• 대한치과의사협회지
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• 제55권2호
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• pp.156-164
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• 2017

Purpose: The aim of this in vitro study was to assess changes in remineralization by stimulated human saliva over a short period of 48 hours with quantitative light-induced fluorescence (QLF) technology. Materials and Methods: Bovine incisor surfaces were demineralized for 10 days. Two types of stimulated saliva were collected from 7 healthy persons. 24 hours after tooth brushing (Stimulated saliva group) and immediately after tooth brushing with 1,000 ppm NaF dentifrice (Dentifrice saliva group). The specimens were immersed in saliva and fluorescence images were obtained by QLF-digital (QLF-D $biluminator^{TM}$,) at 2, 4, 6, 12, 24, and 48 hours fluorescence loss (${\Delta}F%$) of the lesions. A paired t-test was performed to assess fluorescence differences between before (${\Delta}F_{baseline}$) and after (${\Delta}F_{treatment\;time}$) the remineralization process. Results: Before the remineralization, the mean ${\Delta}F_{baseline}$ of the initial demineralized specimens was $-18.42{\pm}0.15$ (%). In both groups, the ${\Delta}F$ values obtained at baseline and after 2 hours were statistically significant (P < 0.001), indicating recovery of the lesions by approximately 40% after 2 hours. After 48 hours, remineralization rates were slightly higher (49%) for the stimulated saliva group than for the dentifrice saliva group (41%), but the difference was not statistically significant. Conclusions: With QLF minute degrees of remineralization by saliva can be measured in periods as short as 2 hours. Additionally no significantly higher effects of remineralization were observed in the dentifrice saliva group when compared to the stimulated saliva group.

• 한국진공학회지
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• 제20권6호
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• pp.409-415
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• 2011

현재 개발 중인 휴대용 XRF (X-Ray Fluorescence) 장치의 검출감도를 향상시키기 위한 방법을 논의하였다. 대기 기체분자에 의한 강도 손실을 최소화하기 위하여 시료와 검출기 사이에 Vacuum module을 설치하였다. Vacuum module은 대기기체 또는 He 기체를 채울 수 있도록 고안하였다. 그리고 Vacuum module 내부에 He 기체를 채운 상태, 또는 진공상태에서 검출감도의 변화를 조사하였다. 그 결과 다음 3가지 중요한 결과를 얻었다. 첫 번째는, 낮은 에너지 영역(3~4 keV)에서의 XRF 강도가 2~4배 정도 증가하였다. 검출감도의 향상은 검출시간의 단축을 의미하기 때문에, 휴대용 XRF 장치에 있어서 매우 중요한 결과이다. 두 번째는, x-ray emission line의 에너지가 3 keV 이하인 원소의 검출 가능성을 확인하였다. 세 번째는, He 기체를 채운 vacuum module을 사용하면, 휴대용 XRF 장치에 진공밀폐용기를 쓰지 않으면서도 대기기체분자에 의한 흡수를 최소화할 수 있다는 것이다. 세 가지 모두 휴대용 XRF 장치의 개발에 있어서 매우 의미있는 결과로 판단된다.

• Journal of Genetic Medicine
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• 제7권1호
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• pp.78-81
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• 2010

완간역위는 드물지 않게 관찰되는 이상이며, 일반적으로 표현형 이상을 일으키지 않으나, 불균형 생식자를 생성하여 반복적인 임신 상실의 원인이 될 수 있다. 22번 염색체의 완간역위는 매우 드물며, 지금까지 몇 례만이 보고되어 있다. 저자들은 반복 임신 상실을 보인 inv(22)(p13q12) 보인자 1례를 보고하고자 한다. 환자는 3번의 초기 임신 상실력이 있었고, 이번 임신에서 rec(22)dup(22q)inv(22)(p13q12) mat 염색체 이상으로 인한 태아수종을 경험하였다. 모체의 22번 완간역위와 이로 인한 태아의 재조합 이상은 위치특이 탐색자(TUPLE1 on 22q11.2, ARSA on 22q13)를 이용한 형광제자리부합법으로 증명하였다. 22번 완간역위와 재조합 22번 염색체는 염색체 검사상 쉽게 간과될 수 있는 이상의 하나로, 정확한 진단을 위해서는 추가 분자유전학적 검사를 비롯해 세심한 주의가 필요하다.

• Clinical and Experimental Pediatrics
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• 제58권8호
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• pp.313-316
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• 2015

Interstitial deletions involving the chromosome band 15q22q24 are very rare and only nine cases have been previously reported. Here, we report on a 12-day-old patient with a de novo 15q22q23 interstitial deletion. He was born by elective cesarean section with a birth weight of 3,120 g at 41.3-week gestation. He presented with hypotonia, sensory and neural hearing loss, dysmorphism with frontal bossing, flat nasal bridge, microretrognathia with normal palate and uvula, thin upper lip in an inverted V-shape, a midline sacral dimple, severe calcanovalgus at admission, and severe global developmental delay at 18 months of age. Fluorescence in situ hybridization findings confirmed that the deleted regions contained at least 15q22. The chromosome analysis revealed a karyotype of 46,XY,del(15) (q22q23). Parental chromosome analysis was performed and results were normal. After reviewing the limited literature on interstitial 15q deletions, we believe that the presented case is the first description of mapping of an interstitial deletion involving the chromosome 15q22q23 segment in Korea. This report adds to the knowledge of the clinical phenotype associated with the 15q22q23 deletion.

• Molecules and Cells
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• 제24권1호
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• pp.105-112
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• 2007

Most neuroblastoma cells have chromosomal aberrations such as gains, losses, amplifications and deletions of DNA. Conventional approaches like fluorescence in situ hybridization (FISH) or metaphase comparative genomic hybridization (CGH) can detect chromosomal aberrations, but their resolution is low. In this study we used array-based comparative genomic hybridization to identify the chromosomal aberrations in human neuroblastoma SH-SY5Y cells. The DNA microarray consisting of 4000 bacterial artificial chromosome (BAC) clones was able to detect chromosomal regions with aberrations. The SH-SY5Y cells showed chromosomal gains in 1q12~ q44 (Chr1:142188905-246084832), 7 (over the whole chro-mosome), 2p25.3~p16.3 (Chr2:18179-47899074), and 17q 21.32~q25.3 (Chr17:42153031-78607159), while chromosomal losses detected were the distal deletion of 1p36.33 (Chr1:552910-563807), 14q21.1~q21.3 (Chr14:37666271-47282550), and 22q13.1~q13.2 (Chr22:36885764-4190 7123). Except for the gain in 17q21 and the loss in 1p36, the other regions of gain or loss in SH-SY5Y cells were newly identified.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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• pp.15-15
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• 2002

Telomere erosion is the earliest chromatin modification in cells entering the apoptotic regime. The purpose of this investigation was to demonstrate that loss of telomeric DNA was involved in the induction of mitotic catastrophe and cell death of Chinese hamster Don cells that were grown continuously without subculturing for several days. Don, which is a diploid male Chinese hamster-derived cell line, was grown without sub-culturing for one to four days at 37℃ and then harvested for cytological preparations. (omitted)

• Environmental Analysis Health and Toxicology
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• 제3권3_4호
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• pp.9-15
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• 1988

The word of stress crime from Latin language as stringere and it was used in medical fields from 1935. According to Selye, all the biological bodies reveal physilolgical changes when some stimulation exceed normal levels, and consequently the pituitary gland and adrenal systems are activated. Jacob expressed that stress is the loss of homeostasis by physical, chemical, and emotional stimulation. When biological organisms receive extreme stress the amount of catecholamine excretion are increase. Author investigated the catecholamine contents in rat urine after giving the low temperature stress, noise stress, and water immersion stress. The 24 hours rat urine was collected by adding 1 ml 6 N-HCl and the sample is passed through Bio-Rex 70 samples treatment column to extract catecholamine and detected the catecholamine with HPLC-fluorescence detetor. The highest epinephrine concentration was 67.14 ng in water immersion stress condition and the dopamine concentration of 221.37 ng was shown in the low temperature stress condition.

• BMB Reports
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• 제33권1호
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• pp.43-48
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• 2000

4-Aminobutyrate aminotransferase is a key enzyme of the 4-aminobutyric acid shunt. It converts the neurotransmitter 4-aminobutyric acid to succinic semialdehyde. In order to study the structural and functional aspects of catalytically active Cys residues of pig brain 4-aminobutyrate aminotransferase, we purified the active form in E. coli by coproduction of thioredoxin. The structural arrangement for functional requirements of a dimeric protein using a bifunctional sultbydryl reagent was then characterized, and the spatial proximity between the essential SH groups and a cofactor (pyridoxal-5'-phosphate) binding site was determined. The bifunctional sultbydryl reagent DMDS reacted with the enzyme at the ratio of one molecule per enzyme dimer. This resulted in an approximately 50% loss of enzymatic activity. The spatial proximity of the distance between the essential SH groups and the cofactor-binding site was determined by the energy transfer measurement technique. The result (approximate 20 ${\AA}$) suggested that cross-linking of two sulfhydryl groups with DMDS is not near a PLP binding site.

• 한국식생활문화학회지
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• 제14권5호
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• pp.467-476
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• 1999

We studied to improve the functional properties of soybean protein isolate by dimethylglutarylation and acetylation. Soybean protein isolate was acylated rapidly up to 80% modification and more of 80% modification was proceed slowly. Electrophoretic analysis showed that more changes in modified protein. Also, modification of soybean protein produced more ionizable tyrosines and exposed more hydrophobic groups, while modified protein exhibited a loss of reactive sulfhydryl groups. Spectrophoretic studies demonstrated that the shift was occurred at the UV 278nm and fluorescence 333nm, respectively, and the intensity decreased as the degree of modification was increased.

• International Journal of Advanced Culture Technology
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• 제7권4호
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• pp.260-267
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• 2019

Harvested de-astringent persimmon 'Fuyu' were treated with various lighting sources under low (3℃) and high (22℃) temperatures. The weight loss rate of fruits was lower in those with Red LED than Fluorescence and Blue LED under both temperature conditions. Hardness and soluble solid content of fruits were higher in those with 3℃ / Blue LED or mixed LED (Blue+Red LEDs). Beta-carotene and lycopene content of fruit peel were higher in those with 3℃ than 22℃ and with Red LED or light sources with mixed red wavelength under both temperatures. When the fruits treated with light and temperature were stored for 4 days under 3℃ / dark condition, the hardness of the fruits did not significant difference among the treatments. Taken together all the results, it would be best to treat it light sources mixed red wavelength under 3℃.