• Asian Journal of Atmospheric Environment
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• v.5 no.3
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• pp.152-156
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• 2011

Viable bacteria on water-insoluble airborne particles were detected in the urban atmosphere of Kumamoto ($134^{\circ}45'E$, $32^{\circ}28'N$), Japan, in autumn 2008. Airborne particles were collected onto film-covered Cu meshes under clear weather conditions. The samples were stained by fluorescent stains, and then viewed and photographed with an epifluorescent microscope. Non-biological and bacterial parts in particles larger than 0.8 ${\mu}m$ were distinguished by their morphologies, fluorescent colors and fluorescent intensities. Bacterial viable statuses were discriminated according to cell membrane damage. In total, 2681 particles were investigated and it was found that 78 airborne particles were associated with bacteria. Viable bacteria were identified on 48 particles. A few particles carried multiple viable bacteria. These results provide the evidence that airborne particles act as carriers of viable bacteria in the atmosphere.

• Journal of Korean Society of Water and Wastewater
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• v.30 no.1
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• pp.33-40
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• 2016

In this study, a fluorescent silica nano particle is used as the surrogate for challenging test of membrane surface integrity. The particles are functionalized by a fluorescent dying agent so that as an ultraviolet light is imposed a bright fluorescent image from the particles can be taken. If a membrane surface is damaged and has a compromised part larger than the size of surrogate the fluorescent particles would pass through and contained in the permeate. An operator can directly notice whether the membrane surface is damaged or not by detecting a fluorescent image taken from the permeate. Additionally, the size of compromised part is estimated through analysing the fluorescent image in which we surmise the mass of particles included in the permeate by calculating an average RGB value of the image. The pilot scale experiments showed that this method could be applied successfully to determine if a membrane surface had a damaged parts regardless of the test condition. In the testing on the actual damaged area of $4.712mm^2$, the lowest error of estimating the damaged area was -1.32% with the surrogate concentration of 80 mg/L, flux of $40L/m^2/hr$ for 25 minutes of detection. A further study is still going on to increase the lowest detection limit and thus decrease the error of estimation.

• Applied Science and Convergence Technology
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• v.24 no.6
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• pp.284-288
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• 2015

Anionic small molecules are hard to penetrate the cell membranes because of their negative charges. Encapsulation of small molecules into liposome particles can provide target specific delivery of them. In our previous study, siRNA could be efficiently encapsulated into liposome particles using an asymmetric preparation method of liposomes. In this study, the same method was applied for encapsulation of small anionic fluorescent chemicals such as calcein and indocyanine green (ICG). More than 90% fluorescent chemicals were encapsulated in the asymmetric liposome particles (ALPs). No intracellular fluorescent signal was observed in the tumor cells treated with the unmodified calcein/ALPs and ICG/ALPs, whereas the surface modification with a cell-penetrating polyarginine peptide (R8 or R12) allows cellular uptake of the ALPs. The results demonstrate that the ALPs encapsulating small anionic drugs will be useful for target-specific delivery after modification of target-specific ligands.

• Korean Chemical Engineering Research
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• v.56 no.6
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• pp.826-831
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• 2018

This study presents fabrication of bi-compartmental particles labeled by multiple fluorescence. To compartmentalize fluorescent expression at the particle, two fluorescent dyes with less overlap of the excitation and emission spectra are selected. To ensure the fluorescence stability, the fluorescent dyes contain acrylate functional groups in the molecules so that they can be cross-linked together with monomers constituting the particle. Strong fluorescent expression and compartmentalization were observed at the particle fabricated using the selected fluorescent dyes through confocal microscopy. Furthermore, long-term fluorescence stability was verified by measuring fluorescent expression and intensity for 4 weeks. We anticipate that the bi-compartmental particles labeled by multiple fluorescence can be widely used for multi-target drug delivery system, analysis of 3 dimensional Brownian motion, and investigation of 3 dimensional complex self-assembled morphologies.

• Journal of Korean Society of Water and Wastewater
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• v.19 no.1
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• pp.16-24
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• 2005

Mathematical model was developed to verify a sequential particle removal taking place in a granular media gravity filter. Consequential multi-layer filtration cycle model was applied to verify the fraction of filter effluent particles that are filter influent particles that were never removed as well as the fraction of filter effluent particles that were detached after deposition were performed through laboratory experiments. Three sizes of marker particles were injected ahead of the filter column as a pulse in the presence of four sizes of polystyrene particles that were used as a primary source of particles in the raw suspension to investigate particle attachment alone in contrast to net removal from attachment and detachment. Microscopic counting of filter effluent particles was assumed to reflect attachment. Experimental results indicated that particle detachment is significant beginning from the early phase of filtration. For each size of fluorescent microspheres at one filter depth, fluorescent microsphere removal increased with filter runtime to a maximum due to ripening. The detached fraction of effluent particles increased with particle size and filter depth. The presence of detached particles and the increasing fraction of detached particles in deeper bed were confirmed.

• Bulletin of the Korean Chemical Society
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• v.35 no.1
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• pp.35-38
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• 2014

Herein, we report on the preparation of red fluorescent Si nanoparticles stabilized with styrene. Nano-sized Si particles emit fluorescence under UV excitation, which could be used to open up new applications in the fields of optics and semi-conductor research. Unfortunately, conventional methods for the preparation of red fluorescent Si nanoparticles suffer from the lack of a fully-established standard synthesis protocol. A common initial approach during the preparation of semi-conductors is the etching of crystalline Si wafers in a HF/ethanol/$H_2O$ bath, which provides a uniformly-etched surface of nanopores amenable for further nano-sized modifications via tuning of various parameters. Subsequent sonication of the etched surface crumbles the pores on the wafer, resulting in the dispersion of particles into the solution. In this study, we use styrene to occupy these platforms to stabilize the surface. We determine that the liberated silicon particles in ethanol solution interact with styrene, resulting in the substitution of Si-H bonds with those of Si-C as determined via UV photo-catalysis. The synthesized styrene-coated Si nanoparticles exhibit a stable, bright, red fluorescence under excitation with a 365 nm UV light, and yield approximately 100 mg per wafer with a synthesis time of 2 h. We believe this protocol could be further expanded as a cost-effective and high-throughput standard method in the preparation of red fluorescent Si nanoparticles.

• Proceedings of the KSME Conference
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• 2008.11b
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• pp.2700-2703
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• 2008

An experiment on bubble-driven flow was performed in order to understand fundamental knowledge of flow structure around a rising bubble in a stagnant fluid. The measurement technique consists of a combination of the three most often used PIV techniques in multiphase flows: PIV with fluorescent tracer particles, the digital phase separation with a masking technique and a shadowgraphy. The key point of the measurement is that the background intensity of a PIV recording can be shifted to a higher level than a bubble region using a shadowgraphy in order to distinguish from fluorescent particles and a bubble as well. Flow fields were measured without an inaccurate analysis around a fluid-bubble interface by using only one camera simply.

• 한국가시화정보학회:학술대회논문집
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• 2004.12a
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• pp.119-124
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• 2004

Experimental study was conducted to characterize the flow effect of particle migration in a microchannel which can be used to deliver small amount of liquids, drugs, biological agents and particles in microfluidic devices. Fluorescent particles of $1\{mu}m$ diameter were used to obtain velocity profiles of the fluid in which large particles of $10\{mu}m$ diameter were suspended at different volume fraction of 0.6 and $0.8\%$. Measurements were obtained by using micro-PIV system which contains a Nd:YAG laser with a light of 532-nm wavelength, an inverted epi-fluorescent microscope and a cooled CCD camera to record particle images. The volume fraction of $\phi$ and the particle Reynolds number $Re_p$Rep were used as a parameter to assess the influence of the velocity profile of the suspensions. To expect the slip velocity between the particle and fluids, experiments were carried out at low volume fraction. It was shown that the velocity profile was not influenced by Rep but influenced by the volume fraction, which is in similar trend with the previous study.

• 유체기계공업학회:학술대회논문집
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• 2006.08a
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• pp.297-300
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• 2006

This article discusses about the droplet movement on the super-hydrophobic surface by the electro-wetting on dielectric and the effect of particles on the contact angle as well as the movement is investigated. The movement of droplet, driven by the principle of electro-wetting on dielectric, and the effect of particles are experimentally verified according to the driving voltage and different particles concentrations (fluorescent, charged particles). To increase the contact angle, the super-hydrophobic surface is fabricated and applied to the dielectric layer for the EWOD device. Then its performance is verified and discussed.

• Journal of Biosystems Engineering
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• v.24 no.5
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• pp.391-398
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• 1999

The spraying status of the electrostatic sprayer was evaluated by processing surface spraying images of the natural leaves. Water solution of the fluorescent material was used as a spray medium. The image of the lights reflected by fluorescent droplets was captured under UV light using a color CCD camera. Coverage rate, particle density, and the size distribution of particles were analyzed from the surface images of leaves under various spraying conditions such as spraying nozzle angle and object distance. Spraying characteristics of the electrostatic sprayer was evaluated in comparison with the conventional one. In a case of electrostatic sprayer, coverage rate and particle density increased by the average of 1.57times and 1.01times respectively under various nozzle angles and distances. The number of particle under the diameter of 50 ${\mu}{\textrm}{m}$ also increased significantly.