Journal of Convergence for Information Technology (융합정보논문지)

Convergence Society for SMB (중소기업융합학회)
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Screening of Natural Compounds for Cancer Prevention by Cytotoxicities and AP-1 Reporter Gene Activities

천연물 지표성분들의 세포독성 및 AP-1 활성 평가를 통한 암예방 기능성 탐색

  • Choi, Bu-Young (Department of Pharmaceutical Science & Engineering, Seowon University) ;
  • Cho, Seok-Cheol (Department of Food Science & Engineering, Seowon University)
  • 최부영 (서원대학교 제약공학과) ;
  • 조석철 (서원대학교 식품공학과)
  • Received : 2017.11.10
  • Accepted : 2017.12.20
  • Published : 2017.12.31
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Abstract

Cancer-inducing PMA stimulates cells to increase the expression of transcription factor c-Jun/c-fos and then increase the activity of AP-1 in the nucleus. The activity of AP-1 has been reported to cause cancer. In this study, We conducted cytotoxicity experiments to assess the safety of natural marker compounds and also observed inhibition of activator protein(AP-1) activity to predict cancer-preventing effects. The results of this experiment indicated that arctigenin, manassantin A, and B can predict the development of cancer prevention agents.

암을 유발하는 PMA는 세포를 자극하여 전사인자 C-Jun/C-fos의 발현을 증가시키며 핵 내의 AP-1의 활성을 증가하게 되고 결과적으로 각종 암이 발생된다고 보고되고 있다. 본 연구에서는 천연물 지표성분의 안전성을 보기위한 세포독성과 암예방 효과를 예측할 수 있는 Activator protein(AP-1) 활성억제를 관찰하였다. 천연물 지표성분 38종을 대상으로 세포독성과 AP-1 활성억제를 관찰한 결과 섬수의 지표성분인 Bufogein 과 Cinobufagin에서는 세포독성을 보이며 AP-1 활성 억제와 비교 시 5배 정도 차이를 보임을 알 수가 있다. 반면에 Arctigenin, Manassantin A, B에서는 AP-1 활성 억제 농도는 $2{\mu}M$ 이하이면서 세포독성과의 비율은 15배 이상임을 알 수 있다. 본 실험의 결과를 통해 우방자와 삼백초의 지표성분인 Arctigenin, Manassantin A,B는 암 예방 연구의 가능성을 시사하였다.

Keywords

References

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