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Determination of Nicotine and Other Minor Alkaloids in Tobacco Leaves by GC/MS  

Lee Jeong-Min (KT&G Central Research Institute)
Min Hye-Jung (KT&G Central Research Institute)
Kim Yong-Ha (KT&G Central Research Institute)
Rhee Moon-Soo (KT&G Central Research Institute)
Publication Information
Journal of the Korean Society of Tobacco Science / v.27, no.1, 2005 , pp. 100-106 More about this Journal
Abstract
To obtain the optimum condition for analysis of 10 alkaloids in tobacco leaves, such as nicotine, nornicotine, anatabine, anabasine, myosmine, cotinine, 2,3'-dipyridyl, $\beta-nicotyrine,\;\beta-nornicotyrine\;and\;\beta-formylnornicotin$, 5 types of extraction method were investigated by GC-FID and GC/MS. The optimum condition of alkaloid extraction was achieved by using methanol:dichloromethane(1:3, v/v) after NaOH treatment. The use of mass selective detector (MSD) provided unambiguous nicotine related alkaloid analysis. Alkaloids in various tobacco leaves were extracted with the optimum extraction condition and quantified by GC/MS/SIM mode. Compared with concentrations of alkaloids among the various tobacco leaves, the concentration of alkaloids was generally in the order burley > flue-cured > oriental tobacco. In flue-cured tobacco leaves, the order of concentration of alkaloids was nicotine > anatabine > nornicotine > $\beta-nicotyrine\;>\;\beta-formylnornicotine\; >\;myosmine\;>\;2,3'-dipyridyl\;>\;cotinine\;>\;anabasine\;>\;\beta-nornicotyrine$. However, in the case of burley and oriental tobacco leaves, the concentration of nornicotine was higher than that of anatabine.
Keywords
alkaloids; burley; flue-cured; oriental tobacco; GC/MS/SIM;
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