishing per day (300 kg over 300 days), would be highly profitable in all countries of South-East Asia.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.7
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• pp.980-989
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• 2005

The influence of treated, extruded, partially expelled soybean meals as undegradable protein and bypass fat sources on lactation performance and ruminal fermentation of dairy cows was studied. Experiment 1: nine cows were used in a replicated 3${\times}$3 Latin square design with each period being 3 wk in duration. Cows were fed 440 g/kg forage and 560 g/kg grain diet with one of three extruded soybean meals fed at 110 g/kg of the diet. The 3 soybean meals were 1) twice-extruded soybean meal (ESM; as a control); 2) lignosulfonate-treated, twice-extruded soybean meal (LSM); and 3) calcium oxide plus lignosulfonate-treated, twice extruded soybean meal (CLSM). Experiment 2: 3 ruminally cannulated cows were used in a 3${\times}$3 Latin square to study the treatment influence on ruminal fermentation characteristics. Feeding treated soybean meal to cows in LSM and CLSM treatments did not improve feed intake, milk yield, or milk composition except that cows fed the LSM and CLSM treatments produced less milk protein compared with the ESM treatment. The proportion of $C_{18:2}$ was greater in milk fat of cows fed CLSM compared with that of cows fed the ESM or LSM treatments. Ruminal pH, ammonia, and total volatile fatty acids were not affected by treatment. An increased proportion of $C_{18:2}$ in milk fat suggests that there is a potential use of calcium salts of fatty acids in protecting the lipid portion of extruded soybean meal and further research is needed to explore this potential with full-fat extruded soybeans not with extruded and partially oil expelled soybeans.

• Korean Journal of Organic Agriculture
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• v.17 no.3
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• pp.307-326
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• 2009

The present study suggests a concept of resources circulation type agriculture and a concept of a livestock zone related to it and evaluated economical efficiency about a possibility of the resources circulation type livestock zone based on the concept. As a result, when the resources circulation type livestock zone is operated in an area of 1,300ha, it was evaluated that the profit of 11,244 hundred thousand won per year is generated, compared to the conventional agriculture. When light and heat expenses and roughage of Hanwoo production, production of TMR forage as well as income of the energy and composting business of livestock manure at the recycling center were considered in addition to that, it was judged that the present zone development bushiness has enough business feasibility. However, it is necessary that the support regulations, etc. is constructed so that all produced compost byproducts are used at farmhouses and the system related to facility investment assurance and assistance of operating expenses is maintained. It is thought that the complement of a system is also necessary so that energy or power generation expenses can be operated in the same conditions as other new & renewable energy, and the nation's purchase of carbon credits(CDM) is investigated and systemized in the early period, and construction of a revitalization plan of the business through budget securing of the Ministry of Agriculture and Forestry related to the trade of CDM and carbon emission rights is also a business that should be urgently developed. In the future, the change of an outside environment will further promote use of the energy. Also, as people, who don't know agriculture. farm village, increase day by day because of population concentration in a city, an opportunity that can new added value through experience tourism or educational programs utilizing them is increasing. Accordingly, if programs utilizing social characteristics, such as utilization as a space of tourism. leisure. experience, system construction of bio-energy and system construction that Micro Grid or Smart Grid as a dispersed storage and generation system is stabilized, are constructed, it can be said that revitalization or success conditions of the zone is further achieved.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.2
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• pp.196-200
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• 1993

A full understanding of the interdependence of leaf nitrate (($No_3$￣) metabolism and symbiotic nitrogen($N_2$) fixation in legume crops is needed to help maximize the use of both N sources as well as to improve forage quality through the inhibition of leaf nitrate accumulation. The present work examines the effects of added nitrate, the level of which are 0,2,4,8 and 12mM, on the nodule formation and leaf nitrate utilization and on the possibility of inducing nitrate-toxicity to livestocks in two alfalfa varieties, ' Vernal ' of grazing type and ' Victoria ' of hay type. Higher level of exogeneous nitrate resulted in the increased above-ground dry weight. Nodulation was inhibited severely when more than 8mM NO$_3$￣ was supplied to alfalfa plants, and leaf nitrate reductase reached a maximunm at 4mM nitrate supply. The $V_{max}$of nitrate reductase in leaves of Vernal was similar to that of Victoria, whereas the $K_m$ of Vernal was higher than that of Victoria. High accumulation of leaf nitrate, $4{\times}10^{-5}$ g/g leaf fresh weight, was shown at 12mM nitrate supply, which was thought to be not enough to induce nitrate-toxicity to livestocks.icity to livestocks.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.1
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• pp.140-146
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• 2000

Even though coix (Coix lachryma-jobi L. var. ma-yuen) has many physiological effects, since it has been known to cause sterility, farmers avoid using coix bran as a forage for their livestock. Therefore, as the consumption of coix increases, coix bran, which is a by product of pounding process, becomes a serious issue of environmental problem. Present study examined the physiological effects of coix bran in normal and diabetic rats for its possible use as a functional material. The effects of coix bran supplementation on plasma and hepatic lipid profile were evaluated in rats fed one of the following diet for 12 weeks : chow diet ; chow-bran diet (chow diet＋25% coix bran), high fat diet and high fat-bran diet (high fat diet＋25% coix bran). Additionally, glucose challenge and lipid metabolism in streptozotocin-diabetic rats were also examined. In normal rats, consumption of coix bran remarkably reduced body weight gain in chow or high fat diet fed rats. Additionally, consumption of coix branreduced blood TG, TC and atherogenic index (26%, 24% and 72%, respectively) in chow diet fed rats. Liver TG and cholesterol concentrations were reduced (43% and 49%, respectively) in high fat fed rats by coix bran supplementation. In diabetic rats, fasting blood glucose level was reduced about 25% by coix bran consumption. Also, glucose challenge pattern was improved and resembled normal pattern : it reaches to peak 15~30 minutes after glucose administration and get back to fasting blood glucose level after 90 minutes. Plasma concentrations of TG were elevated in diabetic rats and were reduced to normal level by coix bran supplementation. Liver TG and cholesterol concentrations were also elevated in diabetic rats and reduced to normal level by consumption of coix bran. These results suggest that coix bran may have beneficial effects on blood lipid and glucose level in normal and diabetic rats.