• Journal of the Korean Society of Tobacco Science
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• v.4 no.2
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• pp.23-30
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• 1982

Six flue- cured cultivars of Nicotiana tabacum L., 15 possible $F_1$ hybrids and $F_2$ Populations among them, and 15 haploid Populations from Fl hybrids and haploids from Parents, were evaluated. Comparisons of the $F_1$, hybrids and $F_2$ Populations with the Parents indicated that heterosis values were small but significant for yield, plant height, days to flower, leaf length and width and total alkaloids from-6.0% to 5.4% in $F_1$ hybrids, and from -3.4% to 3.6% for Plant height days to flower leaves per plant in $F_2$ populations, respectively. There were positive correlations for yield, plant height, days to flower, leaves per plant and total alkaloids between diploid and haploid populations. Increase or decrease ratio of haploids to diploids of total alkaloids was appeared to 30.3%. Those for yield, leaf length and width, value arid reducing sugar were ranged from -4.1% to -27.6%.

• Journal of the Korean Society of Tobacco Science
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• v.26 no.2 s.52
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• pp.186-192
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• 2004

This study was carried out to analyze the organic acids, polyphenols, pigments and fiber materials concentration with a different stalk position and grade of korean leaf tobaccos. Eight kinds of flue-cured leaf tobaccos which were different stalk position and grade were used for this study. Three kinds of major organic acids(citric, malic and oxalic), 2 kinds of polyphenols(chlorogenic acid and rutin), 3 kinds of pigments($\beta$-carotene, chlorophyll-a and chlorophyll-b), and 2 kinds of fiber components(pectin and lignin) were analyzed. All of these chemical components were changed with a different stalk position. When the citric acid, malic acid, $\beta-carotene$, chlorophyll-a, and lignin concentration were low in the middle stalk position and high in both bottom and upper position, oxalic acid and chlorogenic acid show the highest concentration in the middle stalk position. All of these chemical components also changed with a different grade of leaf tobaccos. As the citric acid, malic acid, $\beta-carotene$, chlorophyll-b, and lignin concentration decreased as the grade ascended, the oxalic acid and chlorogenic acid concentration increased as the grade ascended. This results assumed that the quality of korean leaf tobacco was directly proportional to oxalic acid and chlorogenic acid concentration but it was inversely proportional to citric acid, malic acid, $\beta-carotene$, chlorophyll-b and lignin concentration.

• Journal of the Korean Society of Tobacco Science
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• v.13 no.1
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• pp.61-68
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• 1991

The moisture contents of threshed leaf tobacco during aging storage were very important factor for the improvement or deterioration of leaf quality. Decay and carbonization of leaf tobacco were mainly controlled by moisture contents during storage. The influence of moisture contents on the decay and carbonization of threshed leaf tobacco (Flue-Cured, Var. NC 82. AB3O-1 grade) were examined during 1 year. The moisture contents of leaf tobacco were treated to 3 kg as 12% (controle), 16%, 20% and 24% after redrying, respectively, These tobacco were put into center of carton box containing about 200kg with 12%'s tobacco, and then packed and stored from June, 1988 under natural weather condition in warehouse at Ock-Cheon threshing plant. Samples were taken from dissected carton box at 0, 1, 2, 4, 7 and 12 months after moisture treatment and Racking time, and some chemical properties were investigated at every sampling time. In results of inducement to decay and carbonization of leaf tobaccos during 1 year's storage by moisture treatment. decayed leaf was appeared but carbonized leaf did not. Calorification and decayed leaf occurred at high moisture contents above 20%, and these phenomena were appeared between 30 and 150 days after moisture treatment and packing. High moisture content leaves (above 20%) caused nearby other leaves to be sunk with high moisture content. Nicotine and calcium contents were increased to 20%~30% and 10%~22%, respectively, but total sugar contents was decreased to 40%~60% by moisture treatment(20% ~24% ).

• Journal of the Korean Society of Tobacco Science
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• v.9 no.2
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• pp.59-68
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• 1987

This study was conducted to investigate the change of starch, total sugar, nicotine, and total nitrogen contents in green leaves at various stages of maturity(50-106 days after transplanting), and to relate between chemical constituents for flue-cured tobacco (Cv NC82 and BY 4). For the two cultivars and all stalk positions, starch contents increased with maturation, but decreased with senescence. Nicotine contents increased, while total nitrogen contents decreased with delay of harvest. Total sugar contents had a tendency of decrease. For the two cultivars , starch correlated negatively with total nitrogen, and positively with nicotine. Total nitrogen correlated negatively with nicotine. A delay of 5 to 7 days than conventional harvesting time would reach to the better ratio of the reducing sugar to nicotine ratio.

• Journal of the Korean Society of Tobacco Science
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• v.19 no.2
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• pp.102-106
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• 1997

The inluence of styrofoam-panel thickness of floating frame on the seedling growth of flue-cured tobacco(Nicotiana tabacum L.) in the newly developed direct-seed float system was investigated . Floating frame used instead of styrofoam-tray consisted of styrofoam-panel (106×108cm) for floating, eight holes(1.Sx2S.0 cm) to uniform the water, and nutrition supply far seedling and water and nutrition absorption cloth placed on the styrofoam-panel. Each floating-frame may lay eight plastic-trays on it. Eight grades of styrofoam-panel thickness from 26 mm to 46mm and two kinds of media were used in the greenhouse system. Dry cells were found from the styrofoam-panel thickness of 43mm in carbonized chaff, compost and original soil rate of L3-3(v/v) media, and of 37mm in peat and perlite rate of 8-2(v/v) media. The thinner styrofoam-panel produced more tender and succulent seedling with the more trunk and the less root weight. When considering the appearance of dry cell, seedling growth, producing healthy tobacco seedlings, and utilization of styrofoam-panel for two kinds of medias the ideal styrofoam-panel thickness were suggested to be around 34mm for flue-cured tobacco reeling production in the newly developed direct-seeding float system. Key words : tobacco seedlings, float system, medium, styrofoam pannel, dry cell.

• Journal of the Korean Society of Tobacco Science
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• v.28 no.2
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• pp.130-135
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• 2006

This study was carried out to improve the analytical methods for determination of organic acids in tobacco leaf by HPLC. The samples for HPLC analysis were pre-treated by means of SPE. The calibration curve for each acid was linear and $R^2$ values ranged from 0.9990 to 1.0000. The limit of detection were obtained from the calibration curves and their values were between 1.39 to $4.87{\mu}g/mL$. Recovery rates of organic acids were between 88.6 % to 98.5 %. The concentrations of organic acids among the various tobacco leaves were compared to the concentration of organic acids, were in the order oriental, burley, flue-cured tobacco. In the case of flue-cured and oriental tobacco leaves, the order of concentration of organic acids was malic acid, oxalic acid, citric acid. But in the case of burley tobacco leaves, the order of concentration of organic acids was citric acid, oxalic acid, malic acid.

• Journal of the Korean Society of Tobacco Science
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• v.14 no.1
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• pp.12-23
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• 1992

This experiment was conducted to determine the optimum dosage of gamma rays for inducing artificial mutation of several mutant characters in the flue-cured tobacco. 1) In Hicks and BY 104, the gammarays irradiation has no significantly different effect on seed germination from the control. However, the average dosage for 50% growth inhibition was 25-30kr for all the varieties tested, which inhibition 46-52% and 43-57% of the seedling growths for Hicks and BY 104, respectively. 2) A mutant line 83H-5 was selected from Hicks by irradiation gamma ray at the level of 30kr. It has white flower, more resistance to bacterial wilt, Pssudomonas solanacearum, lower plant and stalk height, narrower leaf width, larger leaf shape index(lento width) and later days to flower when compared with the original variety Hicks. 3) White flower was recessive to pink flower in F, and Br (F1 X Hicks) progenies. F2 population of the cross gave segregation ratio of 3 pink flower:1 white flower, and B, (F1 X 83H-5) Population gave 1:1 ratio. Results showed that the white flower character is governed by a single recessive gene.

• Journal of the Korean Society of Tobacco Science
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• v.4 no.1
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• pp.51-59
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• 1982

This experiment was conducted to investigate the effects of fertilization level, additional dressing time and form of nitrogen on the growth and formation of Alkaloid in flue-cured tobacco. The results are abstracted as follows. Length and width of harvested leaves, area of a leaf, leaf area per plant, number of harvested leaves, weight of a leaf, and leaf weight of a plant were larger in the $NO_3$-N plot than $NH_4$-N plot. Length, width and area of the middle leaves increased by additional dressing on 7 days before topping, but the dry weight Per unit leaf area of those was decreased. Leaf shape was broaden in additional dressing plots in comparison with 100% of basal dressing plot, but there is no difference among the additional dressing plots. And the smallest leaf shape index was shown at upper leaves in 100% of basal dressing Plot. Leaf weight of a Plaint was the highest in 12kg/10a Plot among $NO_3$-N form and in 12kg/10a plot among $NH_4$-N form. Total Alkaloid content was higher in $NH_4$-N plot between fertilization forms, and highest in 15kg/10a plot among fertilization levels and at the position of middle and upper leaves in the plot of 7 days before topping among additional dressing times.

• Journal of the Korean Society of Tobacco Science
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• v.4 no.1
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• pp.29-35
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• 1982

Potassium deficiency symptoms were studied with flue-cured tobacco which was applied with different levels of compound fertilizer (10-15-20) ; 75kg, 100hg, and 125kg/10a. Ratio of N/$K_2O$ in leaves was increased from bottom to top stalk position due to the increase of nitrogen content in leaves. Nitrogen content in leaves was increased from stalk to tip as wall as from midrib to laminae, but vice versa in potassium content. Consequently, resulting in potassium deficiency symptoms in tip of leaves. Rate of reabsorption by rainfall during the latter part of growth was highest at top stalk position in case of nitrogen, but lowest in potassium. This observation was more evident with higher application rate of fertilizer. Nitrogen content of about 4 % in leaves of top stalk position applied with 125kg/10a was maintained up to 85days after trans planting. No increase in potassium in upper leaves was observed over the level of 100kg/10a fertilizer application. As the result, N/$K_2O$ ratio in leaves of top stalk position applied with 125kg/10a was kept at more than 1.0 up to 85days after transplanting, but it was less than 0.9 at 65days after tracts planting with less than 100kg/10a fertilizer application.

• Journal of the Korean Society of Tobacco Science
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• v.11 no.2
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• pp.241-246
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• 1989

Mold development on leaf tobacco(Flue-cured, var. NC 82) during the storage was examined according to the initial moisture content(MC) of the tobacco. The initial moisture content of the leaf tobacco was controlled as 12,14,16% after redrying. Those were packed in cardboard boxes and stored in a warehouse. Samples were taken monthly from June of 1988 through August of 1989 and were evaluated for mold development (colony count) and MC. The moisture content change in the leaf tobacco of 12, 14 and 16% initial MC was in the range of 11.1-13.3, 12.8-15.3 and 14.9-16.7% and the mean number of colonies from them were 1.4$\times$102, 1.0$\times$102, and 4.0$\times$102 colonies per gram of tobacco, respectively, however mold-damaged leaf tobacco other than the treatments was occasionally observed to have the colony number as high as 2.3$\times$105 colonies per gram of tobacco. In the meantime, the leaf tobacco was stored at 6 relative humidity levels at $25^{\circ}C$ in laboratory test. Leaf tobacco of 15.0-16.4% MC was maintained without the mold-damage after 80 days of storage whereas those of 19.9, 21.3, 25.5 and 27.1% MC became moldy after 50, 15, 11 and 6 days of storage, respectively.