• 제목/요약/키워드: Fluorescence measurement

검색결과 321건 처리시간 0.022초

평면 이미지 기법을 이용한 분무 특성 해석 (The Spray Characterization Using Planar Imaging Technique)

  • 이경진;정기훈;윤영빈;정경석;정인석
    • 대한기계학회논문집B
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    • 제24권1호
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    • pp.93-101
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    • 2000
  • The characteristics of spray nozzle have been quantified with the measurement of fluorescence and Mie scattering images. To correct the attenuation of the incident light sheet, a sequential double-pass light sheet system and the geometrical averaging of two images was implemented. Quantitative mass flux distribution of spray was obtained from fluorescence image. 3-D image is reconstructed using 2-D radial images. Sauter mean diameter (SMD) distribution was determined using the ratio of fluorescence signal intensity and Mie scattering signal intensity and the values were quantified with PDP A data. The measurement of mass flux and SMD using planar imaging technique agee with PDP A data fairly well in the low density region. However, in dense region, there are significant errors caused by secondary scattering. It was found that the planar imaging technique provides many advantages over the point measurement technique, such as PDP A, and can be implemented for quantitative measurement, especially in low density region.

수중 유속 및 유향의 동시 측정을 위한 이미지 분석 기술에 관한 연구 (Image Analysis for the Simultaneous Measurement of Underwater Flow Velocity and Direction)

  • 서동민;오상우;변성훈
    • 센서학회지
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    • 제32권5호
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    • pp.307-312
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    • 2023
  • To measure the flow velocity and direction in the near field of an unmanned underwater vehicle, an optical measurement unit containing an image sensor and a phosphor-integrated pillar that mimics the neuromasts of a fish was constructed. To analyze pillar movement, which changes with fluid flow, fluorescence image analysis was conducted. To analyze the flow velocity, mean force analysis, which could determine the relationship between the light intensity of a fluorescence image and an external force, and length-force analysis, which could determine the distance between the center points of two fluorescence images, were employed. Additionally, angle analysis that can determine the angles at which pixels of a digital image change was selected to analyze the direction of fluid flow. The flow velocity analysis results showed a high correlation of 0.977 between the external force and the light intensity of the fluorescence image, and in the case of direction analysis, omnidirectional movement could be analyzed. Through this study, we confirmed the effectiveness of optical flow sensors equipped with phosphor-integrated pillars.

Stereo-PIV/LIF의 속도장과 농도장 동시측정 기법을 이용한 러쉬톤 교반기내 난류특성에 관한 연구 (A Study on the Turbulent Characteristics of Rushton Turbine Mixer by Simultaneous Measurement of Velocity and Concentration field with Stereo-PIV/PLIF Technique)

  • 민영욱;김윤기;김경천
    • 대한기계학회논문집B
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    • 제28권3호
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    • pp.365-370
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    • 2004
  • Simultaneous measurement with PLIF(Planar Laser-Induced Fluorescence) and Stereo-PIV(Stereo Particle Image Velocimetry) was performed to investigate the structural characteristics of flow field in Rushton Turbine Mixer. Instantaneous 3D velocity fields are measured by two 2K${\times}$2K CCD cameras focused on an object plane with the angular displacement methods while the concentration fields are obtained through the measurement of the fluorescence intensity of Rhodamine B tracer excited by the second pulse of Nd:Yag laser light. Image distortion due to the camera view-angle is compensated by a mapping function. Finally, the spatial structures of turbulent flow around Rushton turbine were identified by the calculation of synchronized data of the velocity field and concentration field.

Stereo-PIV/PLIF의 속도장과 농도장 동시측정 기법을 이용한 러쉬톤 교반기내 난류특성에 관한 연구 (A Study on the Turbulent Characteristics of Rushton Turbine Mixer by Simultaneous Measurement of Velocity and Concentration Field with Stereo-PIV/PLIF Technique)

  • 민영욱;김윤기;김경천
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2003년도 추계학술대회
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    • pp.694-699
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    • 2003
  • Simultaneous measurement with PLIF(Planar Laser-Induced Fluorescence) and Stereo-PIV(Stereoscopic Particle Image Velocimetry) was performed to investigate the structural characteristics of flow field in Rushton Turbine Mixer. Instantaneous 3D velocity fields are measured by two 2K ${\times}$ 2K CCD cameras focused on an object plane with the angular displacement methods while the concentration fields are obtained through the measurement of the fluorescence intensity of Rhodamine B tracer excited by the second pulse of Nd:Yag laser light. Image distortion due to the camera view-angle is compensated by a mapping function. Finally, the spatial structures of turbulent mixing around Rushton turbine were identified by the calculation of cross-correlation fields between the velocity and concentration field.

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Excimer 형광법을 이용한 연료증기 농도측정법에 대한 연구 (Measurement of Fuel Vapor Concentration by Excimer Fluorescence Method)

  • 황승민
    • 한국환경과학회지
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    • 제27권6호
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    • pp.437-445
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    • 2018
  • Laser induced-exciplex-fluorescence (EXCIPLEX) proposed by Melton is used to visualize fuel vapor in spray combustion. However, in the EXCIPLEX method based on TMPD/naphthalene system, the TMPD : naphthalene ratio is strictly restricted to 1 : 9. In addition, fluorescence intensity due to the vapor phase is extremely weak. To overcome these drawbacks, we propose a new laser-induced-excimer fluorescence (EXCIMER) method to visualize the liquid and vapor phases simultaneously. The spatial distributions of liquid and vapor in fuel spray suspended by ultrasonic waves are compared using the EXCIPLEX and EXCIMER methods. The correlation between fuel vapor concentration and fluorescence intensity is experimentally investigated by measuring the fluorescence intensity of saturated vapor formed over liquid fuel at a controlled temperature. These experimental results indicate that the EXCIMER method is effective for evaluating fuel vapor visualization in spray combustion. Furthermore, the quantitative distribution of fuel vapor concentration can be correctly estimated by the EXCIMER method.

생물학적 초미세력 검출을 위한 탄소나노튜브 프로브의 제작 및 기계적 특성 검출 (Fabrication and Mechanical Properties of Carbon Nanotube Probe for Ultrasmall Force Measurement in Biological Application)

  • 권순근;박효준;이형우;곽윤근;김수현
    • 한국정밀공학회지
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    • 제25권5호
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    • pp.140-147
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    • 2008
  • In this study, a carbon nanotube probe (CNT probe) is proposed as a mechanical force transducer for the measurement of pico-Newton (pN) order force in biological applications. In order to measure nantube's displacement in the air or liquid environment, the fabrication of a CNT probe with tip-specific loading of fluorescent dyes is performed using tip- specific functionalization of the nanotube and chemical bonding between dyes and nanotube. Also, we experimentally investigated the mechanical properties of the CNT probe using electrostatic actuation and fluorescence microscope measurement. Using fluorescence measurement of the tip deflection according to the applied voltage, we optimized the bending stiffness of the CNT probe, therefore determined the spring constant of the CNT probe. The results show that the spring constant of CNT probes is as small as 1 pN/nm and CNT probes can be used to measure pN order force.

Design of Optical Biological Sensor for Phycocyanin Parameters Measurement using Fluorescence Technique

  • Lee, Sung Hwa;Mariappan, Vinayagam;Won, Dong Chan;Ann, Myungsuk;Yang, Seungyoun
    • International journal of advanced smart convergence
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    • 제5권2호
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    • pp.73-79
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    • 2016
  • Remote sensing and measurement are of paramount importance of providing information on the state of water quality in water bodies. The formation and growth of cyanobacteria is of serious concern to in land aquatic life forms and human life. The main cause of water quality deterioration stems from anthropogenic induced eutrophication. The goal of this research to quantify and determine the spatial distribution of cyanobacteria concentration in the water using remote sensing technique. The standard approach to measure water quality based on the direct measurement of the fluorescence of the chlorophyll a in the living algal cells and the same approach used to detect the phycobilin pigments found in blue-green algae (a.k.a. cyanobacteria), phycocyanin and phycoerythrin. This paper propose the emerging sensor design to measure the water quality based on the optical analysis by fluorescence of the phycocyanin pigment. In this research, we developed an method to sense and quantify to derive phycocyanin intensity index for estimating cyanobacteria concentrations. The development of the index was based on the reflectance difference between visible light band 620nm and 665nm. As a result of research this paper presents, an optical biological sensor design information to measure the Phycocyanin parameters in water content.

Synthesis of Fluorescent Thiophene-derivatized Pentytiptycenes and Their Aggregate Behaviors

  • 송진우
    • 통합자연과학논문집
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    • 제3권1호
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    • pp.28-32
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    • 2010
  • Thiophene-derivatized pentiptycenes were synthesized and characterized by NMR and UV-Vis spectroscopy. Aggregation behavior of thiophene-derivatized pentiptycenes was monitored by the measurement of fluorescence. Fluorescence intensities for the thiophene-derivatized pentiptycenes and thiophene-derivatized pentiptycenes aggregates were compared. There is no shift in the maximum of the emission wavelength. In the range of water fraction between 20% and 40%, the emission intensity of thiophene-derivatized pentiptycene aggregates remains almost identical. Fluorescence efficiency incresaed by about 5 times higher when the thiophene-derivatized pentiptycenes forms the aggregates in solution.