• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.4
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• pp.303-310
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• 1998

We studied expression patterns of thermotolerance gene (BcHSP17.6) in cabbages which was isolated from Chinese cabbage and we will attempt transformation of forage crops with the gene in order to increase thermotolerance of forage crops. Antiserum against a BcHSP17.6 protein was reacted with its antigen. With this antiserum, the accumulation of the 15- to 18-kD LMW HSPs under various heat shock (HS) conditions was quantified. The LMW HSPs began to be detectable at $35^{\circ}C$, and after 4 hours at $40^{\circ}C$ they were accumulated to a maximum level of 1.56 micrograms per 100 micrograms of total proteins in cabbage leaves and remained almost unchanged up to 24 hours after HS. Accumulation of the HSPs was reduced at temperatures higher than $40^{\circ}C$. We conclude that accumulation of these LMW HSPs are necessary for Chinese cabbages to survive at an otherwise lethal temperature.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.4
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• pp.241-248
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• 2007

Cytokinins are essential plant hormones that play crucial roles in various aspects of plant growth and development. By using mRNA differential display, we isolated a cytokinine-inducible cDNA encoding pathogenesis-related (PR) 4 from Arabidopsis amp1 mutant. The full-length PR4 cDNA, designated AtPR4, contains an open reading frame of 212 amino acids with calculated molecular mass of 22,900 Da and isoelectric point (pI) of 7.89. Genomic DNA blotting showed that the Arabidopsis genome has one copy of AtPR4. AtPR4 mRNA was induced by cytokinin and NaCl, but decreased by SA or JA treatment. PR proteins are induced in response to pathogen attack. Thus the AtPR4 gene isolated in this study may be a useful candidate for genetic engineering of forage crops for increased tolerance against pathogen.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.3
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• pp.164-168
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• 1995

A field experiment was carried out to determine the effects of nitrogen (N) fertilization levels after first harvest (0, 30, 60, 90 and 120 kglha) during spring season on the gms growth, dry matter (DM) yield, nutritive value, and efficiency of DM production per N kg applied in orchardgrass meadow. Additional N was applicated at early spring (70 kg), after second harvest (50 kg), and after fourth harvest (30 kg/ha) in all treatments, 1990. Growth and DM yield of orchardgrass at second harvest were significantly increased with increasing level of N after first harvest (P<0.05), but no yield differences were observed between application of N 60, N 90, and N 120 kg. The efficiency of DM increment per N kg applied was rapidly decreased with increasing level of N; that was 14.8, 12.0, 10.7 and 4.7 DM kg/N kgha in the plot of N 30, N 60, N 90 and N 120 kg, respectively. The content and yield of crude protein of orchardgrass at second harvest were significantly increased with increasing of N (P<0.05), ranged h r n 11.0% (N 0 kg) to 16.8% (N 120 kg), and from 195.0 kg (N 0 kg) to 508.2 kglha (N 120 kg), respectively. However, the content of neutral detergent fiber was not affected by N level after first harvest. From the above results, it is suggested that application of N fertilizer after first harvest was very effective for regrowth and nutritive value of orchardgrass during spring season, and the proper amount of N after first harvest was 60-90 kglha in this experiment.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.2
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• pp.118-123
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• 1995

This experiment was canied out to determine the effects of nitrogen (N) fertilization level (0, 70, 140, 280 and 420 k a a ) on the grass growth, dry matter (DM) production, numtive value, and botanical composition in reed canarygrass (Phalaris ancndinacea L.) pasture. \ulcornerhe cultivar used in this study was Palaton, and the grass was harvested at soiling-hay stage (3 times per year) in 1992 and 1993. As the N level was increased, the plant height, DM produdion, and crude protein (CP) content were significantly increased (P<0.05). When the N was applied at the levels of 0, 70, 140, 280, 420 kg, DM yields were 4,540, 5,316, 6,060, 7,923 and 9,960 kgha, and CP contents were 17.6, 17.4, 17.4, 18.4 and 19.8%, respectively. However, as the N level was increased, DM percentage, total digestible nutrients, and relative feed value were decreased, while the contents of neutral detergent fiber and acid detergent fiber, and bareland were continuously increased, particularly in the plot of N 420 kg fdzation. From the above results, it is suggested that reed canarygrass was one of the sensitive pasture grasses to N fertilizer, and the proper amount of N application was 280 kg/ha/yr for desirable production, nutritive value, and botanical composition in r e d conarygrass pasmre.

• Journal of The Korean Society of Grassland and Forage Science
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• v.41 no.3
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• pp.198-204
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• 2021

Intercropping cereals with legumes is known to improve forage production and crude protein yield. Sorghum × sudangrass hybrids (SSH) have excellent dry matter content and high cultivation temperatures. In this study, we investigated the growth characteristics, forage productivity, and feed value of intercropping SSH with different legumes in rice paddy fields. We used five treatments in this study SSH monocropping and four intercropping treatments of SSH with, lablab, cowpea, sesbania, and two cultivars of soybean (Chookdu 1 and 2). SSH plant height was not significantly different between the monocropping and intercropping treatments. However, the plant heights of lablab, cowpea, and sesbania were significantly higher than those of the two soybean cultivars. The total dry matter yield (kg/ha) was significantly higher in SSH monocropping than in intercropping; among the intercropping treatments, the one with SSH and Chookdu 2 yielded the highest total dry matter yield. The SSH feed value was significantly different between the monocropping and intercropping treatments, although there were no differences between the intercropping treatments. Among the intercropped legumes, lablab showed the highest neutral detergent fiber and acid detergent fiber contents, and cowpea had the highest crude protein content. These results reveal that intercropping SSH with legumes in paddy fields could be a promising cultivation technique to maintain stable forage productivity.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.4
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• pp.293-300
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• 2006

This study was carried out to determine the effect of chemical fertilizer and two fermented types of pig slurry on the dry matter(DM) yield of three crops and chemical properties. The experiment design was a split plot with three replications. Main plots consist of three crops : $sorghum{\times}sudangrass$ hybrid('SS405'), sudangrass('Jumbo'), corn('DK501'). Sub plots consist of three treatments : chemical fertilizer (CF N-200, P-150, K-150 kg/ha), aerobic fermented pig slurry (APS 200kg N/ha), and aerobic fermented pig slurry treated with probiotics (APS+P 200 kg N/ha). Plant heights with three crops per sudangrass (380.3cm) was the longest (p<0.01). Dry matter yield of aerobic fermented pig slurry treated with probiotics was the highest the other treatments (p<0.01). Crude protein (CP) content were highest in $sorghum{\times}sudangrass$ hybrid than in the other crops. Cupper content(%) were highest in aerobic fermented pig slurry treated with probiotics than in the other treatments.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.4
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• pp.309-315
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• 2015

In order to identify the causes of various problems related to forage crop growth, such as winter survival, coldness, rainfall, drought etc., and to provide basic data for the stable production and supply of forage year round, we performed a growth survey after the wintering of winter forage crops grown from mid-Sep. 2014 to late-Feb. 2015. The growth of winter forage crops after wintering in the country was generally bad. As shown in the regional distribution in the country, regions with 80% or higher winter survival rates comprised 66%, regions with 79 to 50% winter survival comprised 24.9% and regions with less than 50% winter survival comprised 9.1%. In conclusion, the average winter survival rate was 79% in the country. Winter survival rate and coverage rate after the wintering of winter forage crops under installed drain channels in paddy fields were good at 83% and 80%, respectively. However, the rates without installed drain channels were bad at 67% and 66%, respectively. It was predicted that the crop production of winter forage crops was reduced by 10-15% in Gangwon, Chungbuk, Chungnam, Gyeongnam and Jeonnam regions, reduced by 30% in Gyeonggi, Gyeongbuk and Jeonbuk regions and reduced overall by approximately 19% nationwide.

• Korean Journal of Agricultural and Forest Meteorology
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• v.25 no.2
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• pp.61-70
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• 2023

In order to select high-quality winter cereal crops with high yield and to increase self-sufficiency rate of forage, their growth, yield, and feeding value of several cereal crops cultivated in winter were investigated in the paddy field of the southern region. Four wheat cultivars and green barley headed in early and mid-April, while oat and Italian ryegrass headed in early May. Fresh forage yields of wheats, green barley, and oat were significantly higher than that of Italian ryegrass, and dry forage yields of wheats and green barley were significantly higher than those of not only Italian ryegrass but also oat. In particular, the yield of a wheat cultivar 'Cheongwoo' was the highest. Mineral contents of wheat forages, even though low, were in the range 27.8~33.7mg·g^-1 DW suitable for feeding cattle and young female cows. Crude protein content of a wheat cultivar 'Cheongwoo' was high up to 7.6%, similarly to 7.0% requiring for feeding cattle. Feeding values such as total digestible nutrients (TDN) and relative feed value (RFV) of wheats and green barley were superior to those of oat and Italian ryegrass. In addition, dry matter rates of 4 wheat cultivars and green barley were in the range 30~40%, indicating that wheat cultivars and green barley could be used for various feeding purposes such as green or dried forage, and silage. Based on these results, wheat cultivars including 'Cheongwoo' and green barley could be encouraged to be cultivated in paddy fields, as high-quality winter forage crops with high yield.

• Journal of The Korean Society of Grassland and Forage Science
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• v.40 no.3
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• pp.190-195
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• 2020

Cadmium (Cd) toxicity is a serious limitation for agricultural production. In this study, we explored tolerance mechanism associated with Cd toxicity tolerance in alfalfa plants. We used three distinct alfalfa cultivars M. sativa cv. Vernal, M. sativa cv. Zhung Mu, and M. sativa cv. Xing Jiang Daye in this study. Cd showed declined chlorophyll score in Xing Jiang Daye compared with Zhung Mu and Vernal. No significant change observed among the cultivars for root and shoot length. Atomic absorption spectroscopy analysis demonstrated a significant accumulation of Cd, Fe, S and PC in distinct alfalfa cultivars. However, Zhung Mu and Xing Jiang Daye declined Cd accumulation in root, where Fe, S and PC incremented only in Zhung Mu. It suggests that excess Cd in Zhung Mu possibly inhibited in root by the increased accumulation of Fe, S and PC. This was further confirmed by the response of Fe (MsIRT1) and S transporters (MsSULTR1;2 and MsSULTR1;3), and MsPCS1 genes associated with Fe, S and PC availability and translocation in roots and shoots. It suggests that specially the transcript signal inducing the responses to adjust Cd especially in Zhung Mu. This finding provides the essential background for further molecular breeding program for forage crops.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.220-220
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• 2017

Soybean (Glycine max (L.) Merill) is a very outstanding material crop with high protein and oil contents. We conducted this study to evaluate forage value and usability of soybean varieties as livestock forage. Three soybeans cultivars, OT93-26, Geomjeongsaeol, and Pungwon, were evaluated for forage use in this study, and Kwangpyeongok and Yeongwoo were used as check forage corn and rice, respectively. The whole soybean plants were harvested at the R5 (beginning seed development)- and R6 (full seed)-reproductive stages for analyzing forage usability and quality. Days to harvesting of the R5 stage-OT93-26 was the shortest among the tested crops while that of Yeongwoo was 122 days. The fresh and dry matter yields of all 3 soybeans were greater at R6 stage than at R5. Crude protein of the soybean cultivars harvested had a higher compared to the rice and corn regardless of the harvesting stage. Contents of crude fiber, neutral detergent fiber and acid detergent fiber of Yeongwoo had the lowest whereas Pungwon harvested at R5 were the highest. Among the soybeans, digestible dry matter, dry matter intake, and relative feed value of R6-harvested Geomjeongsaeol and Pungwon were high more compared to those at the R5-harvested, but in case of OT93-26 those at R6 stage were inversely measured rather than those at R5 stage. In conclusion, soybean can be used as s forage with high nutritive value for livestock. Moreover, Geomjeongsaeol can be applied to develop new forage soybean varieties with high nutritive value, and R6 stage is the optimum harvesting stage in yield and quality of the tested soybeans more than R5.