• Korean Journal of Microbiology
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• v.45 no.4
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• pp.318-323
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• 2009

The aim of this work was to investigate the antibacterial effect of the food-poisoning bacterium, Salmonella enteritidis JK-15 exposed to rose extracts. Initially, the isolate S. enteritidis JK-15 was enriched and isolated from stale food. BIOLOG and 16S rRNA analyses revealed that strain S. enteritidis JK-15 was 98% similar to the S. enteritidis species cluster; therefore we have designated this strain as S. enteritidis JK-15. Bactericidal effects of S. enteritidis JK-15 exposed to rose extracts ranging from 5 mg/ml to 100 mg/ml were monitored, and complete bactericidal effects were achieved within 6 h at 100 mg/ml and 12 h at 50 mg/ml, respectively. SDSPAGE with silver staining revealed that the amount of lipopolysaccharides increased or decreased in the strain S. enteritidis JK-15 treated to different concentrations and exposing periods of rose extracts in exponentially growing cultures. Scanning electron microscopic analysis, demonstrated the presence of irregular rod shapes with umbilicated surfaces for cells treated with rose extracts.

• Current Research on Agriculture and Life Sciences
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• v.32 no.4
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• pp.211-214
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• 2014

This study investigated the effects of using a pollen suspension for artificial pollination on the labor costs, fruit set, and fruit quality attributes of 'Fuji' apples. The pollen germination rate was 20% in a 20% fructose solution after 6 hours, and the pollen remained stable for 6 hours in the same solution. The king fruit per flower cluster exhibited a normal fruit set, regardless of the treatment. Plus, none of the artificial pollination applications affected the fruit length/diameter ratio, flesh firmness, soluble solids concentration, or titratable acidity in the harvested 'Fuji' apple fruits. However, the labor savings were significantly higher with the pollen suspension treatment when compared with the cotton swab or love-touch. Therefore, the labor cost was four-fold less in use of pollen suspension than in use of cotton swab for artificial pollination.

• Korean Journal of Microbiology
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• v.55 no.3
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• pp.306-308
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• 2019

A number of Bacillus strains are known to have antimicrobial activity useful in various fields. In order to prevent Propionibacterium acnes, which is one of the factors of acne, we selected Bacillus halotolerans F41-3 which have high antimicrobial activities against P. acnes. We conducted complete genome sequencing of B. halotolerans F41-3 and analyzed genomic characteristics. This genome size is 4,144,458 bp with a G + C content of 43.76%, 4,145 total genes and 3,686 protein coding genes. Among the genes, we found that gene clulster of subtilosin, a kind of bacteriocin, synthesis and gene cluster of nickel transportation. Both of them may influence inhibition of P. acnes.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.68 no.1
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• pp.8-19
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• 2023

Adzuki beans have gained popularity in recent years due to their health benefits. Breeding of Adzuki beans is less favorable than with other legumes due to low genetic diversity. This study aimed to evaluate the genetic diversity of 252 adzuki bean germplasms from China, Japan, and Korea using 18 agro-morphological parameters and comparing their performance to three prominent Korean cultivars: Geomguseul, Arari, and Chungjupat. Leaf shape, pod color, and seed coat color were among the qualitative traits that showed wide variations. The quantitative variables also showed wide variations among adzuki bean germplasms. Although there was no significant difference (p < 0.05), the average rate of germination declined in the order of Korean (91.44%) > Chinese (91.31%) > Japanese (87.47%) adzuki beans. Chinese adzuki beans needed fewer days to flower (DF, 58.22 days) and days to mature (DM, 107.13 days), which varied significantly compared to the Korean and Japanese adzuki beans (p < 0.05). The average number of pods per plant (PPP) and one-hundred seeds weight (HSW) were higher in Japanese adzuki beans compared to the Korean and Chinese adzuki beans although the variation of each was not significant. Almost 29.76% of the accessions had early-blooming flowers, 3.97% were premature, 21.43% produced more PPP, and 3.97% yielded more SPP compared to control cultivars. Results of hierarchical cluster and principal component analyses revealed three clusters with significant variation in all quantitative variables except for RG (p < 0.05). The key factors in multivariate analyses were DF, DM, and HSW. Our study investigated the genetic diversity of adzuki bean accessions and identified ten early maturing and ten high PPP-yielding accessions. Our findings would help farmers and breeders to select the top-performing accessions that can provide them with various options.

• Horticultural Science & Technology
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• v.34 no.2
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• pp.228-235
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• 2016

This study was conducted to determine the yield of cherry tomato (Solanum lycopersicum Mill.) grown at three planting times during the uncultivated period of strawberry. Cherry tomato was planted under condition filled with strawberry dedicated culture medium on a two-story bed with April 20, April 30, and May 10 at 2015. Fruit harvest was completed on July 31. The supply concentration of nutrient solution at the time of transplanting was started as EC $1.2dS{\cdot}m^{-1}$ and it was gradually increased to EC $2.5dS{\cdot}m^{-1}$ after blooming of the first flower cluster. Netherlands PBG solution was supplied for one minute six times per day. The heights of cherry tomato plants planted at earlier were significantly greater than those of tomato plants planted later. The heights of cherry tomato plants grown at the bottom of the bed were greater than those grown in the upper bed. The yield of cherry tomatoes planted on April 20 at the bottom of the bed was greatest with an average of 2,954 g of tomatoes per plant. There were no significant differences in the average weight and sugar content of fruit according to planting times and bed position. The yield of cherry tomato plants planted on April 20 was 18% and 34% higher than that of plants planted on April 30 and May 10. We confirmed to increase the yield of the cherry tomato when early plants planted on two-story bed. These results indicate that farmers can choose the best period of producing cherry tomato during the un-cultivated period of strawberry under two-story bed conditions.

• Journal of Bio-Environment Control
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• v.24 no.3
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• pp.217-225
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• 2015

Local heating system providing hot air locally to growing parts including shoot apex and flower cluster which were temperature-sensitive organs of cherry tomato was developed to reduce energy consumption for greenhouse heating without decline of crop growth. Growing part following local heating system was composed of double duct distributer which connected inner and outer ducts with hot air heater and winder which moved ducts up and down following growing parts with plant growth. Growing part local heating system was compared with conventional bottom duct heating system with respect to distributions of air and leaf surface temperatures according to height, growth characteristics and energy consumption. By growing part local heating, air temperature around growing part was maintained $0.9{\sim}2.0^{\circ}C$ higher than that of lower part of crop and leaf surface temperature was also stratified according to height. Investigations on crop growth characteristics and crop yield showed no statistically significant difference except for plant height between bottom duct heating and growing part local heating. As a result, the growing part local heating system consumed 23.7% less heating energy than the bottom duct heating system without decrease of crop yield.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.17
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• pp.79-114
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• 1974

Experiments were conducted to clarify the variations of the ecological characteristics under different day-length and temperature conditions inperilla varieties from 1972 to 1973 in the experimental fields of Crop Experiment Station, O.R.D, Suwon. Thirty-six varieties were tested in the field in 1972 under 6 growing seasons differing seeding dates. from April 5th to June 20th with 15-day interval between each seeding. Pot-experiment also were conducted in 1972 and 1973. The seeds of the 6 varieties tested were sown on May 25th. In this pot-experiment natural condition was regarded as a short-day treatment and 100-W incandescent lamps were used for long-day treatment. Three selected varieties were grown under different. temperature treatments in phytotron in Crop Experiment Station. The results obtained are summarized as follows: 1. Most varieties tested flowered around September 6. The days required to flower were shortened gradually as the planting time was delayed. 2. The varieties used were matured around October 6, but the maturity was shortened when planted early. The days required for maturity after flowering was 26 to 30. 3. The growing period was also shortened gradually when planting time was delayed. 4. Plant height was reduced when planting time was delayed. 5. There were little differences in number of valid branches among planting time I, II and III, while the branch number was reduced as the planting time was delayed. 6. The dry matter weight was gradually increased from planting time I to III, while it was rapidly decreased after planting time IV. 7. It was found that the flowering of perilla was little affected by temperature. The varieties, however, were more sensitive to day-length. 8. No clear tendency was found in the plant height, number of valid branches and dry matter weight by the time and period of day-length and temperature treatments. 9. The highest yield was obtained at planting time III(May 5th) and the yield was decreased at either earlier or later planting. 10. 1, 000 grain weight appeared to be heavier as the planting time was delayed. 11. The number of flower cluster was largest at planting time III (May 5th) and it was decreased as planting time was earlier or later than III. 12. The oil content was also highest at planting time III (May 5th). 13. Days to flowering, days to maturity and total growing period and flowering period did not affect the yield much. 14. The number of valid branches, flower clusters, 1, 000-grain weight and dry matter weight were positively correlated with yield. The relationship between these characters and yield were variable depending upon the planting time.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.3
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• pp.248-255
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• 2021

This study was conducted to obtain basal information for the development of perilla cultivars with improved quality. The F₇ population of recombinant inbred lines (RILs) from a cross between the parents of Daesil for seeds and Ipdeulkkae1 for vegetables was used as the material. We evaluated several agricultural characteristics and seed quality. Variations were observed in most of the measurements; for example, stem length (ranging from 66.0 to 150.0 cm), number of branches (from 5 to 23), flower cluster length (ranging from 5.1 to 10.5 cm), number of flower clusters (from 17 to 131), a-linolenic acid content (from 54.2 to 64.1%), and functional compound content (rosmarinic acid 869.5~3,508.1 ㎍/g; luteolin 47.4~864.3 ㎍/g; apigenin 57.1~296.7 ㎍/g) all showed variation. Significant correlations between stem length and the number of branches (0.561) and number of branches versus number of flower clusters (0.638) were detected in the RIL F₇ population. Most agricultural characteristics and seed qualities showed a normal distribution with large variation, and transgressive segregation was observed in many descendants with characteristics to those of their parents. Daesil/Ipdeulkkae1 RIL F₇ populations could be useful for future QTL analysis as well as for intermediate breeding lines for high-quality perilla cultivars.

• Journal of Bio-Environment Control
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• v.22 no.4
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• pp.421-426
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• 2013

This study was conducted to investigate the effects of timing of nutrient starvation during transplant production on growth of runner plants and yield of strawberry 'Seolhyang' (Fragaria ${\times}$ ananassa). Nutrient solution supply at the level of EC (electrical conductivity) 0.8 $dS{\cdot}m^{-1}$ was terminated at interval about 10 days between July 25 and September 5. As a result, the growth of above-ground part was inhibited while root growth increased when the nutrient starvation treatment had been brought forward to July 25. It also reduced the T/R ratio significantly and chlorophyll content was tended to be lower than the other treatment. In addition, it significantly promoted the budding, flowering and harvest of first flower cluster. On the other hand, the period of harvest was delayed more than two weeks when the nutrients were continuously supplied after the middle of August. An accumulated marketable fruit yield per plant until the end of January and February was 169 and 266g, respectively in the treatment of nutrient starvation on July 25, which was 71 and 12% increase, respectively, as compared with those in the treatment of September 5. Therefore, the appropriate nutrient starvation in the late season of strawberry nursery period could be expected the increase in yield and income during the winter season by promoting the flower bud differentiation as reducing the endogenous nitrate level of the plantlet.

• Journal of the Korean earth science society
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• v.26 no.6
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• pp.524-540
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• 2005

This study has focused on the amount of evaporites and geochemical characteritics of evaporites from the acid mine drainage and on the variation of constituents in acid mine drainage during evaporation. The various colors of evaporites are frequently observed at the rock surfaces contacting acid mine drainage. In order to produce evaporites in the laboratory, acid mine drainages were sampled from the abandoned mine areas (GTa, GTb, GH and GB) and air-dried at room temperature. During the evaporation of acid mine drainages, TDS, EC values and the concentrations of major and minor ions increased, whereas ER and DO values decreased with time. The concentration of Fe increased gradually with evaporation time in the GTb and GB, whereas GH founded in one day but rapidly not detected in the other day after due to removal of Fe by formation-precipitation of amorphous Fe hydroxide. The amounts of the evaporites were produced in amounts of 4 g (GTa), 5 g (GB), 15 g (GH), and 24 g (GTb) from 4 liter of acid mine drainage after 80 days of the evaporation, respectively. In linear analysis from the products with the parameters which are the EC, TDS, salinity, ER, DO and pH contents in field, the determination coefficients were 0.98, 0.99, 0.98, 0.88, 0.89, and 0.25 respectively. If we measure the parameters in field, it would be easy to estimate the amount of evaporites in acid mine drainage. Gypsum and epsomite were identified in all of the evaporites by x-ray powder diffraction studies. Evaporite (GTb) was heated at 52, 65, 70, 95, 150, 250, and 350oC for one hour in electrical furnaces. Gypsum, $CaSO_4\cdot1/2H_2O$ and kieserite were identified in the heated evaporite by XRD. With increased heating temperature, the intensity of the peak at $7.66/AA$ (diagnostic peak of gypsum), the peak at 5.59A ($CaSO_4{\cdot}1/2H_2O)$ and the peak at $4.83{\AA}$ (kieserite) decreased in x-ray diffraction due to dehydration. In the SEM and EDS analysis for the evaporite, gypsum of well-crystallized, radiating cluster of fibrous, acicular, and columnar shapes were observed in all samples. Ca was not detected in the EDS analysis of the flower structures of GTb. Because of that, the evaporite with flower structures is thought to be eposmite.