• The Journal of the Korea institute of electronic communication sciences
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• v.13 no.6
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• pp.1417-1424
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• 2018

The damage caused by foot-and-mouth disease and AI has been repeated in year by year, and the actual damage is held by farmers. Currently, domestic livestock farmers' demand for foreign labor force is rapidly increasing, but the foreign management system is very inadequate, autonomous disinfection consciousness is poor and seasonally disinfest of winter is a serious situation. Thus, the exit management system, where small and medium scale farmers can purchase and install, is necessary for medium and small scaled farmer who is suffering from the pollutants and who does not have capacity to install expensive sterilization. In this paper, we propose ICT fusion combined plasma access control system which can utilize the principle of low temperature plasma lamp, to introduce disinfection system, to ensure convenience and safety that enables integrated control.

• Korean Journal of Environmental Agriculture
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• v.23 no.3
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• pp.123-127
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• 2004

In many organisms, trehalose has been Down as an energy source and a protectant against various environmental stresses such as desiccation, freezing, heat and osmotic pressure. Previously, we have isolated and characterized the genes encoding trehalose-6-phosphate synthase (ZrTPS1) and trehalose-6-phosphate phosphatase (ZrTPS2) from one of the most osmotolerant yeasts, Zygosaccharomyces rouxii. We have also generated transgenic plants by co-introduction of ZrTPS2 and ZrTPS2 into potato plant (ZrTPS2-2A-ZrTPS1 plant) in an attempt to metabolically engineer trehalose in the transgenic plant using the foot-and-mouth disease virus(FMDV) 2A system and to generate drought resistant crop plants. In this research, we assayed previously generated the ZrTPS2-2A-ZrTPS1 plant biofunctionally by drought treatment, and measured the amount of trehalose in the ZrTPS2-2A-ZrTPS1 transgenic plants. The ZrTPS2-2A-ZrTPS1 transgenic plant showed strong drought resistance in spite of little or no accumulation of transgenic in he transgenic plant compared with control plant.

• The Journal of the Korea institute of electronic communication sciences
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• v.14 no.6
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• pp.1215-1220
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• 2019

Currently, each farm bears both the outbreak of foot-and-mouth disease and the damage caused by AI. In addition, complaints about odors in the livestock industry are constantly being recovered and are expected to occur in the future. The purpose of this study is to improve the indoor air quality of enclosed facilities such as barns, houses, pigsty, and etc. This paper develops low-temperature plasma waste heat ventilation system to be installed in ventilation unit location and standardizes heat exchange element, low-temperature plasma lamp, and ballast for enhanced air cleaning function. In addition, this study intends to develop a new control system so that the farmers can connect with existing weather systems, flow fans, and other facility equipment by incorporating ICT.

• Pediatric Infection and Vaccine
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• v.20 no.2
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• pp.81-88
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• 2013

Purpose : This study was performed to investigate the epidemiology of enterovirus (EV) infections in children at a secondary hospital during recent 5 years. Methods : We collected the cerebrospinal fluid, stool and throat swab samples from the pediatric patients with suspected EV infections in KEPCO Medical Center, Seoul, Korea from July 2006 to September 2010. EV detection and genotype identification were performed by RT-PCR at Korea Centers for Disease Control and Prevention. Results : A total of 386 samples were collected from 277 patients during study period. Ninety-eight patients (35.4%) were diagnosed with EV infections. The RT-PCR positive rate was the highest in throat swab samples (48.3%). The median age of patient was 4.7 years (range, 0.1-12.5 years). Aseptic meningitis (50, 51.0%) was the most common clinical manifestation; herpangina (22, 22.4%) and hand-foot-mouth disease (18, 18.4%). One hundred EVs were isolated from 98 patients and 20 genotypes of EV were identified; Echovirus 30 (28 cases, 28%), Enterovirus 71 (12 cases, 12%), Echovirus 25 (10 cases, 10%), Echovirus 9 (9 cases, 9%) and Coxsackievirus A6 (8 cases, 8%). Aseptic meningitis caused by Echovirus 30 was the most common manifestation in 2008. There was no complicated case caused by Enterovirus 71. Conclusion : This study showed the epidemiology of confirmed EV infection in children from 2006 to 2010. There is a need for continuous surveillance of EV infections and its clinical manifestations.

• Journal of Veterinary Science
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• v.22 no.6
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• pp.87.1-87.12
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• 2021

Background: African swine fever virus (ASFV), classical swine fever virus (CSFV), and porcine reproductive and respiratory syndrome virus (PRRSV) are still prevalent in many regions of China. Co-infections make it difficult to distinguish their clinical symptoms and pathological changes. Therefore, a rapid and specific method is needed for the differential detection of these pathogens. Objectives: The aim of this study was to develop a multiplex real-time quantitative reverse transcription polymerase chain reaction (multiplex qRT-PCR) for the simultaneous differential detection of ASFV, CSFV, and PRRSV. Methods: Three pairs of primers and TaqMan probes targeting the ASFV p72 gene, CSFV 5' untranslated region, and PRRSV ORF7 gene were designed. After optimizing the reaction conditions, including the annealing temperature, primer concentration, and probe concentration, multiplex qRT-PCR for simultaneous and differential detection of ASFV, CSFV, and PRRSV was developed. Subsequently, 1,143 clinical samples were detected to verify the practicality of the assay. Results: The multiplex qRT-PCR assay could specifically and simultaneously detect the ASFV, CSFV, and PRRSV with a detection limit of 1.78 × 10⁰ copies for the ASFV, CSFV, and PRRSV, but could not amplify the other major porcine viruses, such as pseudorabies virus, porcine circovirus type 1 (PCV1), PCV2, PCV3, foot-and-mouth disease virus, porcine parvovirus, atypical porcine pestivirus, and Senecavirus A. The assay had good repeatability with coefficients of variation of intra- and inter-assay of less than 1.2%. Finally, the assay was used to detect 1,143 clinical samples to evaluate its practicality in the field. The positive rates of ASFV, CSFV, and PRRSV were 25.63%, 9.36%, and 17.50%, respectively. The co-infection rates of ASFV+CSFV, ASFV+PRRSV, CSFV+PRRSV, and ASFV+CSFV+PRRSV were 2.45%, 2.36%, 1.57%, and 0.17%, respectively. Conclusions: The multiplex qRT-PCR developed in this study could provide a rapid, sensitive, specific diagnostic tool for the simultaneous and differential detection of ASFV, CSFV, and PRRSV.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2014.05a
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• pp.295-298
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• 2014

In recently, a climatic change(such as subtropical climate and frequent unusual high temperature) and the open-trade policies of agricultural & livestock products are increasing the outbreak risk of highly pathogenic avian influenza(HPAI) and foot and mouth disease(FMD), and accordingly the socio-economic damage and impacts are also increasing due to the cases such as damage from the last 5 times of FMD outbreak(3,800 billion won), from 10 years public control cost of Pine Wilt Disease (PWD)(238.3 billion won), and from the increased invasive pests of exotic plant like isoptera. Therefore, the establishment of new operation strategy of IoT(Internet of Things) based satellite early warning system(SEWS) for plants and animals as a subsystem of national disaster response and management system is being required, where the forensic technology & measures should be applied as a government policy to estimate the post compensation and to carry out the legal responsibility.

• Journal of Animal Environmental Science
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• v.17 no.1
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• pp.23-32
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• 2011

The purpose of this study is development of the sprayer that can effectively control pathogens. Image processing was used to analyze the sprayer. Experimental paper in the form of $5{\times}7$ 10 m intervals total of 35 sheets were installed. Experiment used binarized image data obtained by sprayed pigment, to analysis spray volume and distance. The experimental results show that 60 m distance was available to the spray. And spray rate was high up to 30 m. It can be used in livestock farms are expected.

• Journal of Biosystems Engineering
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• v.43 no.3
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• pp.247-254
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• 2018

Purpose: Although previous studies have performed on-farm evaluations for the control of airborne diseases such as foot-and-mouth disease (FMD) and influenza, disease control during the process of livestock and manure transportation has not been investigated thoroughly. The objective of this study is to predict common patterns of livestock-vehicle movement. Methods: Global positioning system (GPS) data collected during 2012 and 2013 from livestock vehicles on Jeju Island, South Korea, were analyzed. The GPS data included the coordinates of moving vehicles according to the time and date as well as the locations of livestock farms and manure-keeping sites. Data from 2012 were added to Esri software ArcGIS 10.1 and two approaches were adopted for predicting common vehicle-movement patterns, i.e., point-density and Euclidean-distance tools. To compare the predicted patterns with actual patterns for 2013, the same analysis was performed on the actual data. Results: When the manure-keeping sites and livestock farms were the same in both years, the common patterns of 2012 and 2013 were similar; however, differences arose in the patterns when these sites were changed. By using the point-density tool and Euclidean-distance tool, the average similarity between the predicted and actual common patterns for the three vehicles was 80% and 72%, respectively. Conclusions: From this analysis, we can determine common patterns of livestock vehicles using previous year's data. In the future, to obtain more accurate results and to devise a model for predicting patterns of vehicle movement, more dependent and independent variables will be considered.

• Korean Journal of Veterinary Service
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• v.45 no.2
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• pp.117-124
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• 2022

In the event of an outbreak of a livestock epidemic, it has been considered that the existing burial-centered carcass disposal method should be improved ecofriendly for prevention of leachate and odors from burial basically in regard of pathogen inactivation. Therefore, the aim of this study is whether it was possible to treat the carcass of cattle and chickens using the chemical carcass treatment method. It was conducted to establish detailed treatment standards for the chemical treatment method of cattle and chicken carcasses based on the results of the proof of the absence of infectious diseases in cattle chickens. After inoculating cattle carcass with 10 pathogens (foot and mouth disease virus, bovine viral diarrhea virus, Mycobacterium bovis, Mycobacterium avium subsp. Paratuberculosis, Brucella abortus, Bacillus anthracis, Clostridium chauvoei, Clostridium perfringens, Escherichia coli, and Salmonella Typhimurium) and chicken carcasses with low pathogenic avian influenza virus, Clostridium perfringens type C, E. coli and Salmonella Typhimurium, these were treated at 90℃ for 5 hours in a potassium hydroxide liquid solution corresponding to 15% of the body weight. This method liquefies all cadaveric components and inactivates all inoculated pathogens by PCR and culture. Based on these results, it was possible to prove that chemical treatment of cattle and chicken carcasses is effective in killing pathogens and is a safe method without the risk of disease transmission. The chemical treatment method of livestock carcasses can be suggested as an alternative to the current domestic burial-centered livestock carcass treatment method, preventing environmental pollution, and contributing to public health.

• Korean Security Journal
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• no.42
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• pp.229-249
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• 2015

As the post-cold war era and globalization go on, national security problems which were not traditional national security problems such as terrorism, crime, environmental disasters, economic crises, cyber-terrorism, diseases, and energy problems threat humanity and nations and demands changes. Also, with the change, the concept of "big government" has emerged as the role of nation expanded. The modern society sees every country change from small government to big government in order to realize the establishment of welfare state. A comprehensive interpretation of security is needed in order to comprehensive protection of citizens beyond outside invasion such as crime, new disaster, terrorism. In Korea, incidents such as Cheonan-Ham, Yeonpeyong-Do, foot-and-mouth disease, Ddos terrorism, pirates hostages, mad cow disease, AI are happening and the humanitarian support for North Korea and the summit of South and North Korea are at a standstill. Also, National emergency management system, comprehensive emergency management center, countrol tower, national security system, cooperation with citizens, establishment of legal and institutional system are needed. The importance of this research is on the reestablishment of new national security and emergency management system according to the comparison between the national security and counter-terrorism system of Korea and that of the United States which is a leading country in this field. Also, the establishment of national emergency management act is needed as a statute for effective function as currently various laws and administrative organizations are dispersed.