Journal of the Korean Society of Tobacco Science (한국연초학회지)

The Korean Society of Tobacco Science (한국연초학회)
권호 표지

Determination of Nicotine and Other Minor Alkaloids in Tobacco Leaves by GC/MS

GC/MS를 이용한 잎담배 중 알칼로이드 함량 분석

  • Published : 2005.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

To obtain the optimum condition for analysis of 10 alkaloids in tobacco leaves, such as nicotine, nornicotine, anatabine, anabasine, myosmine, cotinine, 2,3'-dipyridyl, $\beta-nicotyrine,\;\beta-nornicotyrine\;and\;\beta-formylnornicotin$, 5 types of extraction method were investigated by GC-FID and GC/MS. The optimum condition of alkaloid extraction was achieved by using methanol:dichloromethane(1:3, v/v) after NaOH treatment. The use of mass selective detector (MSD) provided unambiguous nicotine related alkaloid analysis. Alkaloids in various tobacco leaves were extracted with the optimum extraction condition and quantified by GC/MS/SIM mode. Compared with concentrations of alkaloids among the various tobacco leaves, the concentration of alkaloids was generally in the order burley > flue-cured > oriental tobacco. In flue-cured tobacco leaves, the order of concentration of alkaloids was nicotine > anatabine > nornicotine > $\beta-nicotyrine\;>\;\beta-formylnornicotine\; >\;myosmine\;>\;2,3'-dipyridyl\;>\;cotinine\;>\;anabasine\;>\;\beta-nornicotyrine$. However, in the case of burley and oriental tobacco leaves, the concentration of nornicotine was higher than that of anatabine.

Keywords

References

  1. Andersen, R.A., Fleming, P.D., Burton, H.R, Hamilton-Kemp, T.R, Sutton, T.G. (1991) Nitrosated, acylated, and oxidized pyridine alkaloids during storage of smokeless tobaccos: effects of moisture, temperature, and their interactions. J. Agric. Food Chem. 39: 1280- 12870 https://doi.org/10.1021/jf00007a017
  2. Cai, J., Liu, B., Lin, P., Su, Q. (2003) Fast analysis of nicotine related alkaloids in tobacco and cigarette smoke by megabore capillary gas chromatography. J. Chromatogr. A 1017: 187-193 https://doi.org/10.1016/j.chroma.2003.07.003
  3. CORESTA (2005) Determination of nicotine in tobacco and tobacco products by gas chromatographic analysis. CORESTA Recommended Method No. 62
  4. Health Canada (1999) Determination of alkaloids in whole tobacco. Health Canata T-301
  5. Hecht, S.S., Chen, C.B., Hoffmann, D. (1979) A study of tobacco carcinogenesis. 17. Tobacco-specific nitrosamines: occurrence, formation, carcinogenicity, and metabolism. Accouts Chem. Res. 12: 92-98 https://doi.org/10.1021/ar50135a003
  6. Jeffery, R.N., Tso, T.C. (1955) Tobacco constituents, Qualitative differences in the alkaloids fraction of cured toaccos. J. Agric. Food Chem: 3: 680-682 https://doi.org/10.1021/jf60054a005
  7. Saitoh, F., Noma, M., Kawashima, N. (1985) The alkaloid contents of sixty Nicotiana species. Phytochemistry 24: 477-480 https://doi.org/10.1016/S0031-9422(00)80751-7
  8. U.S. von Euler (1965) Tobacco alkaloids and related compounds. Macmillan, New York, p.37
  9. Wu, W., Ashley, D.L., Watson, C.H. (2002) Det emination of nicotine and other minor alkaloids in international cigarettes by solid-phase microextraction and gas chromatography/mass spectrometry. Anal. Chem. 74: 4878-4884 https://doi.org/10.1021/ac020291p
  10. Yang, S.S., Smetena, I., Huang, C.B. (2002) Determination of tobacco alkaloids by gas chromatography with nitrogen-phosphorus detection. Anal. Bioanal. Chem. 373: 839-843 https://doi.org/10.1007/s00216-002-1380-1
  11. 장기철, 한상빈, 김용옥, 이운철 (1991) 잎담배 alkaloid 분석에 관한 연구. 한국연초학회지 13: 20-26