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Genome-wide Examination of Chromosomal Aberrations in Neuroblastoma SH-SY5Y Cells by Array-based Comparative Genomic Hybridization  

Do, Jin Hwan (Bio-Food and Drug Research Center, Konkuk University)
Kim, In Su (Department of Biotechnology, Konkuk University)
Park, Tae-Kyu (Department of Biotechnology, Konkuk University)
Choi, Dong-Kug (Department of Biotechnology, Konkuk University)
Publication Information
Molecules and Cells / v.24, no.1, 2007 , pp. 105-112 More about this Journal
Abstract
Most neuroblastoma cells have chromosomal aberrations such as gains, losses, amplifications and deletions of DNA. Conventional approaches like fluorescence in situ hybridization (FISH) or metaphase comparative genomic hybridization (CGH) can detect chromosomal aberrations, but their resolution is low. In this study we used array-based comparative genomic hybridization to identify the chromosomal aberrations in human neuroblastoma SH-SY5Y cells. The DNA microarray consisting of 4000 bacterial artificial chromosome (BAC) clones was able to detect chromosomal regions with aberrations. The SH-SY5Y cells showed chromosomal gains in 1q12~ q44 (Chr1:142188905-246084832), 7 (over the whole chro-mosome), 2p25.3~p16.3 (Chr2:18179-47899074), and 17q 21.32~q25.3 (Chr17:42153031-78607159), while chromosomal losses detected were the distal deletion of 1p36.33 (Chr1:552910-563807), 14q21.1~q21.3 (Chr14:37666271-47282550), and 22q13.1~q13.2 (Chr22:36885764-4190 7123). Except for the gain in 17q21 and the loss in 1p36, the other regions of gain or loss in SH-SY5Y cells were newly identified.
Keywords
Chromosomal Aberration; Comparative Genomic Hybridization; DNA Microarray; Neuroblastoma SH-SY5Y;
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