Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

Simultaneous Determination of the Water Soluble Vitamins in Multi-Nutrient Tablets by Reversed-Phase High-Performance Liquid Chromatography

  • Published : 2002.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Simultaneous determination of nine water-soluble vitamins contained in multi-nutrient tablets was carried out by reversed phase high-performance liquid chromatography (RP-HPLC) equipped with analytical $C_{18}$ column and UV (270 nm) detector. Those standard vitamins were successfully separated within 23 minutes by gradient elution with solvent A (0.5 M potassium phosphate monobasic) and solvent B (0.25 M potassium phosphate monobasic-methanol, 1:1). Calibration curves showed good linealities with correlation coefficients (> 0.92) in tested ranged respectively. The detection limits were considered to be 2.1 ng for ascorbic acids 60 ng for Vit B$_{6}$ 3 ng for p-aminobenzoic acid, 9 ng for niacinamide, 9 ng for thiamin, 5.0 ng for folic acid and 1.5 ng for riboflavin at 0.05 a.u.f.s. Solid phase extraction through Sep-Pak (C$_{18}$ ) cartridge was successfully applied for purification of water soluble vitamins in commercial multi-nutrient tablets.ts.

Keywords

References

  1. Method of Vitamin Analysis Freed,M.
  2. The vitamins v.VII Gyorgy,P.;Pearson,W.N.
  3. J Assoc Off Anal Chem v.67 Simultaneous determination of thiamine and riboflavin in foods by liquid chromatography Augustin,J.
  4. J Chromatogr v.318 Simultaneous analysis of thjamin and riboflavin in foods by high performance liquid chromatography Wimalasiri,P.;Wills,R.B.H. https://doi.org/10.1016/S0021-9673(01)90708-3
  5. J Assoc Off Anal Chem v.70 Evaluation of selected enzymes for thiamin determination Defibaugh,P.W.
  6. J Food Sci v.47 Simultaneous determination of thiamin and riboflavin in selected foods by high performance liquid chromatography Fellman,J.K.;Artz,W.E.;Tassinari,P.D.;Cole,C.L.;Augustin,J. https://doi.org/10.1111/j.1365-2621.1982.tb12942.x
  7. J Assoc Off Anal Chem v.68 Liquid chromatographic determination of thiamin in infant fomula productsby using ultraviolet detection Ayi,B.K.;Yuhas,D.A.;Moffett,K.S.;Joyce,D.M.;Deangelis,N.J.
  8. J Food Sci v.45 Thiamin and riboflavin analysis by high performance liquid chromatography Kamman,J.F.;Labuza,T.P.;Warthesen,J.J. https://doi.org/10.1111/j.1365-2621.1980.tb07548.x
  9. J Chromatogr Sci v.11 Analysis of water soluble vitamins by high speede ion-exchange chromatography Williams,R.C.;Baker,D.R.;Schmit,J.A. https://doi.org/10.1093/chromsci/11.12.618
  10. J Assoc Off Anal Chem v.67 Liquid chromatographic assay of ascorbic acid, niacinamide, pyridoxine, thiamin and riboflavin in multivitamin-mineral preparation Lam,F.L.;Holcomb,I.J.;Fusari,S.A.
  11. J Chromatogr v.231 Thiamin analysis and separation of thiamin phosphate esters by high-performance liquid chromatography Hilker,D.M.;Clifford,A.J. https://doi.org/10.1016/S0378-4347(00)81868-X
  12. J Chromatogr v.83 Application of liquid chromatography in food analysis Van de Weerdhof,T.;Wiersum,M.L.;Reissenweber,H. https://doi.org/10.1016/S0021-9673(00)97062-6
  13. J Food Sci v.43 Assessment of storage effects on vitamin $B_6$ stability and bioavailability in dehydrated food systems Gregory,J.F.;Kirk,J.R. https://doi.org/10.1111/j.1365-2621.1978.tb07418.x
  14. J Chromatogr v.188 Separationof vitamin $B_6$ components by high-performance liquid chromatography Lim,K.L,;Young,R.W.;Driskell,J.A. https://doi.org/10.1016/S0021-9673(00)88444-7
  15. J Agric Food Chem v.28 Comparison of high-performance liquid chromatographic and Saccharomyces uvarum methods for the determination of vitamin $B_6$ in the fortified breakfast cereals Gregory,J.F. https://doi.org/10.1021/jf60229a029
  16. J Chromatogr v.284 High-performance liquid chromatographic analysis of thiamin in rice flour with fluorimetric post-column derivatization Ohta,H.;Baba,T.;Suzuki,Y. https://doi.org/10.1016/S0021-9673(01)87829-8
  17. J Chromatogr Sci v.11 Analysis of water soluble vitamins by high speed ion exchange chromatography Williams,R.C.;Baker,D.R.;Schmit,J.A. https://doi.org/10.1093/chromsci/11.12.618
  18. J Nutr Sci Vitamol v.21 Semiautomated system for analysis of vitamin $B_6$ complex by ion exchange column chromatography Yasumoto,K.;Tadera,K.;Tsuji,H.;Mitsuda,H. https://doi.org/10.3177/jnsv.21.117
  19. J Agric Food Chem v.28 Sulfosalicylic acid as an extraction agent for vitamin $B_6$ in food Vanderslice,J.T.;Maire,C.E.;Doherty,R.F.;Beecher,G.R. https://doi.org/10.1021/jf60232a019
  20. Fresenius Z Anal Chem v.314 Bestimmung von nicotinsaure in fruchtsaften durch HPLC mit elektrochemischer detektion an einer stationaren quecksilbertropfelektrode Kral,K. https://doi.org/10.1007/BF00481844
  21. J Assoc Off Anal Chem v.60 High presure liquid chromatographic analysis of food for folates Clifford,C.K.;Clifford,A.J.
  22. J Agric Food Chem v.34 Simultaneous analysis of ascorbic and dehydroascorbic acid by high-performance liquid chromatography with post column derivatization and UV absorbance Kacem,B.;Marshall,M.R.;Mattews,R.F.;Gregory,J.F. https://doi.org/10.1021/jf00068a030
  23. J Agric Food Chem v.29 Rapid high-performance liquid chromatographic determination of ascorbic acid and combined ascorbic acid-dehydroascorbic acid in beverages Dennison,D.B.;Brawley,T.G.;Hunter,G.L.K. https://doi.org/10.1021/jf00107a009
  24. Food Chem v.15 The HPLC analysis of thiamin and riboflavin in potatoes Finglas,P.M.;Faulks,R.M. https://doi.org/10.1016/0308-8146(84)90037-2
  25. J Food Sci v.49 Influence of initial riboflavin content on retention in pasta during photodegradation and cooking Furuya,E.M.;Warthesen,J.J. https://doi.org/10.1111/j.1365-2621.1984.tb10375.x
  26. J Liquid Chromatogr v.5 The determination of folic acid (pteroylmonoglutamic acid) in fortified products by reversed phase high pressure liquid chromatography Hoppner,K.;Lampi,B.J. https://doi.org/10.1080/01483918208060626
  27. J Liquid Chromatogr v.7 Determination of folic acid in commercial diets by anion-exchange solid-phase extraction and subsequent reversed-phase HPLC Schiefer,G.W.;Wheeler,G.P.;Cimino,C.M. https://doi.org/10.1080/01483918408067033
  28. Proceedings of the kellogg nutrition symposium The importance of vitamins to human health Wiggins,R.A.
  29. J Agric Food Chem v.32 Simultaneous determination of pyridoxine, riboflavin, and thiamin in fortified cereal products by high-performance liquid chromatography Wehling,R.L;Wetzel,D.L. https://doi.org/10.1021/jf00126a027
  30. J Liquid Chromatogr v.8 A sensitive high-performance liquid chromatography method for analyzing riboflavin in fresh fruits and vegetables Watada,A.E.;Tran,T.T. https://doi.org/10.1080/01483918508074084
  31. J Agric Food Chem v.33 Determination of vitamin B-6 in foods and other biological materials by paired-ion high-performance liquid chromatography Gregory,J.F.;Feldstein,D. https://doi.org/10.1021/jf00063a010
  32. Liquid chromatographic analysis of food and beverages v.1 Rouseff,R.;Charalambous,G.(ed.)
  33. J Liquid Chromatogr v.3 Determination of niacin in cereal samples by HPLC Tyler,T.A.;Shrago,R.R. https://doi.org/10.1080/01483918008060170
  34. J Agric Food Chem v.32 Comparison of high-performance liquid chromatographic and microbiological method for the determination of niacin in foods Van Nieker,P.J.;Smit,S.C.C.;Strydom,E.S.P.;Armbruster,G. https://doi.org/10.1021/jf00122a032
  35. Anal Chem v.48 High-pressure liquid chromatographic determination of ascorbic acid in selected foods and multivitamin products Sood,S.P.;Sartori,L.E.;Wittmer,D.P.;Haney,W.G. https://doi.org/10.1021/ac60370a025
  36. Food Chem v.14 HPLC assay of ascorbic acid in fresh and processed fruit and vegitables Rizzolo,A.;Forni,E.;Polesello,A. https://doi.org/10.1016/0308-8146(84)90058-X
  37. HPLC in food analysis Determination of vitamins Van Nieker,P.J.;MaCrae,R.(ed.)
  38. J Assoc Off Anal Chem v.71 Comparison of paired-ion liquid chromatographic method with AOAC fluoremetric and microbiological methods for riboflavin determination in selected foods Reyes,E.S.P.;Norris,K.M.;Tayor,C.;Potts,D.
  39. J Food Sci v.58 Vitamin $B_6$ in raw and fried chicken by HPLC Olds,S.J.;Vanderslice,J.T.;Brochetti,D. https://doi.org/10.1111/j.1365-2621.1993.tb04311.x

Cited by

  1. Screening for folic acid content in vitamin-fortified beverages vol.17, pp.11, 2006, https://doi.org/10.1016/j.foodcont.2005.06.009
  2. Vitamin C content and antioxidant activity of the fruit and of the Ayurvedic preparation of Emblica officinalis Gaertn. vol.104, pp.1-2, 2006, https://doi.org/10.1016/j.jep.2005.08.065