• Journal of Advanced Marine Engineering and Technology
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• 제35권5호
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• pp.582-589
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• 2011

선박에서 배출되는 온실가스를 저감하기 위한 기술로 연료전지 기술이 고려되어지고 있다. 본 연구에서는 메탄을 연료로 사용한 내부개질형 500kW급 고체산화물형 연료전지의 선박 적용을 가정한 연료전지 시스템을 모델링하여 시스템의 구성에 따른 공기, 메탄, 물의 공급 유량 및 시스템 운전 압력이 연료전지 스택의 입구 및 출구에서의 가스 온도, 스택 출력 및 시스템 효율 등에 미치는 영향에 관하여 검토하였다. 그 결과 공기와 메탄의 공급 유량이 연료전지 스택 입구 및 출구 가스 온도에 직접적인 영향을 주었다. 공기와 물의 공급 유량이 증가하면 스택 출력 및 시스템 효율이 증가하고, 메탄의 경우 스택 출력은 증가하나 시스템 효율은 낮아진다.

• Journal of Mechanical Science and Technology
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• 제20권12호
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• pp.2218-2229
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• 2006

A conservative finite-volume numerical method for unstructured grids with the cell-centered method has been developed for computing flow and heat transfer by combining the attractive features of the existing pressure-based procedures with the advances made in unstructured grid techniques. This method uses an integral form of governing equations for arbitrary convex polyhedra. Care is taken in the discretization and solution procedure to avoid formulations that are cell-shape-specific. A collocated variable arrangement formulation is developed, i.e. all dependent variables such as pressure and velocity are stored at cell centers. For both convective and diffusive fluxes the forms superior to both accuracy and stability are particularly adopted and formulated through a systematic study on the existing approximation ones. Gradients required for the evaluation of diffusion fluxes and for second-order-accurate convective operators are computed by using a linear reconstruction based on the divergence theorem. Momentum interpolation is used to prevent the pressure checkerboarding and a segregated solution strategy is adopted to minimize the storage requirements with the pressure-velocity coupling by the SIMPLE algorithm. An algebraic solver using iterative preconditioned conjugate gradient method is used for the solution of linearized equations. The flow analysis code (PowerCFD) developed by the present method is evaluated for its application to several 2-D structured-mesh benchmark problems using a variety of unstructured quadrilateral and triangular meshes. The present flow analysis code by using unstructured grids with the cell-centered method clearly demonstrate the same accuracy and robustness as that for a typical structured mesh.

• 미생물학회지
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• 제55권3호
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• pp.199-205
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• 2019

Yeast two-hybrid는 특정 단백질에 대한 상호작용 파트너 단백질의 선별을 위한 방법으로 개발되었다. 하지만 대규모 단백질 상호작용체 분석을 수행하기에 요구되는 노동과 대량의 한천배지 사용에 따른 문제에 의해 널리 사용되지 못하고 있다. 따라서 본 연구에서는 새로운 리포터 시스템을 yeast two-hybrid 방법에 도입하여 fluorescence-activated cell sorting (FACS) 또는 magnetic-activated cell sorting (MACS)를 이용하여 상호작용 파트너 단백질을 포함하는 효모 클론을 손쉽게 선별할 수 있도록 하였다. 새로운 리포터 시스템은 c-myc 항원 결정기가 총 10번 반복되는 형태로 효모 표면에 발현되도록 하였으며, p53과 SV40 T항원을 이용한 실험을 통하여 리포터 단백질의 정상적인 발현을 flow cytometry 분석을 통하여 확인하였다. 따라서, 새로운 리포터 시스템을 도입한 yeast two-hybrid 방법은 대규모 상호작용체 분석을 위해 필요한 노력을 현저히 줄일 수 있을 것으로 기대한다.

• 신재생에너지
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• 제7권3호
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• pp.29-35
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• 2011

This paper describes an internal flow characteristics of a fuel pressurized blower, used for 1kW domestic fuel cell system. To analyze the flow field inside the diaphragm cavity, compressible unsteady numerical simulation is introduced. SST model with scalable wall function is employed to estimate the eddy viscosity. Moving mesh system is applied to the numerical analysis for describing the volume change of a diaphragm cavity in time. Throughout numerical simulation with the modeling of the inlet and outlet valves in a diaphragm cavity, unsteady nature of an internal flow is successfully analyzed. Force variations on the lower plate of a diaphragm cavity are evaluated in time. It is found that the driving force at the suction stage of a diaphragm cavity is more necessary than that at the discharging stage.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2005년도 추계 학술대회논문집
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• pp.81-85
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• 2005

Three-dimensional steady incompressible laminar entry flows in a square duct of $90^{\circ}$ bend are numerically simulated by a new solution code(PowerCFD) using unstructured cell-centered method. Solutions are obtained with three unstructured grid types of hexahedron, prism and hybrid at a Reynolds number, based on the hydraulic diameter and bulk velocity, of 790. Interesting features of the flow are presented in detail. Detailed comparisons between the computed solutions and the available experimental data are given mainly for the velocity distributions at cross-sections in a $90^{\circ}$ bend of a square duct with fully-developed entry flows. It is found that the code is capable of producing the nature of laminar flow in curved square duct with no grid type dependency.

• Management Science and Financial Engineering
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• 제3권1호
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• pp.15-38
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• 1997

In a flexible manufacturing system, a cell controller is able to identify and evaluate a number of alternative decisions to meet the objectives set by the factory level controller. In this paper, a Petri net-based cell controller is presented to accomplish this task. A static model is developed by using the Integrated Computer Aided Definition(IDEF0) method to represent clear functional relationships among the objects of the system. Based on the static model, two Petri net models are developed for the physical part flow and for the information flow. Multiple decision alternatives are generated from the physical part flow model and are synchronized with the information flow model for execution of the selected alternative.

• Bulletin of the Korean Chemical Society
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• 제28권3호
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• pp.403-407
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• 2007

The anodic degradation of benzoquinone(BQ), a model compound for wastewater treatment was carried out using a home-made flow-through electrochemical cell with carbon fibers. To optimize the controlled current electrolysis condition of an aqueous BQ solution, the experimental variables affecting the degradation of BQ, such as the applying current, pH, reaction time, and flow rate of the BQ solution were examined. The degradation products of the oxidation reaction were identified by High Performance Liquid Chromatography and Inductively Coupled Plasma Atomic Emission Spectrometer. Low molecular weight aliphatic acids, and CO2 were the major products in this experiment. The removal efficiency of BQ from the solution increased with the applying current and time. 99.23% of 1.0 × 10-2 M BQ was degraded to aliphatic acids and CO2 when the applying current is 175 mA in a 12 hr electrolysis.

• 반도체디스플레이기술학회지
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• 제10권2호
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• pp.109-113
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• 2011

Hot air flow simulations of the wet station dryer module for the solar cell cleaning were conducted. Air incident angles such as to the substrates ($45^{\circ}$), to the bottom ($90^{\circ}$), and to the wall ($135^{\circ}$) were considered. Based on the simulated velocity and temperature profiles, appropriate incident angle was proposed, and it was well matched to experimental results. Additionally, uniform and non-uniform air hole sizes of the tube were compared for the uniform air flow distribution through the batch.

• 한국임상수의학회지
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• 제38권1호
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• pp.36-40
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• 2021

A 12-year-old dog was referred due to multiple superficial lymphadenopathy. On cytology, each lymph node showed different cell populations where some of them consisted of intermediate to large lymphocytes with frequent Mott cells. Presence of Mott cells along with immature lymphocytes made the cytological diagnosis challenging, and therefore, supplementary diagnostic tests including PCR for Antigen Receptor Rearrangement (PARR) assay and flow cytometry were performed. This case report illustrates the value of flow cytometry in the diagnosis of lymphadenopathy with ambiguous cytologic findings.

• Journal of Mechanical Science and Technology
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• 제14권8호
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• pp.861-866
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• 2000

Flow induced shape change is important for spatial interpretation of vascular response and for understanding of mechanotransduction in a single cell. We investigated the possible shapes of endothelial cell (EC) in a mathematical model and compared these with experimental results. The linearized analytic solution from the sinusoidal wavy wall and Stokes flow was applied with the constraint of EC volume. The three dimensional structure of the human umbilical vein endothelial cell was visualized in static culture or after various durations of shear stress (20 $dyne/cm^2$ for 5, 10, 20, 40, 60, 120min). The shape ratio (width: length: height) of model agreed with that of the experimental result, which represented the drag force minimizing shape of stream-lining. EC would be streamlined in order to accommodate to the shear flow environmented by active reconstruction of cytoskeletons and membranes through a drag force the sensing mechanism.