• 한국초지조사료학회지
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• 제15권1호
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• pp.43-51
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• 1995

Tlis experiment was to study the effects of application rates of mineral N and cattle slurry on the dry matter yield and botanical compositions of Orchardgrass sward grown in 4 cuttings in a year. Annual rates of mineral N of O($N_0$), 120kg(($N_1$), 240kg(($N_2$) and 360kg/ha(($N_3$) were applied as urea, and cattle slurry applied at rates of O(($N_0=N_0$), $40m^3$($S_1$), $80m^3$(($S_2$) and $120m^3$($S_3$), suppling 120kg, 240kg and 360kgl ha, respectively. The results were summarized as follows; 1. The limiting annual application rates of mineral N and cattle slurry was estimated about 120kg/ ha. 2. The growth of Orchardgrass after the 3rd cut was decreased by summer depression. Consequently, the botanical compositions of Orchardgrass showed only 7.7% and 4.0% in 3rd and 4th cut, respectively. However, the 15 and 17 species of weeds originated in 3rd and 4th cut. 3. The botanical compositions of Amaranthus mangostanus in 3rd cut and Portulacea aleracea in 4th cut were greatly increased with application rates of cattle sluny. 4. Relative efficiency of cattle sluny for the dry matter production of Orchardgrass as compared to mineral N were 93.396, 99.5% and 113.6% in 120kg, 240kg and 360kglha at rates of cattle slurry, respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제9권1호
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• pp.75-81
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• 1996

The dry matter (DM) disappearance and ruminal solubility of calcium (Ca), phosphorus (P), magnesium (Mg) and potassium (K) in eight Philippine forages were studied. The forages were: paragrass (Brachiaria mutica (Forsk.) Stapf), stargrass (Cynodon plectostachyum Pilger), napiergrass (Pennisetum purpureum Schumach), clopo (Calopogonium mucconoides Desv.), centrocema (Centrocema pubescens Benth.), gliricidia (Gliricidia sepium (Jacq.) Walp.), leucaena (Leucaena leucocephala (Lam.) de Wit.) and sesbania (Sesbania grandiflora (L.) Poir. Nylon bags with samples were incubated for 0, 3, 6, 12, 24, 48 and 72 h in rumen cannulated sheep. The 0-h bags were washed with deionized water. For the 0-h samples, 20.4, 17.2, 50.7, 52.2 and 80.1% of the DM, Ca, P, Mg and K was solubilized, respectively. At 3-h incubation period, DM disappearance was 10 percentage units higher than that of 0-h incubation whereas mineral disappearance increased by 43, 21, 30 and 13% for Ca, P, Mg and K, respectively. At 72-h incubation period, greater proportion of DM, Ca, especially in P, Mg and K was solubilized with a value of 73.8, 71.5, 85.6, 91.4 and 98.2%, respectively. The average particulate passage rate obtained in the present study was 1.9%/h where as the range of disappearance rates of various mineral elements were : 0.4 to 1.2%/h for Ca, 0.1 to 1.6%/h for P, 0.7 to 2%/h for Mg and 0.1 to 2%/h for K. The effective ruminal solubilization (ERS) of the macrominerals was calculated where particulate passage rate and disappearance rate of the various elements were included in the equation. The ERS of Ca, P, Mg and K was 50.0, 72.6, 83.9 and 94.5%, respectively. Species differences (p<0.05) on the various mineral solubilities were also observed. This study shows that ruminal solubility of macrominerals in selected Philippine forages is K > Mg > P > Ca.

• Asian-Australasian Journal of Animal Sciences
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• 제29권1호
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• pp.51-61
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• 2016

In order to provide recommendations on the most useful forage species to smallholder farmers, eleven grass and eleven legume forages grown in Abomey-Calavi in Republic of Benin were investigated for nutritive value (i.e. chemical composition and energy content) and fermentation characteristics (i.e. gas and volatile fatty acid production, organic matter degradability). The in vitro gas production technique was used, incubating the forages for 120 h under anaerobic condition with buffalo rumen fluid. Compared to legume, tropical grass forages showed lower energy (8.07 vs 10.57 MJ/kg dry matter [DM]) and crude protein level (16.10% vs 19.91% DM) and higher cell wall content (neutral detergent fiber: 63.8% vs 40.45% DM), respectively. In grass forages, the chemical composition showed a quite high crude protein content; the in vitro degradability was slightly lower than the range of tropical pasture. The woody legumes were richer in protein and energy and lower in structural carbohydrates than herbaceous plants, however, their in vitro results are influenced by the presence of complex compounds (i.e. tannins). Significant correlations were found between chemical composition and in vitro fermentation characteristics. The in vitro gas production method appears to be a suitable technique for the evaluation of the nutritive value of forages in developing countries.

• Asian-Australasian Journal of Animal Sciences
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• 제25권6호
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• pp.818-823
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• 2012

Tall fescue (Festuca arundinacea Schreb.) is an important cool season forage plant that is not well suited to extreme heat, salts, or heavy metals. To develop transgenic tall fescue plants with enhanced tolerance to abiotic stress, we introduced an alfalfa Hsp23 gene expression vector construct through Agrobacterium-mediated transformation. Integration and expression of the transgene were confirmed by polymerase chain reaction, northern blot, and western blot analyses. Under normal growth conditions, there was no significant difference in the growth of the transgenic plants and the non-transgenic controls. However, when exposed to various stresses such as salt or arsenic, transgenic plants showed a significantly lower accumulation of hydrogen peroxide and thiobarbituric acid reactive substances than control plants. The reduced accumulation of thiobarbituric acid reactive substances indicates that the transgenic plants possessed a more efficient reactive oxygen species-scavenging system. We speculate that the high levels of MsHsp23 proteins in the transgenic plants protect leaves from oxidative damage through chaperon and antioxidant activities. These results suggest that MsHsp23 confers abiotic stress tolerance in transgenic tall fescue and may be useful in developing stress tolerance in other crops.

• 한국초지조사료학회지
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• 제14권4호
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• pp.288-294
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• 1994

Birdsfoot trefoil(MO-20)과 tetraploid narrowleaf trefoil(PI302921)및 이들 종간 잡종의 유식물 확력을 건물축적량 광합성율 생장분석 등을 통하여 비교검토하였다. 일반적으로 종간 잡종의 표현형은 그 들 부모의 중간 특성을 나타내었으며, 암호흡, 광합성 및 휴식물생육은 이 들 모계 계통인 MO-20 birdsfoot trefoil과 유사하였다. 중간잡종은 birdsfoot trefoil 보다 빨리 자랐으나 통계적인 유의성은 없었다. 또한 교잡종은 narrowleaf trefoil보다 유식물 활력이 더 우수하였는데 그것은 낮은 암호흡율과 높은 상대성장율에 기인되었다. 앞으로 birdsfoot trefoil의 유식물 활력의 유전적인 개량은 새로운 분야로서 개량의 여지가 많음을 보여 주었다.

• 한국초지조사료학회지
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• 제12권3호
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• pp.153-160
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• 1992

본 시험은 고령지 두과목초의 적응성을 구명하기 위하여 표고가 다른 3개지역(600,800,1000m a.s.l)에서 주구를 파종시기(추파.추파), 세구를 혼파조명(알팔파-화본과, 레드크로바-화본과, 화이트크로바-화본과)으로 두고 분할구 배치 3반복으로 수행하였다. 1.600m에서 두과목초의 적응성을 보면 알팔파, 레드클러버 및 화이트클로버 공히 추파를 했을 때 연차간 악화없이 약 30%를 유지하여 적응성이 매우 양호하였다.2. 800m에서는 화치트클로버를 춘파하므로써 연차간에 심한 감소없이 최종 예취시 약 14.0%를 점유하고 있었으며 또한 10a당 건물수량이 가장 많았다. 3. 1000m에서도 800m와 비슷한 경향으로 화이트클로버를 춘파했을 경우 어느정도 적응이 가능하였다. 4. 표고별 두과목초의 비솔 및 수량이 표고가 증가 할수록 현저하게 감소하였으며 알팔파는 800m 이상에서는 생육이 거의 불가능하였다.

• Journal of Plant Biotechnology
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• 제5권2호
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• pp.87-93
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• 2003

Brachiaria (Poaceae) is the most important forage genus for cattle production in Brazil. The genetic breeding of this genus is limited by the incompatibility among species, differences in ploidy level and the natural cloning of plants by apomixis (Valle and Miles 1992). However, plant regeneration via tissue culture methods and genetic engineering provide an opportunity to introduce new characteristics in plants of this genus. We have developed methods for the 'genetic modification of Brachiaria brizantha cv. Marandu via biolistic transformation. A higher number of shoots was obtained with 4 mg/L 2.4-diclorophenoxyacetic acid and 0.2 mg/L benzylaminopurine in calli induction medium and 0.1 mg/L naphtaleneacetic acid and 4.0 mg/L kinetin in shoot regeneration medium. A selection curve for mannose was determined to use phospho mannose isomerase (PMI) gene of Escherichia coli as a selection marker. Calli formation was inhibited from 5 g/L mannose, even in the presence of sucrose while calli that were formed in the presence of mannose failed to develop embryos showing that PMI gene can be used for selection of transformants of this grass. Different promoters were tested to evaluate the efficiency based on the detection of the GUS gene expression (Jefferson et al. 1987). The monocot promoters, act1-D and ubi-1, resulted in higher expression levels than dicot promoters, ubi-3 and act-2, or the CaMV35S and CVMV promoters.

• 한국초지조사료학회지
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• 제10권3호
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• pp.141-146
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• 1990

Changes in dry matter yield, crude components, nutrients uptake($P_2O_5$, $K_2O$, CaO, MgO) and sulphur containing amino acid(cysteine, methionine) of orchardgrass(Dacty1is glomerata. L) and alfalfa(A4edicago sativa. L) by gypsum application(as sulphur source, 0, 2. 5, 10, 20kg SIlOa) were investigated to understand the effect of sulphur on herbage production in pasture, which was established in 1987 as means of hand broadcasting. The effect of gypsum on dry matter yield at different cutting times during growing seasons has not been found both in orchardgrass and in alfalfa, but in respect to annual total dry matter yield there were increment in herbage yield (P<0.05) of alfalfa at 5, 10, 20kg SIlOa in 1989 and the amounts of sulphur taken up in herbage slightly increased according to the rates of gypsum application. Maximum apparent recovery of sulphur was 7.55% at 2kg SIlOa in orchardgrass and was 17.8% at 5kg S/lOa in alfalfa. There were no any great differences in the content of crude components of both species and this trend was similar with the mineral contents of orchardgrass. But in alfalfa, the amounts of $K_2O$, CaO, and $P_2O_5$ taken up were increased by gypsum application and the increment in the amounts of minerals taken up in herbage at 20kg SIlOa were 14.9 of $K_2O$, 9.1 of CaO, and 2.5kgIlOa of $P_2O_5$ as compared to those of at untreated plot. Cysteine and methionine were not influenced by gypsum applicaton not only in orchardgrass but also cysteine in alfalfa, however, the content of methionine in alfalfa was slightly increased at 2, 5, lOkg SIlOa and at 20kg SIlOa was reverse.

• 한국초지조사료학회지
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• 제4권2호
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• pp.127-132
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• 1983

冠岳山의 억새 草地A와 B 및 새草地에 있어서 落葉의 生産, 堆積 및 分解를 硏究하였다. 1. 억새草地A와 B 및 새草地 落葉의 生産量은 各各 2,267.12, 943.44 및 1,228.48g/m$^{2}$였다. 2. 억새草地A와 B 및 새草地 落葉有機物의 分解常數는 各各 0.732, 0.411 및 0.877 이였다. 3. 草地落葉의 有機物이 억새草地A와 B 및 새草地에서 半減하는데 要하는 年數는 各各 0.9年 1.7年 및 0.8年이었고 95% 減少하는데는 4.1年, 7.3年 및 3.4年이었으며 99% 減少하는데는 6.8年, 12.2年 및 5.7年이었다. 4. 草地落葉의 分解速度에는 土壤含水量과 草種에 따라 顯著 差異가 있었다.

• 한국초지조사료학회지
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• 제4권2호
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• pp.152-157
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• 1983

濟州道內 6個部落 共同牧場 野草地를 對象으로 野草의 生産性과 植生比率이 家畜 增體에 미치는 效果를 究明하기 위해 1981년 5월부터 동년 11월까지 調査, 分析한 結果는 다음과 같다. 1. 乾物收量은 7月收穫보다 10月收穫에서 2培二上 높았으며 모든 牧場이 같은 趨勢였다. 2. 野草의 祖蛋白質 含量은 7월보다 10월에서 낮았으며 粗纖維는 그 반대였다. 3. 牧場別로 볼 때 1일 增體量이 높은 河源과 海安牧場이 野草의 組蛋白質 含量이 가장 높았고 增體率이 제일 낮은 吾羅牧場에서 組蛋白質 含量이 낮았다. 4. 本 野草地에 分包된 全植生中 잔디가 56.61%로서 가장 높았으며 그 다음은 고사리 16.44%, 억새 3.76% 순위였다. 科別로는 禾本科가 72.08%. 登科 1.81% 고사리과 16.49%, 기타 9.62%였다. 5. 1일 增體率이 높은 河源牧場은 禾本科와 登科의 比率이 높았고 增體率이 낮은 吾羅와 龍崗牧場은 독생이 있는 고사리 比率이 높은 것으로 나타났다.