• Journal of the Korean Society of Tobacco Science
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• v.31 no.2
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• pp.69-74
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• 2009

Potato Virus Y (PVY), PVY-vein necrosis strain, causes severe damage at growth, yield and leaf quality on flue-cured tobacco in Korea. The development of PVY resistant flue-cured varieties without quality deterioration is therefore urgently desired. The flue-cured tobacco, KF120 (Korea Flue-cured 120), was a male-sterile (ms) $F_1$ hybrid derived from the cross between msKF117 and KF0007-7. msKF117 was developed from the cross of NC82 with N. africana and KF0007-7 was developed from the cross of KF117 with NC82. The agronomic characteristics and disease resistance of KF120 was evaluated during 2006-2007 field performance test. It showed better growth characteristics and yield performance than standard cultivar KF109. It had 2 more leaves per plant, flowered 2 days later than KF109. The yield of cured leaf of KF120 was increased by about 5% compared to KF109. The chemical composition and physical properties of the cured leaf of KF120 were as much acceptable as those of KF109. KF120 showed high resistance to PVY compared to KF109. It showed a similar mode of resistance to bacterial wilt and black shank as was found in KF109.

• Journal of the Korean Society of Tobacco Science
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• v.5 no.2
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• pp.71-75
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• 1983

The relative proportion of the major smoke components in the nonvolatile phase from some varieties of tobacco, 1. e. flue- cured, burley, and aromatic tobacco, were determined. In flue- cured tobacco smoke, hydroquinone and glycerol we re exhibited higher concentrations than in the smoke from the other tobaccos. In aromatic tobacco smoke, the contents of non-volatile phase were present in lower concentrations.

• Journal of the Korean Society of Tobacco Science
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• v.2 no.2
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• pp.58-71
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• 1980

本 硏究는 1979年度 優良黃色種잎담배 (flue-cured tobacco) 導入品種의 地域適應成 試驗을 基礎로하여 Hicks等 16個 品種의 收量, 草長, 葉面積 및 開花期에 대한 地域適應性과 安定性 및 形質相關을 統計的 方法으로 分析 檢討하였다. 回歸係數 (b植)는 適應性을, 決定計係(r2) 및 平均偏差平方和(S2 di)는 安定性의 尺度로 사용하였다. 1.

• Journal of the Korean Society of Tobacco Science
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• v.6 no.1
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• pp.73-78
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• 1984

The changes of contents of organic acids in flue-cured tobacco leaves depending on stalk position and maturity viz immature, mature and overmature were studied in 1982-83. Among them, particularly, the leaves harvested at mature stage have the lowest amounts of oxalic and citric acids in smoking or cutter leaves but the acid contents has increased during the overmature. The linolenic acid content tended to be lower with increase of maturity.

• Journal of the Korean Society of Tobacco Science
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• v.23 no.1
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• pp.12-18
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• 2001

This study was conducted to investigate the effect of tobacco curing on far infrared light on the quality of flue-cured tobacco leaves. Physicochemical characteristics such as alkaloid, total sugar, total nitrogen, starch, total phenolic compounds, browning degrees, and color differences, in cured tobacco leaves were compared between hot air and far infrared curing methods. There was no significant difference in sugar/nicotine ratio and nitrogen number between the curing methods, while the contents of ether extract and filling capacity were increase by far infrared curing. Therefore, it was expected that far infrared curing might be effective for high aroma and physical properties of the cured tobacco leaves.

• Journal of the Korean Society of Tobacco Science
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• v.26 no.1
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• pp.27-34
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• 2004

This study was carried out to examinate the effect of aging period on chromatic, chemical and organoleptic characteristics, and to evaluate of optimum aging period for each grade in flue-cured leaf tobacco. The leaf tobaccos were produced in 2000, and threshed, redried and packed in carton box under the current methods. Four grades of processed leaf(A3O, B1O, C1L and D3L) were stored during 24 months(May 10, 2001 to April 31, 2003) in warehouse of Chungju Leaf Tobacco Processing Factory. The leaf tobaccos were sampled at three month intervals for analysis of chromatic, chemical and organoleptic properties. Yellow(b), pH values and total sugar contents of four grades were significantly decreased during the aging. Filling values, tar, nicotine and CO contents of tobacco smoke, and puff number of cigarettes were not significantly changed during the aging. Positive correlation coefficients were significantly observed between taste and irritation of the calculated attributes from contents of volatile oil components in leaf tobacco and those of the panel sensory attributes. The ratio of maximum change in taste attribute was larger than that in irritation attribute during aging. The optimum aging periods estimated by taste for A3O, B1O, C1L and D3L were 17.8, 14.9, 10.8, and 9.8 months, respectively. The thin leaf(Primings and Cutters) undergo satisfactory aging earlier as compared to bodied leaf(Leaf and Tips). The results suggest that decrease of aging period for thin leaf from 18～21 to 9～12 months may be beneficial to save storage cost in flue-cured tobacco.

• Journal of the Korean Society of Tobacco Science
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• v.28 no.2
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• pp.75-82
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• 2006

This study was carried out to investigate the changes of free sugars, non-volatile organic and fatty acids in flue-cured leaf tobacco during aging. The threshed leaf tobacco(B1O and C1L) produced in 2002 crop year was aged for 21 month in warehouse of Oc-Cheon Leaf Tobacco Processing Factory. The leaf tobacco were sampled at three month intervals for analysis of free sugars, non-volatile organic and fatty acids. The major free sugars of flue-cured were glucose and fructose regardless of tobacco grades. After aging period of 21 months, the contents of glucose and fructose showed a tendency to slightly decrease, and there was no appreciable change in the contents of sucrose in B1O and C1L grades. The major compound of non-volatile organic acid and fatty acid were malic, oxalic and citric, linolenic, linoleic and palmitic acid regardless of tobacco grade. After aging period of 21 months, the contents of malic, malonic, oxalic and fumaric acid showed a tendency to slightly decrease, whereas succinic acid was slightly increased, and maleic acid was not changed in B1O and C1L grades. The decreases in fatty acid contents in B1O and C1L grade tobacco loaves after 21 month aging were 16.5% and 9.8%, respectively. The decreases in linolenic acid contents in two grades were the highest, showing 22.1% and 12.0% reduction after 21 month aging.

• Journal of the Korean Society of Tobacco Science
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• v.17 no.1
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• pp.27-32
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• 1995

A new flue-cured tobacco mutant line BU 8832-85 was developed at Taegu Experiment Station, Korea Ginseng and Tobacco Research Institute in 1994. KF 8832-85 was resulted from a cross of flue-cured cultivars NC 95$\times$NC 2326, and developed by a pedigree system of breeding ; initial selection was made by plant type and resistance to bacterial wilt(BW) disease(heudomonas solanaceamm) in the F2 generation under the natural field conditions infested with the pathogen. One white flowered plant was occurred by spontaneous mutation in a certain line among the F3 generatioin while the others were pink. Six plants from the seeds by selfing were selected at the field infested with the pathogen among 240 populations with white flowering in the F4, KF 8832-85 was selected based on yield and leaf quality trials among 6 lines in Fs generation. BCF 8832-85 was compared with its Parent for certain agronomic and chemical characteristics at Taegu Experiment Station in 1993 and 1994. The results showed that KF 8832-85 have white flower, the stalk height was approximately that of NC 2326 but averaged about loom taller than NC 95. It produced ground suckers as much as NC 95, and did not breakdown leaf at the same as WC 2326. KF 8832-85 have high resistance to bacterial wilt disease. Yield of KF 8832-85 was 10 and 18% higher then that of NC 2326 and WC 95, respectively. Price per Kg was equal to that of NC 2326. The contents of nicotine and reducing sugar did not differ significantly from NC 95, while total nitrogen was significantly lower than NC 95. Therefore, the new mutant line is genetically stable for agronomic and chemical characteristics and provides a source of bacterial wilt disease resistance for use in breeding resistant flue-cured cultivars. Key words : Mutant line, White flower, Spontaneous mutation.

• Journal of the Korean Society of Tobacco Science
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• v.5 no.1
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• pp.35-42
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• 1983

This study was conducted to know changes in chemical components and some enzyme activity on tobacco leaves of NC2326 and Hyangchio (Nicotiana tabacum L. ) by different curing methods. The activity of $\alpha$, $\beta$-amylase and invertase of flue-cured tobacco variety NC2326 were higher than that of sun-cured variety Hyangchio. And the peak of all of enzyme activity in both varieties showed at about 60 hours (at color fixing stage) and 6 days (yellowing stage) after start of flue-curing and sun-curing, respectively. The peak time of reducing sugar content were same with the maximum enzyme activity during curing process in both varieties. During both curing process, the content of starch was greatly reduced and there was a corresponding increase in reducing sugars. Increase in total alkaloids content continued during flue-curing process in both varieties, but during sun-curing process total alkaloids content of NC 2326 were Increased whereas those of Hyangchio were decreased markedly.

• Journal of the Korean Society of Tobacco Science
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• v.27 no.1 s.53
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• pp.100-106
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• 2005

To obtain the optimum condition for analysis of 10 alkaloids in tobacco leaves, such as nicotine, nornicotine, anatabine, anabasine, myosmine, cotinine, 2,3'-dipyridyl, $\beta-nicotyrine,\;\beta-nornicotyrine\;and\;\beta-formylnornicotin$, 5 types of extraction method were investigated by GC-FID and GC/MS. The optimum condition of alkaloid extraction was achieved by using methanol:dichloromethane(1:3, v/v) after NaOH treatment. The use of mass selective detector (MSD) provided unambiguous nicotine related alkaloid analysis. Alkaloids in various tobacco leaves were extracted with the optimum extraction condition and quantified by GC/MS/SIM mode. Compared with concentrations of alkaloids among the various tobacco leaves, the concentration of alkaloids was generally in the order burley > flue-cured > oriental tobacco. In flue-cured tobacco leaves, the order of concentration of alkaloids was nicotine > anatabine > nornicotine > $\beta-nicotyrine\;>\;\beta-formylnornicotine\; >\;myosmine\;>\;2,3'-dipyridyl\;>\;cotinine\;>\;anabasine\;>\;\beta-nornicotyrine$. However, in the case of burley and oriental tobacco leaves, the concentration of nornicotine was higher than that of anatabine.