• Journal of Genetic Medicine
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• 제5권1호
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• pp.1-6
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• 2008

Knowledge of developmental biology is essential for clinicians who seek to develop a rational approach to the diagnostic evaluation of patients with birth defects. After an accurate diagnosis, a clinician can make predictions about prognosis, recommend management options, and provide an indication of recurrence risk for the parents and relatives. In this paper, we first review the basic mechanisms of embryological development and clinical dysmorphology. We then review cellular and molecular mechanisms in development and related congenital anomalies. Developmental anomalies have a major impact on public health. Genetic counseling and prenatal diagnosis, with the option to continue or to terminate a pregnancy, are important for helping families faced with the risk of a serious congenital anomaly in their offspring. Moreover, primary prevention of birth defects, for example, supplementation of prenatal folic acid and prevention of consumption of alcohol which has teratogenic effects, can be accomplished using developmental biology knowledge.

• 대한한방내과학회지
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• 제20권1호
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• pp.274-279
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• 1999

In order to look about the effect and possibility of oriental medical diagnosis and treatment of pancytopenia of myelodysplastic syndrome. For a patient who has pancytopenia. we diagnose him as myelodysplastic syndrome by CBC, LFT, Ultrasonic wave, Vitamin B12 test, Bone marrow test, Folic acid test and doing Herb-medication, Moxa-Tx continually, we observed the change of WBC, RBC. HGB. MCV, Platelet. ESR with following up CBC test ten times Finally, WBC increased by 900, RBC increased by 400000, MCV was reduced by 7fl. HGB increased by 1.3g/dl. Hct increased by 3.2%, ESR was reduced from 127 to 35mm/h. We diagnosed myelodysplastic syndrome as 'CONSUMPTIVE DISEASE' in oreintal medicine. The treatment effect of pancytopenia was achieved by Herb-medication and Moxa-Tx for invigorating qi, enriching the blood, invigorating the spleen and the kidney-yang, Now we need the study of oriental medical treatment for Aplastic Anemia and AML which is similar this.

• 생약학회지
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• 제48권4호
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• pp.289-297
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• 2017

This study was performed to investigate the effect of 8 weeks administration of snail extract on free radical formation in old female rats (27 weeks). Rats administrated orally with snail extract at the dose of 100 mg/kg, 200 mg/kg, chondroitin sulfate 10 mg/kg and 0.9% saline (control) for 8 weeks. Hematologic profiles and hepatic enzymes were all normal. Results of analysis on snail extract were naicin, Na, protein, sugar, beta-carotene, vitamin (A, B1, B2, B6, C, E), folic acid, phosphorus, lipid, K, cholesterol, chondroitin. Hepatic lipid peroxidase content was decreased, aldehyde oxidase was decreased, glutathione peroxidase and glutathione-S transferase were not changed, but xanthine oxidase, catalase and superoxidase activities were significantly increased in snail extract fed group (p<0.001). Therefore, as the result of this study, it could be expected that the administration of snail extract for 8weeks is the potential to be use as a hepatic anti-oxidative agent.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2001년도 International Symposium on Dietary and Medicinal Antimutgens and Anticarcinogens
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• pp.9-9
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• 2001

Several micronutrients (vitamins and minerals) are required as co-factors in DNA synthesis, DNA repair, DNA methylation and apoptosis. Some notable examples include (a) folic acid and vitamin B12 required for maintenance methylation of DNA and the synthesis of dTTP from dUTP, thus prevent the misincorporation of uracil into DNA, a highly mutagenic and chromosome-breaking event, (b) niacin, is essential in the form of the coenzymes NAD and NADP which act as a substrate for polyADPribose polymerase (PARP), an enzyme thought to facilitate efficient DNA repair and telomere length regulation and (c) zinc, apart from its antioxidant role as a co-factor in Cu/Zn SOD, it is required in its stabilizing role of the DNA-binding domain of p53 (residues 102-292) and thus is essential for apoptotic response to DNA damage. (omitted)

• 대한의생명과학회지
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• 제14권4호
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• pp.203-210
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• 2008

One of cell surface receptor proteins, human folate receptor (hFR) involves in the uptake of folates through cell membrane into cytoplasm, and is anchored to the plasma membrane by a fatty acid linkage, which has been identified in some cells as a glycosylphosphatidylinositol (GPI)-tailed protein with a molecular mass of about 40 kDa. The hFR is released by phosphatidylinositol phospholipase C (PI-PLC) because it contains fatty acids and inositol on the GPI tail. Caveolin decorates the cytoplasmic surface of caveolae and has been proposed to have a structural role in maintaining caveolae. It is unknown whether caveolin is involved in targeting, and is necessary for the function of GPI-tailed proteins. To compare the ability of folic acid binding, internalization and expression of hFR, and the effect of caveolin at the both apical and basolateral side of cell surfaces in Chinese hamster ovary (CHO) clone cells overexpressed the hFR and/or caveolin. Our present results suggest a possibility that the overexpression of caveolin does not be involved in expression of hFR, but plays a role as a factor in PI-PLC releasing kinetics, and for a regulation of formation, processing and function of hFR in CHO clone cells overexpressed cavcolin.

• Preventive Nutrition and Food Science
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• 제7권1호
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• pp.12-17
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• 2002

Simultaneous determination of nine water-soluble vitamins contained in multi-nutrient tablets was carried out by reversed phase high-performance liquid chromatography (RP-HPLC) equipped with analytical $C_{18}$ column and UV (270 nm) detector. Those standard vitamins were successfully separated within 23 minutes by gradient elution with solvent A (0.5 M potassium phosphate monobasic) and solvent B (0.25 M potassium phosphate monobasic-methanol, 1:1). Calibration curves showed good linealities with correlation coefficients (> 0.92) in tested ranged respectively. The detection limits were considered to be 2.1 ng for ascorbic acids 60 ng for Vit B$_{6}$ 3 ng for p-aminobenzoic acid, 9 ng for niacinamide, 9 ng for thiamin, 5.0 ng for folic acid and 1.5 ng for riboflavin at 0.05 a.u.f.s. Solid phase extraction through Sep-Pak (C$_{18}$ ) cartridge was successfully applied for purification of water soluble vitamins in commercial multi-nutrient tablets.ts.

• Preventive Nutrition and Food Science
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• 제13권2호
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• pp.95-103
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• 2008

The aim of this study was to compare nutrient intakes, dietary habits, and blood components of smokers with non-smokers in the Seoul area and its vicinity. The results showed that non-smokers had higher intakes of brown rice, grains, fruit, vegetable and kimchi than the smokers. Smokers consumed more protein (p<0.001), vitamin B1 (p<0.001), vitamin B2 (p<0.007), niacin (p<0.0001), zinc (p<0.031) and phosphorus (p<0.005) than did non-smokers, whereas non-smokers' intakes of vitamin A (p<0.037), and folic acid (p<0.043) was higher than that of smokers. Individuals who smoked tended to have significantly higher levels of hemoglobin and monocytes. There were no significant differences by smoking status for dihydrolipoic acid (DHLA), arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which were in normal ranges. No significant differences by smoking status were shown for plasma homocysteine, HDL-cholesterol, LDL-cholesterol, vitamin C, and vitamin A. In conclusion, because smokers maintain a less healthy diet and life-style, it is to be recommended that educational programs be developed for smokers, guiding them into adopting better dietary habits in order to maintain and improve their health.

• Journal of Dairy Science and Biotechnology
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• 제36권1호
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• pp.1-13
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• 2018

Kefir exhibits antimicrobial activity in vitro against gram-positive and gram negative bacteria, as well as some fungi. The ability of LAB to inhibit the growth of closely related bacteria is well known. This inhibition of pathogenic and spoilage microbes may be due to the production of organic acids, hydrogen peroxide, acetaldehyde, diacetyl, carbon dioxide, or bacteriocins. Lactobacilli are the major contributors to acid production and, hence, a determining factor in the flavor development in kefir. Lactic acid, proteolytic activity, and acetaldehyde are the essential flavor compounds in kefir. Both acid and bacteriocins contribute to the antimicrobial activity of kefir and kefir grains. Kefir is rich in proteins, calcium, vitamin $B_{12}$, niacin, and folic acid. Many studies have investigated the benefits of consuming kefir and have shown that it is a natural probiotic, which when consumed regularly, can help relieve intestinal disorders, promote bowel movement, reduce flatulence, and improve the overall health of the digestive system. Tibetan kefir, which is different from traditional kefir, is produced in China. It has been reported to exhibit antimicrobial activity that is nearly identical to that of traditional kefir. Kefir production is considered a rapidly growing food industry in China.

• 한국식품영양과학회지
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• 제38권4호
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• pp.451-461
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• 2009

본 연구는 뇌성마비 아동의 성장발달에 기여할 간식개발을 위한 기초자료를 수집하기 위해 만 $1{\sim}7$세의 뇌성마비아동과 어머니 각각 99명을 대상으로 뇌성마비 아동의 신체발육과 식습관, 영양섭취상태, 간식섭취실태를 조사한 결과는 다음과 같았다. WLI(Weight-Length Index)를 기준으로 비만도를 판정한 결과 저체중아비율은 45.5%, 정상아는 45.5%, 과체중아는 6.0%, 비만아는 3.0%로 나타났다. Waterlow 분류법을 이용하여 영양불량상태의 정도를 분류 해 본 결과 정상인 아동이 76.6%, 쇠약인 아동이 10.3%, 성장부진인 아동이 8.0%, 성장부진과 쇠약인 아동이 5.0%로 나타났다. 아동들의 영양섭취실태를 알아보기 위해 아동들이 식사 섭취한 내용을 한국인 일일 영양권장량과 비교분석한 결과 아연과 엽산의 섭취량 비율(%RI)은 모든 연령대에서 권장섭취량에 미달하였다. 권장섭취량의 75% 이하를 섭취하는 아동의 비율은 엽산(76.8%)과 아연(76.8%), 열량 (59.8%), 칼슘(52.4%), 철(52.4%)의 경우 50% 이상이었고, 권장섭취량의 125% 이상을 섭취하는 아동들의 비율이 단백질은 85.4%, 비타민 B6은 53.7%로 나타났다. 어머니들이 주로 구입하는 시판간식은 우유 및 유제품(43.5%)과 과일류 (33.3%)가 많았고 시판 간식에 대해서는 첨가물(보존료, 색소)을 가장 문제점으로 생각하고 있었다. 아동들을 위해서 성장발달에 도움이 되는 간식(50.5%)이 새로 개발되기를 가장 바라고 있어서 이에 대한 요구도가 가장 높았으며, 새로개발되기를 바라는 간식의 종류로는 떡류(47.5%)와 과자류 (24.2%) 및 빵류(22.3%)를 선호하였다. 본 연구결과를 통하여 뇌성마비 아동은 정상아동에 비해 성장이 느리고 영양소섭취량도 현저히 낮은 것을 알 수 있었다. 본 연구대상 아동들이 현재의 식품 섭취량으로만 계속 섭취한다면 정상적인 성장을 할 수 없을 것이다. 따라서 뇌성마비 아동과 같이 저영양 상태에 있는 아동의 성장발달과 건강증진을 위한 간식제품의 개발이 시급한 것으로 보인다. 새로 개발될 뇌성마비 아동을 위한 간식모형 도출결과 간식제품은 권장섭취량과 비교했을 때 가장 섭취가 부족한 것으로 나타난 아연과 엽산을 보충하여 성장발달에 보조역할을 할 수 있고 아동들의 저작기능을 향상시키고 손에 잡기 쉬운 형태인 떡의 형태가 가장 적합한 형태로 도출되었으므로 향후 이러한 간식 개발에 대한 연구가 필요하다. 본 연구의 제한점은 뇌성마비 아동의 식사 섭취 조사의 어려움으로 인하여 일일의 식품섭취량만을 조사하여 보고한 것이라 할 수 있다. 그리고 아연과 같은 미량영양소의 영양 상태를 정확하게 측정할 수 있는 생화학적 조사가 더 이루어져야 할 것이다.

• 대한한의학회지
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• 제30권2호
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• pp.56-62
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• 2009

Objectives: We tried to clarify the relationship between lifestyle and plasma homocysteine concentration. We analyzed the data of patients with acute ischemic stroke from the Hanbang stroke registry supported by the Ministry of Science and Technology of Korea. Methods: 458 subjects were recruited from the patients admitted to the Internal Medical Department of Kyunghee University oriental medical center, Dongguk University Ilsan oriental medical center, Kyungwon University Songpa oriental medical center and Kyungwon University Incheon oriental medical center from April 2007 to August 2008. We divided the subjects into four groups by quartiles of plasma homocysteine concentrations and compared the first and last quartile groups. Results: 1. In univariate analysis, the high homocysteine concentration group showed higher rate of older age, male gender, smoking, drinking and lower values in vitamin B12, folic acid, creatinine than the control group with statistical significance. 2. In multivariate analysis, older age, male gender smoking and folic acid showed a close relationship with statistical significance (each OR=3.620, OR=4.218, OR=3.558, OR=0.789). Conclusions: According to the analysis, the relationship between high homocysteine concentration and smoking in patients with acute ischemic stroke were clarified. We hope to standardize and establish criteria on homocysteine in Korea by large epidemiological survey. These study results could be utilized in the future as a basis material.