Molecular & Cellular Toxicology

  • 제1권1호
  • /
  • Pages.32-39
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  • 2005
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  • 1738-642X(pISSN)
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  • 2092-8467(eISSN)
대한독성유전 단백체학회 (The Korean Society of Toxicogenomics and Toxicoproteomics)
권호 표지

Effects of Polycyclic Aromatic Hydrocarbons on DNA Damage and Plasma Protein Expression in Mouse

  • Oh, Sang-Nam (Medical Science Research Center, Korea University) ;
  • Oh, Eun-Ha (Medical Science Research Center, Korea University) ;
  • Im, Ho-Sub (School of Public Health, Korea University) ;
  • Jo, Gyu-Chan (Graduate School of Medicine, Korea University) ;
  • Sul, Dong-Geun (Medical Science Research Center, Korea University) ;
  • Kim, Young-Whan (Department of Environmental Health, College of Health Sciences, Korea University) ;
  • Lee, Eun-Il (Medical Science Research Center, Korea University)
  • 발행 : 2005.03.31
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초록

Polycyclic aromatic hydrocarbons (PAHs) are an important class of environmentally prevalent xenobiotics that exert complex effects on the biological system and characterized as probably carcinogenic materials. Single cell gel electrophoresis assays were performed in order to evaluate DNA damage occurring in the T-and B lymphocytes, spleens (T/B-cell), bone marrow, and livers of mouse exposed to mixture of PAHs (Benzo(a)pyrene, Benzo(e)pyrene, Fluoranthene, Pyrene) at dose of 400, 800, or 1600 mg/kg body weight for 2 days. DNA damage of the cells purified from mice was increased in dose dependent manner. In the blood cells and organs, DNA damage was also discovered to vary directly with PAHs. Especially T-cells had been damaged more than B-cell. Plasma proteomes were separated by 2-dimensional electrophoresis with pH 4-7 ranges of IPG Dry strips and many proteins showed significant up-and -down expressions with the dose dependent manner. Of these, significant 4 spots were identified using matrix-assisted laser desorption/ionization-time of fight (MALDI-TOF) mass spectrometry. Identified proteins were related to energy metabolism and signal transduction.

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