• Molecular & Cellular Toxicology
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• v.1 no.1
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• pp.32-39
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• 2005

Polycyclic aromatic hydrocarbons (PAHs) are an important class of environmentally prevalent xenobiotics that exert complex effects on the biological system and characterized as probably carcinogenic materials. Single cell gel electrophoresis assays were performed in order to evaluate DNA damage occurring in the T-and B lymphocytes, spleens (T/B-cell), bone marrow, and livers of mouse exposed to mixture of PAHs (Benzo(a)pyrene, Benzo(e)pyrene, Fluoranthene, Pyrene) at dose of 400, 800, or 1600 mg/kg body weight for 2 days. DNA damage of the cells purified from mice was increased in dose dependent manner. In the blood cells and organs, DNA damage was also discovered to vary directly with PAHs. Especially T-cells had been damaged more than B-cell. Plasma proteomes were separated by 2-dimensional electrophoresis with pH 4-7 ranges of IPG Dry strips and many proteins showed significant up-and -down expressions with the dose dependent manner. Of these, significant 4 spots were identified using matrix-assisted laser desorption/ionization-time of fight (MALDI-TOF) mass spectrometry. Identified proteins were related to energy metabolism and signal transduction.

• Ocean and Polar Research
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• v.31 no.2
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• pp.189-198
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• 2009

Passive air samplers with polyurethane foam (PUF) disks were employed to determine seasonal gas phase variation of polycyclic aromatic hydrocarbons (PAHs) in ambient air on Anmyeon island from March 2007 to January 2008. Sum of 13 PAHs ranged between $3.5\;ng/m^3$ and $27.6\;ng/m^3$. Total PAHs during the heating season was 6.2 times higher than non-heating season. The dominant PAHs components during sampling periods were low and middle molecular weight PAHs including phenanthrene, fluoranthene, pyrene and chrysene. Gas exchange fluxes of PAHs across the air-water interface of the Yellow Sea were calculated using a modified two-film exchange model. PAHs fluxes ranged from $196\;ng/m^2/d$ net volatilization during summer to $3830\;ng/m^2/d$ net absorption during winter. Passive air sampler provides a convenient and cost-effective tool for measuring averaged gas phase PAHs, which was successfully used for calculation of gas exchange flux of PAHs in the Yellow Sea.

• Journal of Korean Society for Atmospheric Environment
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• v.7 no.1
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• pp.17-22
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• 1991

Total suspended particulates (TSP) in the atmosphere was collected and size-fractionated by Andersen high volume air sampler for one year (Mar. 1987 $\sim$ Feb. 1988) in Seoul. The concentrations of several polycyclic aromatic hydrocarbons and heavy metals were determined to investigate the atmospheric concentrations, seasonal variations and its relationship with the size distribution of suspended particulate matter. The arithmetic mean concentration of total suspended particulates was 200.44 $\mug/m^3$. The concentrations of heavy metals were 2433.80 for Fe, 629.49 for Zn, 600.71 for Pb, 143.87 for Cu, and 107.21 $ng/m^3$ for Mn, respectively. The concentrations of PAHs were 3.83 for benzo(a) pyrene, 2.95 for benzo(k)fluoranthene, and 4.42 $ng/m^3$ for benzo(ghi)perylene, respectively. PAHs, Pb and Zn abounded in particles below 2.0 $\mu$m, while Fe and Mn aboounded in particles above 2.0 $\mu$m. TSP and its chemical compositions showed the seasonal variations. The concentrations of anthrophogenic pollutants like TSP, PAHs and heavy metals in the fine particles were highest in winter and lowest in summer. PAHs and Pb analyzed showed significant correlations between each other and between TSP concentration in fine particles, indicating that the particles in which they are contained have a similar behavior in the atmosphere.

• Journal of Korean Society for Atmospheric Environment
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• v.20 no.3
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• pp.331-343
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• 2004

The results of individual PAH source profiles that can be applied to receptor model are as follows. The sum of 16 PAH concentrations was 391.41 ng/S㎥ in a tunnel. Phenanthrene was the most abundant compound among 16 PAH, and then pyrene, fluoranthene, anthracene, and naphthalene can be seen in elevated contents. 11,056.61 ng/S㎥ of 16 PAH concentrations in BC oil boiler was two times higher than 6,582.57 ng/S㎥) of those in LNG boiler. Naphthalene was the most abundant compound in both facilities. Phenanthrene, anthracene, and acenaphthylene were the second dominant compound group in order from both facilities. BC oil boiler had relatively high concentration of pyrene compared to LNG boiler that had high concentration of fluorene and did not detect pyrene. The sum of 16 PAH concentrations emitted from MSW incinerators after APCD (air pollution control device) was three times higher than those from MSW incinerators before APCD. However, the concentrations of more than 4-ring PAH compounds (e.g., benzo (a)anthracene) before APCD were higher than those after APCD. This fact implies that PAHs generated by combustion process are eliminated in APCD and they are continuously produced in stack or atmosphere by PAHs precursors.

• Journal of the Korean Chemical Society
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• v.36 no.6
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• pp.933-940
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• 1992

Calix[6]arene react with formaldehyde and secondary amines to yields water soluble Mannich bases which can be converted to the corresponding quaternary salts. Treatment of the quaternary salts with a nucleophile such as cyano, ethoxy, and hydride yields p-substituted calix[6]arenes. Calix[8]arene too react with formaldehyde and diallyl amine to yield a water soluble Mannich base. The transport of neutral arenes through an aqueous phase along a concentration gradient mediated by those of water soluble calixarenes as molecular carrier was studied in a U-type cell. Naphthalene, anthracene, pyrene, and fluoranthene are tested as a neutral solid guest compounds for the transport experiment.

• Journal of Environmental Science International
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• v.12 no.11
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• pp.1145-1157
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• 2003

The surface sediments inside Hallim Harbor, one of the major harbors of Jeju Island, were sampled three times (June, September and December, 2001) and analyzed for 16 polycyclic aromatic hydrocarbons (PAHs), recommended by US-EPA as priority pollutants, to assess their distribution levels and to suggest their possible origins. The concentrations of PAHs ranged from 19 to 496 ng/g on a dry weight basis with a mean value of 245 ng/g, and the levels were low to moderate in comparison with other areas in the world. Based on comparisons of individual and total PAH concentrations with effects-based and equilibrium partitioning-based on sediment quality guidelines, the potential for the biological effects were expected to be low. The sedimentary PAHs may be correlated with organic carbon and mud contents to some extent. From the examinations of the four PAH origin indices, such as LMW/HMW (low molecular weight 2-3 ring PAHs over high molecular weight 4-6 ring PAHs), phenanthrene/anthracene ratio, fluoranthene/pyrene ratio, chrysene/benzo〔a〕anthracene ratio, it can be concluded that the sediment PAH contaminations were ascribed to both pyrolytic and petrogenic origins.

• Environmental Analysis Health and Toxicology
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• v.17 no.3
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• pp.187-195
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• 2002

다환방향족탄화수소류 화합물의 증기상-입자상간의 분배평형의 설명을 위해 흔히 입자상의 흡착지점이 균질하고 총흡착면적은 TSP에 비례한다는 가정을 사용하는데 본 연구의 목적은 이러한 가정의 타당성을 평가하는 것이다. 본 연구를 위해 도심에서 6단의 다단계 대기중입자채집기를 사용하여 대기 중의 입자를 포집하였으며 이들 입자에 흡착된 phenanthrene, anthracene, fluoranthene, pyrene을 분석하여 입경별 분포를 측정하였다. 특히 연구기간 중에 황사현상이 일어나 입경분포나 입자의 기원이 매우 다른 경우에 대한 연구가 가능하였다. 주요연구결과로서 우선 야마사키가 제안한 분배평형의 온도 의존식은 제한된 범위에서 사용되어야 한다는 것이 관측되었다. 즉, 황사현상이 일어나는 경우와 같이 입자의 흡착특성과 입경분포가 보통때와 다른 경우에는 log Kp와 l/T의 관계에서 선형성이 상당히 저하되었다. 또한 특히 낮은 온도에서는 입자의 입경분포가 달라지면 전체적인 분배평형이 달라지게 되는 것으로 평가되었으며 입자의 흡착특성도 분배평형의 온도의존성에 결정적인 영향을 줄 수 있는 것으로 나타났다. 따라서 입자의 기원이 다양하거나 입경분포가 달라지면 흡착평형이 바뀌기 때문에 흡착특성의 균질성과 단순한 TSP를 전제로 하는 분배평형의 평가나 예측은 실제 대기조건에서는 정확하지 않을 수가 있으며 제한적인 조건에서 사용되어야 할 것이다.

• Journal of Environmental Science International
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• v.14 no.4
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• pp.413-425
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• 2005

The 16 priority PAHs (Polycyclic Aromatic Hydrocarbons) designated by US Environmental Protection Agency were analyzed for some digested sludges from wastewater treatment plants in Korea. PAHs are an important group of organic contaminants present in sewage sludge due to their persistence and toxic potential. PAHs were extracted from freeze-dried sludges using a methylene chloride-methanol (2:1) mixture in a soxhlet extractor. The extracts were cleaned-up by silica gel/alumina combination column and subsequently fed into gas chromatograph/mass spectrometer (GC/MS) for determining PAH contents. The sum of the 16 PAHs in the sewage sludge varied from 534.8 to $5754.5 {\cal}ug/{\cal}kg$, dry wt.. In the sewage sludge, phenanthrene appears as the most abundant PAHs, followed by naphthalene, pyrene, fluoranthene. Source of the investigated sewage sludges relatively predominated pyrogenic. PAHs levels of sewage sludges in Korea appeared to be lower than those in other countries.

• Environmental Mutagens and Carcinogens
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• v.24 no.3
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• pp.128-136
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• 2004

CYP1A1 is known to be inducible by xenobiotic compouds such as polyciclic aromatic hydrocarbons(PAHs) and 2,3,7,8-tetrachloro-dibenzo-p-dioxin(TCDD). These chemicals have been identified worldwide and can have a significant impact on the human health and well being of human and wildlife. Given these issues, the detection and quantification of these chemicals in biological, environmental and food samples is important. First, we investigated the effect of on CYP1A1 promoter activity, 7-ethoxyresorufin-O-deethylase(EROD) activity and CYP1A1 mRNA expression induced by benzo(k)fluoranthene(B(k)F) in MCF-7 cells. We found that B(k)F significantly up-regulates the level of CYP1A1 prompter activity, EROD and CYP1A1 mRNA. When cells were treated with genistein, it was not changed that EROD and CYP1A1 mRNA, compared to that of control. However, genistein inhibited the B(k)F-induced CYP1A1 promoter activity and mRNA level at high concentration. Furthermore, in this study, effects of HDAC(histone deacetvlase) inhibitors on human prostate cancer cells proliferation were examined. HC-toxin, SAHA and TSA inhibited cell proliferation in PC3 cells. A novel HDAC inhibitor, IN2001 also suppressed the growth of PC3 cells. And IN2001 and SAHA increased S phase and G2/M phase at 12 hrs treatment but cells were arrested G0/G1 phase at 45 hrs treatment. The HC-toxin treatment for 24 hrs and 48 hrs increased G0/G1 at low concentration ($0.1\mu\textrm{m}$) but increased G2/M at more than concentration of $1\mu\textrm{m}$. TSA increased G2/M phase. These findings height the possbility of developing HDAC inhibitors as potential anticancer therapeutic agents for the treatment of prostate cancer.

• Journal of Korean Society for Atmospheric Environment
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• v.30 no.6
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• pp.519-530
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• 2014

Twenty four individual polycyclic aromatic hydrocarbon (PAH) compounds both in gas- and particle-phase were quantified in three tunnels (Namsan Tunnel 3, Jeongneung Tunnel, Bukak Tunnel) to characterize vehiculate emission of PAHs. Gas phase PAHs were dominant in tunnels which consisted of 85% of total PAHs concentrations. Naphthalene and 2-methyl naphthalene were the most abundant gas phase PAH compounds, while the concentrations of fluoranthene and pyrene were highest in the particle phase. Most (96%) of the gas phase PAH compounds consisted of two- and three-aromatic rings whereas most of the particle phase PAHs were in four and five-rings (67%) in tunnels. Average BaP-eq concentrations of PAHs in the particle phase ($20.8{\pm}11.6ngm^{-3}$) was about twenty fold higher than that in the gas phase ($1.6{\pm}0.6ngm^{-3}$). It means that the particle phase PAHs has more adverse health effect than the gas phase PAHs even though the concentrations of the particle phase PAHs were lower than those of the gas phase PAHs. Compared to previous studies reporting diagnostic ratios for specific PAH compounds, the profile of individual PAH compounds measured in this study reflected well for the vehiculate emissions. We reported, for the first time, on the results of the profile of individual PAH compounds measured in tunnels for both gas and particle phases.