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Effects of Homolactic Bacterial Inoculant Alone or Combined with an Anionic Surfactant on Fermentation, Aerobic Stability and In situ Ruminal Degradability of Barley Silage

  • Baah, J.;Addah, W.;Okine, E.K.;McAllister, T.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.3
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    • pp.369-378
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    • 2011
  • The effect of a homolactic inoculant containing a blend of Lactobacillus plantarum, Pediococcus acidilactici and Enterococcus faecium or, the anionic surfactant, sodium dodecyl sulphate (SDS), alone or in combination on fermentation characteristics, aerobic stability and in situ DM, OM and NDF degradability of barley silage was investigated. Barley (Hordeum vulgare, L.) was harvested (45% DM), chopped and treated with water at 24 ml/kg forage (Control), inoculant at $1.09{\times}10^5$ cfu/g forage (I), SDS at 0.125% (wt/wt) of forage (S) or with the inoculant ($1.09{\times}10^5$ cfu/g) plus SDS (0.125% wt/wt; I+S). The treated forages were ensiled in triplicate mini silos and opened for chemical and microbiological analyses on d 1, 2, 3, 7, 14, 42 and 77. Silage samples from d 77 were opened and aerobically exposed for 7 d. The in situ rumen degradability characteristics of silage DM, OM and NDF were also determined. The terminal concentration of NDF in S and I+S was lower (p<0.001) than in other treatments. Lactate concentration was higher (p<0.001) and the rate and extent of pH decline were greater (p<0.001) in I and I+S than S and Control silages. A homolactic pathway of fermentation in I and I+S was evidenced by reduced (p<0.001) water-soluble carbohydrates concentration, higher lactate (p<0.01), lower acetate (p<0.01) and lower pH values (p<0.001) than in S and Control silages. All silages remained stable over 7 d of exposure to air as indicated by lower temperatures and moulds, and by non-detectable yeast populations. The treated silages had lower DM and OM degradability than in the Control but NDF degradation characteristics of I+S were improved compared to other treatments. It is concluded that the inoculant alone improved the fermentation characteristics whereas the combination of the inoculant with SDS improved both fermentation and NDF degradability of barley silage.

Effects of Nitrogen Fertilization on Forage Production and Nutritive Nalue of Geukdong 6, Teosinte Hybrid [Zea mays L. subsp. mexicana (Schrad.) H. H. Iltis]

  • Wang, Chengyu;Lee, Sang Moo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.40 no.1
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    • pp.37-43
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    • 2020
  • This study was conducted to evaluate the effects of different nitrogen levels on the yield and nutrient quality of a newly developed domestic hybrid of Teosinte, Geukdong 6〔Zea mays L. subsp. mexicana (Schrad.) H. H. Iltis〕. The field experiment was conducted in a randomized block design with three replicates and consisted of four nitrogen (N) application rates, T1 (200 kg/ha), T2 (300 kg/ha), T3 (400 kg/ha), and T4 (500 kg/ha). No differences were found in plant length, leaf length, leaf width, leaf number, dead leaves, stem hardness, tiller number, and fresh yield (p>0.05). The T3 showed significantly greater dry matter yield at harvest (heading stage) compared to the other treatments (p<0.05). The crude protein content of T4 (10.49%) was higher than those of T1 and T2 (p<0.05). However, there was no significant difference between T4 (10.49%) and T3 (9.63%). The effects on crude fat, crude ash, neutral detergent fiber (NDF), acid detergent fiber (ADF), and crude fiber were not significant (p>0.05). The sugar content was higher in the T2 treatment than the other treatments (p<0.05). For Ca, T3 showed significantly greater content (p<0.05). However, no significant effects were found in the contents of Cu, Fe, K, Mg, Mn, Mo, and Zn (p>0.05). Na content was higher in order of T2 > T4 > T3 > T1 (p<0.05). Total mineral contents were not significantly different among the treatments (p>0.05). Given these results, we recommend the amount of nitrogen fertilization necessary for "Geukdong 6" to be around 400 kg per ha (T3), when considering, high fresh yield, dry matter yield, number of leaves and content of crude protein.

Feed Value and Fermentative Quality of Haylage of Winter Cereal Crops for Forage at Different Growing Stages (사료맥류의 생육단계별 헤일리지 사료가치 및 발효품질)

  • Song, Tae Hwa;Park, Tae Il;Han, Ouk Kyu;Kim, Kee Jong;Park, Ki Hun
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.32 no.4
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    • pp.419-428
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    • 2012
  • This study was carried out to analyze winter cereal crops for harvest time, proper drying time and haylage quality in order to investigate the possibility of the production of good quality haylage substitute for hay. As a result, in case of productivity and feed value, dry matter yield and TDN yield was increased with late harvest and crude protein and NDF, ADF was decreased (p<0.05). To make haylage for whole crop barley, oats, triticale, whole crop wheat need more than 32 hour wilting time in the milk ripe stage, yellow ripe stage need about 8~24 hour, and wilting time did not need the full ripe stage. Rye does not suitable for making haylage because of difficulty pre-wilting time. In case of fermentative quality on haylage, pH was increased with late harvest on all winter cereal crop forage. Lactic acid content was decreased with late harvest (p<0.05), and highest in the milk ripe stage. Acetic acid was also decreased with late harvest, and butyric acid was not detected. In conclusion, to harvest sooner of winter cereal crops could be an efficient way when making haylage in terms of protein content and fermentation quality. Ray can used as fresh and silage, because difficulty for pre-wilting time in Korea. Whole crop barley, oats, triticale, wheat can be used haylage when harvested yellow ripen stage and pre-wilting time for 8~24 hours.

Himalayan dock (Rumex nepalensis): the flip side of obnoxious weed

  • Wangchuk, Kesang
    • Journal of Animal Science and Technology
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    • v.57 no.11
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    • pp.34.1-34.5
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    • 2015
  • Himalayan dock (Rumex nepalensis) was evaluated for forage value and antinutrients under three, five and seven weeks cutting intervals in the temperate environment. Dry matter (DM) content was measured for each cutting interval. Forage quality parameters such as Crude Protein (CP), Acid Detergent fiber (ADF), Neutral Detergent Fiber (NDF), Calcium (Ca) and Phosphorus (P) were analyzed. Plants with seven weeks cutting interval gave higher DM yield. CP and P content were significantly higher for three weeks cutting intervals. Average CP contents were 31.38 %, 30.73 % and 27.32 % and average P content 0.58 %, 0.52 % and 0.51 % for three, five and seven weeks cutting intervals, respectively. Ca content did not differ significantly between cutting intervals. The average Ca content were 0.91 %, 0.90 % and 90 %, for three, five and seven weeks cutting intervals, respectively. Tannin and mimosine contents were not significantly different between cutting intervals. Average tannin contents were 1.32 %, 1.27 % and 1.26 % and mimosine 0.38 %, 0.30 % and 0.28 % for three, five and seven weeks cutting intervals, respectively. The study concluded that R. nepalensis could be a potential source of protein for livestock. The study also suggests seven weeks harvesting interval to provide plants with high dry matter yield, high forage quality and very low levels of anti-nutrients.

Effects of Sowing Date and Method on the Yield and Winter Survival of Italian Ryegrass in Paddy (파종시기와 파종방법이 답리작 Italian ryegrass의 월동력과 수량에 미치는 영향)

  • 고영두;정길영;류영우;김두환;김재황
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.11 no.1
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    • pp.30-37
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    • 1991
  • This experiment was conducted to determine optimal sowing date and method of Italian ryegrass(Lo1iuwr wzultiflorum. Tetrone) in paddy of Gyeong Nam area. Treatments given were four sowing dates(Sep. 30, Oct. 10, Oct. 20 and Oct. 30) and four sowing methods (broadcasting on tillage ridging, broadcasting on zerotillage ridging, boundless broadcasting and alternated drilling). The characteristics of growth, winter survival, yield(fresh and dry matter) and nutr~ent quality were observed. The results obtained are summarized as follows: 1. Winter survival and yield components such as plant height, and number of stem were the highest due to higher survival tillers at the sown time of Sep. 30(p<. 05). 2. Plants sown on Sep. 30 showed the highest fresh and dry matter yield. Yield was decreased as delaying the date of sowing. Fresh and dry matter yield sown on Oct. 10, were obtained 7600 and 1100 kg, respectively, aftenvardly sign~ficantly decreased(p< .05). 3. Effect of sowing time and method on chemical composition were not recognized. 4. Winter surviva: sown on boundless-broadcasting was lower(p<. 05) than those of sown on broadcasting on ridging and alternated-drilling. 5. Fresh and dry matter y~elds were the highest when sown on broadcasting on tillage ridging but that of sown on houndless-broadcasting was relatively low. 6. The growth and forage yield were enhanced by sowing up to Oct. 10 and by sown on broadcasting on ridging (tillage or zerotillage).

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Effect of Grass Filter Strips on PO4-P and Soil Loss in Runoff from Forage Cropland (사료작물 재배지에서 초지식생대가 PO4-P 및 토양유실 저감에 미치는 영향)

  • Jo, Nam-Chul;Yoon, Sei-Hyung;Kim, Ki-Young;Lee, Ki-Won;Kim, Meng-Jung;Yook, Wan-Bang;Jung, Min-Woong
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.30 no.4
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    • pp.309-316
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    • 2010
  • The performance of grass filter strips (GFS) in reducing $PO_4$-P concentrations and soil loss from the forage cropland was tested in an experiment on the 10% slope in Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration (RDA) from October 2007 to September 2009. Forage cropland with rye-corn double cropping system receiving inorganic fertilizer or livestock manure (LM) were compared in a natural condition. The plots were hydrologically isolated in a randomized block layout of 3 treatments $\times$ 2 factors $\times$ 3 replicates. Main plots consisted of the length of GFS, such as $25\;m^2$ ($5{\times}5\;m$), $50m^2$ ($5{\times}10\;m$), $75m^2$ ($5{\times}15\;m$). Sub plots consisted of the type of LM, such as chemical fertilizer (CF), cattle manure (CM) and swine manure (SM). Concentrations of $PO_4$-P in surface runoff water were reduced as the length of GFS increased. Especially, GFS with 10 m and 15 m reduced $PO_4$-P concentrations significantly compared to that with 0 m (p<0.05). However, there was not significant different between $PO_4$-P concentrations of GFS with 10m and that of GFS with 15 m. Moreover, Soil loss in surface runoff water were reduced as the length of GFS increased. GFS with 15 m reduced soil loss significantly compared to that with 5 m and 10 m (p<0.05). The results from this study suggest GFS improve the removal and trapping $PO_4$-P and soil loss from forage cropland.

The Effect of Feeding Order on Dry Matter Intake and Ruminal Characteristics Chage of Daily Cattle (젖소에 급여하는 사료의 급여 순서가 사료섭취량 및 반추위내 생리적 변화에 미치는 영향)

  • 윤상기;김현섭;권응기;강우성;차영호
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.16 no.3
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    • pp.245-252
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    • 1996
  • This experiment was carried out to investigate the effect of feeding order bay-concentrate-silage, concentratehay- silage, silage-concentrate-hay, silage-concentrate-hay, concentrate-silage-hay) on ruminal characteristics change with 3 fistulated dry Holstein cows in a Latin square design. The main results obtained were as follows : DM intake in forage hayconcentrate-silage feeding order was 1.65% of body weight, which is the highest of all treatments, but that in concentrate-hay-silage 1.4%, which is the lowest. The difference between max and min rumen pH in hayconcentrate-silage feeding order was lowest as 0.55, but there is no significant. The mean rumen $NH_3N$ content in silage-concentrate-hay feeding order was highest as 6.12mg/100ml, but that in silageconcentrate-hay feeding order lowest as 4.82mg/100ml. Acetic acid and propionic acid content was highest in forage hay-concentrate-silage feeding order. but there is no significant. The ratio of acetic acid to propionic acid averaged 3.47~3.69(NS). In conclusion, the best feeding order fitted in ruminal physiology was forage hay-concentrate-silage.

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Influences of Exposures on Dry Matter Yields and Nutrient Contents of Grasses II. Comparative studies of south and north exposure on nutrient contents of temperate grasses (경사방향이 목초의 수량 및 양분함량에 미치는 영향 II. 주요 목초에 대한남북향사면의 양분함량비교)

  • 박근제;이필상;신재순;정연규
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.7 no.2
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    • pp.97-102
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    • 1987
  • To find out the nutrient contents of temperate grasses on the south and north slope, a field experiment was conducted with five forage species of Dactylis glomerata, Festuca arundinacea, Phleum pratense, Agrostis gigantea and Trifolium repens. It was treated by randomized block design with 4 replications and lasted from September, 1983 to October, 1986 in Suweon. The results obtained are summarized as follows: 1. Mean chemical compositions except NFE and crude fiber of the forage species on the south slope were a little more contented than that of north. But all crude components were significant difference among the species on the south and north exposure. 2. Production of TDN (total digestible nutrient), StE (starch equivalent) ME (metabolizable energy) and NEL (net energy lactation) of different grass species on the north slope were much more increased by 5-30% per ha (Dactylis glomerata by 5-876, Festuca arundinacea by 5%, Phleum pratense by 24-26% and Agrostis gigantea by 25-30%) than that of same grasses on the south, however, those of Trifolium repens were much more increased by 4 1-42% per ha in the south. 3. Mineral contents, Ca/P ratio and K/ (Ca + Mg) equivalent ratio of the species were slightly different between the exposures. But considering the factors of occuring grass tetany, those of the forage species on the north slope were better than that of same species on the south.

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Current Status of Forage Use on the Goat Farming in Mountainous Pasture (흑염소 방목초지의 사초생산성 및 사료가치 평가에 관한 연구)

  • Moon, Sang Ho;Kim, Sang Woo;Choi, Gi Jun;Jang, Se Young;Park, Jae Hyun;Jeon, Byong Tae;Kim, Myoung Hwa;Kim, Sung Jin;Oh, Mi Rae
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.35 no.2
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    • pp.112-118
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    • 2015
  • This study was carried out to offer basic information for the promotion of the goat industry and the improvement of mountainous-pasture management by investigating seasonal changes in forage and livestock productivity according to the grazing-pasture type. The forage productivity of rangeland was the highest (p < 0.05) in summer and decreased in autumn; but that of pasture was the largest (p < 0.05) in spring and had relatively stable productivity with minor seasonal differences, although it decreased slightly in autumn. The dry matter content was not seasonally different at pasture, but it was changeable from spring to autumn at rangeland. The crude protein content increased in autumn at pasture (p < 0.05), and decreased in summer and autumn compared with spring at rangeland. The crude fiber content was lower and the ether extract was higher at pasture compared with rangeland. The average daily gain of the goats was different depending on forage productivity and pasture type; consequently, the lowered forage productivity at rangeland compared with pasture means that rangeland needs to be changed or improved to a pasture type that provides a more effective grazing system for goats.

Effect of Forage Sources in Total Mixed Ration (TMR) on in vitro Rumen Fermentation of Goat (다양한 조사료를 이용해 제조한 TMR이 흑염소 반추위 in vitro 발효성상에 미치는 영향)

  • Lee, Jinwook;Lee, Sung-Soo;Kim, Chan-Lan;Choi, Bong-Hwan;Lee, Sang-Hoon;Kim, Dong-Kyo;Lee, Eun-Do;Kim, Kwan-Woo;Ryu, Chae Hwa
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.41 no.2
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    • pp.102-109
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    • 2021
  • In this study, the effect of forage sources in the total mixed ration (TMR) on in vitro goat rumen fermentation was investigated. Rice straw (RS), Italian ryegrass (IRG), timothy (TIM), and alfalfa (ALF) were used as forage sources. Each forage source was mixed with a commercial goat concentrate diet in the ratio of 1:1. Total 4 TMR were prepared. Rumen simulated in vitro fermentation using goat rumen fluid collected from the slaughterhouse was conducted until 72th. For fermentation parameters, gas production (GP), volatile fatty acids (VFAs), and ammonia nitrogen (NH3-N) were examined. All assays were performed at 24th, 48th, and 72th h of incubation individually. Contents of crude protein and non-fibrous carbohydrate were greater in the order of RS < IRG < TIM < ALF. Significant treatment effects were found in valerate and NH3-N at 24th h of incubation (p<0.05). ALF showed the greatest contents of them and RS was the lowest. At 48th incubation, a significant effect was detected at GP (p<0.05) and RS was greater than others. However, GP of RS was lower than others at 72th. Significant effects on Total VFA, butyrate, and valerate productions were found at 72th h of incubation (p<0.05). ALF showed the greatest production. Methane production from all treatments was not significantly different for each incubation time (p>0.05). The present study provided primary information on how goat rumen fermentation responds to different nutrient contents and forage sources of TMR. And the information could be used for the design or optimizing economical diet formulation for goats.