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Light-Dependent Chilling Injury on the Photosynthetic Activities of Cucumber Cotyledons (저온처리한 오이의 자엽에서 광합성 활성의 광의존성 저해)

  • 김현식
    • Journal of Plant Biology
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    • v.36 no.2
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    • pp.133-140
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    • 1993
  • The photosynthetic activities in relation to oxygen evolution rates, quantum yield, CO2 uptake rates and room temperature chlorophyll fluorescence were investigated in cotyledons of cucumber seedlings exposed to low temperature (at 4$^{\circ}C$) for 24 h. Light-chilling caused more inhibition on light-saturated maximum oxygen evolution rates, quantum yield, and CO2 uptake rates than dark-chilling did in the cucumber plant. Light-chilling induced more marked increase in Fo and decrease in (Fv)m/Fm than dark-chilling did in the room temperature chlorophyll induction kinetics. The above results affected by chilling in the light are considered to be associated with the partial damage of the reaction center of PS II and the decreased photosynthetic activities. There occurred a large decrease in qQ with little change in qNP in the light-chilling plant. When light- and dark-chilled plants were recovered at room temperature for 24 h and their chlorophyll fluorescences were induced with light doubling technique, light-chilled plants showed more smaller magnitude and rate of fluorescence relaxation than dark-chilled plants. These suggest that light-chilling might cause some alterations in transthylakoid pH formation, and that photosynthetic apparatus of cucumber cotyledons is more susceptible to light-chilling. In the fast fluorescence induction kinetics, FR was decreased by 60% in the light-chilled plants with reference to $25^{\circ}C$ light-grown plants, while the dark-chilled plants showed a decreased rate of only 20% with reference to $25^{\circ}C$ dark-treated plants for 24 h, indicating that cucumber seedling is very sensitive to chilling stress. So, it is certain that chilling injury to the photosynthetic apparatus is strongly dependent on the presence of light in cucumber seedlings.

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Screening of Natural Compounds for Cancer Prevention by Cytotoxicities and AP-1 Reporter Gene Activities (천연물 지표성분들의 세포독성 및 AP-1 활성 평가를 통한 암예방 기능성 탐색)

  • Choi, Bu-Young;Cho, Seok-Cheol
    • Journal of Convergence for Information Technology
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    • v.7 no.6
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    • pp.89-95
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    • 2017
  • Cancer-inducing PMA stimulates cells to increase the expression of transcription factor c-Jun/c-fos and then increase the activity of AP-1 in the nucleus. The activity of AP-1 has been reported to cause cancer. In this study, We conducted cytotoxicity experiments to assess the safety of natural marker compounds and also observed inhibition of activator protein(AP-1) activity to predict cancer-preventing effects. The results of this experiment indicated that arctigenin, manassantin A, and B can predict the development of cancer prevention agents.

The a-Si:H/poly-Si Heterojunction Solar Cells

  • Kim, Sang-Su;Kim, do-Young;Lim, Dong-Gun;Junsin Yi;Lee, Jae-Choon;Lim, Koeng-Su
    • Journal of Electrical Engineering and information Science
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    • v.2 no.5
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    • pp.65-71
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    • 1997
  • We present heterojunction solar cells with a structure of metal/a-Si:H(n-i-p)/poly-Si(n-p)/metal for the terrestrial applications. This cell consists fo two component cells: a top n-i-p junction a-Si:Hi cell with wide-bandgap 1.8eV and a bottom n-p junction poly-Si cell with narrow-bandgap 1.1eV. The efficiency influencing factors of the solar cell were investigated in terms of simulation an experiment. Three main topics of the investigated study were the bottom cell with n-p junction poly-Si, the top a-Si:H cell with n-i-p junction, and the interface layer effects of heterojunction cell. The efficiency of bottom cell was improved with a pretreatment temperature of 900$^{\circ}C$, surface polishing, emitter thickness of 0.43$\mu\textrm{m}$, top Yb metal, and grid finger shading of 7% coverage. The process optimized cell showed a conversion efficiency about 16%. Top cell was grown by suing a photo-CVD system which gave an ion damage free and good p/i-a-Si:H layer interface. The heterojunction interface effect was examined with three different surface states; a chemical passivation, thermal oxide passivation, and Yb metal. the oxide passivated cell exhibited the higher photocurrent generation and better spectral response.

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Voice Analysis before and after Radioactive Iodine Ablation in Patients with Total Thyroidectomy (적갑상선 전절제술 환자의 방사성 동위원소치료 전.후 음성의 변화에 대한 연구)

  • Hong, Ki Hwan;Seo, Eun Ji;Lee, Hyun Doo;Yoon, Yun Sub;Lim, Seok Tae
    • Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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    • v.24 no.1
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    • pp.33-40
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    • 2013
  • Background and Objectives:This study is to objectively compare and analyze the acoustic changes in the patients with total thyroidectomy before and after RI therapy. Subjects and Methods:For this study, a total of 50 patients with total thyroidectomy were participated as subjects. Voice samples were obtained at the time of post-operation (Post-OP), before high-dose radioactive iodine therapy (Pre-RIT), and after high-dose radioactive iodine therapy (Post-RIT). Acoustic analysis, the maximum phonation time and K-VHI (Korea-Voice handicap index) were used for subjective evaluation. Results:According to the comparison analysis of the three periods, mFo (Hz) was significantly reduced in all of the vowels /a/ and /i/ as the hormone was discontinued. This can be related to the reduction in vocal range. As thyroid hormone was discontinued, Shim (%) and APQ (%) values, which are the parameters related to the degree of aggressiveness, showed a significant increase in the middle vowel /a/. As thyroid hormone was discontinued, emotional index was significantly decreased in VHI (voice handicap index). Conclusion:These results can be assumed that thyroid hormone suspension is related to the increased changes in the vocal intensity, the increase in noise and the reduction in vocal range. Emotionally, these data can be assumed that the responsive factors of one's own voice disorders were significantly decreased in the patients with vocal handicap.

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Growth and Photochemical Reactions of South Korea Two Broad-leaved Evergreen Species according to Light Intensity (광량에 따른 자생 상록활엽 2종의 생육 및 광화학반응)

  • Jang, Bo Kook;Lee, Cheol Hee;Oh, Chan-Jin;Cho, Ju Sung
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.57-57
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    • 2019
  • 실내 수준에서 조사되는 광량조건에 따른 자생 상록활엽 2종의 생육과 광화학반응을 조사하였다. 식물재료는 3년생 사철나무(Euonymus japonicus Thunb.) 및 2년생 돈나무[Pittosporum tobira (Thunb.) W. T. Aiton] 실생묘로 유리온실에서 재배하면서 실험에 사용하였다. 실내 광량은 10, 50, 100 및 200 PPFD(${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$)로 달리하여 8주간 재배하였으며, 광주기(12/12 h), 온도($25{\pm}1^{\circ}C$), 습도($55{\pm}3%$) 및 관수(1회/3일)조건은 고정되었다. 생육특성 및 광화학반응의 요인들이 측정되었으며, 동일기간 동안 유리온실에서 재배된 식물을 대조구로 사용하였다. 실험의 결과, 사철나무는 100, 200 PPFD의 광량에서 대조구에 비해 초장, 줄기직경, 엽수 및 엽장의 생육반응이 우수하였으며, 엽록소함량은 100 PPFD 이상의 광량에서 감소하였다. 또한 100 PPFD 이상의 광량에서 스트레스지수(Fm/Fo), 최대양자수율(Fv/Fm) 및 전자전달효율(ETo/RC)이 감소하였으며, 이는 대조구와도 유사한 경향이었다. 돈나무는 모든 광량조건에서 줄기직경과 엽폭을 제외하고 대조구와 관계없이 생육반응이 일정하였다. 그러나 스트레스지수와 최대양자수율은 10 PPFD 처리구에서 가장 낮게 조사되었고, 비광학적 에너지의 손실(DIo/RC)은 2.53으로 가장 높았다.

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Differences on Growth of Two Quercus Species according to Indoor Light Intensity (자생 참나무속 2종의 실내 광량에 따른 생육 차이)

  • Park, Kyungtae;Lee, Cheol Hee;Oh, Chan-Jin;Cho, Ju Sung
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.58-58
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    • 2019
  • 본 연구에서는 자생 참나무속 2종의 몇 가지 저광량 조건하에서 생육 및 엽록소 형광 반응을 조사하였다. 연구의 재료는 참가시나무(Quercus salicina Blume)와 붉가시나무(Q. acuta Thunb.)의 2년생 실생묘를 사용하였다. 다양한 실내 광환경조건을 조사하여 광량을 10, 50, 100 및 200 PPFD (${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$)로 설정하였으며, 실험기간 동안 광주기(12/12 h), 온도($25{\pm}1^{\circ}C$) 및 습도($55{\pm}3%$)를 동일하게 설정하여 총 8주간 재배하였다. 대조구는 유리온실에서 동일기간 동안 재배된 식물을 사용하였다. 연구의 결과, 참가시나무는 50 PPFD 이하에서 전체 식물 중 50%가 잎이 마르며 떨어지는 경향을 보였으며, 100 PPFD에서도 25%의 잎마름이 관찰되었다. 200 PPFD는 줄기직경을 제외한 초장, 엽수, 엽장, 엽폭 및 엽록소 함량(SPAD)이 대조구와 유사하거나 높았으며, 엽록소 형광 반응에서도 모든 측정값이 대조구와 유의한 차이를 보이지 않았다. 붉가시나무는 50 PPFD 이상의 광량에서는 줄기직경, 엽수, 엽장 및 SPAD가 대조구와 유사하거나 높은 결과를 보였으나, 모든 광량조건에서 초장과 엽폭이 대조구에 비해 유의적으로 낮았다. 엽록소 형광 반응은 모든 광량에서 대조구와 비슷한 양상을 보였으나, 10 PPFD는 초기형광값(Fo)과 비광학적 에너지의 손실(DIo/RC)이 대조구에 비해 유의적으로 낮은 결과를 보였다.

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Growth Responses of Two Lauraceae Species under Indoor Light Intensity (실내 광량조건에서 녹나무과 2종의 생육반응)

  • Jang, Bo Kook;Lee, Cheol Hee;Oh, Chan-Jin;Cho, Ju Sung
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.55-55
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    • 2019
  • 본 연구에서는 실내에 조사되는 다양한 광량하에서 녹나무과 목본 2종을 재배하면서 생육반응을 조사하였다. 식물재료는 참식나무[Neolitsea sericea (Blume) Koidz.]와 후박나무(Machilus thunbergii Siebold & Zucc.)의 3년생 실생묘를 사용하였다. 실험공간은 광량을 10, 50, 100 및 200 PPFD (${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$)로 달리하였으며, 광주기(12/12 h), 온도($25{\pm}1^{\circ}C$), 습도($55{\pm}3%$) 및 관수(1회/3일) 조건이 동일하게 유지되는 밀실로 조성되었다. 각 광량조건 하에서 8주간 재배된 식물의 생육특성 및 광화학반응이 조사되었으며, 유리온실에 동일 기간동안 재배한 식물과 비교하였다. 연구 결과, 참식나무는 광량에 관계없이 초장을 제외한 전반적인 생육반응이 유사하였으며, 엽록소함량(SPAD)은 광량이 증가함에 따라 감소하는 경향이었다. 최대양자수율(Fv/Fm) 및 스트레스지수(Fm/Fo)는 200 PPFD에서 유의한 차이를 나타냈으며, 이는 대조구와 유사한 경향이었다. 후박나무는 대조구와 비교하여 모든 광량조건에서 초장이 감소하였으며, 특히 10 PPFD에서는 잎의 탈락에 따른 관상가치가 저하되었다. 엽록소함량은 100, 200 PPFD 광량에서 감소하였으며, 대조구도 유사한 경향을 나타냈다. 또한 유리온실의 대조구와 100, 200 PPFD 광량에서 최대양자수율, 스트레스지수 및 PSII 성능지수(Pi_Abs)의 유의한 차이가 확인되었다.

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The Horizon Run 5 Cosmological Hydrodynamical Simulation: Probing Galaxy Formation from Kilo- to Giga-parsec Scales

  • Lee, Jaehyun;Shin, Jihey;Snaith, Owain N.;Kim, Yonghwi;Few, C. Gareth;Devriendt, Julien;Dubois, Yohan;Cox, Leah M.;Hong, Sungwook E.;Kwon, Oh-Kyoung;Park, Chan;Pichon, Christophe;Kim, Juhan;Gibson, Brad K.;Park, Changbom
    • The Bulletin of The Korean Astronomical Society
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    • v.45 no.1
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    • pp.38.2-38.2
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    • 2020
  • Horizon Run 5 (HR5) is a cosmological hydrodynamical simulation which captures the properties of the Universe on a Gpc scale while achieving a resolution of 1 kpc. This enormous dynamic range allows us to simultaneously capture the physics of the cosmic web on very large scales and account for the formation and evolution of dwarf galaxies on much smaller scales. Inside the simulation box. we zoom-in on a high-resolution cuboid region with a volume of 1049 × 114 × 114 Mpc3. The subgrid physics chosen to model galaxy formation includes radiative heating/cooling, reionization, star formation, supernova feedback, chemical evolution tracking the enrichment of oxygen and iron, the growth of supermassive black holes and feedback from active galactic nuclei (AGN) in the form of a dual jet-heating mode. For this simulation we implemented a hybrid MPI-OpenMP version of the RAMSES code, specifically targeted for modern many-core many thread parallel architectures. For the post-processing, we extended the Friends-of-Friend (FoF) algorithm and developed a new galaxy finder to analyse the large outputs of HR5. The simulation successfully reproduces many observations, such as the cosmic star formation history, connectivity of galaxy distribution and stellar mass functions. The simulation also indicates that hydrodynamical effects on small scales impact galaxy clustering up to very large scales near and beyond the baryonic acoustic oscillation (BAO) scale. Hence, caution should be taken when using that scale as a cosmic standard ruler: one needs to carefully understand the corresponding biases. The simulation is expected to be an invaluable asset for the interpretation of upcoming deep surveys of the Universe.

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Determination of Si/Al Ratio of Faujasite-type Zeolite by Single-crystal X-ray Diffraction Technique. Single-crystal Structures of Fully Tl+- and Partially K+-exchanged Zeolites Y (FAU), |Tl71|[Si121Al71O384]-FAU and |K53Na18|[Si121Al71O384]-FAU

  • Seo, Sung-Man;Lee, Oh-Seuk;Kim, Hu-Sik;Bae, Dong-Han;Chun, Ik-Jo;Lim, Woo-Taik
    • Bulletin of the Korean Chemical Society
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    • v.28 no.10
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    • pp.1675-1682
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    • 2007
  • Large colorless single crystals of faujasite-type zeolite with diameters up to 200 μm have been synthesized from gels with the composition of 3.58SiO2:2.08NaAlO2:7.59NaOH:455H2O:5.06TEA:1.23TCl. Two of these, colorless octahedron about 200 μm in cross-section have been treated with aqueous 0.1 M TlC2H3O2 and KNO3 in order to prepare Tl+- and K+-exchanged faujasite-type zeolites, respectively, and then determined the Si/Al ratio of the zeolite framework. The crystal structures of |Tl71|[Si121Al71O384]-FAU and |K53Na18|[Si121Al71O384]-FAU per unit cell, a = 24.9463(2) and 24.9211(16) A, respectively, dehydrated at 673 K and 1 × 10-6 Torr, have been determined by single-crystal X-ray diffraction techniques in the cubic space group Fd m at 294 K. The two single-crystal structures were refined using all intensities to the final error indices (using only the 905 and 429 reflections for which Fo > 4σ(Fo)) R1/R2 = 0.059/0.153 and 0.066/0.290, respectively. In the structure of fully Tl+-exchanged faujasite-type zeolite, 71 Tl+ ions per unit cell are located at four different crystallographic sites. Twenty-nine Tl+ ions fill site I' in the sodalite cavities on 3-fold axes opposite double 6-rings (Tl-O = 2.631(12) A and O-Tl-O = 93.8(4)o). Another 31 Tl+ ions fill site II opposite single 6-rings in the supercage (Tl-O = 2.782(12) A and O-Tl-O = 87.9(4)o). About 3 Tl+ ions are found at site III in the supercage (Tl-O = 2.91(6) and 3.44(3) A), and the remaining 8 occupy another site III (Tl-O = 2.49(5) and 3.06(3) A). In the structure of partially K+-exchanged faujasite-type zeolite, 53 K+ ions per unit cell are found at five different crystallographic sites and 18 Na+ ions per unit cell are found at two different crystallographic sites. The 4 K+ ions are located at site I, the center of the hexagonal prism (K-O = 2.796(8) A and O-K-O = 89.0(3)o). The 10 K+ ions are found at site I' in the sodalite cavity (K-O = 2.570(19) A and O-KO = 99.4(9)o). Twenty-two K+ ions are found at site II in the supercage (K-O = 2.711(9) A and O-K-O = 94.7(3)o). The 5 K+ ions are found at site III deep in the supercage (K-O = 2.90(5) and 3.36(3) A), and 12 K+ ions are found at another site III' (K-O = 2.55(3) and 2.968(18) A). Twelve Na+ ions also lie at site I' (Na-O = 2.292(10) and O-Na-O = 117.5(5)o). The 6 Na+ ions are found at site II in the supercage (Na-O = 2.390(17) A and O-Na-O = 113.1(11)o). The Si/Al ratio of synthetic faujasite-type zeolite is 1.70 determined by the occupations of cations, 71, in two single-crystal structures.

Presence of Intact Cumulus Cells during In Vitro Fertilization Inhibits Sperm Penetration but Improves Blastocyst Formation In Vitro (돼지 난자의 체외 수정에 있어서 난구 세포의 존재가 정자 침투율 및 배 발육에 미치는 영향)

  • Yong, H.Y.;Lee, E.
    • Journal of Embryo Transfer
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    • v.22 no.1
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    • pp.1-7
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    • 2007
  • This study was conducted to examine the role of intact cumulus cells during in vitro fertilization (IVF) on sperm penetration, male pronuclear (MPN) formation and subsequent embryo development of oocytes matured and fertilized in vitro. Cumulus-oocyte complexes obtained from the slaughtered gilt ovaries were matured for 44 h in TCM199 containing 10% porcine follicular fluid, epidermal growth factor and hormones. After maturation culture, denuded oocytes or oocytes with intact cumulus cells were coincubated with frozen-thawed boar semen for 8h in a modified tris-buffered medium containing 5mM caffeine and 10mM calcium chloride. Putative zygotes were fixed and examined for sperm penetration and MPN formation (Experiments $1{\sim}3$), or cultured in North Carolina State University-23 medium fo. 156 h (Experiment 3). In Experiment 1, sperm penetration was examined after insemination of denuded oocytes and oocytes with intact cumulus cells at the concentration of $7.5{\times}10^5$ sperm/ml. Optimal sperm concentration for IVF of cumulus-intact oocytes was determined in Experiment 2 by inseminating intact oocytes with $2{\sim}5{\times}10^6$ sperm/ml. In Experiment 3, denuded or intact oocytes were inseminated at the concentrations of $7.5{\times}10^5$ and $4.0{\times}10^6$ sperm/ml, respectively, and in vitro embryo development was compared. Sperm penetration was significantly (p<0.01) decreased in cumulus-intact oocytes compared to denuded oocytes (35.2% vs. 77.4%). Based on the rates of sperm penetration and normal fertilization, the concentration of $4.0{\times}10^6$ sperm/ml was optimal for the IVF of intact oocytes compared to other sperm concentrations. The presence of intact cumulus cells during IVF significantly (p<0.05) improved embryo cleavage (48.8% vs. 58.9%), blastocyst (BL) formation (11.0% vs. 22.8%) and embryo cell number $(22{\pm}2\;vs.\;29{\pm}2\;cells)$ compared to denuded oocytes. In conclusion, these results suggest that intact cumulus cells during IVF inhibit sperm penetration but improve embryo cleavage, BL formation and embryo cell number of porcine embryos produced in vitro.